Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,292	T→C	D46D (GAT→GAC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,292	0	T	C	98.3%	190.0 / NA	59	D46D (GAT→GAC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (39/19);  minor base A (0/1);  total (39/20)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

TAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACG  >  NC_000913/3927054‑3927530                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               tAATGAATCAAAAGTGCGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGc                                                                                                                                                                                                                                    >  1:541150/1‑251 (MQ=255)       aTCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGa                                                                                                                                                                                                                               >  2:245938/1‑250 (MQ=255)           aaaGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaaaa                                                                                                                                                                                                                            >  2:489392/1‑249 (MQ=255)                  gTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCa                                                                                                                                                                                                                   <  1:135060/251‑1 (MQ=255)                     aGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGGGAAAAAGCGGTGCAGGt                                                                                                                                                                                                                >  1:324137/1‑251 (MQ=255)                                tGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGCCGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGCGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGTCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGAGCAGGTAAAAGTGaaa                                                                                                                                                                                                      >  2:314409/1‑250 (MQ=255)                                 gAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGCCAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGc                                                                                                                                                                                                    >  2:266166/1‑251 (MQ=255)                                     gATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTg                                                                                                                                                                                                >  1:542914/1‑251 (MQ=255)                                      attattGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCACGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGGGCAGGTGAAAGTGAAAGCTCTGc                                                                                                                                                                                               >  2:158097/1‑251 (MQ=255)                                       ttattGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGcc                                                                                                                                                                                              >  1:263684/1‑251 (MQ=255)                                             cATGTGTGTCGGTTTATGTTAATTAATCATAAGCAACACGACGCAGGAGTATAAAAAATGAAAAACGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTATCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGAGGATGGCACGCAGGCCAACTTGTAGGGCTGTGCAAAAGCGGTTCAGGAAAAAGCGAAAGCTATGCCtgctgc                                                                                                                                                                                        >  2:218310/1‑251 (MQ=255)                                              aTGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGCTGcc                                                                                                                                                                                       >  2:250256/1‑251 (MQ=255)                                                  gtgtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGt                                                                                                                                                                                   >  1:302513/1‑251 (MQ=255)                                                          ttttGTTGCTGAATGATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGAACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAACGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTg                                                                                                                                                                           >  1:64547/1‑251 (MQ=255)                                                           tttGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTg                                                                                                                                                                           <  2:263684/250‑1 (MQ=255)                                                             tgttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAACTTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGtt                                                                                                                                                                        >  1:97814/1‑251 (MQ=255)                                                              gatgCTTAATCATAAGCAACAGGACGCAGGAGGATAAAAAATGAAAACAGCTTACATAGCAAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCGGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGTGATGTGAAACAGCGGTGCAGGTAACAGAGAAAGCTCTGCCGGATGCCCAGTTCGAAGTGGttc                                                                                                                                                                       >  2:326199/3‑251 (MQ=255)                                                                 gCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGttcatt                                                                                                                                                                    <  1:583332/251‑1 (MQ=255)                                                                    tAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAAAACGTCTGGGGCTGATCGAAGTCCCGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAGAAAGGGGGGCCGGTAAAGGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCAc                                                                                                                                                                 >  2:179267/1‑251 (MQ=255)                                                                            aGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGacg                                                                                                                                                         >  1:123256/1‑249 (MQ=255)                                                                            aGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAAAGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACACCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGa                                                                                                                                                           >  1:23782/1‑249 (MQ=255)                                                                               aaCAGGACGCCGGAGTATAAAAACCGAGAACCGCTTACATTGCCAAACAACATCCAATTATATTAGTGAAATCTCTCTTTTCTCGTAAACTGGAAGAACCTCTGGGGATGCTCGCAGTCCAGGCGCCGATTCTTCGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCCGCATGATCCCCAGTTCGAAGTGGTTCATACAATGGCGACGTgg                                                                                                                                                      >  2:56983/1‑251 (MQ=255)                                                                                 cAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACACCGTCAAATTAGCTTCGTGAAATCTCACTTTTATCGTCAAATGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaa                                                                                                                                                                                                                              >  2:53081/1‑177 (MQ=255)                                                                                 cAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaa                                                                                                                                                                                                                              <  1:53081/177‑1 (MQ=255)                                                                                        cAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTc                                                                                                                                               >  2:557078/1‑249 (MQ=255)                                                                                        cAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAg                                                                                                                                             <  1:557078/251‑1 (MQ=255)                                                                                         aGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTc                                                                                                                                               >  1:339642/1‑248 (MQ=255)                                                                                           gAGTATAAAAAATGACCACCGCTTACATTTCCAAACAACGTCAAATTATCTTCGTGAACTCTCACTTTTCTCGTCACCTGGAAGCACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATTGCCCGCAGGCCCACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAACGCTCTGCCTGATTCCCACTTCGAAGTGGTTCATTCACTGGCgccttggaaacttcctccc                                                                                                                                          >  2:74274/1‑233 (MQ=255)                                                                                            aGTATAAAAAAGGAAAACCGCTTACATTGCCCAACAACGTCAAAGTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCt                                                                                                                                         <  2:324137/251‑1 (MQ=255)                                                                                                 aaaaaaTGAACACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTAATTCCCTGGCGAAGTGGAAACGTCAGACCTTAggg                                                                                                                                    >  2:58599/1‑251 (MQ=255)                                                                                                 aaaaaaTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAgg                                                                                                                                     <  1:245938/250‑1 (MQ=255)                                                                                                    aaaTGCAAACCGCTTACATTGCCAAACAACGTCACATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGCAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCa                                                                                                                                  >  2:104095/1‑250 (MQ=255)                                                                                                    aaaTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCa                                                                                                                                  >  1:121310/1‑250 (MQ=255)                                                                                                      aTGAAAACCGCTTCCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAg                                                                                                                                                         >  2:431014/1‑225 (MQ=255)                                                                                                      aTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAg                                                                                                                                                         <  1:431014/225‑1 (MQ=255)                                                                                                      aTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAac                                                                                                                                >  1:202513/1‑250 (MQ=255)                                                                                                            aCCGCTTACACTGCCCATCAACGTCAAATTAGCTTCGTGAAATCTCACTTGTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCGTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACtt                                                                                                                         <  2:313297/251‑1 (MQ=255)                                                                                                                   aCATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACGGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGcgcg                                                                                                                  <  1:352903/251‑1 (MQ=255)                                                                                                                    cATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCgg                                                                                                                 >  1:58963/1‑251 (MQ=255)                                                                                                                     aTTGCAAAACACCTTCAAATTAGCTCCGTGAAACCTCACTTTTCTCGTCACCGGGAGGAAGGTCTGGGGTGGATGGAGTTCCGGGGGCGGATTTTAACCCGTGTGGGG‑ATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGc                                                                                                               <  2:18980/251‑1 (MQ=255)                                                                                                                       tGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGc                                                                                                               <  2:27678/250‑1 (MQ=255)                                                                                                                         ccAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGCCTTCAGCGCGGGCGa                                                                                                             >  2:459427/1‑250 (MQ=255)                                                                                                                           aaaCAACGTCAAATTAGCTGCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGTCGAAGTTGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAgg                                                                                                          <  1:495517/251‑1 (MQ=255)                                                                                                                              caacGTCAAATTAGCTGCGTGAAATCTCCCTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCt                                                                                                       <  1:318463/251‑1 (MQ=255)                                                                                                                                    cAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTacac                                                                                                 >  1:497061/1‑251 (MQ=255)                                                                                                                                     aaaTTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCAAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACg                                                                                                >  1:132290/1‑251 (MQ=255)                                                                                                                                        ttAGCTTCGAGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGAGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGCAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGAGGGCGAAGGGCTGTACACGccc                                                                                             >  2:559275/1‑249 (MQ=255)                                                                                                                                                         cACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCAGTGTGGGGGATGGCACGCAGGACACCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGccc                                                                            >  2:344513/1‑251 (MQ=255)                                                                                                                                                               tctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGAAAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCGGTACACGCACATGAAAGCCCTTCGCCCCGATg                                                                       <  1:122543/250‑1 (MQ=255)                                                                                                                                                                          ggAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCtt                                                            <  1:489392/250‑1 (MQ=255)                                                                                                                                                                             agaaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCttt                                                           >  2:121533/1‑248 (MQ=255)                                                                                                                                                                             agaaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCttt                                                           <  1:121533/248‑1 (MQ=255)                                                                                                                                                                               aaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCg                                                      >  1:76307/1‑251 (MQ=255)                                                                                                                                                                                      ggggCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGAACt                                               >  2:588375/1‑251 (MQ=255)                                                                                                                                                                                                  aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg                                   <  1:588375/251‑1 (MQ=255)                                                                                                                                                                                                      cAGGCGCAGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGACAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAccc                               >  2:218157/1‑250 (MQ=255)                                                                                                                                                                                                          cgcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACCACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTgg                           >  2:55850/1‑251 (MQ=255)                                                                                                                                                                                                                                ggATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCg     <  1:548040/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGGGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATGCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCGTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGTGAACGCGTAATGTGCGACg  <  2:539794/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               TAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACG  >  NC_000913/3927054‑3927530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑