Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,337,864	C→T	V22V (GTG→GTA)	adiY ←	DNA‑binding transcriptional activator AdiY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,337,864	0	C	T	100.0%	110.8 / NA	33	V22V (GTG→GTA)	adiY	DNA‑binding transcriptional activator AdiY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/21);  total (12/21)

CGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTCACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGG  >  NC_000913/4337624‑4338096                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         cgcgGCAACGTGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTTCGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATATCGCCATATGGTTAGCTTTAAGGCTAATAGGATCTTTCCCATTTACCCTTATcc                                                                                                                                                                                                                                <  2:533393/251‑1 (MQ=255)     gCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGAc                                                                                                                                                                                                                            <  2:215393/251‑1 (MQ=255)        aCGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGCTAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATc                                                                                                                                                                                                                         >  1:383801/1‑251 (MQ=255)                          gtcgggCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAAc                                                                                                                                                                                                       <  1:468784/247‑1 (MQ=255)                                      aGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTg                                                                                                                                                                                           >  2:157824/1‑251 (MQ=255)                                                       aGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTGGGTCGCTGCAAATCCTc                                                                                                                                                                           <  1:186170/250‑1 (MQ=255)                                                        gcgcTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCAt                                                                                                                                                                         <  1:239420/251‑1 (MQ=255)                                                                               ttGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAAt                                                                                                                                                   <  1:516095/250‑1 (MQ=255)                                                                                    agagaTCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGAGCGAATaaa                                                                                                                                                >  2:281582/1‑248 (MQ=255)                                                                                                         ggAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTaaagcaaag                                                                                                                        <  2:44165/251‑1 (MQ=255)                                                                                                                       atgatgTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAAt                                                                                                          >  1:417499/1‑251 (MQ=255)                                                                                                                       atgatgTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAAt                                                                                                          <  1:433604/251‑1 (MQ=255)                                                                                                                                     aaTATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGt                                                                                            <  2:446092/251‑1 (MQ=255)                                                                                                                                     aaTATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAAGATTATTGTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCATACATCTTTTTCAGTCAATAAAACATTACAAGGTTGGTCGCTGCAAATCCTCATACATGTGTTCCTGAGTGCGAATAAAAGACCATTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTg                                                                                             <  1:562394/250‑1 (MQ=255)                                                                                                                                        atGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTat                                                                                          <  2:323922/250‑1 (MQ=255)                                                                                                                                          gAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTagga                                                                                        <  2:171567/250‑4 (MQ=255)                                                                                                                                           aGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAAt                                                                                                                                                                               >  1:77005/1‑162 (MQ=255)                                                                                                                                           aGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAAt                                                                                                                                                                               <  2:77005/162‑1 (MQ=255)                                                                                                                                                                agagGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAAt                                                                 <  1:426522/251‑1 (MQ=255)                                                                                                                                                                   ggagACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCACTAAAACAATACACGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAAc                                                              >  1:376785/1‑251 (MQ=255)                                                                                                                                                                   ggagACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGGACTCCTGAGTGCGAATAAAAGACCAGTTCGCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTAGGATGAAGATAAACATGCATAAAAATAAc                                                              <  2:341409/251‑1 (MQ=255)                                                                                                                                                                   ggagAAGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTGATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCATGCAATAAAACAATACAAGGTTTGTCGCTGCAAATCCTCATACATTTACTCCTGAGTGCGAATAAAAGATCAGTTCTCGCTAAAGCAAAGCGATTCCGGGAAATGGTTATATAATTGTATGATGAATATAAACATCCATAAAAATAAc                                                              <  2:266462/251‑1 (MQ=255)                                                                                                                                                                    tatttttatgcatgtttattTTCGCAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTATTTCCAATTTACCATTATCAAGACATCTTTTTCAGTCAATAAAACAATATAAGGTTGGTCGCTGCACATCTTCATACATGTACTCCTGAGTGCGAATAAAAGAGCAGATCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAAAATGAATAAAAATa                                                                >  2:166311/16‑248 (MQ=255)                                                                                                                                                                    tatttttatgcatgtttattTTCACAATTTAATAACTCCCTATGGTTAGCGTTAATGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGTTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATa                                                                <  1:166311/233‑1 (MQ=255)                                                                                                                                                                    gagaCGTCGATAATATTATTTTCACAATTTAATAATGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATACAACAATACAAGTTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATACTTGTATGATGAATATAAACATGCATAAAAATAACg                                                             >  2:439100/1‑251 (MQ=255)                                                                                                                                                                      gaCGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAg                                                           <  1:184988/251‑1 (MQ=255)                                                                                                                                                                           agATAATATTATTTTCACAAATTAATAACGCCATATGGTTAGCTCTAAGGCTAATAGGTTCTGTACCATTTAACCTTATCCAGACATCTGTTTCTGTCAATAAAACAATATAAGGTTGGTCGCTGCAAATCCTCATACATGTAATCCTGAGTGCGAATAAAAGACAAGTTCTCGGTGAAGCAAAGCGATACCGGTAAATGGTTATATAATTGTATGGTGAATATAAACATGCATAAAAATAACGAGTTTTa                                                      <  2:99386/250‑1 (MQ=255)                                                                                                                                                                             ataataTTATTTTCACAATTTAATCACGCCATATGGTTAGCTTTAAGTCTAATAGCTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAACGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACt                                                    >  2:197283/1‑251 (MQ=255)                                                                                                                                                                                    tattTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGt                                              >  1:432822/1‑250 (MQ=255)                                                                                                                                                                                                                         aaGGCTAATAGGTTCTTTCCCATGTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGATGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATAtttttt        >  1:255521/1‑251 (MQ=255)                                                                                                                                                                                                                          aGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATAttttttt       <  1:382909/251‑1 (MQ=255)                                                                                                                                                                                                                               aaTAGGTTCTGTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACGCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACa    <  1:265338/249‑1 (MQ=255)                                                                                                                                                                                                                                aTAGGTTCTTTCCCATTTACACTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAgt  >  1:407854/1‑249 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         CGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTCACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGG  >  NC_000913/4337624‑4338096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑