Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,301,071	C→T	A670T (GCA→ACA)	mdtO ←	putative multidrug efflux pump subunit MdtO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,301,071	0	C	T	100.0%	162.2 / NA	54	A670T (GCA→ACA)	mdtO	putative multidrug efflux pump subunit MdtO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/25);  total (29/25)

CTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCATCCC  >  NC_000913/4300827‑4301307                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             cTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGc                                                                                                                                                                                                                                        >  1:205616/1‑251 (MQ=255)         cATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGt                                                                                                                                                                                                                                >  1:586197/1‑251 (MQ=255)             gAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTgg                                                                                                                                                                                                                            >  1:8606/1‑251 (MQ=255)                ttgttTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCAAAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCa                                                                                                                                                                                                                          >  1:65487/1‑250 (MQ=255)                  gttTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGc                                                                                                                                                                                                                       <  2:152901/251‑1 (MQ=255)                                 ccTGCGGCCAGCCGGAGCTGGCACGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCGGTCCCGTCTGGCAAGAGGGCGAAAAGCTGGc                                                                                                                                                                                                        >  2:286286/1‑251 (MQ=255)                                    gCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCa                                                                                                                                                                                                       <  1:235677/249‑1 (MQ=255)                                     cGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGAc                                                                                                                                                                                                    <  1:154976/251‑1 (MQ=255)                                        ccAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAAGCCTTACGGACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGGTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACgtg                                                                                                                                                                                                 <  2:515154/251‑1 (MQ=255)                                            ccGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGGTTGGCAACCGGGCGGAAAGGTGGCAGACGGGTTgg                                                                                                                                                                                             >  2:125988/1‑250 (MQ=255)                                             cGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCt                                                                                                                                                                                            <  1:71432/251‑1 (MQ=255)                                             cGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGACCGGCAAGCggggggaacgctgggaggcggggtggc                                                                                                                                                                                            >  2:182596/1‑227 (MQ=255)                                                aGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGCAAAGCTGGCAGCCGTGTTGCTCCt                                                                                                                                                                                         >  1:301900/1‑251 (MQ=255)                                                    ggCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAAGCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCGGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGt                                                                                                                                                                                       <  1:38264/249‑1 (MQ=255)                                                       aaGATGAATAGCGGCGGGCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCATTCGGGCAAGCGGGCGAAAAGCGTGCAGACGTGTTGCTCCTGTTTtaa                                                                                                                                                                                  <  2:579457/251‑1 (MQ=255)                                                         gATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGGGTTGCTCCTGTTTtaat                                                                                                                                                                                 >  1:134732/1‑250 (MQ=255)                                                           tGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCCGTCTTGCAAGCGGGCGCAAATCTGGAAGCCGTGTTGCTCATGtttttaca                                                                                                                                                                                >  2:503735/1‑245 (MQ=255)                                                           tGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGGTAATGTCGGGGCTTTCCCGACTGGCAAGCGGGCGCAAAGCTGGCAGACGTGTTGCTCCTGTTTtaata                                                                                                                                                                                >  2:198005/1‑249 (MQ=255)                                                            gAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTCATAAgg                                                                                                                                                                             >  1:177650/1‑251 (MQ=255)                                                                      ggCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGATGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGAt                                                                                                                                                                   <  1:201436/251‑1 (MQ=255)                                                                      ggCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGAt                                                                                                                                                                   <  1:182596/251‑1 (MQ=255)                                                                       gCCAGTTTGATTTGCTCCGGTTTGAGCTGCTGAAGAGGTGCCGAATCCTCACGCACCAGGGCCCAGCCGGACATCAGCGTCGTGCTGCCGAGAATGCTGCACACCCGCAGACGTGAAAGTTGAAGATTGCTCATTGCCTGGCTCCTTGTGACTGTTCAGTGGCGGGCGTTACTGTCTGGGCTGTCCACTTCGGCCAGCGGGCGCAAAGCTAGCCGACGTGTTGCTCCTGTTTTTATATGGTGGGCAggtac                                                                                                                                                                  >  2:530731/1‑248 (MQ=255)                                                                        ccAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTTCAGTCTGGCAAGCGGGCGGAAAGCTGGCAGACGTGTTTCTCCTGTTTTAATAAGGTGGGCAGGATgg                                                                                                                                                                 >  2:284477/1‑251 (MQ=255)                                                                           gTTTGATTTGTTCCGGTTTGAGCTGGTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCTGGGCTGTCCAAACTGGCAAGCGGGCGGAAAGCGGGCAGACGTGTTGCTCCCATTTTAATAAGGTGGGCAggtaggcct                                                                                                                                                              >  2:492148/1‑244 (MQ=255)                                                                                   tGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGc                                                                                                                                                       <  1:284602/250‑1 (MQ=255)                                                                                      tCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCCGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCgggg                                                                                                                                                   >  1:112601/1‑250 (MQ=255)                                                                                       ccGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGGAAAGCTGGCAGACGTGTTGCTCCTGGTTTAATTAGGGGGGCAGGATGGCCTGAGAGGCGGgtg                                                                                                                                                  >  2:431961/1‑251 (MQ=255)                                                                                              gAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTc                                                                                                                                           <  1:326719/251‑1 (MQ=255)                                                                                               aGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAACGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGGGGGCAGGATGGCCCgaggggagggggggtcgtct                                                                                                                                          >  2:224463/1‑234 (MQ=255)                                                                                                   gTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCCCCTGTTTTTATAAGGGGGGCAGGATGGCCTggggggggggtgggttttttagcg                                                                                                                                      >  2:238841/1‑228 (MQ=255)                                                                                                    ttGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCCGTCTGGGAAGCGGGGGAAAAGCTGGCAGCCGGGTTGCTCCTGTTTTAATAAGGGAGGCAGGAAGGCATGAGGGGCGGGTGTTTCttttatcgg                                                                                                                                     >  2:478670/1‑244 (MQ=255)                                                                                                     tgatgaGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGGTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCggggggtcttttcatgggt                                                                                                                                    >  2:324858/1‑235 (MQ=255)                                                                                                      gatgaGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCGCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGtt                                                                                                                                    <  1:249980/250‑1 (MQ=255)                                                                                                       ctgaGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGCTTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCCGGCTTGCAAGCGGGCGAAAAGCTGGCAGACGGGTTGCTCCTGTTTTAATAAGGTGGGCAGGAGGGCCTGAGAGGCGGGGGGCTCTTctaacggttt                                                                                                                                  >  2:113597/2‑242 (MQ=255)                                                                                                                  aaTCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAAc                                                                                                                       <  1:406672/251‑1 (MQ=255)                                                                                                                   aTCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCCGACGTGTTGCTCCTGTTTTAATAAGGGGGGCAGGATGGCCTGAGAGGCGGGGGTGTCTTCAAGAGTTTTTTTAGGGGAACg                                                                                                                      >  1:326781/1‑251 (MQ=255)                                                                                                                   aTCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGGGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGGCTCtttaaagggtattttgagggga                                                                                                                        >  1:153287/1‑229 (MQ=255)                                                                                                                       ttACGTACGAGGGCACAGCCGGAACACAACGTCGTGCTGCCGAGAATGCTGCACAACAGCAGCCGTGAAAGTTGACGATTGATCATTGCGTGGCCCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCCGCCCAGTCTGGCAAGCGGGCGAAAAGGTGGCAGCCGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCt                                                                                                                   <  1:783/250‑1 (MQ=255)                                                                                                                        tACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTg                                                                                                                  <  2:326852/250‑1 (MQ=255)                                                                                                                          cGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTTCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTTACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGTCTGAGt                                                                                                               <  1:446521/251‑1 (MQ=255)                                                                                                                                aGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGACCAGTCTGGCAAGCGGGCGCAAAGCTGGCAGACGTGTTGCTCCTGTTTTACTAAGGCGGGCAGGAAGGCCTGAGAGGCGGGGGTTTTTTCTCGCGTTAtttttggcgacaggcggagggcggtt                                                                                                         >  1:253878/1‑228 (MQ=255)                                                                                                                                     acaGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGTGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAg                                                                                                    <  2:35743/251‑1 (MQ=255)                                                                                                                                        gCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGAcc                                                                                                 <  1:456091/251‑1 (MQ=255)                                                                                                                                         ccGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCCGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCGCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCg                                                                                                <  1:44758/251‑1 (MQ=255)                                                                                                                                              aaTCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGc                                                                                           <  1:198005/251‑1 (MQ=255)                                                                                                                                                             gcCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGATTGCGGTTGCCAGACCGGCAGCGTATTTCACCAGGGc                                                                            <  2:348432/251‑1 (MQ=255)                                                                                                                                                             gcCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCAGTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGc                                                                            <  2:134732/251‑1 (MQ=255)                                                                                                                                                                 agaATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCt                                                                                                                                                                                         <  2:48015/138‑1 (MQ=255)                                                                                                                                                                 agaATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCt                                                                                                                                                                                         >  1:48015/1‑138 (MQ=255)                                                                                                                                                                                agcagACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTTAAGGGTTATTTTAGGAGAAAGGCTGGGGGGGGGTGCCAGACCGGCAGCGGATTTTTCCCGGGCGGCGGCCAACTGAACTGct                                                         >  1:11136/1‑251 (MQ=255)                                                                                                                                                                                                  cGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGTGCGAAAAGCTGTCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATGTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCg                                       <  1:210658/251‑1 (MQ=255)                                                                                                                                                                                                   gATTGATCATTGCGGGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCCGACGTGTTGCTCCTGTTTTAATAAGGTGGGCCGGATGGCCTGAGAGGCGGGGGTGTCTTCCAGCGTTTTTTTAGGAGAACGGCCTAGGGCGGTGTCCCCACCCGGAGCGTCTTTTTCCCCGGCGTCGGCACACCGAACTCCCCTGTCCGGCTGTATTGCGt                                      >  1:318353/1‑251 (MQ=255)                                                                                                                                                                                                           aTTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAgc                              <  1:286286/251‑1 (MQ=255)                                                                                                                                                                                                                                       ggcgggcgTTAATGTTGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGGGTGCGGTTGCCAAACCGGCAGCCGATTTCTCCAGGGCGGCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCCGCGCCTGCGCCGCGGTCCCGGTGGCATccc  >  1:444847/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             CTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCATCCC  >  NC_000913/4300827‑4301307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑