Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,222,743	C→T	intergenic (‑256/+61)	arpA ← / ← iclR	regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,222,743	0	C	T	98.2%	178.6 / NA	55	intergenic (‑256/+61)	arpA/iclR	regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (30/24);  minor base G (0/1);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CATTGTTTATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACCCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTC  >  NC_000913/4222498‑4222986                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    cATTGTTTATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGACATATCATAAATCGCTAAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGCGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGCCATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTAt                                                                                                                                                                                                                                                >  2:304471/1‑251 (MQ=255)     gTTTATGGATGTCTTGAATTTTAACGATCAGAACGTTTAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGTTGGAATGAGATCTTGCGATTTTGTTAATAGTTAAGTGGTTTGATTGTTAAAAATAACGCTGAGTTAATAAAGGGGTGTGACGTGTATGACAGTAAGGTATTTTGGCGTGAATACGTCTAACAGGCTCGCTTATTCTa                                                                                                                                                                                                                                            <  2:29533/251‑1 (MQ=255)      tttATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATATAATATGATTACAACTAAAGAAATATCATAAATCGCTCCATCTCATAATGCAGCAGTAACAGATTCGGTGGAATGAGATCTTGCGATTTTATTACTAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGCAATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTAt                                                                                                                                                                                                                                           >  1:341976/1‑251 (MQ=255)                   tGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAg                                                                                                                                                                                                                                 <  2:148506/248‑1 (MQ=255)                      aTTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTatg                                                                                                                                                                                                                            >  2:462599/1‑250 (MQ=255)                         ttAACTATCAGAACCGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATgg                                                                                                                                                                                                                        >  1:414141/1‑251 (MQ=255)                               aTCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAg                                                                                                                                                                                                                    <  1:281143/249‑1 (MQ=255)                               aTCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGa                                                                                                                                                                                                                   >  1:134384/1‑250 (MQ=255)                                 cAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAata                                                                                                                                                                                                                <  1:391782/251‑1 (MQ=255)                                  aGAACATTGAAAAAATTTAATATGATGACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGTCGTAAAAGTTTCGGTGGAATGATATCTTGCGATTTGGTTAATGATTAAGTGGTTTGATGGTTAAAAATAATGCTGAGTTAATAGAAGTGTGGGAAGTGTATGGCATTAGGGTATTTTGGCTTCGATGCGATTAACAGACACTGGTATGCTATTGCCGCTCAGTTATGGTGGGCAGAatat                                                                                                                                                                                                               <  2:576981/251‑1 (MQ=255)                                      cAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGACTGAGATCTTACGATTTTGTTACTAATTAAGTGGTTTGATGTTTAAAAATAATGCTGGGTTAATAAAAGTGAGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGAATGATGGGCAGAATATTGcc                                                                                                                                                                                                           >  1:530111/1‑251 (MQ=255)                                          gAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTg                                                                                                                                                                                                       >  1:82964/1‑251 (MQ=255)                                          gAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTg                                                                                                                                                                                                       >  1:570089/1‑251 (MQ=255)                                           caaaaaTTTCATAAGATTAAAACTAAAGAAATCTCATACATCGATAAATCTCATAATGCAGCCGTCACAGTTTCGGAGGACTGAGATCTTACGATTTTGTAAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGAGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGAGCTTAACAGACACTCTTATTCTATTGCTACTCAGGTATGATGGACAGAATATTGCctcttc                                                                                                                                                                                                      >  2:45408/2‑249 (MQ=255)                                           aaaaaaTTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGc                                                                                                                                                                                                      >  1:482613/1‑251 (MQ=255)                                               aaTTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGc                                                                                                                                                                                                  <  1:387854/251‑1 (MQ=255)                                                 tttAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGTTTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGcc                                                                                                                                                                                                 >  2:139025/1‑250 (MQ=255)                                                   tAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAg                                                                                                                                                                                               <  2:139957/250‑1 (MQ=255)                                                       atGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGaaaa                                                                                                                                                                                           >  1:370157/1‑250 (MQ=255)                                                          aTTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGTAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTc                                                                                                                                                                                       <  1:312504/251‑1 (MQ=255)                                                                           atCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAA‑TAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACg                                                                                                                                                                     <  1:124238/251‑1 (MQ=255)                                                                            tCATAAATCGCTCAATCTCCTAATGCCGCCGCAAACGTTTCGGTGGACTGCGATCTTGCGATTTTGTTCATAATTAAGTGGTTTGATGTTTAAACATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACg                                                                                                                                                                     >  2:59438/1‑251 (MQ=255)                                                                            tCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACg                                                                                                                                                                     >  1:247268/1‑251 (MQ=255)                                                                                  aTCGCTCTATCTAATAATGCAGCCGTACTAGTTTCGGTGGACTGAGAACTTGCGATTTTGTTAATCATTAAGAGGTTTGATGTTTAAAACTAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGCCATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTATTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAACAAGTCAGCGCATTCCACCGTACGCCAGCg                                                                                                                                                               >  2:44801/1‑251 (MQ=255)                                                                                       tCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCAct                                                                                                                                                          >  2:469384/1‑251 (MQ=255)                                                                                         aatctaaaaaTGCTGCCGTAAAAGGTTTGGGGGAATGAAATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCCCCGTACGCCAGCGTCAcgtc                                                                                                                                                        <  2:23922/243‑4 (MQ=255)                                                                                                                     ggTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGcc                                                                                                                             >  1:496394/1‑250 (MQ=255)                                                                                                                                    tGCGATTTTTTTAATAATTAAGTGGTTTGATGGTTAAAAATAATGCTGAGTTAAAAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATTCGATTAACAGACACTTTTATTTTATTGCCACTCAGGTATGATGGGCAGAATATTGCCGCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTGCGCCAGCGTCACTTCCGTCGCCGCTTTAATCACCagctcgccttaccgggtcacg                                                                                                                <  2:374438/248‑21 (MQ=255)                                                                                                                                     gCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGt                                                                                                            >  1:130956/1‑251 (MQ=255)                                                                                                                                     gCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGt                                                                                                            >  2:511146/1‑251 (MQ=255)                                                                                                                                           ttGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCg                                                                                                       <  1:392510/250‑1 (MQ=255)                                                                                                                                            tgttactaaaaaaacggTTTGATGTTTAACAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCACTTAACAGACACTATTATTATATAGCCAATCAGGTATGATAGGAAGAGTATTGCCTCTGCCCGACAGAAAAATACAGAGCATTCCACCGTACGCCAGCGTCACTTCCTTAGCCGCTTTAACCACAATCGCGCCAAACTCGGTCCCGCGGCCATAGGt                                                                                                     >  2:525868/16‑251 (MQ=255)                                                                                                                                                      tAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGa                                                                                            <  1:271955/250‑1 (MQ=255)                                                                                                                                                       aaGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGaa                                                                                           <  1:1852/250‑1 (MQ=255)                                                                                                                                                          tGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCg                                                                                       >  1:442296/1‑251 (MQ=255)                                                                                                                                                           ggTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCg                                                                                       <  2:557973/250‑1 (MQ=255)                                                                                                                                                              ttGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTACCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTcc                                                                                   >  2:86534/1‑251 (MQ=255)                                                                                                                                                                        aaaaTAATGCTGAGTTAAGAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCTCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGaa                                                                         <  1:27154/251‑1 (MQ=255)                                                                                                                                                                         aaaTAATGCTGCGTTAATAAAAGTGTGTGAAGTGTATGCCATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGaa                                                                         >  2:84469/1‑250 (MQ=255)                                                                                                                                                                         aaaTAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGaaa                                                                        <  1:180033/251‑1 (MQ=255)                                                                                                                                                                          aataatGCTGAGTTAATAAAAGTGTGTGACGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATAcagctcggaagcgagtcgtgtaggg                                                                        >  2:434019/1‑226 (MQ=255)                                                                                                                                                                          aataatGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATAc                                                                                                <  1:434019/226‑1 (MQ=255)                                                                                                                                                                                   taGTTAATAAAATGGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCATTGCGATTAACAGCCACTCTTATTCTATTGCCACTCAGGTATTATGGGCATAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGATATAGAAATTGCGGCa                                                                <  2:74985/248‑1 (MQ=255)                                                                                                                                                                                         ataaAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTc                                                        >  1:35285/1‑251 (MQ=255)                                                                                                                                                                                           aaaaGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCAc                                                      >  1:242574/1‑251 (MQ=255)                                                                                                                                                                                                         tgtATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGaa                                         >  2:481852/1‑250 (MQ=255)                                                                                                                                                                                                                       ttATTTTGGCGTAAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCa                            >  1:224394/1‑249 (MQ=255)                                                                                                                                                                                                                         aTTTTGGCGTCAATGCGATTAACATACACTCTTATTCTAGTGCCACTCAGGTATGATGGGCAGAATAGTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGGGCCAAACTCTGTCACTCGGTCATCGGTAATACGGGAAATCGGTCCTGAAATAGAAAGTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTTCAAgg                         <  2:235579/250‑1 (MQ=255)                                                                                                                                                                                                                         aTTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAgg                         <  1:481852/250‑1 (MQ=255)                                                                                                                                                                                                                               gCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTa                  <  1:469384/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTCAATGCGATTAACAGACTCTCTTATGCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACGCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGc                <  2:130956/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaTGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGAAGCATATTGCCTCTGCACGCCAGAAAAAGTCAGCGCATTCCACCGTACGCAAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCACACTCGGTCACGCGGTAATCGGTAATACGTGACATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTAACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGcccc             >  1:280139/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGCGATTACCAGACACTCTTATACTATTGCCACTCATGTATGATGGGCAGAATATGGCCTCTGCCCGCCAGAATAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGATATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACTCGCTGCAAGGCAACGGAGCCCCa            <  2:209008/250‑1 (MQ=255)                                                                                                                                                                                                                                         gATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGc        >  2:459071/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCa       <  1:462599/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gacccccTTATTCTATTTCCACTAAGGTATGATGGGCAGAATAGTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCTCCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCGCGGTGCTCATCTAAAATACACGCTGCAAGGCATCGGAGCCCCattggttgttc  <  2:389582/245‑11 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    CATTGTTTATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACCCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTC  >  NC_000913/4222498‑4222986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑