Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,099	C→T	intergenic (‑130/‑223)	yifB ← / → ilvL	putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,099	0	C	T	100.0%	133.0 / NA	44	intergenic (‑130/‑223)	yifB/ilvL	putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/26);  total (18/26)

GTAAGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTG  >  NC_000913/3949858‑3950342                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    gTAAGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGGACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGGGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCaaaaa                                                                                                                                                                                                                                            <  1:58212/251‑1 (MQ=255)   aaGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTTGAATGATGTTCGCAAAAATg                                                                                                                                                                                                                                          >  1:505207/1‑251 (MQ=255)                ttAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTTGCAAAAATGCAGCGGACAAAgg                                                                                                                                                                                                                             >  2:32119/1‑251 (MQ=255)                ttAAGCCGGGTAGACCTTTACTGATATATACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGCATGATGTTCGCAAAAATGCAGCGGACAAAgg                                                                                                                                                                                                                             >  1:231750/1‑251 (MQ=255)                        ggTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACt                                                                                                                                                                                                                      >  1:505513/1‑250 (MQ=255)                         gTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGGTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTAc                                                                                                                                                                                                                    <  2:464964/251‑1 (MQ=255)                               cTTTTCTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCAACATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCACGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGGACTACGAGGa                                                                                                                                                                                                               >  1:388485/1‑250 (MQ=255)                                   aCTGAGATGTACCTCAACAGTGATCGGGTGCGCATTTACGCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGATTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTTAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTTCGAGGAAggg                                                                                                                                                                                                           <  2:378413/250‑1 (MQ=255)                                      gATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGaac                                                                                                                                                                                                        <  1:288836/250‑1 (MQ=255)                                             tcctgtaCAGTGCTGGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCTCTCCTTGAGTCAGCATTATGTGCATTAGATATCGCGGCTGTAGCCCGCTAATTCGTGAATGTTAGTGGCTGATTCCTTTTTATTTTGGCAAGTGAAGTTTAGTTGTTCTGGCGGTTGAATGATGTCCTCAAAAATGCAGCGGAGAAAGCATGAATGTCGAGGAAGGGTTCAACATTc                                                                                                                                                                                                 <  2:292647/244‑1 (MQ=255)                                                       tGATCGGGGGCGCATTTACTACCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGTATGATGTTCGCAAAAATGCAGCGGACAAAGGAAGAATTACGAGGATGGGAACAACATTCAGACTGAAAtt                                                                                                                                                                                      >  2:423898/1‑251 (MQ=255)                                                                   cATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTtca                                                                                                                                                                          <  1:32119/251‑1 (MQ=255)                                                                        acccccATGGCTGCGCGTGTATGAACAATTGACAGTGACAGAATCCCGCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCGGGCTGTGGAATGATGTTCGCAAAAATGCAGCGGAAAAAGGATGAACTACGAGGAAGGGAACAACAGTCATACTGAAATTGAATTTTTTtcactca                                                                                                                                                                      <  2:350597/247‑1 (MQ=255)                                                                        aCTCCCATGGCTGCGCGTGTATGAACAATTGACAGTGACATAAGCCCTCCGTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTtcactca                                                                                                                                                                      <  2:151313/250‑1 (MQ=255)                                                                           cccAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTctctcact                                                                                                                                                                    <  1:511810/249‑1 (MQ=255)                                                                             cAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtattt                                                                                                                                                                <  1:13676/251‑1 (MQ=255)                                                                               gggCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTGGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAATAACATTCATACTGAAATTGAATTTTTTTCACTCACtatttta                                                                                                                                                              <  2:496311/251‑1 (MQ=255)                                                                                                      gACAGTGACATAAGCCCTCCTTGAGTCTCCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat                                                                                                                                        <  1:432197/250‑1 (MQ=255)                                                                                                        cAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATAtt                                                                                                                                     <  1:528523/251‑1 (MQ=255)                                                                                                         aGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAAAAATTTATAttt                                                                                                                                    >  2:207873/1‑250 (MQ=255)                                                                                                               aTAAGCCCTCCTTGAGTCATCATTATGTGCATAAGATATCGCTGCTTTAGCCCTCTAATTCGTGAATTTTAGTGGCTGATTGCTGTTTATGTGTGCAAGTGAAGAGGAGTTGTTCTGGCGGTGGAAGGATGTTCGCAAAAATGCAACGGACAAAGGAAGAACTACGAGGTAGGGAACAACAGTCAAAATGAAATTGAATTTTTTTCACTCACTATTTTAATTGTAAAAAACAACAATTCATATTGAAatta                                                                                                                              <  2:44264/251‑1 (MQ=255)                                                                                                                                         gagCATCAGATATCGATGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCACGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCCAAAATGCAGCGGACAAAGGGTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGTAATTATTAAACGCCTCATAAAAATCGGcaa                                                                                                     >  2:90551/3‑248 (MQ=255)                                                                                                                                           gCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGTAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa                                                                                                   <  1:574383/250‑1 (MQ=255)                                                                                                                                            cATAAGCTATCGCTGCTGTAGCCCGCTAATTCGTGCATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGTAATGCTGTTCGAAAAAATGGAGCGGACAAAGGATGAACTCCGAGGAAGGGAAAAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAAAAAACTTTATATTGAAATTATTAAACTCTTAATAAAAATCGGCCaaaa                                                                                                  >  2:212656/1‑250 (MQ=255)                                                                                                                                              tAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGCGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGCTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAat                                                                                               >  1:267123/1‑251 (MQ=255)                                                                                                                                               aaGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATGCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACGCACTATTTTATTTTTAAAAAACAACAATGTATATTGAAATGATTAAACGCATCATAAAAATCGTCCAAAAAata                                                                                              <  2:84265/251‑1 (MQ=255)                                                                                                                                                        ctgctgCAGCCCGCTACTTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGCGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGCGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt                                                                                     >  2:550813/1‑251 (MQ=255)                                                                                                                                                              tAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTAc                                                                               >  2:168711/1‑251 (MQ=255)                                                                                                                                                                       aaTTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTa                                                                       <  2:402141/250‑1 (MQ=255)                                                                                                                                                                           cGTGAATTTTAGTGGCTGATTCCTGTTTAGTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTa                                                                  <  1:553147/251‑1 (MQ=255)                                                                                                                                                                            gTGACTTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCTGTGGCATCATGTTCGCAAAAATGCAGCGGACAAAGGATGACCTACGAGGAAGGGAACAACCTTCATACTGAAATTGAATTTTTTTAACACACTTTTTTATTTTTAACAAACACCAATTTATATTTCAATTATTAAACGCATCATAAAAATCCGCCAAAAAATCTCTTGTACTATTTACAAAACCTATGGTa                                                                  >  2:227572/1‑250 (MQ=255)                                                                                                                                                                                         gCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCAtt                                                    <  1:235769/251‑1 (MQ=255)                                                                                                                                                                                          cTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTc                                                   >  1:26392/1‑251 (MQ=255)                                                                                                                                                                                             aTTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCtt                                                <  2:505207/251‑1 (MQ=255)                                                                                                                                                                                                  tGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc                                                                                                                   <  1:109868/179‑1 (MQ=255)                                                                                                                                                                                                  tGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc                                                                                                                   >  2:109868/1‑179 (MQ=255)                                                                                                                                                                                                          tgtgCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCATTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGc                                    <  1:231338/250‑1 (MQ=255)                                                                                                                                                                                                              cAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt                                                                                                                                          <  2:100928/144‑1 (MQ=255)                                                                                                                                                                                                              cAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt                                                                                                                                          >  1:100928/1‑144 (MQ=255)                                                                                                                                                                                                                                   ggCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGccc             <  1:131003/248‑1 (MQ=255)                                                                                                                                                                                                                                   ggCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCGATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTc          <  2:218329/251‑1 (MQ=255)                                                                                                                                                                                                                                      ggTGGAATGATGTTAGCAAAAATGCAGCGGCCCACGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGACCAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTAc       >  2:556475/1‑251 (MQ=255)                                                                                                                                                                                                                                           aaTGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTg  <  1:503611/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaTGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTg  >  1:240283/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    GTAAGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTG  >  NC_000913/3949858‑3950342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑