Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,638	T→C	intergenic (‑10/‑190)	iclR ← / → metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,638	0	T	C	100.0%	216.5 / NA	64	intergenic (‑10/‑190)	iclR/metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/44);  total (20/44)

CAGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTG  >  NC_000913/4223392‑4223884                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          cAGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCctttt                                                                                                                                                                                                                                                       <  1:385163/251‑1 (MQ=255)  aGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATGGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCcttttt                                                                                                                                                                                                                                                      <  2:159570/251‑1 (MQ=255)  aGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCAGCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCcttttt                                                                                                                                                                                                                                                      <  1:250179/251‑1 (MQ=255)   gTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTc                                                                                                                                                                                                                                                     <  2:444408/251‑1 (MQ=255)        ccAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGGTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTGAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTGCTGTAt                                                                                                                                                                                                                                                <  1:406119/251‑1 (MQ=255)            ccTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGGCCATGACAGTCTCCTTTTTCGGTAT‑CGTg                                                                                                                                                                                                                                           <  2:351995/251‑1 (MQ=255)               gACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTGTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGaa                                                                                                                                                                                                                                        <  2:552434/251‑1 (MQ=255)               gACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACGCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTGTTCTGTAT‑CGTGGaa                                                                                                                                                                                                                                        <  2:386558/251‑1 (MQ=255)                gcGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGGCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCTGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTTGaaa                                                                                                                                                                                                                                       <  2:388785/250‑1 (MQ=255)                     cGAAACCCTTCGGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATGCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGGCGCGGGTGCGGTGGCGACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTTCGACCATGACAGTCGCCTTTTTCTGTAT‑CGTGGAAAtcatt                                                                                                                                                                                                                                  <  2:245400/251‑1 (MQ=255)                       aaaCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGGTTTGCGGGGATGGGGGCGGCCATGACAGGCTCCCTTTTTTGTTT‑CGTGGGAAtcctttt                                                                                                                                                                                                                                >  1:62243/1‑251 (MQ=255)                              tgtgTTGCATCGTTGTTAGGAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTACGTGAGTGCCACACTGCCATTTGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAAtcattttcattt                                                                                                                                                                                                                           <  2:416411/247‑1 (MQ=255)                               ctgTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTTCCGCGGTTTGCGGGGATGGGTGGGGCCCTGACAGGCTCCTTTTTCTGGAT‑TGTGGGAAtcatttttctttttt                                                                                                                                                                                                                        >  1:191843/1‑243 (MQ=255)                                        cGTGGTTAGCAGGCGGTTGGTCGTGGAATTGGGTAACCCGTCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACGCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGc                                                                                                                                                                                                                <  1:525868/250‑1 (MQ=255)                                           ggTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAAt                                                                                                                                                                                                            <  1:112190/251‑1 (MQ=255)                                              tAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGc                                                                                                                                                                                                          <  2:211888/250‑1 (MQ=255)                                              tAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCCCGGTTCGCGGGGATGGGGGGGGCCATGACAGGCTCCTTTTTCTGTTT‑CGTGGGAATAATTTTCATTTTTTTTTTTAGCTAATGc                                                                                                                                                                                                          >  1:581189/1‑250 (MQ=255)                                                   ggCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCGAATGCAATAGt                                                                                                                                                                                                    <  2:421381/251‑1 (MQ=255)                                                     cGGTGGGTCGTGGAATTGGGGAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGATAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATGTTCATTTATATTGTTAGCTAATGCAATAGTTa                                                                                                                                                                                                  <  2:417242/251‑1 (MQ=255)                                                         gggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATGTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga                                                                                                                                                                                              <  2:158758/251‑1 (MQ=255)                                                                  aagtGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTGTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCg                                                                                                                                                                                     <  1:274082/248‑1 (MQ=255)                                                                       ggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGGGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCCCGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTTTCCCGTTTCGCGGGGGGGGGGGGGGCCATGAAAGTCTCCTTTTTTTGTAT‑CGTGGAAATACTTTTCTTTTTTATTTTTAGCTAATGAAATAGTTACTGACCTGATCCggtt                                                                                                                                                                                  >  1:370390/1‑246 (MQ=255)                                                                                         gcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTGTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAAtct                                                                                                                                                             <  2:97708/251‑1 (MQ=255)                                                                                              ttCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTCAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCCGTTCGCGGGGAAGGGGGGGACCCTGGCAGGCTCCTTTTTTTTTATTGGTGAAAAT‑ATTTTTATTTTTTTTGTTAGCTAATTCAATAATTACTGTACTGATCCGTTGGGTT‑AATATTTAAAAAAACTCAttt                                                                                                                                                        >  2:170369/1‑249 (MQ=255)                                                                                                ccGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTg                                                                                                                                                      <  1:468173/251‑1 (MQ=255)                                                                                                 cGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTTCCGCGTTTCTCGGGAATGGGTGCGACCATGACAGTCTCCTTGTTCTGTAT‑CGTTGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAGATTTACTGAACTGATCCGATGAGTT‑AATGTTGATCAAATCTCATGTTGc                                                                                                                                                     <  1:491926/251‑1 (MQ=255)                                                                                                          cacaCTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGTTTTGCGGGGATGGGTGCGACCCTGACAGTCTCCTTTTTTTGTAT‑TGTGGGAATCATTTTTATTTTTTTTTTTAGCTAATGCAATAGTTATTGAAATGATCCGATTGGTT‑TATGTTGAAAAAAtcttattgtgcggggtgggt                                                                                                                                            >  1:173961/1‑234 (MQ=255)                                                                                                           acacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGGATGGGGGCGACCCTGACCGTCTCCTTTTTTTGTAT‑CGTGGAAATCATTTTCATTTTTTTTTTTATCCTATGCCATATTTACTGAACTGATTCGATGGGTT‑TATTTTGTACAAATCTTCTGTTGCGTGGGGGTCg                                                                                                                                           >  1:384199/1‑251 (MQ=255)                                                                                                           acacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCg                                                                                                                                           <  2:148752/251‑1 (MQ=255)                                                                                                                     tGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTTCCGCGTTTCGCGGGGATGGGTGCGGCCATGACAGGCTCCTTTTTTTTTAT‑CGGGGGAATCATTTTTCTTTTTATTGTTTGATAATGCAATAGTTTCTGAACTTGTCCGGTTAGTT‑TATGTTGAACAAAtatcc                                                                                                                                                           >  1:153628/1‑221 (MQ=255)                                                                                                                     tGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACca                                                                                                                                  <  1:569603/250‑1 (MQ=255)                                                                                                                     tGGATTCGGCAATCCACTCCAGGAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCa                                                                                                                                                           <  2:153628/225‑1 (MQ=255)                                                                                                                       gATTCGGCAATCCACTCCAGTAAGTGCAGGCCGCGCGTTAATGACTGAACCTGTCCAGTCGGTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTGTCGCGGGAATGGGTGGGACCATGGCAGGCTCCTTTTACTGTAT‑CGTGGGAATCATTTTCATGTTTATTGTTAGCTAATGGAGTAGGTACTGAACTGATCCGATGATTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAg                                                                                                                              <  1:273937/251‑1 (MQ=255)                                                                                                                           cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGTTTCGCGGGGATGGGGGCGACCATGACAGTCTCCTTTTTCTTTTT‑CGTGGGAATCATTTTCATTTTTTTTTTTTGCTAATGCAATAGTTACTGAACTTATCCGCTGAGTT‑AATGTTTTACAAATCTCATGTTTCGGGGTGGGCGCTTTTTCCA‑CAGATGc                                                                                                                          >  1:318862/1‑251 (MQ=255)                                                                                                                                       ccAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGCTTTCTGCCGCGGTTCGCGGGAATGGGGGGCACCATGACAGTCTCCTTTTTTTGTAT‑CGTGGAAATAATTTTTATTTTTATTTTTTGCTAATGCCAAAATTTCTTAACTTGTACGTTGAGTTTAATTTTGA‑CAAATCTCATGTTGCCTGGGGGTCGGTTTTTCCAACAG‑TGCGGTTATGcccgt                                                                                                              >  1:31824/1‑248 (MQ=255)                                                                                                                                          gTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATg                                                                                                           <  1:528375/251‑1 (MQ=255)                                                                                                                                          gTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATg                                                                                                           <  2:507284/251‑1 (MQ=255)                                                                                                                                            aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCTGGTTTTCTGTCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGAGCCGATGAGTT‑AATGTTGAACAAATCTCATGGTGCGTGGGGGGCGCTTGTACCA‑CAGATGCGTTTATGCCAGTATg                                                                                                           <  2:585407/249‑1 (MQ=255)                                                                                                                                               ttCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGttt                                                                                                       <  1:449796/250‑1 (MQ=255)                                                                                                                                                    gCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGGTTTGCGGGGAGGGGGGGGACCCTGAAAGGCTCCTTTTTTTGTAT‑CGTGGGAAACATTTTCATTTTTTTTTTTTGCTAATGCAATAGTTTCTGAAACGATCCGATGGGGT‑TATGTTGAACAAATCTCTTTTTTGGTGGTGGTCGCTTTTACCA‑CAGAATCGTTTTTGcccgattgggttgttgg                                                                                                  >  2:436697/1‑235 (MQ=255)                                                                                                                                                       ccgcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAGCTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAAttt                                                                                              <  2:424486/250‑1 (MQ=255)                                                                                                                                                          cgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTCGCGGGAATGGGGGGGACCCTGACCGTCTCCTTTTTTTGTTT‑CGTGGGAATCATTTTCATTTTTTTTGTTAGTTAATGCAATTGTTACTGAACTGGTCCGGTGAGTT‑AATGTTGGACAAATCTCCTGTTGCGTGGTGGTCGCTTTTTCCA‑CAGATGCGTTTTTGCCAGTATGGTTTTTTGAAtttttt                                                                                           >  2:385649/1‑250 (MQ=255)                                                                                                                                                                 gACTGAACCTGTCCAGTCGCTGGGGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGGGCGACCATGACAGTCTCCTTTTTCTGTAG‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACGGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTTGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGGTGAATTGTTATTAAATc                                                                                    <  2:257737/251‑1 (MQ=255)                                                                                                                                                                  aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGTGAATGGGTGCGACCATGACAGTCGCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTGGCGTGGTGGTCGCTTTTTCCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCt                                                                                   <  1:234081/251‑1 (MQ=255)                                                                                                                                                                   ccgAACCTGTCCAGTCGCTGGAGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGCCCATGACAGGCTTCTTTTTCCGTAT‑CGTGGAAAGCATTATCATTTTTATTGGTAGCTAACACAATAGTTTCTGAGCTGAGCCGAGAAGTT‑AATGTTGAACAAATCTCATGTTTCGTGGTGGTCGCTTTTACCA‑CAGATGCTTTGATGCCAGTATGGTTTGTTGAATGTTTATTAAATCTg                                                                                  <  2:421235/249‑1 (MQ=255)                                                                                                                                                                     gAACCGGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTTGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGaa                                                                                                 <  2:396843/234‑1 (MQ=255)                                                                                                                                                                           gTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGa                                                                            <  2:62243/249‑1 (MQ=255)                                                                                                                                                                                gTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGGTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTc                                                                     <  2:271520/251‑1 (MQ=255)                                                                                                                                                                                          gggTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGTGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAAGCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTGGAGCGTGGCGGGGGCAAgg                                                           <  2:64139/250‑2 (MQ=255)                                                                                                                                                                                                    ggcggGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGagcagc                                                  >  1:353037/1‑250 (MQ=255)                                                                                                                                                                                                       gggTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAg                                              <  1:214865/251‑1 (MQ=255)                                                                                                                                                                                                        ggTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTAACA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGt                                             <  2:581189/251‑1 (MQ=255)                                                                                                                                                                                                             tCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaa                                         <  1:385649/250‑1 (MQ=255)                                                                                                                                                                                                                  cgcgTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtg                                   >  1:18948/1‑251 (MQ=255)                                                                                                                                                                                                                    cgTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcg                                 <  2:384199/251‑1 (MQ=255)                                                                                                                                                                                                                    cgTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcg                                 >  2:542527/1‑251 (MQ=255)                                                                                                                                                                                                                     gTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgt                                <  2:18948/251‑1 (MQ=255)                                                                                                                                                                                                                         cgcgGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTGTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTGTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtgcg                             <  2:288667/250‑1 (MQ=255)                                                                                                                                                                                                                            gggAATGGGTGCGACCATGACAGTCTCCTTTGTCTGTAT‑CGTGGAAATTATTTTCATTTTTATTGTTAGCTAATGCAATAGTTCCTGATCTGATCCGCTGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGTCAGTATGGTTTGTTGAACTTTTATTAAATCTGGGTTGATCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAg                          <  1:518395/250‑1 (MQ=255)                                                                                                                                                                                                                             ggAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCt                                                                                   >  1:533444/1‑192 (MQ=255)                                                                                                                                                                                                                             ggAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCt                                                                                   <  2:533444/192‑1 (MQ=255)                                                                                                                                                                                                                                        gACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGCAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTAATGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATTTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAACAACGTGGAACCACTGCGTGCGGAGTCAAATGAACGt              >  2:374724/1‑250 (MQ=255)                                                                                                                                                                                                                                        gACCATGACAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGt              >  1:405936/1‑250 (MQ=255)                                                                                                                                                                                                                                               aCAGTCTCCTTTGTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTCAGCGTGTCGGGAGCAAGGGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGt      <  2:93390/251‑1 (MQ=255)                                                                                                                                                                                                                                                cAGTCTCCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTg     <  2:414295/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gTCTCCTTTTTCTGTAT‑CGTGGCCACCATTTTCATTTTTATTGTTCGCTAATGCAATAGTTACTGAACTGATCCGATGAGCT‑AATGTTGAACAAATCTCCTGTTGAGTGGTGGCCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCCAAGTGGACCAACTGCGTGCGCCGTTAAATGAACGTATTCTGGTGCt   >  2:345653/1‑251 (MQ=255)                                                                                                                                                                                                                                                   tctcCTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTg  >  1:18577/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          CAGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTAT‑CGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTT‑AATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCA‑CAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTG  >  NC_000913/4223392‑4223884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑