BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,501	C→T	L371L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,501	0	C	T	96.9%	408.0 / NA	127	L371L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (62/61); minor base G (0/4); total (62/65)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 127 total aligned reads displayed.

ATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCG  >  NC_000913/23256‑23747

aTTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCCACGTTCCGAAAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAACGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGAt                                                                                                                                                                                                                                                   >  1:362313/1‑251 (MQ=255)

  tACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATg                                                                                                                                                                                                                                                  <  1:383779/250‑1 (MQ=255)

    cacCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTACGACGTTCCGGCAATCCACGGCGATCACGGTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATCCGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCg                                                                                                                                                                                                                                               <  1:43652/251‑1 (MQ=255)

      ccATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCGCTTATCCGACGTTGGATGGCGTg                                                                                                                                                                                                                                             >  2:9137/1‑251 (MQ=255)

             cGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGt                                                                                                                                                                                                                                        <  2:317608/249‑1 (MQ=255)

              ggCACGGTACAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCAACCGATTATGGGCTTCGACGTTCCGGCAATCCTCGGCGCTCACGTTACACTGGCTGCCGGAACCGGCGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGGCTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGACCGGACGGCACTTATCTGCCGGGCCCTTATCCGCCGTTGGATGGCGTGAACGTCtt                                                                                                                                                                                                                                     >  1:544759/1‑251 (MQ=255)

                 aCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCa                                                                                                                                                                                                                                   >  1:440775/1‑250 (MQ=255)

                   ggTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCaa                                                                                                                                                                                                                                  >  1:41367/1‑249 (MQ=255)

                    gTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATTCGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGc                                                                                                                                                                                                                               <  2:107212/251‑1 (MQ=255)

                       aaaGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGAGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAACGCGa                                                                                                                                                                                                                             >  2:394699/1‑250 (MQ=255)

                          ggTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGACTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGa                                                                                                                                                                                                                         <  1:407779/251‑1 (MQ=255)

                           gTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGAc                                                                                                                                                                                                                        >  1:205848/1‑251 (MQ=255)

                            tGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGAc                                                                                                                                                                                                                        >  1:508061/1‑250 (MQ=255)

                               ggAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtcg                                                                                                                                                                                                                    >  1:194713/1‑251 (MQ=255)

                                      gAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTg                                                                                                                                                                                                              <  1:192343/250‑1 (MQ=255)

                                                gcTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCtgctgc                                                                                                                                                                                                     >  1:59026/1‑249 (MQ=255)

                                                  tttACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAgg                                                                                                                                                                                                  >  1:426226/1‑250 (MQ=255)

                                                   ttACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGa                                                                                                                                                                                                 >  1:586002/1‑250 (MQ=255)

                                                   ttACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGa                                                                                                                                                                                                 >  1:462465/1‑250 (MQ=255)

                                                     aCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGaaaa                                                                                                                                                                                              <  1:29535/251‑1 (MQ=255)

                                                      cccATCCGTTTATGGGCGTCGACGGTCCGCCAATCCTCGGCGATCACGGTACCCTGGATGCCGGTACCGGTGCCGGTCACACCGCGCCTGGCCACAGCCCGGACGACGATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCTGTTGGCCCGGACGGCACTTATCTGCCGGTCACTTATCCGGCGTGGGATGGCGTGAACGTCTTCAAAGCTAACGACAGCGTCGGTGCGCTGCTGCAGGaaaaa                                                                                                                                                                                             <  2:529817/251‑1 (MQ=255)

                                                                tATGGGCTTCGACGTTCCGGCAATCCGCGGCGATCACGTTACCATGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCgctg                                                                                                                                                                                    <  1:339926/250‑1 (MQ=255)

                                                                 aTGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCGGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCgctgct                                                                                                                                                                                  <  1:222482/251‑1 (MQ=255)

                                                                  tGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGCACGACATCGTCGTTGCGCTGCTGCAGGACAAAGGCGCGctgctg                                                                                                                                                                                 >  1:200070/1‑251 (MQ=255)

                                                                  ggggCTTCGACGTTCCGGCAATCCGCGGCGATCACGTTTCCCTGGATGTCGGGACCGGTGCCTGTCACACCGCGCCGGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGTACGGCACTTATCTGCCGGGCACTTATCCGACTGTGGATGGCGTTAACGTCTGCAAAGTGAACGACATAGTCGTTGTTCTGCGGTAGGTATAATGCGCGctgctg                                                                                                                                                                                 <  2:59499/250‑1 (MQ=255)

                                                                   gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCtgctgc                                                                                                                                                                                >  1:310566/1‑251 (MQ=255)

                                                                    ggcctagACGTTCCGGCAATCCGCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCGGGCCACGGCCCGGACGAGTATGTGATCGGTCAGAAATACGTCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATATGCCGGGAACTTATCCGACGTTGGATGGCGTGAACGTCTTTAAAGCGTACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCa                                                                                                                                                                               <  2:261810/245‑1 (MQ=255)

                                                                          gccGTTCCGGCCCTCCTCGGCGTTCCCGTTTCTCTGGAGGCCGCTACCGGTGCCTTTCCCCCCGCGCCTGGCCACGGCCCTGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACGTATCTGCCGGGCACTTATCCGCCGTTGGATGGCGTGAACGTCTGCAAAGCGAACGTCATCGTCGGTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTga                                                                                                                                                                         <  2:74790/249‑1 (MQ=255)

                                                                           aCGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCACAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTg                                                                                                                                                                          >  1:108945/1‑249 (MQ=255)

                                                                                ccGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAAt                                                                                                                                                                   >  2:518550/1‑251 (MQ=255)

                                                                                  ggCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGCTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGGTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATgc                                                                                                                                                                 <  1:79254/251‑1 (MQ=255)

                                                                                   gCAATCCTAGGCACTCACGTGACCATGGATACCGATAACGATGCCGATCACACCGCGCATGGCCAAGGCCCGGACGGCTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGAACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCATCAAAGCGAACAACATCGTCGTTGCGCTGCTGCAGGAAAAAGGAGCGCTGCGGATCGTTGAGAACATgcg                                                                                                                                                                >  2:544234/1‑250 (MQ=255)

                                                                                     aaTCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATgcagc                                                                                                                                                              <  1:341006/251‑1 (MQ=255)

                                                                                         cTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGAAGcac                                                                                                                                                            >  1:179506/1‑249 (MQ=255)

                                                                                           cGGCGATCACGTTCCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCt                                                                                                                                                        <  1:119867/251‑1 (MQ=255)

                                                                                               gATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATcc                                                                                                                                                    <  2:327311/251‑1 (MQ=255)

                                                                                               gATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGAGATCGGGCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGACCGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCCGCTGCACGTTTAGAAAATGCAGCACAGCTATcc                                                                                                                                                    >  2:168270/1‑251 (MQ=255)

                                                                                                  cACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGtg                                                                                                                                                 <  1:413660/251‑1 (MQ=255)

                                                                                                  cACGTTACCCGGGATGCCGGTACCGTTGCCGTTCACACCGCGCCGGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTACCCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGt                                                                                                                                                  <  2:485969/250‑1 (MQ=255)

                                                                                                    cggttcccTGGTTGCCGGTACCGGTGCCGTTCACCCCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAACCCGCTAACCCGGTTGGCACGGACGGCTCTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCGTCAAAGCGAACGACATCGGCGTTGCGCTTCTGCAGGAAAAAGGCGCGCTGCGGCACGTTGAGAAAATGCAGCACAGCTATCCGtgct                                                                                                                                               <  2:277103/246‑1 (MQ=255)

                                                                                                    cGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGtgct                                                                                                                                               <  2:426226/251‑1 (MQ=255)

                                                                                                        ccccTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGctgctg                                                                                                                                           <  1:838/250‑1 (MQ=255)

                                                                                                          ccTGGATGCCGGTACCGGTTCCTTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGc                                                                                                                                         <  2:366281/251‑1 (MQ=255)

                                                                                                                  ccGGTACCGGTGCCGTTAACACAGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACAGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCACAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACaa                                                                                                                                  >  2:537879/1‑250 (MQ=255)

                                                                                                                   cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACaaaa                                                                                                                                <  2:583174/251‑1 (MQ=255)

                                                                                                                   cGGTACCGGTGACGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGATATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACaaa                                                                                                                                 >  1:137174/1‑250 (MQ=255)

                                                                                                                          ggTGCCTTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTGATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTTCGCTGCTGCATGAAAAAGGCGCGCTGCTGCACGTTGAGAAAAGGCAGCACAGATATCCGTGCTGCTGGCGTCTCAAAACGCCGat                                                                                                                         <  2:497958/251‑1 (MQ=255)

                                                                                                                          ggTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGat                                                                                                                         >  1:457533/1‑251 (MQ=255)

                                                                                                                                gTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGCCGGCACTTATCTGCCGGGCACTTATCCGACGTTGGAGGGCGTGACCGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCtt                                                                                                                   >  2:282990/1‑251 (MQ=255)

                                                                                                                                gTTCACACCGCGACGGGCCACGGCCCGGACGACTATGTGATCGGACAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGACCGAACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCGTCAAAGCGAACGACATCGTCGTTGCGCTGCCGCAGGAAAAAAGCGAGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGATGCTGGCGTCACAAAACGCCGATCCTCtt                                                                                                                   >  2:405091/1‑251 (MQ=255)

                                                                                                                                    acacCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGctgctg                                                                                                                                                                                 <  2:307892/185‑1 (MQ=255)

                                                                                                                                    acacCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGctgctg                                                                                                                                                                                 >  1:307892/1‑185 (MQ=255)

                                                                                                                                      acCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCcgcgc                                                                                                             >  1:202509/1‑251 (MQ=255)

                                                                                                                                       ccGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCcgcgcg                                                                                                            <  1:518550/251‑1 (MQ=255)

                                                                                                                                       ccGCGCCTGGCCACGGCACGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCcgcgcg                                                                                                            <  2:575911/251‑1 (MQ=255)

                                                                                                                                         gcgcCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAc                                                                                                          <  1:305533/251‑1 (MQ=255)

                                                                                                                                          cgcCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcg                                                                                                         <  2:205848/251‑1 (MQ=255)

                                                                                                                                               ggCCACGGCCCGGACTACTATGTGAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCTCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCa                                                                                                    <  1:87998/251‑1 (MQ=255)

                                                                                                                                                 ccACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCGGGACACCGCTAACCCGGTTTGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGTCGTGAACGTCTTCAAAGCGAACGACATCATCGATGCGCTGCTGCAGGCACAAGGCGCGCTGCTGAACGTTGAGAAAATGCAGCACAGCTATCCCGACTGCTGGCGGCACAAAACGCCGATAATCATCcgcgttccgccgcggg                                                                                                  >  2:131397/1‑239 (MQ=255)

                                                                                                                                                  cACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTg                                                                                                 <  1:488325/251‑1 (MQ=255)

                                                                                                                                                     ggCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAGCCCGGTTGGCCCGGACGGCACTTATCTGCCTGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCTCTGCTGCGCGTTGAGAAAAGGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGtt                                                                                              <  1:582774/251‑1 (MQ=255)

                                                                                                                                                       cccGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGAAGTGGTTCg                                                                                            >  1:431936/1‑251 (MQ=255)

                                                                                                                                                               acTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAGCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATg                                                                                    <  2:508061/251‑1 (MQ=255)

                                                                                                                                                                  aTGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGGTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATgg                                                                                   <  1:51336/249‑1 (MQ=255)

                                                                                                                                                                    gtgATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCa                                                                               <  1:60573/251‑1 (MQ=255)

                                                                                                                                                                    gtgATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGGTGGATGGCGCGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATc                                                                                <  1:118706/250‑1 (MQ=255)

                                                                                                                                                                     ggATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTGGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCGTCCGCTCGACGCCGCAGTGGTTCGTCAGTATGGATCAg                                                                              <  2:259053/250‑1 (MQ=255)

                                                                                                                                                                        tCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGaa                                                                            >  1:181566/1‑250 (MQ=255)

                                                                                                                                                                           gTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGt                                                                        >  1:329110/1‑251 (MQ=255)

                                                                                                                                                                             cAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTc                                                                       <  2:361907/250‑1 (MQ=255)

                                                                                                                                                                               gAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAGCGTCGGTGCGCTGCTGCAGGAAAAAGGCGCGGTGCTGCACGTTGAGAAAATGCAGCACAGCTAGCCGTGCTGCTGGCTTCACAAAACGCCGATCATCTTCCGCGCGACGTCGCAGTGGTTCGTCAGCATGGATCAGAAAgtcctgc                                                                    <  2:257531/251‑7 (MQ=255)

                                                                                                                                                                                  aTACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTTCGGGCGTCACAAAACTCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCACAAAGGTCtgcgtg                                                                 <  2:582060/251‑1 (MQ=255)

                                                                                                                                                                                   tACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCtgcgtgc                                                                >  1:472550/1‑251 (MQ=255)

                                                                                                                                                                                       gCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGTTATCCGTGCTGCTGGCGTCACAAAAAGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAg                                                            <  1:409348/251‑1 (MQ=255)

                                                                                                                                                                                        ccTGGAAACCGCTAACCCGGTTGGCCCGGACAGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGt                                                           >  1:338391/1‑251 (MQ=255)

                                                                                                                                                                                         cTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGCCGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCCCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTc                                                          >  2:381049/1‑251 (MQ=255)

                                                                                                                                                                                          tGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCGGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGGTCGTCAGCATGGATCAGACAGGTCTGCGTGCGCAGTc                                                          >  1:541880/1‑250 (MQ=255)

                                                                                                                                                                                           ggAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCAc                                                        >  1:258459/1‑251 (MQ=255)

                                                                                                                                                                                              aaCCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTg                                                      >  2:220641/1‑250 (MQ=255)

                                                                                                                                                                                               aCCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGaa                                                    <  2:131128/251‑1 (MQ=255)

                                                                                                                                                                                                  gCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCTGGCACTTATCCGACGTTGGATGGCGTGAACGGCTTCAAAGCGAACTACAGCGTCGTTGCGCTGCTGCAGGAAAAAGGGGCGCTGTTGCACGGGGAGAAAATGCAGCATAGCGATCCGTGCTGCTTGCGTTACTAAGGGCCGATCAGCTTCCGCGCGACGCCGCAGTGTTGCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAaga                                                 <  1:527235/251‑1 (MQ=255)

                                                                                                                                                                                                  gCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCGTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCAGCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAaga                                                 <  2:394113/251‑1 (MQ=255)

                                                                                                                                                                                                  gCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGGGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAaga                                                 <  2:182815/251‑1 (MQ=255)

                                                                                                                                                                                                   cTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGACAGGTCTGCGTGCGCAGTCACTGAAag                                                  >  2:530393/1‑249 (MQ=255)

                                                                                                                                                                                                   cTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGACGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAag                                                  >  2:505537/1‑249 (MQ=255)

                                                                                                                                                                                                      aCCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAag                                                  >  2:301262/1‑246 (MQ=255)

                                                                                                                                                                                                        ccGGTTGGCCCCTGCGGCACTTATCTGCCGGGCACTTATCCGACTTTGGATGGCGTGAACGTCGTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACTGTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTGCGACAGCATGGAGCAGAAAGGTCTTCGTGCGCAGTCACTGAAATAGATCaa                                           <  2:82238/251‑1 (MQ=255)

                                                                                                                                                                                                           gTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTg                                                      <  2:472620/237‑1 (MQ=255)

                                                                                                                                                                                                           gTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTg                                                      >  1:472620/1‑237 (MQ=255)

                                                                                                                                                                                                              ggCCCGGCCGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGt                                     >  1:370713/1‑251 (MQ=255)

                                                                                                                                                                                                              ggCCCGGACGGCACTTATCTGACGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGCCGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGt                                     >  2:271141/1‑251 (MQ=255)

                                                                                                                                                                                                                   ggACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCGGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGt                                <  2:215637/251‑1 (MQ=255)

                                                                                                                                                                                                                       ggCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGAt                            >  1:19991/1‑251 (MQ=255)

                                                                                                                                                                                                                              aTCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGa                      <  2:462465/250‑1 (MQ=255)

                                                                                                                                                                                                                                  gCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGAcg                    <  2:438376/248‑2 (MQ=255)

                                                                                                                                                                                                                                   ccGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTgggg                >  2:424729/1‑251 (MQ=255)

                                                                                                                                                                                                                                    cGGGCACTTATCCGACGTTGGCTAGCGTGACCGTCTTCCAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGCCGCCGCAGGGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGc               >  2:381161/1‑251 (MQ=255)

                                                                                                                                                                                                                                       gCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAg            <  2:310566/251‑1 (MQ=255)

                                                                                                                                                                                                                                               ccGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGctgctg                                                                                                                                           <  2:417838/116‑1 (MQ=255)

                                                                                                                                                                                                                                                cGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCTGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCCGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCAGGACTGGGGCCAGGCGCGTATc   >  1:236783/1‑251 (MQ=255)

ATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCG  >  NC_000913/23256‑23747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`