Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,373,421	C→T	A272T (GCG→ACG)	yjeJ ←	protein YjeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,373,421	0	C	T	100.0%	207.9 / NA	67	A272T (GCG→ACG)	yjeJ	protein YjeJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/38);  total (29/38)

GGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGA  >  NC_000913/4373178‑4373660                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gggTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAg                                                                                                                                                                                                                                          <  1:125620/251‑1 (MQ=255) gggTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACAGAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAg                                                                                                                                                                                                                                          <  1:394963/251‑1 (MQ=255)              ataATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGTATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGGGATGCAGCGTGCGTAAGCATTGATCCTgg                                                                                                                                                                                                                              <  2:516761/250‑1 (MQ=255)              ataATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCGGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTggg                                                                                                                                                                                                                             >  1:487267/1‑251 (MQ=255)                aaTTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCTCGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGtt                                                                                                                                                                                                                           <  1:558097/251‑1 (MQ=255)                        gATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTg                                                                                                                                                                                                                   >  2:326013/1‑251 (MQ=255)                        gATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGGCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGtt                                                                                                                                                                                                                    <  1:495313/250‑1 (MQ=255)                        gATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATGACCAACGATCACGTTAGCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGtt                                                                                                                                                                                                                    <  1:356490/250‑1 (MQ=255)                        gATGCGCGTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATGTCGCCTGTGGTGCAGTTGTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTg                                                                                                                                                                                                                   <  2:58852/251‑1 (MQ=255)                            cgcttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATACGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGAGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCg                                                                                                                                                                                                               >  1:253874/1‑251 (MQ=255)                              cttcgcttATCACGCCTACATAAGGCATTGCACCTGATAGAA‑TACTTGCGAATTTATAGGCCGGATAAGGCGTTTCCGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTAGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGGGATGCCGCGTGAGTAAGCATTGATCCTGGGTTAGCGGATggcagtt                                                                                                                                                                                                             >  2:52105/1‑246 (MQ=255)                               ttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGtt                                                                                                                                                                                                             >  2:511837/1‑250 (MQ=255)                                tcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGtt                                                                                                                                                                                                             <  1:314339/249‑1 (MQ=255)                                 cgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTAtt                                                                                                                                                                                                          >  2:369614/1‑251 (MQ=255)                                      aTCATGCCTACATAAGGCATTGCATCTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACTCGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGTCGTTATTcgccg                                                                                                                                                                                                     <  1:422266/251‑1 (MQ=255)                                         aGGCCAACGTAAGGCATTGCAACGGATTGAACTG‑TTGAGAATTTGTAGGCCGGATAAGGCGGTTTCGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGTGGGTTGGCGCCGGCCGCTCATGAGCAAAGATGAGATTAGTCCGCCGGTGGTGCAGTGTTTCTGGTGGAGTGAACACGGAAGGGATGCAGCGTGCGTGAGCAGTGATCCTGGGTTAGCGGTTGTGCGTTATTCGCCGCTa                                                                                                                                                                                                  <  2:520233/251‑1 (MQ=255)                                           gCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACg                                                                                                                                                                                                <  1:11831/251‑1 (MQ=255)                                            ccTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCGGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTGCGCCTGTGGTGCAGTTTGGCTGGGGGATTGAACTCGTAATTGATGCAGCGTGCGTAAGCATTGATCGTGGGTTAGCGGTTGTGCGGTATTCGCGGCTACgg                                                                                                                                                                                               <  2:531022/251‑1 (MQ=255)                                                 aTAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGa                                                                                                                                                                                          <  1:336466/251‑1 (MQ=255)                                                 aTAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGa                                                                                                                                                                                          <  1:178329/251‑1 (MQ=255)                                                     ggCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATGAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCAGTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACAt                                                                                                                                                                                      <  2:408295/251‑1 (MQ=255)                                                      gCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACAt                                                                                                                                                                                      >  2:320941/1‑250 (MQ=255)                                                       cATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGACGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACAt                                                                                                                                                                                      >  2:472939/1‑249 (MQ=255)                                                            cAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTg                                                                                                                                                                               <  1:74754/251‑1 (MQ=255)                                                                 gATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACAt                                                                                                                                                                           <  2:177979/250‑1 (MQ=255)                                                                  aTTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATgg                                                                                                                                                                         <  1:274490/251‑1 (MQ=255)                                                                          gCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACAccc                                                                                                                                                                  >  2:502498/1‑251 (MQ=255)                                                                                 aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGtt                                                                                                                                                           <  2:119694/251‑1 (MQ=255)                                                                                 aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGtt                                                                                                                                                           <  1:281206/251‑1 (MQ=255)                                                                                       tAGGCCGGATAAGGCGTTTACGCCGAATACGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTc                                                                                                                                                      >  2:545161/1‑250 (MQ=255)                                                                                                tAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGc                                                                                                                                            >  2:366203/1‑251 (MQ=255)                                                                                                 aaGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCt                                                                                                                                           <  1:509137/251‑1 (MQ=255)                                                                                                  cggCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTg                                                                                                                                          >  2:67275/2‑251 (MQ=255)                                                                                                      gTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTGTTTTCAGTCTTGGGCTGAAAt                                                                                                                                      <  2:583879/251‑1 (MQ=255)                                                                                                         tACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCCGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGCTTATTTCTCCTGTGGTGCAGTTTTGCTGGTGGATGGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACATCCGCTAGTTTTTGCAGTCGTGGGCTGAAATCa                                                                                                                                    <  2:394546/250‑1 (MQ=255)                                                                                                          aCGCCGCATCCGGCATGAACAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCGCCAGCCTCGCAGTAGCAAAGATGAGATTATTGCGCCTGTGGTGCAGTGTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATACGCCGCTACGGTGGGAACATAGACATGACATGGCACGCCAGCGAGTTTTTGCAGTCGTGGGCTGAAATCaa                                                                                                                                   <  2:188316/250‑1 (MQ=255)                                                                                                                 aTCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACCCGTAAGTGATGCAGCGTGCGTAAGCATTGCTCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTAGGCTGAAATCAAGCATTCgg                                                                                                                           >  1:257850/1‑251 (MQ=255)                                                                                                                      gaaTGACCAACGATCACGTTATCCGTCATTTAAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGGGGATTGAAAACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGATAAGGGGCGAACATAGACCTGACATGGCAAACCCGCGAGTTTTTTCAGTCGTGGGCGGAAATCAAGCCATCGGCGAGt                                                                                                                      >  1:319127/3‑251 (MQ=255)                                                                                                                      gCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGt                                                                                                                      >  1:319120/1‑251 (MQ=255)                                                                                                                       cATGACCAACTATCACGTTATCAGTCATTTCAGGCTGGATGGCGCCAGCCGCGCATTAGCAAAGATGAGAGTATTTCGCCGGTGGTGCAGTTTTGCTGGTGGAGTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCTGTGGGGCGTTAGTCGCCGCTACGGTGCGAACATAGACCTGACATGGGACTCCCGCGAGTTTTTGCAGTCGTGGGCTGAAATCAAGCAGTCGGCGAGTg                                                                                                                     <  2:66752/251‑1 (MQ=255)                                                                                                                        aTGACCAACGAGCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTGTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGa                                                                                                                    <  2:262383/251‑1 (MQ=255)                                                                                                                         tGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGAt                                                                                                                   >  1:134032/1‑251 (MQ=255)                                                                                                                          gACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGAt                                                                                                                   <  1:20897/250‑1 (MQ=255)                                                                                                                           aCCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCg                                                                                                                 <  1:369614/251‑1 (MQ=255)                                                                                                                                  aTCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTAt                                                                                                          >  1:28061/1‑251 (MQ=255)                                                                                                                                  aTCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTAt                                                                                                          <  1:445056/251‑1 (MQ=255)                                                                                                                                       ggtATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGACTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCAGTGATCCTGGGTTAGCGGTTGGGCGTTATTCGACGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGGGTGATCGCTTCTATTTCtt                                                                                                     <  2:212346/249‑1 (MQ=255)                                                                                                                                       gTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCtt                                                                                                     <  1:470739/251‑1 (MQ=255)                                                                                                                                        ttATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCttt                                                                                                    >  1:25765/1‑251 (MQ=255)                                                                                                                                         tATCAGTCATTTCAGGCTGGATGTCTCCTGCCTCGCATTAGCATAGATTAGATTATTTCGCCTGTGGTGCAGTTTTTCTGGTGTATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGTACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCGGAAATCAAGCATTCGTCGTGTGATCGCTTCTATTTCttt                                                                                                    <  2:499085/250‑1 (MQ=255)                                                                                                                                             aGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGGTGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCg                                                                                               <  1:326013/251‑1 (MQ=255)                                                                                                                                                     cAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCt                                                                                       <  2:371346/251‑1 (MQ=255)                                                                                                                                                           ggATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTGGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTTCGAACATAGACCTGACATGGCACTCCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCTCTGCTATTTCGTTACCCGGCGTGGCTTCAc                                                                                   <  1:153924/249‑1 (MQ=255)                                                                                                                                                                gCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGtttt                                                                            >  1:316605/1‑251 (MQ=255)                                                                                                                                                                      gCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTcaccac                                                                      >  1:217595/1‑251 (MQ=255)                                                                                                                                                                       ccTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACt                                                                     >  1:531681/1‑251 (MQ=255)                                                                                                                                                                                                    gcgtgtgGGGCAGTTTTGGTGGTGGATTGAACTCGGAAGTGATGTAGCGTGCGTAAGCATTGATCCTGGGTTAGTGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGTCCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGGTTCACGCGTTGTCACCACTGCGCCTCTAAATTGTTCTTTGCCACTTTc                                        <  2:578153/248‑1 (MQ=255)                                                                                                                                                                                                    gCCTGTGGTGCAGTTTTGCTGGGGGATTGAACACGTAAGTGATGCAGCGTGCGCCAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGACGCTACGGTGCGAACATAGACCTGAAATGGCACACACGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTATTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTc                                        >  2:528855/1‑251 (MQ=255)                                                                                                                                                                                                        gtggtgCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCt                                    >  2:473317/1‑251 (MQ=255)                                                                                                                                                                                                                    tGCTGGTGGATTGAACACGTAAGGGATGCAGCGTGCGTAAGCATTGATCCTGGGTTCGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGAGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCTCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTa                         >  2:130746/1‑250 (MQ=255)                                                                                                                                                                                                                        ggtggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCa                    <  2:316605/251‑1 (MQ=255)                                                                                                                                                                                                                            gATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGc                >  1:34747/1‑251 (MQ=255)                                                                                                                                                                                                                                  acacGTAAGTGATGCAGCGTGCGTAATCATTGGTCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCCCTGCGCCGCTACATTGTTCTTTGCCACTTTCATCTTTAACGCGGTATAGCACCGCCAgtt           >  2:420731/1‑248 (MQ=255)                                                                                                                                                                                                                                  acacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATa          <  1:320941/251‑1 (MQ=255)                                                                                                                                                                                                                                   cacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATa          >  1:404080/1‑250 (MQ=255)                                                                                                                                                                                                                                   cacGGAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGCGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATa          >  1:243076/1‑250 (MQ=255)                                                                                                                                                                                                                                           tGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCATGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGa  >  2:390738/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAA‑TGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGA  >  NC_000913/4373178‑4373660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑