| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,219,752 | C→T | S386F (TCC→TTC) | aceK → | isocitrate dehydrogenase kinase/phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,219,752 | 0 | C | T | 96.9% | 199.8 / NA | 64 | S386F (TCC→TTC) | aceK | isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (30/32); minor base G (0/2); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTC > NC_000913/4219506‑4219997 | aTCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCGGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTtccc < 2:487459/250‑1 (MQ=255) tACAGGGCTGTAATGAGAAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCCTATTTTCCCGGc > 2:253283/1‑250 (MQ=255) aCAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTAACAACTGGTTAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACGTTGTGCTGGAGAAGCGGCATATTTTCCCGGc < 2:578470/249‑1 (MQ=255) cAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCAtt > 2:216079/1‑251 (MQ=255) gCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATg < 1:546332/251‑1 (MQ=255) gTAATGAGCAGTTCATTGAAGCTCCGGGTATTCGTGGAATGTTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCTCGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCATGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGTCATTAATgg < 1:106861/249‑1 (MQ=255) gTAATGAGCAGTTCATTGAAGCGCCGGGTCTTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGAATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGa > 1:481174/1‑250 (MQ=255) gTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGa < 1:168929/250‑1 (MQ=255) tggCAGTTCATGGAAGCTCCGGGGATTCGTGGAATGGTGATGTTGGTGTGTACGCTGTCTGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCATAAAGATAGGGCTGCCGCTCACGTTCCTGCCTGCTATCAACTGGTGAAAGAGCACGAGCGCGGGGCCCTTATGGCGTACACCCAGGAGTTGGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTAc < 1:563147/248‑1 (MQ=255) gagcctttCATTGAAGCGCCGGGTATTCGGGGAATGGTGATGTTGGTGTATACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCCTCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCGCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCGGGAGAAGCGGCATATTTTCCCGGCATTAATTGAATTACt < 2:157360/245‑1 (MQ=255) gAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACTATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCTGGCATTAACGGAATTAc < 2:301817/250‑1 (MQ=255) cgTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCtt > 2:149697/2‑251 (MQ=255) cATTGAAGCGCAGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGCTCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCCTATTTTCCCGGCATTAATGGAATTACTGCTTCAgg > 2:581454/1‑251 (MQ=255) aTTGGAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAgg > 2:159554/1‑250 (MQ=255) cgcCGGGTATTCGGGGCATGGGGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGAGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGTCTGCTATCAACTGGGGAAAGAGCACGATCGCGTGGGCCGCATGGCGGACACCCAGGAGCTTGAAAACTCTGTTCTGGAGCAGCGGCATATTTTCCCGGCATTCATGGAATTTCGGCTTCAGGAA‑GAAGCg > 2:341138/1‑251 (MQ=255) cGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGaa < 1:534913/251‑1 (MQ=255) gggTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTGCGTGCCTGCTATCAACTGGTTAAAGAGCACGATCGCGTGGGCCGAATGGCGGACATCCATGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGaaa < 1:67127/251‑1 (MQ=255) aTTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAAt > 1:126684/1‑251 (MQ=255) tagAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTAAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCCTATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATAACCg > 1:268162/3‑251 (MQ=255) gTGATTTTGGGGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGGCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCTGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCgg < 2:414641/251‑1 (MQ=255) gTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCgg > 2:534046/1‑251 (MQ=255) gATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCg > 1:316981/1‑251 (MQ=255) gATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCg > 1:556914/1‑251 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTGCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAGCAAAtt < 1:113309/250‑1 (MQ=255) ccGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCCGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGAATATTTTCCCGGCATTAATGGAATTACTGCATCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGca > 1:94697/1‑249 (MQ=255) ccGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCAGTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCTAACAAATTGTG‑ATTCGCCa < 2:360980/251‑1 (MQ=255) ttGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTta < 1:302498/250‑1 (MQ=255) ttGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTta < 1:89443/250‑1 (MQ=255) tGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTtat > 1:168743/1‑250 (MQ=255) cAAAGTCATCAAAGCCAGGTTCGCGCCGCAGAAAGAGATGACTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑TCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCTGCGG‑a < 2:246303/251‑1 (MQ=255) gTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGt < 1:159554/251‑1 (MQ=255) tcatcaAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTg > 2:369720/1‑251 (MQ=255) catcaAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTg < 1:357846/250‑1 (MQ=255) atcaAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGcc < 1:276593/251‑1 (MQ=255) cgcgcccaaGAAAGAGATGTCTGCCGCTCACGTTAGTGCCTGCTATCCACTGGAGAAAGCGAACGCTCGCGTGGGCCGACTGGCGGAAACCAAGGAGTTTGAAAACTTTGTGCTGGAGCAGCGGCATATTTTCCCGGCATTCATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGCACAAATTGAG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAatatttgac > 2:532460/9‑246 (MQ=255) aaGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAACGAGCACGCTCGCGTGGGCCGAATGGCGGACACCCAGGAGCTTGAACACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATATTTATATTGAGAGGCGG‑GTGGTGCCGCTTCATATTTGGCTGGAACAAGTg > 2:229442/1‑251 (MQ=255) agaTGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCTCCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGaa < 1:253283/251‑1 (MQ=255) agaTGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGaa < 2:141888/251‑1 (MQ=255) cggCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTca < 1:23749/248‑1 (MQ=255) gCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTcag‑ca < 1:369720/251‑1 (MQ=255) gCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTcag < 1:433175/249‑1 (MQ=255) gCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATCTTTTCCCGGCATTAATGGAATTCCTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCGTCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGGCAG‑CAGtt > 1:76169/1‑251 (MQ=255) cACGGTCGTGCCCGCTATCAACTGGTGAAAGAGCACGAGCGCGGGGGCCGAATGGCGGACTCCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTGCCCGGCATGAATGGAATTACTGCGTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑AGTCGCCATCTTTATATTGAGCGGCGT‑ATGGTGCCGCTCAATATCGGGCTGGATCATGTTGAAGGTcctcagctgcg < 2:231443/251‑11 (MQ=255) cGTTCGGGCCTGCTTTCAACTGGTGAAAGAGCACGATCGCGTTGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCTGCATATTTTCCCGACATTAATGGAATTACTGCTTCAGGAA‑GCATCGGAAAAAATCACCGATCGCTGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGAAAGGTCAG‑CAGTTGcgcg < 2:266351/251‑1 (MQ=255) gTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGcgcg > 1:360620/1‑250 (MQ=255) gCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTg < 2:126684/249‑1 (MQ=255) aTCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGGGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTTCGCGACGCCATTgaaga > 1:234655/1‑250 (MQ=255) ggTGAAAGGGCACGATTGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGTCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAAGTGTG‑ATTCGCCAGCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACATGTTGAAGGTCAG‑CAGTTGCGTGACGCCGTTGAAGAATACGGt < 2:248203/250‑1 (MQ=255) gAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACg < 2:360620/251‑1 (MQ=255) gagCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATG‑AATTTCTTCCTTAGGAAAGC‑GCGGGAAAAATTACCGGACTCGGGCAACAAATTTTGGATT‑GCCATCTTTAAATTTGGCCGCGGGAGGGGGCCGCCCATTATTTGGCTGGAA‑AAGTGGGAGGGCAGGCAGTTGCCGGAC‑CCCTTGAAGAATAAGGGAAAGCTAtt > 2:124156/1‑251 (MQ=255) gCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTc > 1:502609/1‑251 (MQ=255) gCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTc < 2:5005/251‑1 (MQ=255) gggggacgacaggCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGGGAAGCGGCATATTTTCCCGGCATTAATGAACTTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGAGCCGCTCAATATCTGGCTGGAACAAGGGGAAGGTCAG‑CAGTTGCGCGACGCCATTTAAGAATACGGTAACGCCATTCGCCAGCTTGc > 2:238669/12‑251 (MQ=255) aaTGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTa < 1:547328/251‑1 (MQ=255) acCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTccc > 1:219485/1‑251 (MQ=255) acCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCACGGCATTAATGGAATTACTGCTTCACGAA‑GCAGCGGAAACAATCACCGATCTCGGCGAACAAATTGTG‑ATTAGCCATCTTTATCTTGTGCGGCGG‑ATAGTGCCGCCCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGCAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTccc > 1:524711/1‑251 (MQ=255) tttGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACAt > 1:16641/1‑251 (MQ=255) tGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATg < 1:239768/250‑1 (MQ=255) aaaaCTTTGTGCTGGAGAAGGGGAAGATTTGCCCGGCATAAATGGAAGTACAGCTACAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCGGCTCAATAGCTGGCTGGAACAAGTAGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGACAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTg < 2:20381/251‑1 (MQ=255) tttGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATAACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTAAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGGCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTa > 1:288469/1‑250 (MQ=255) ggAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGCC‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAAAAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCg > 2:369553/1‑251 (MQ=255) agaAGCGGCATAGTTTCCCGGCATTTATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGAGCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGTAACAAGTGTAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGTCGACATGCTGTTTAAAAACTTCGgt < 1:233674/251‑1 (MQ=255) cATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGTAAAAAATCACCGATCTCGGCGAACAACTTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGAGGAAGGTCAG‑CAGTTGCGCGTCGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACCTTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCg > 2:102007/1‑251 (MQ=255) atatTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATTTGGCTGGAACAAGTGGAAGGGCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGACAGCTTGCCGATGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTTGGTGTAAccagt > 2:514541/1‑248 (MQ=255) atTTTCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGGGCCGCGCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTc < 2:491557/250‑1 (MQ=255) | ATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAA‑GCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTG‑ATTCGCCATCTTTATATTGAGCGGCGG‑ATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAG‑CAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTC > NC_000913/4219506‑4219997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |