Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,946,246 G→A noncoding (2543/2904 nt) rrlC → 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,946,2460GA96.2% 184.0 / 1.2 107noncoding (2543/2904 nt)rrlC23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (51/50);  total (55/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 107 total aligned reads displayed.

CCTGGTCGGACATCAGGAGGTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTG  >  NC_000913/3946003‑3946473
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    
ccTGGTCGGACATCAGGAGGTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCAACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATgg                                                                                                                                                                                                                               >  1:550426/1‑251 (MQ=14)
ccTGGTCGGACATCAGGAGGTTAGTGCAATGGCATAAGCCAGCTTGACTGCCAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGCTCCGGTGGTTCTGAATGGTAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGAGTTTGGCACCTC‑GATGTCGGCTCCTCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATgg                                                                                                                                                                                                                               >  1:297873/1‑251 (MQ=255)
      cGGACATCAGGAGGTTAGTGCAATTGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGATCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCTCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGTCGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGTAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGtt                                                                                                                                                                                                                          <  1:580298/250‑1 (MQ=255)
      cGGACATCAGGAGGTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTc                                                                                                                                                                                                                         <  2:441809/251‑1 (MQ=14)
       gaaCATCAGGAGGTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAC‑GTGAGTCCCAAAGGTATGGCTGGTCg                                                                                                                                                                                                                        >  2:559344/3‑251 (MQ=255)
        gACATCAGGAGGTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAG‑GTAGGTCCCAAAGGTATGGCTGTTCGc                                                                                                                                                                                                                       <  1:36450/251‑1 (MQ=14)
            tCAGGAGGTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCAtt                                                                                                                                                                                                                   >  1:107521/1‑251 (MQ=14)
              aggaggTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGCGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCAttt                                                                                                                                                                                                                  >  1:478448/1‑250 (MQ=14)
                 aggTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAg                                                                                                                                                                                                              <  1:323759/251‑1 (MQ=14)
                  ggTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAgg                                                                                                                                                                                                             >  1:53060/1‑250 (MQ=14)
                      aGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGt                                                                                                                                                                                                          >  1:78920/1‑250 (MQ=14)
                       gTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAACGTGGTAc                                                                                                                                                                                                        >  1:362318/1‑251 (MQ=14)
                          cAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGCTACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTAcgcg                                                                                                                                                                                                     >  1:301771/1‑251 (MQ=14)
                            aTGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAg                                                                                                                                                                                                   <  1:163563/251‑1 (MQ=14)
                            aTGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAg                                                                                                                                                                                                   <  2:460109/251‑1 (MQ=14)
                               gaaTAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGCTCGCCATTTAAAGGGGGACGCGAGCTg                                                                                                                                                                                                >  2:456278/3‑251 (MQ=9)
                               gCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTg                                                                                                                                                                                                <  2:583181/251‑1 (MQ=14)
                                cATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCAAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTgg                                                                                                                                                                                               <  1:4822/251‑1 (MQ=14)
                                cATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTATGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCCACGGGATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTgg                                                                                                                                                                                               >  2:207925/1‑251 (MQ=9)
                                cATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGATCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGCA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGGACGCGAGCTgg                                                                                                                                                                                               >  2:521904/1‑251 (MQ=9)
                                 aTAAGCCAGCTTGACTGAGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGCATGGCTGTTCGCCATTTAAAGGGGTACGCGAGCTggg                                                                                                                                                                                              >  1:29318/1‑251 (MQ=9)
                                      ccAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAg                                                                                                                                                                                         <  1:63642/251‑1 (MQ=14)
                                       cAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGttt                                                                                                                                                                                           >  1:245102/1‑248 (MQ=14)
                                              aCTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcgt                                                                                                                                                                                 >  1:190444/1‑251 (MQ=14)
                                              aCTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcgt                                                                                                                                                                                 >  1:318986/1‑251 (MQ=14)
                                                 gCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTg                                                                                                                                                                                >  1:396623/1‑249 (MQ=14)
                                                   gAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCTCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGAc                                                                                                                                                                            <  1:206219/251‑1 (MQ=14)
                                                      cGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGATCTGAA‑TTAGGTCCCAAAGGTATGGCGGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGt                                                                                                                                                                         <  1:268311/251‑1 (MQ=9)
                                                      cGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGGCGTGAGACAGt                                                                                                                                                                         <  1:429233/251‑1 (MQ=14)
                                                      cGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCGC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGt                                                                                                                                                                         <  2:430150/251‑1 (MQ=14)
                                                       gTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGtt                                                                                                                                                                        <  2:31778/251‑1 (MQ=14)
                                                        tgccGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTAATCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGCAGGCGGTGTTTGGCACCTC‑GATGTCGGCACATCCCATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCCTTTCAAGTGGTAAGCGAGCGGGGGTTAGAAcgtcgggggacatttc                                                                                                                                                                       >  2:404747/4‑240 (MQ=11)
                                                         gACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGGA‑GTAGGTCCCACAGGTATGGCTGTTCGCCATTTAAAGCGGTACGGGAGCTGGGTTTTAACCGTCGTGAGACAGTTCg                                                                                                                                                                      >  2:91197/1‑251 (MQ=11)
                                                         gACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCg                                                                                                                                                                      <  2:53060/251‑1 (MQ=14)
                                                           cGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGt                                                                                                                                                                    >  1:225220/1‑251 (MQ=14)
                                                                     aGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATCCCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGc                                                                                                                                                          <  2:538183/251‑1 (MQ=14)
                                                                          cGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTgg                                                                                                                                                     <  1:341186/251‑1 (MQ=14)
                                                                                   gTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGgag                                                                                                                                            <  1:241948/251‑1 (MQ=14)
                                                                                     cATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGGGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGgaga                                                                                                                                           >  2:511670/1‑250 (MQ=14)
                                                                                           gATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAgg                                                                                                                                    >  1:14795/1‑251 (MQ=14)
                                                                                             tCCGGTGGGTCTGAATGGAAGGGCCATCGCTCAACGGATAAACGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAC‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGACACCGTTCGGTCCCTATCTGCCGGGGGTGCTGGAGAACTGAgggg                                                                                                                                  >  2:510962/1‑251 (MQ=11)
                                                                                                ggtggtTCTGAATGGAAGGGCCATCGCTCAACGGATAACAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGTGACCGTTCGGTCCCTATCTGCCGTGGGCGCTGGGGAACTGAGGGGGgc                                                                                                                               >  2:106335/1‑251 (MQ=9)
                                                                                                 gtggtTCGGAATGGAAGGGCCAAAGCGCAACGGATAAAAGGTACGCCGGGGATAAAAGGCTGATACCGACCAAGAGTTCATATCGACGGCGGTGTTTGGCAGCTC‑GATGTCGGCTCATCACAGCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGGTGTTCGCTAGTTAAAGTGGTACGCGAGCTGGGTTTAGAGCGGCGTGAGACAGTGCGGTCCCGATCTGGCGTGGGCGCTGGAGAACTGAGGGGGgct                                                                                                                              <  2:116936/251‑1 (MQ=11)
                                                                                                         gAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTa                                                                                                                       <  1:164348/250‑1 (MQ=14)
                                                                                                              gAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACgg                                                                                                                 >  1:104996/1‑250 (MQ=14)
                                                                                                                aGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACgaga                                                                                                               <  2:178373/251‑1 (MQ=31)
                                                                                                                     cATCGCTCAACGGATAAAAGGTACGCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCGCCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAATGTGGTACGCGATCTGGGTGTAGAACGTCGTGAGGCTGTTCGGTCCCTATCGGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGAcc                                                                                                          <  1:235214/251‑1 (MQ=11)
                                                                                                                        cGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATGCCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGGGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCgg                                                                                                        <  1:391457/250‑1 (MQ=14)
                                                                                                                        cGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGa                                                                                                       <  1:181935/251‑1 (MQ=14)
                                                                                                                         gCTCAACGGATAACAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGCACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCgg                                                                                                        >  1:377300/1‑249 (MQ=14)
                                                                                                                         gCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGGTCATATCGACGGCGGGGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCgg                                                                                                        >  1:32302/1‑249 (MQ=14)
                                                                                                                         gCTCAACGGATAAAAGGTACTCAGGGGTTAACAGGCTGTTACCACCCAAGAGTTCATATCGACGGCGGTGTTTGGC‑CATCCGATGTCGGCTCATCACATCCTGTAGCTGAA‑GTAGGCCCCAAAGGTAGGGCTGTCCGCCATTGAAAGTGGTACGCGAGCTGGTTTTAGAACGTCGTGAGACAGTTCGGTCCCTAGCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAg                                                                                                      <  1:291426/251‑1 (MQ=11)
                                                                                                                            cAACGGATAAAAGGTTCTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCTAGCTGGGTTTAGAACGGCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTATTACGAGAGGACCGGAGt                                                                                                     <  1:488736/249‑1 (MQ=255)
                                                                                                                             aaCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGa                                                                                                  <  1:425645/251‑1 (MQ=14)
                                                                                                                              aCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGCCCGGAGTGGAc                                                                                                 >  1:289498/1‑251 (MQ=14)
                                                                                                                              aCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGAc                                                                                                 <  1:207925/251‑1 (MQ=14)
                                                                                                                              aCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATAGCGACGGCGGTGTTTGTCACCTC‑TATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGGCGTGAGACAGTTCGGTCCCTATCTGCCGTTGGCGCTTGAGAACTGAGTGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGAc                                                                                                 <  2:550426/251‑1 (MQ=11)
                                                                                                                               cGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCGGAA‑GGAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGCGGTACGCGATCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGGGGGGGGCTGCTCCTAGTCCGAAAGGACCGGAGTTGACg                                                                                                >  1:97420/1‑251 (MQ=11)
                                                                                                                                ggATAAAAGGTACTCAGGGGATAACAGACTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTGGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGGAGGGCTGTTCGCCATTTAAAGGGTTACGCGAGGTGGGTGTAGAACGTCGTTAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTTGGGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACGGGCGAGGACGc                                                                                               >  2:586986/1‑251 (MQ=11)
                                                                                                                                    aaaaGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGGCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATc                                                                                            >  1:74856/1‑250 (MQ=14)
                                                                                                                                        ggTACTCTGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTAGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGGGTGGACGCATCCCT‑gg                                                                                      >  2:378573/1‑251 (MQ=9)
                                                                                                                                        ggTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑gg                                                                                      >  1:208147/1‑251 (MQ=31)
                                                                                                                                         gTACTCCGGGTATAACAGGCGGAGACCGCACAAGAGTTCATATCGACGGCGGGGTTTGGCGCCGT‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGGATGGCTGTTGGCCATTTAAAGTGTGACGCGAGTTGGGTATAGAACGTCGTGAGACAGTTCGGTCTTTATCAGCCGTGGGCGCTGGAGAATGGTGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCTCT‑Ggt                                                                                     <  1:360466/251‑1 (MQ=11)
                                                                                                                                         gTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGGGTGGACGCATCACT‑Ggt                                                                                     >  2:491036/1‑251 (MQ=14)
                                                                                                                                          tACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGGATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGGGAGGCCCGGAGTGGACGCATAACT‑Ggtg                                                                                    >  1:252280/1‑251 (MQ=9)
                                                                                                                                           aCTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑Ggtg                                                                                    <  2:90728/250‑1 (MQ=14)
                                                                                                                                              ccGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGAAGCATCACT‑GGGGTTCg                                                                                >  2:328754/1‑251 (MQ=14)
                                                                                                                                              ccGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GGAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCg                                                                                <  2:190444/251‑1 (MQ=14)
                                                                                                                                               cGGGGATAAAAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGAACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGGCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGGGTTCgg                                                                               >  2:268431/1‑251 (MQ=9)
                                                                                                                                                          ggCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGcc                                                                    >  1:485997/1‑251 (MQ=14)
                                                                                                                                                           gCTGATACCGCCCAAGAGTTCAGATCGACGGCGGTGTTTGGCACCTC‑GATGGCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGGGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCa                                                                   <  2:43956/251‑1 (MQ=9)
                                                                                                                                                               aTACCGCACAAGAGTTCAAATCGCCGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGCA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCCTTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGCCAGTTCGGTCCCTATCTGCCGGGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGGGAGGAACGGGGTGGACGCATCACT‑GGTGTTCGGGTGGTCATGCCACTgg                                                               >  2:379980/1‑251 (MQ=11)
                                                                                                                                                                 aCCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACGGAGGGGGGCTGCTCCGAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCa                                                             <  2:241756/251‑1 (MQ=14)
                                                                                                                                                                  ccGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGGGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCAc                                                            >  1:119036/1‑251 (MQ=14)
                                                                                                                                                                     cccAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCGCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGc                                                         <  1:379980/251‑1 (MQ=14)
                                                                                                                                                                     cccAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGGACGCGAGCTGGGTTTAGAACGTCGTGAAACAGTTCGGTCCCTATCTGCCGTGGGCGCGGGGGAACTGTGGGGGGCTGCTCCTCGTACGCGAGGACCGGAGGGGGCGCATCACT‑GGTGTTCGGGTTGGCATGCCAATGGCACTGc                                                         >  2:314200/1‑251 (MQ=11)
                                                                                                                                                                       cAAGAGTTCATATCGACGGCGGTGTTTGGCACCGC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGccc                                                       <  2:262017/251‑1 (MQ=14)
                                                                                                                                                                        aaGAGTTCATATCGACGTCTGTGTTTGGCACCTG‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCTCTGGAGAACTGATGGGGGCTGCTCCTAGTACGAGAGGACCGGATTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCg                                                      <  1:379171/251‑1 (MQ=11)
                                                                                                                                                                        aaGAGGTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCg                                                      <  2:478448/251‑1 (MQ=14)
                                                                                                                                                                         agagTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAAGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATTGCACTGCCCgg                                                     >  1:39269/1‑251 (MQ=14)
                                                                                                                                                                                         gcggTGTTTGGCACCGT‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGCCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAAGGGCACTGCCCGGTAGCTAAATGCGGAag                                     <  2:503704/251‑1 (MQ=9)
                                                                                                                                                                                          cggTGTTTGGCCCCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAACGGTATGGCTGTTCGCCCTTTCAAGAGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCAGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTAATGCCCATGGCACTGCCCGGTAGCTAAATGCggc                                       >  2:257463/1‑247 (MQ=11)
                                                                                                                                                                                              gtTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGCGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGAt                                 >  2:217887/1‑250 (MQ=14)
                                                                                                                                                                                              gtTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGAt                                 >  1:304325/1‑250 (MQ=14)
                                                                                                                                                                                              gtTTGGCACCTC‑GATGTCGGCTCATCACAGCCTGGAGCTGAATGT‑GGTCCCAAATGTATTGCTGGTCGCCATTGTAAGTGGTACGCGAGCTGTGTTTAGACGGTCGTGCGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGACCTGAGGGGGGCTGCTCCTCGTACGAGAGGCCCGGAGTGGACGAATCACTCGGT‑TTCGGTTTGTCATGCCAATAGCCCTGCCCGGTAGCTAATTGCGTAAGAGATa                                <  2:312540/251‑1 (MQ=2)
                                                                                                                                                                                               tttGGCACCTC‑GATGTCGGCTCATAACATCATGGAGCTGAA‑GTAGGTCCCCAAGGTATGGCTGTTCGCCTTTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGACGTGGGCGCTGAAGAACTGAGGGGGGCCGCTCCTAGTACGAGAGGGACGGAGTGGACCCATCCCT‑GGTGTTCGGGATGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAACAGATa                                >  2:555922/1‑250 (MQ=11)
                                                                                                                                                                                                    cACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGtt                                                                                  >  1:17536/1‑195 (MQ=17)
                                                                                                                                                                                                         c‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCGCT‑GGTGTTCGGGTTGTCATGCCAAGGGCACTGCCCGGTAGCTAAATGCTGAAGAGATAAGTGCTGaa                       <  2:82826/249‑1 (MQ=9)
                                                                                                                                                                                                              gTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCAt                  <  1:262261/250‑1 (MQ=14)
                                                                                                                                                                                                                cGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTa               <  2:225220/251‑1 (MQ=14)
                                                                                                                                                                                                                 ggCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTaa              <  2:485997/251‑1 (MQ=14)
                                                                                                                                                                                                                 ggCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGATGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTa               >  2:303526/1‑250 (MQ=14)
                                                                                                                                                                                                                    tcatcaCATCCTGGAGCTGAA‑GTAGGTCCCAAGGGTATGGCTGTTCGCCCTTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGATAAATGCGGAGGAGATAAGTGCTGAAAGAATCTAAGc            >  2:477320/1‑250 (MQ=9)
                                                                                                                                                                                                                    tcatcaCATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGCGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGc            >  1:428993/1‑250 (MQ=14)
                                                                                                                                                                                                                     catcaCATCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCGCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCa           <  1:343192/250‑1 (MQ=14)
                                                                                                                                                                                                                          caTCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAAc     <  1:189750/251‑1 (MQ=14)
                                                                                                                                                                                                                           aTCCTGGAGATGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGACCGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTAGCTGCCGGGGGCGCTGGAAACCTGCGGGGGGCTGCTCCTAGTACGAGAGGCCCGGAGGGGACGCCTCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTCGCTAAATGCGGAAGCGATAAGTGCTGACAGCATCTAAGCACGAAACt    >  2:389752/1‑251 (MQ=11)
                                                                                                                                                                                                                            tCCTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGcc                                                                    >  1:567975/1‑186 (MQ=17)
                                                                                                                                                                                                                            gccTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTTAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGTGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGcc                                                                    <  2:567975/185‑1 (MQ=12)
                                                                                                                                                                                                                             ccTGGAGCTGAA‑GTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTg  <  1:511670/251‑1 (MQ=14)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    
CCTGGTCGGACATCAGGAGGTTAGTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTC‑GATGTCGGCTCATCACATCCTGGAGCTGAA‑GTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT‑GGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTG  >  NC_000913/3946003‑3946473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑