| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,418,862 | C→T | G255D (GGT→GAT) | ulaG ← | L‑ascorbate‑6‑phosphate lactonase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,418,862 | 0 | C | T | 98.6% | 228.8 / NA | 73 | G255D (GGT→GAT) | ulaG | L‑ascorbate‑6‑phosphate lactonase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (38/34); total (39/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
GATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATG > NC_000913/4418623‑4419104 | gATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGttt < 2:218036/251‑1 (MQ=255) tGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATAGCCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGcc > 1:405153/1‑251 (MQ=255) ggTACTCTAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATACCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGcc < 2:489776/250‑1 (MQ=255) tACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGt > 1:192115/1‑250 (MQ=255) gAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATc > 1:276020/1‑251 (MQ=255) gTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCt > 2:381167/1‑250 (MQ=255) gTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACg > 1:431255/1‑251 (MQ=255) cTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCCCAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGcc > 2:527979/1‑251 (MQ=255) tATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTTACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACg > 2:158354/1‑251 (MQ=255) ccAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTc < 1:381167/250‑1 (MQ=255) cAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGa < 2:165234/251‑1 (MQ=255) gCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGGTTTCGCCGTACGATCCTAACGCCACGTCGATCTg > 1:37789/1‑250 (MQ=255) gCCAGGTAAAGTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGAACACTACGTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGa < 2:320305/251‑1 (MQ=255) ccAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGAt < 1:457857/251‑1 (MQ=255) ccAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGAt > 1:99461/1‑251 (MQ=255) ggTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGCCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGtt > 2:168887/1‑251 (MQ=255) aaaTTTGCCACCCACATGCCAGATAAACGGCTTGACGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCCCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGt > 2:92119/1‑251 (MQ=255) ccacccacCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTg > 1:399040/1‑251 (MQ=255) gCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGACGATCTGATGTTCGTTACCGTGCTTCGCataa < 2:101020/251‑1 (MQ=255) ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTAGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCctaat > 1:262476/1‑251 (MQ=255) cTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGagtagt > 2:555160/1‑251 (MQ=255) gAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGGCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTggg < 1:285269/251‑1 (MQ=255) cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcg < 1:122416/251‑1 (MQ=255) tACTTCAGGCGATCTTTTTAAATCTCCCACAGCACGCGAATATCTTGCGGCTCGGCCTGGAAGTTTGCCCAGATATCGTGGTGGACCGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCCCTGTTCCTTTTGTAGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGACACGTCGATCTGCTGTTCGTTTCCGTGCTTCGCCTAATAGTTAGAGTCGTGGGAGTcgcc > 2:155830/1‑251 (MQ=255) tCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCt < 1:85943/250‑1 (MQ=255) cgctCTTTTTTCATCTCCCACAGCTCTAGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGCTATCGTGGTGGAACGGGATCACTCCTTTCGCATTCCGCGCTTCACCCATACGCAGAATATCGGCGCTGGTCATTTTGTCGGTGATCTCGCGCGGGTTTTCGCCGTATGATCCTAACGCCACGTCGCTCTGATGTTCGTTCCCGTGCTTCGCATATTAGTTAGTGTAGTTGTGGTCGCCGCTGTGAt > 2:194839/4‑250 (MQ=255) gATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGGTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATAc > 1:38846/1‑251 (MQ=255) tCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATAc > 1:262446/1‑249 (MQ=255) cTTTTTTCAACTAACACAGCCCGCGAATCAATTGCGGACCGGACTGGAAGTTTGACCAGAAATCGTGGTGGAACCGGCTCAATAATTTCGCATTCAGCGCTTAACCCATACGCAGCCTATCGGCGCTGGTCATTTTGTCGGTGATCTCACGCGGGTTTTCGCCGCACGATCCTCACGCCACGTCGATCTGATGTTCGTTACCGTGCTTAGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAgg > 2:273046/1‑251 (MQ=255) tttttCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATa < 1:528402/245‑1 (MQ=255) cccACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgccgcc < 1:558167/247‑1 (MQ=255) cacaGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGt > 1:327249/1‑251 (MQ=255) acaGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtt < 2:192115/251‑1 (MQ=255) caGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGttt > 1:237118/1‑251 (MQ=255) cACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGCTCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTg > 2:328387/1‑250 (MQ=255) ggATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGCTACAGGGAGCCGCCAGGCGTTTTGAACAgg > 2:478505/1‑251 (MQ=255) tctcTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGt > 2:529018/1‑249 (MQ=255) gCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCAcc < 1:50625/251‑1 (MQ=255) gCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCAcc < 1:210320/251‑1 (MQ=255) ggCCTGGAAGTTTGACCAGAGATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGAt < 2:15960/251‑1 (MQ=255) ccTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCg > 1:366640/1‑251 (MQ=255) ggAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTc < 2:237118/250‑1 (MQ=255) aGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGCGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATg > 1:559934/1‑251 (MQ=255) aGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATg < 1:387757/251‑1 (MQ=255) gACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGCTCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTAGCCGCTGCGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATc > 1:281934/1‑251 (MQ=255) aCCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCt < 1:545202/251‑1 (MQ=255) cAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTgg > 1:438725/1‑251 (MQ=255) aGATATCGTGGTGGAAAGGGATCACTACTTTCGCATTCAGAGCTTAACCCATACGCAGCATATCGGCGCTGCTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGCTCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGACCAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGc > 2:570101/1‑251 (MQ=255) cGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGGAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACg < 2:366640/251‑1 (MQ=255) gtggtgGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCAGGCAGTACGc > 2:395962/1‑251 (MQ=255) tCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTAGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTg > 2:291321/1‑250 (MQ=255) ctTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCggc > 2:532557/1‑250 (MQ=255) cGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCggcaggc > 2:284093/1‑250 (MQ=255) cGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCggcaggc < 1:291321/250‑1 (MQ=255) aTTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTTATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTCCGCCAGCCGCTTTTTGATCGGCAGGCAggg < 1:231195/251‑1 (MQ=255) cAGCGCTTCACCCATCCGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGa < 2:37789/251‑1 (MQ=255) ttCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTg > 2:314957/1‑251 (MQ=255) ccATACGCAGCATAGCGGCGCTGGTCATTTTGGCGGTGATATCGCGCGGGTTTTCGCCGTACGAGCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGAGCGGCAGGCAGGGTGATCAGTGCAGTa < 2:276020/251‑1 (MQ=255) cATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTAc < 2:99461/251‑1 (MQ=255) agtcGCAGCTTATCGACGCTGGTCATTTTGTCGTTGATATCGCGCGGGTTTTCGCCGTACTATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGATTCGCATAATAGTTAGGGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACg < 2:313290/248‑1 (MQ=255) caTATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGaa > 1:285527/1‑249 (MQ=255) atatCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGc < 1:555160/251‑1 (MQ=255) atCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCttt < 2:366660/207‑1 (MQ=255) atCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCttt > 1:366660/1‑207 (MQ=255) atCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCa < 1:521957/250‑1 (MQ=255) atCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCAGCTGGCAGTACGCCAGCCGCTTTGTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCa < 1:475900/250‑1 (MQ=255) atCGGCGATGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGACCCTAACGCCACGTCGATCTGATGTTCGTTCCAGCGCTTCGCATCCTAGCTAGAGTAGTGGGTGTCCCCGCTGTCATCCAGGGAGCCGCCCGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCAATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCCAGGTGATTAGTGCAGTACGGTCGAAAGCAt > 2:574962/1‑251 (MQ=255) ccGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTCCCGTGCTTCGCATAATAGTTAGAGTTGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGTCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATc < 2:121550/250‑1 (MQ=255) gcgcTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGCTCGGCAGGCAGGGGGCTCAGTGCAGTACGGTCGCAAGCATCa > 2:543342/1‑249 (MQ=255) gcTGGTCATTTTTGCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAgcg < 2:431255/251‑1 (MQ=255) tGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTc < 2:399040/250‑1 (MQ=255) tGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTc < 2:262446/250‑1 (MQ=255) cGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATg > 1:447618/1‑251 (MQ=255) | GATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATG > NC_000913/4418623‑4419104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |