| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,249,684 | C→T | A44V (GCT→GTT) | malM → | maltose regulon periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,249,684 | 0 | C | T | 98.4% | 201.6 / NA | 62 | A44V (GCT→GTT) | malM | maltose regulon periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (34/27); minor base G (0/1); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGCTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTCCCC > NC_000913/4249438‑4249928 | cATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTgttgcg < 1:438142/251‑1 (MQ=255) ggCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGca < 2:447735/251‑1 (MQ=255) cATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGcaac > 2:475279/1‑251 (MQ=255) aTAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGcaac > 1:503009/1‑250 (MQ=255) aaCAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAAGAAAAGACTCATCGTCCTCTGTTTAGCAGCAGGGTTACTGGCAAGCGCGCCTTGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCa < 1:360252/251‑1 (MQ=255) aCAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTGGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATGAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCAc < 2:60861/251‑1 (MQ=255) cAGGTTGTCATTATCTGAAAGGGGCTAAAGCCCCTCTGATTAGCGGTTTTAGCGCGCTATAGCCTGGCTACCGCTGAGCTCCAGATTTTTAGGTGAAAACAATGAAAATGAATAAAATGCTCATCGTCCTCTGTTTATCAGCATGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCTCAAAACTCCAGCGACGCGCCAGCCATTCCATCTGTGGCTCTGcaacatttctcc < 2:356398/251‑8 (MQ=255) gTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTgg > 1:159544/1‑251 (MQ=255) cATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGTGTTACTGTCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCTCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGacac < 1:92832/250‑1 (MQ=255) gAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGTTGCAACAACTCACCTGGACACCGGTCGATc > 2:98379/1‑251 (MQ=255) gAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCg < 1:466567/248‑1 (MQ=255) cccTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAAccc > 1:184290/1‑248 (MQ=255) aTCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCGTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCGAAAACCCAGACCACCCAACt < 2:345908/251‑1 (MQ=255) tAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACcg > 1:440787/1‑251 (MQ=255) ttGCCTGGCTAACGCTGAGCTCAAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAACCCCAGACCACCCAACTGGCGACCGGCGGCcaac > 2:54967/1‑250 (MQ=255) tGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCcaaca > 1:310163/1‑250 (MQ=255) gggCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGa < 2:321778/250‑1 (MQ=255) ggCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTg < 1:475279/249‑1 (MQ=255) tACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGt > 1:281657/1‑251 (MQ=255) cAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCCGACCACCCAACTg > 2:488546/1‑212 (MQ=255) cAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGGCGATCAATCTAAAACCCAGACCACCCAACTg < 1:488546/212‑1 (MQ=255) ttGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTcc < 1:424533/250‑1 (MQ=255) gTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGGCCGGTtgc > 1:108891/1‑251 (MQ=255) aaaaCAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTtgctgc > 1:329530/1‑251 (MQ=255) aCAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCCGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCATTATAAAACCCAGACCACCCAACTGGCACCCGGGGCCCAACAACTGAACGTTCCCGGCATAAGTGGTCCGGTTGCGGCGtc > 1:207021/1‑250 (MQ=255) aCAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCgg > 1:228692/1‑163 (MQ=255) aCAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCgg < 2:228692/163‑1 (MQ=255) aCAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGt > 2:216314/1‑250 (MQ=255) aatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTcc < 1:216314/251‑1 (MQ=255) aatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTcc > 1:156479/1‑251 (MQ=255) tgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCCTCAGTGGTCCGGTTGCTGCGTACAGCGTCCCg > 1:407278/1‑251 (MQ=255) aTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGc > 2:4779/1‑250 (MQ=255) aaaaGTCTCATCGTCCTCTGTTTATCAGCAGGGTTTCTTGCCAGCGCGCCTGGAATTAGCCTTGCCAATGTTAACTCCGTACCGCAAAAAACCAGCGTCGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCTCCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTTGTCCGGTGGGTGCGTACAGCTTCCCGGCaaa < 2:394157/251‑1 (MQ=255) aaaaGTCTCAGCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCaa < 2:73025/250‑1 (MQ=255) aaaGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTAGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAAc > 1:142388/1‑251 (MQ=255) aaGTCTCATCGTCCTCTGGTTAGCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGGGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACa < 2:281657/251‑1 (MQ=255) aGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGACTGGAATTAGCCTTGCCGATGTTAAATACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCACTGCAACAAATAACCTGGACACCGGTCGATCAATCTAACACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCCGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACAt > 1:585892/1‑251 (MQ=255) tcATCGTCCTCTGATTCTCAGCAGGGTTACTGGCAAGCGCGCATGGAATTAGCATTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCACTCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGACAACAACTGAACGTTCCCGGCATCAGTGGTCCGATTGCTGCGTACAGCGTCCCGGAAAACATTgcc > 1:185415/1‑249 (MQ=255) caacGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCTCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCGAAAGCCCAGACCACCCAACTGGCTACCGGCGGCCAACAACTGAGCGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTg < 2:279619/245‑1 (MQ=255) ctctGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGAc > 2:126007/1‑251 (MQ=255) gTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGAcc < 2:421513/248‑1 (MQ=255) tttATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTg > 1:519749/1‑250 (MQ=255) tttATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGAGTCCCGGCAAACATTGGCGAACTGACCCTgc > 1:318541/1‑250 (MQ=255) ggTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGc < 1:4779/251‑1 (MQ=255) aCTGGTAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGGACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGGTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGCCCACCCAACTGGCGGCCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGaa < 2:322265/250‑1 (MQ=255) aCTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGaa < 1:98379/250‑1 (MQ=255) cTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGAACAGCGAAgg > 2:250787/1‑250 (MQ=255) tGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTACCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTg > 1:405983/1‑251 (MQ=255) ggCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGa < 1:20829/251‑1 (MQ=255) aaGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGaac > 1:466994/1‑250 (MQ=255) aaGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGaac > 1:462664/1‑250 (MQ=255) tGCCGATGTTAACTACGTACCGCAAAACACCAGTGACGCGCCAGCCATTCCAACTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTgc < 2:421487/249‑1 (MQ=255) ccGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAATCCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTTACCCTGACGCTGACCAGCGAAGGGAACAAACAAACCAGCGTTTTTGCGCCg < 2:14684/251‑1 (MQ=255) aTGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTTGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAAc < 1:238390/251‑1 (MQ=255) aTGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAAc > 2:167139/1‑251 (MQ=255) cTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCAACTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGAGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCCGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTgt > 2:238131/1‑250 (MQ=255) aaCACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGa > 2:162900/1‑250 (MQ=255) ccAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCCCCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATg < 2:306175/251‑1 (MQ=255) aGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGAc > 1:558924/1‑251 (MQ=255) gCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGTTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATgtcc < 1:517783/251‑4 (MQ=255) cGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACCCCGGTCGATCAATCTAACACCCAGCCCACACAACTTGCGACCGGCAGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGCTGCTGCGTACAGCGTCCCGGCAACCATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGACCAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGAccc > 1:263850/1‑251 (MQ=255) ccATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTcc < 1:437840/250‑1 (MQ=255) tCTGTTGCGCTGCAACAACTCACCTGGACACCGGACGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACACACCAGCGATTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTcccc > 2:185564/1‑249 (MQ=255) | CATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGCTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTCCCC > NC_000913/4249438‑4249928 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |