Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,926,894 C→T G37E (GGG→GAG)  asnC ← DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,926,8940CT95.9% 136.4 / NA 50G37E (GGG→GAG) asnCDNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/31);  minor base G (0/2);  total (17/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACA  >  NC_000913/3926672‑3927135
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
ccTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAg                                                                                                                                                                                                                       <  1:128730/250‑1 (MQ=255)
        aaaGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCg                                                                                                                                                                                                                <  2:451347/250‑1 (MQ=255)
               gtgttgTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGTCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTATTGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGt                                                                                                                                                                                                        <  2:182075/246‑1 (MQ=255)
                                    tAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGc                                                                                                                                                                                   >  2:114810/1‑251 (MQ=255)
                                                    catcaAGGCTTTCCAGCTTGGCCAGCGCGGAAGGGGAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGATGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACg                                                                                                                                                                    <  1:196319/250‑1 (MQ=255)
                                                        aaGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGATCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATGGCCCATTAATGCTTCCAGGATGCCACGGTCCa                                                                                                                                                               <  1:226826/251‑1 (MQ=255)
                                                             tttCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTg                                                                                                                                                          >  2:251837/1‑251 (MQ=255)
                                                              ttCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTg                                                                                                                                                          <  1:141055/250‑1 (MQ=255)
                                                                ccAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTACTATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCg                                                                                                                                                       >  2:114787/1‑251 (MQ=255)
                                                                   gcgttGCCAGGGCTGAAGGGTAGTCTTTGGCGCTTTTTAATTTATTGCCGAAAAAGCAGCCTACGTCATACCCGACCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAAGGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCAGTAATGCTTCCAGGATGCCACGGTCCAGATGGTCGATc                                                                                                                                                    <  2:422177/248‑1 (MQ=255)
                                                                          cAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAAAATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGTTCCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAAt                                                                                                                                             <  1:264556/251‑1 (MQ=255)
                                                                                      gTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGAt                                                                                                                                 >  2:135060/1‑251 (MQ=255)
                                                                                       tAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGAtt                                                                                                                                >  2:451222/1‑251 (MQ=255)
                                                                                           cTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCt                                                                                                                            >  1:450980/1‑251 (MQ=255)
                                                                                                         aaTATAATGCCGATAAATCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCTCCCCGGTCATGATCACCGCCTGCTTCATTTTCTCTACTCGTCCGTGACTCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGTTCCAGATTGTCGTTCGGATACTTTTCCCTAGTTTTTTCTTATGCGGATtgatg                                                                                                              >  2:122543/1‑251 (MQ=255)
                                                                                                         aaTATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGTTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGGCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATtgatg                                                                                                              <  2:139628/251‑1 (MQ=255)
                                                                                                            ataagcacaaTAAAGCAGCCTACGTCATCACCGAGTGGATTCGGGCTGACATCAATACGCGCCCCGGTAAGGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGatt                                                                                                           <  2:22787/242‑1 (MQ=255)
                                                                                                                  ctgATAAAGCAGCTGACGTCATACCCTAGCTGCTTCGGTCTGACATCAATACGCGCCCCGGTAATGATCCCCGCTTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATGAATGCTTCCAGGATGCCACGGTCCAGTTTGTc                                                                                                                                                        <  2:481021/198‑1 (MQ=255)
                                                                                                                    gATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTAt                                                                                                   >  1:368704/1‑251 (MQ=255)
                                                                                                                       aaaGCAGCCTGCGTAATAACCGAGTGGCTTCGGGCTGACATTAATACCCGGTGCGGTAGCTACCCCCGCGTGCTTCATTTTCGCTACTCGAACGTGAATCGTCTGCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATTCCACGGTAGAGATTGTCGAACAGATAATTTTCCATAGGATTTTCGTATGCGGTTTGATGATACATTCTAttg                                                                                                 <  2:30260/250‑2 (MQ=255)
                                                                                                                        aagcccgcgaggcactaacagggctgctGCGGGCTGTCATCACTGCGCGCCCCGTTAAGTACCTCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGTACTGACGCCAAATTGTTTCGCCAGGTCGGCGTAAGCGGTGCGCTCATTTCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATATGAGTTTCGTATACGGATTGATTATTCATTCTAtttg                                                                                                <  2:439928/228‑2 (MQ=255)
                                                                                                                            ggcCTGCGTCAAAACCGAGCGGCTTCGGGTTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGGTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTGTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCct                                                                                           <  1:50422/250‑1 (MQ=255)
                                                                                                                              ccTACTTCATAACCGAGCTGCTTCGGGTGGACATCAATACGCTCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCcttc                                                                                         <  1:208813/251‑1 (MQ=255)
                                                                                                                                 aCGTCATACCCGAGCTGCTTCGGGTTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCcttct                                                                                        <  1:251837/249‑1 (MQ=255)
                                                                                                                                 aCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCcttct                                                                                        <  1:114787/249‑1 (MQ=255)
                                                                                                                                      ataccctaGCTGCTTCGGGCTGACATCAATCCGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTaa                                                                                 <  1:206711/244‑1 (MQ=255)
                                                                                                                                                 gctTCGGGCTGACATCAATCCGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTGCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCaaaa                                                                       <  1:290633/250‑1 (MQ=255)
                                                                                                                                                    ccGGGCTGCCATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGa                                                                   <  1:36733/250‑1 (MQ=255)
                                                                                                                                                              aTCAATACGCGCTCCGGTATGGACCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTGCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTGTTTAATGAATCAAAAGTGAGTGAGGAttt                                                         <  2:4154/251‑1 (MQ=255)
                                                                                                                                                                cAATGCGCGCCCCGGTAATAATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATGCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCttttt                                                       <  1:173562/251‑1 (MQ=255)
                                                                                                                                                                        gcCCCGGTAGTGATCCCCGCCTGCTTCATTTTCTCTACGCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTGTTTATTGAATg                                               <  2:181712/251‑1 (MQ=255)
                                                                                                                                                                         ccccGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGGTGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGGGAGTTAGGCTTTTTATTGAATg                                               >  1:227153/1‑250 (MQ=255)
                                                                                                                                                                                actGATCCCCCCCTGCTTCATTGTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCAGTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTg                                        <  2:26846/248‑1 (MQ=255)
                                                                                                                                                                                aaTGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGc                                       <  1:66051/251‑1 (MQ=255)
                                                                                                                                                                                    aaccccGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCGCCGGACTGACGCCAAATTTTTTCGCCAGTTCGGCGTAAGCTGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCAtgt                                   <  1:292221/249‑1 (MQ=255)
                                                                                                                                                                                     tCCCCGCCTGCTTAATTTTCTCTACTCGAACGTGACTAGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCAATTAATGCTTCCAGGATGACACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGAAtgtg                                  >  2:352903/1‑251 (MQ=255)
                                                                                                                                                                                             tGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGtt                          >  2:202379/1‑251 (MQ=255)
                                                                                                                                                                                             tGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGtt                          >  2:318463/1‑251 (MQ=255)
                                                                                                                                                                                                          ctACTCGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCtt                <  2:272858/248‑1 (MQ=255)
                                                                                                                                                                                                               cGAACGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATaa        <  2:450980/251‑1 (MQ=255)
                                                                                                                                                                                                                gAACGTGAATCGTCGCCGGCCTGACGCCAAATTGTGTCGCCATTGCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTTTCGATCAGATAATTGTCCATAGGATTTTCtt                                                                                                                           <  1:497575/135‑1 (MQ=255)
                                                                                                                                                                                                                gAACGTGAATCGTCACCGGGCTGCCGCCAAATTGTTTCGCGAGTTCGGCGTAAGCGGTGCGCGCATTGCCCCTTATTGCTTCCAGGATGCCACGGTCCAGATTGTCGATCCGCTAATTTTCCCTAGGATTTTCtt                                                                                                                           >  2:497575/1‑135 (MQ=255)
                                                                                                                                                                                                                  aCGTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGc      >  1:539794/1‑250 (MQ=255)
                                                                                                                                                                                                                    gTGAATCGTCTCCGGACTGACGCCCAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCAc                                                                                                                                                                     >  2:274457/1‑89 (MQ=255)
                                                                                                                                                                                                                    gTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCAc                                                                                                                                                                     <  1:274457/89‑1 (MQ=255)
                                                                                                                                                                                                                    gTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGa                                                                                                                                  <  1:290160/124‑1 (MQ=255)
                                                                                                                                                                                                                    gTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATg                                                                               <  2:380694/175‑1 (MQ=255)
                                                                                                                                                                                                                    gTGAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATg                                                                               >  1:380694/1‑175 (MQ=255)
                                                                                                                                                                                                                    gTGAATCGTCTCCGGAATGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGa                                                                                                                                  >  2:290160/1‑124 (MQ=255)
                                                                                                                                                                                                                      gAATCGTCTCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGGCGGTTTTTGTTGCTTAATCATAAGCAACa  <  2:274183/250‑1 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
ACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACA  >  NC_000913/3926672‑3927135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑