Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,137,212	C→T	R313R (CGC→CGT)	flgI →	flagellar P‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,137,212	0	C	T	100.0%	157.1 / NA	50	R313R (CGC→CGT)	flgI	flagellar P‑ring protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/35);  total (15/35)

CCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTAAAGGCGAAAGCGGGCGAAGATCC  >  NC_000913/1136978‑1137456                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        ccTTGCCGATATTCAGAATATGCAGGTTAATGTCGCCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCg                                                                                                                                                                                                                                          <  2:21188/250‑1 (MQ=255) ccTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCGCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCg                                                                                                                                                                                                                                          <  1:487182/250‑1 (MQ=255)                 aaTATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTcccttcgaagcggtcgttt                                                                                                                                                                                                                         >  1:370418/1‑233 (MQ=255)                    aggcagtttaagtgcACCCCGCAGGACGCGAAAGGAGTGATTAGCTCGCGCATCGGTTCGGTGGTGAGGAATATCGAAGTGACCCTCGACAGCTGCGCGGGAGCGCAGGGGAATCTCACAGTAACATTTAATCGTCAGGACAATTTCATCCAGCCAGATAGACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGTAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAg                                                                                                                                                                                                                     <  2:466173/237‑1 (MQ=255)                         ggTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCa                                                                                                                                                                                                                <  1:159372/251‑1 (MQ=255)                               tGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTTGCT‑TCAAAGAGTACGTTTCAgccccccccccc                                                                                                                                                                                                          >  1:492051/1‑241 (MQ=255)                                   aCCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGGTCGCT‑GCAAAACGTACGtttccgcccccgccaccata                                                                                                                                                                                                       >  2:398381/1‑232 (MQ=255)                                     cccGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGAGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTACTCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTCCGTCAGAGCGGCGGATCGCTCGCAAAG‑GTACGGTCCAACGCCAGCCCCaaaaaac                                                                                                                                                                                                    >  1:556548/1‑246 (MQ=255)                                         cAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACgtggt                                                                                                                                                                                                <  1:469770/251‑1 (MQ=255)                                          aGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACgtggtg                                                                                                                                                                                               <  2:167855/251‑1 (MQ=255)                                                       agtGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGTCAGCCTCAATAACGTGGTGCGCGCGCTCAAt                                                                                                                                                                                   <  1:512522/250‑1 (MQ=255)                                                                                          aTCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCGGATGTCCATa                                                                                                                                              <  2:397739/251‑1 (MQ=255)                                                                                            cgcgAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GGAAAAAGGACGTTCCAAGGCCAGCCTCCATAACGGGGGGCGCGCGCTCAATGCGC‑CGGGCGCTACGCCGGTGGATcagaaggggaggg                                                                                                                                             >  1:526327/1‑238 (MQ=255)                                                                                            cgcgAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAAGCGTCAGGCCAAGGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCGCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATc                                                                                                                                                         <  2:526327/238‑1 (MQ=255)                                                                                                           gACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTATACGTCAGGCCAATGTCAGCCAGCCAGATACACCGGTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGGACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAg                                                                                                                            <  2:497025/251‑1 (MQ=255)                                                                                                           gACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAg                                                                                                                            <  2:307519/251‑1 (MQ=255)                                                                                                            aCAGCTGCGCGGTCGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTGGCT‑GCCAAGCGTAAGTTCCCGCGCCCGCCTCACAAAAGTGGGGCGCGCGGGCAATGCGC‑CGGGCGCTCCGCCGATGGGGCTGATGTCCAACCTGcgatc‑aattgaaagg                                                                                                                           >  1:397599/1‑244 (MQ=255)                                                                                                            aCAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTAATCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTAGCT‑GCAAAGCGTAAGTTCCAGCGCCAGCCTCAATAACGTGGTGCGAGCGCTCAAAGCGC‑TGGGGGCTCAGCCGATGGAACTGGTATCCATACTGCAATC‑AATGCAAAgg                                                                                                                           >  1:208154/1‑250 (MQ=255)                                                                                                               gCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCg                                                                                                                        <  2:557788/251‑1 (MQ=255)                                                                                                                 tGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTTGCT‑GCAAAACGTAAGTTCCCGCGCCCGCCCCCATAAAGTGGTGCGCGCGCTCCATGCGC‑TGGGCGCTACGCCGCTGGGTCTGGTGTCCATACTGCAATC‑AATGCaaaaggcggg                                                                                                                      >  1:180035/1‑244 (MQ=255)                                                                                                                  gcgcGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCggg                                                                                                                      <  1:454583/250‑1 (MQ=255)                                                                                                                  gcgcGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGa                                                                                                                     <  2:492051/251‑1 (MQ=255)                                                                                                                   cgcgGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCCAAACGTACGTTCCACCGCCCGCCCCCATAAAGTGGTGGGCGCGCCCCATGCGCCGGGGCGCT‑CGCCGGTGGGTCTGTTTTCCCTACTGGAATCCAATCCAAA‑TGCggggg                                                                                                                    >  2:458772/1‑249 (MQ=255)                                                                                                                      gggaGCGCAGGGGAATCTCTCAGTAACAGTTAAGCGTCAGGCCAATTGCAGCCAGCCAGATACACCGTTTTGTGGTGGACAGTCTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCTGTTCGCT‑GCAAAGCGTACGTTCCATCGCCAGCCGCAATTACGTGTTGCGCGCGCGCAATGCGC‑TGGGCGCTACGCCGATGGAGCTGTTGTCCATACTGCAATC‑AATGCAAATGGCTGGATGTc                                                                                                                 <  2:73382/248‑1 (MQ=255)                                                                                                                      ggTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTc                                                                                                                 <  2:464/251‑1 (MQ=255)                                                                                                                             cAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGc                                                                                                          <  1:507484/251‑1 (MQ=255)                                                                                                                                  gaacctctcAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGGCTGCGGGCAAAAc                                                                                                     <  2:523037/247‑1 (MQ=255)                                                                                                                                        cgcAGTAACAGTTAATCGTCAGGTCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGAAAAGTGCGGGATGTCTGCGGGCAAAACTGGaaa                                                                                               <  1:332204/249‑1 (MQ=255)                                                                                                                                            ggaccaggtAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTACATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAatcat                                                                                           <  2:441868/243‑1 (MQ=255)                                                                                                                                                        cGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCC‑GCAAAAAGTACGTTCCACCGCCCACCCCAAAAAAGTGGGGCGCGCGCTCAAAGCGG‑TGGGCGCTCCGCCGATGGATCTGATGGGCATACTGCAATC‑AAAGCAAAGAGCGGGGTGGCTGGGGGGGAAACTGGGAAACATCTGATGATCatg                                                                               >  2:445305/1‑249 (MQ=255)                                                                                                                                                         gTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAACGCACGGTCCAACGCCAGCCTCAAAAAAGTGGGGGGAGCGCCCAATTCGC‑TGGGCGGTACCCCGATGGGGCTGGTGTCCCTACTGCAATC‑AATGCAAAGAGCGGGGTGTCTGCGGGCAAAAATGGGAATCATCTGATAATCAGCg                                                                              >  1:165925/1‑251 (MQ=255)                                                                                                                                                              gCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGc                                                                         <  1:290226/251‑1 (MQ=255)                                                                                                                                                                     tCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAACGGACGTTTCAGCGCCAGCCTCAATAACGTGGGGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGGTGGATTTGATGTCCATACTGCCATC‑AATGCAAAGTGCGGGGTGTCTGCGGGCAAAACTGGGAATCATCTGATGATCAGCGACAGCAAaatac                                                                  >  1:440323/1‑251 (MQ=255)                                                                                                                                                                         ccagccagATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGc                                                              <  1:336328/251‑1 (MQ=255)                                                                                                                                                                                   acacCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGcc                                                    <  1:445305/251‑1 (MQ=255)                                                                                                                                                                                     acCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTg                                                  <  2:180035/251‑1 (MQ=255)                                                                                                                                                                                       cGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTggg                                                <  1:405052/251‑1 (MQ=255)                                                                                                                                                                                       cGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAACGTACGTTCAAGCGCCAGACCCCATAAAGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGCTGGGTCTGATGTCCCTAATGCAATC‑AAAGCAAAGGGGGGGGTGTCTGCGGGCCAAACAGGAAATCATCTGATGATCAGCGACCACAAAACACTGGCAAGTGCGGCCCggg                                                >  1:477688/1‑251 (MQ=255)                                                                                                                                                                                                gtggACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGGGGACGTTCCAGCGCCAGCCTCCAAAACGTGGGGCGCGCGCTCAATGCCC‑TGGGCGCCACGCCGGTGGGTCTGATGTCCCTACTGCCATC‑AATGCAACGAGCGGGGTGTTTGCGGGCAAAACCGGAAATCCTCTGATGGTTAGCGACAGGAAACCACTGGCAAGTGCGGCCCGGGATGGGCAAt                                       >  1:287411/1‑251 (MQ=255)                                                                                                                                                                                                          tgtgGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACg                              >  1:441172/1‑250 (MQ=255)                                                                                                                                                                                                          ggtgGTTACGCCACAAACGCAGACCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCCCTCAACGa                             <  2:219627/250‑1 (MQ=255)                                                                                                                                                                                                           gtgGTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGAGCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGaa                            <  2:57619/251‑1 (MQ=255)                                                                                                                                                                                                              gTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTa                         <  1:483298/251‑1 (MQ=255)                                                                                                                                                                                                              gTTACTCCACAAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTa                         <  1:506329/251‑1 (MQ=255)                                                                                                                                                                                                                       caAACGCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAAGCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTAAAGGCGaaa                <  2:473725/251‑1 (MQ=255)                                                                                                                                                                                                                            gCAGATCGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGGTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTAAAGGCGAAAGCggg           <  2:437045/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCTACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTAAAGGCGAAAGCGGGCGAAg      <  1:266660/250‑1 (MQ=255)                                                                                                                                                                                                                                  cGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTAAAGGCGAAAGCGGGCGAAg      <  2:484378/250‑1 (MQ=255)                                                                                                                                                                                                                                  cGATTTACGTCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTAAAGGCGAAAGCGGGCGAAGa     <  1:458772/251‑1 (MQ=255)                                                                                                                                                                                                                                      ttACGTCAGAGCGTCGGGTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGGGCGCTCAATGCGC‑TGGGCGCTACGGCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGAGGCGCAATCACTCAACGAACTAAAGGGGAAAGCGGGCGAAGagcc  <  2:507784/250‑4 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        CCTTGCCGATATTCAGAATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGTTCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTCTCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCT‑GCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGC‑TGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATC‑AATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTGGGATGCGCAATCACTCAACGAACTAAAGGCGAAAGCGGGCGAAGATCC  >  NC_000913/1136978‑1137456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑