Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,618,836	C→T	V83V (GTC→GTT)	nikR →	DNA‑binding transcriptional repressor NikR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,618,836	0	C	T	98.8%	277.0 / NA	86	V83V (GTC→GTT)	nikR	DNA‑binding transcriptional repressor NikR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (32/52);  minor base G (0/1);  total (32/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTT  >  NC_000913/3618589‑3619078                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   tGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCCCGACCTCTCC‑GTTGcc                                                                                                                                                                                                                                                 >  2:389519/1‑251 (MQ=255)  gCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGcc                                                                                                                                                                                                                                                 <  2:180998/250‑1 (MQ=255)  gCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGCAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGACCACGAAAAACGCGACTTAGCCAGCCGCATTGGCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGcc                                                                                                                                                                                                                                                 >  1:301102/1‑250 (MQ=255)      cGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGTTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACTACCTCTCC‑GTTGCCACGCt                                                                                                                                                                                                                                            <  2:27250/251‑1 (MQ=255)           cACCATCTCGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCAtg                                                                                                                                                                                                                                       <  2:489347/251‑1 (MQ=255)           cACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGATCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGGAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCAGTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCAtg                                                                                                                                                                                                                                       <  1:356839/251‑1 (MQ=255)            aCCATCACGCTTGATGACGATTTACTGGAGACGCTTGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCAtgt                                                                                                                                                                                                                                      <  1:588243/251‑1 (MQ=255)                     cTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACAACGACCTCTCC‑GTTGCCACGCTGCATGACCACATCa                                                                                                                                                                                                                              >  2:177948/1‑250 (MQ=255)                           gatgATTTACTGGAGACGCGGGACAGCCTGGGCCAGCTTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACGTAGCCAGCCGCATTGTCTCCACCCAGCATCAGCACCACGCCCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCacg                                                                                                                                                                                                                        <  2:376083/247‑1 (MQ=255)                               aTTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACgacgac                                                                                                                                                                                                                   <  1:393332/251‑1 (MQ=255)                                 ttACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTg                                                                                                                                                                                                                 >  1:479613/1‑251 (MQ=255)                                   aCTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGt                                                                                                                                                                                                                <  1:480581/250‑1 (MQ=255)                                          aCGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAAt                                                                                                                                                                                                        <  2:83701/251‑1 (MQ=255)                                            gCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCg                                                                                                                                                                                                      <  1:57459/251‑1 (MQ=255)                                              tGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGCCGACTGTCTGGCAATCGcc                                                                                                                                                                                                    >  2:212655/1‑251 (MQ=255)                                                   aGCCTGAGCCAGCGTCGTGGTTATAACAACCGGTCCGAAGCTATCCGCGACATTCTGCGTAGCGCGCGGGCGCAAGAGGCCTCCCATCAGCACGGCACGCAAGGTTACGCGGTGC‑TGTCGTATGTG‑TGTGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCTCCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGtt                                                                                                                                                                                               <  1:284374/251‑1 (MQ=255)                                                            cAGCGTCGTGGTTATAACACCCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGTCCTCTCC‑GTTGCCTCGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGtta                                                                                                                                                                                      <  1:465176/251‑3 (MQ=255)                                                               cgtcgtGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACAt                                                                                                                                                                                   <  1:28154/251‑1 (MQ=255)                                                                    tGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGcc                                                                                                                                                                                                                                                 >  2:540142/1‑184 (MQ=255)                                                                    tGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGcc                                                                                                                                                                                                                                                 <  1:540142/184‑1 (MQ=255)                                                                     ggTTATAACAACCGGTCCGAAGCTATCCGCGGCATCCTGCGTAGCGCTCTGGCGCAAGAGTACACCCAGCAGCACGGCATGCAAGGTTTCGCTGTGC‑GGTCGTATTTG‑TATGAACACAAAAATCGCGACTTAGCCAGCCGCATTGTCTCCCCCCAGCAGCACCACCACGACCTCTGC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTTTTGAAAGGTGACATGGGTGa                                                                                                                                                                             <  2:23908/251‑1 (MQ=255)                                                                      gTTATAACAGCCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCCCGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCTCCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGAc                                                                                                                                                                            <  2:434184/251‑1 (MQ=255)                                                                        tataACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACgg                                                                                                                                                                          >  1:533465/1‑250 (MQ=255)                                                                           aacaacCGTTCCGAAGCTAACCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACGGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTgca                                                                                                                                                                       <  2:75586/251‑1 (MQ=255)                                                                            acaacCGTTCCGAAGCTATCCGCGACACTCGGCGTAGCGCCCGGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAATGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACGTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCGGGAAATCGCAGTGTTGAAAGGTGACATGGGTGACGTgcag                                                                                                                                                                      <  2:283489/251‑1 (MQ=255)                                                                                        aagcaatccgcgacaggtggCGTAGCGCCCGTGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACTACGACTGTCTGGAAATCGCCTTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGAt                                                                                                                                                          <  2:388775/232‑1 (MQ=255)                                                                                             aTCCGCTACATTCTGCGTAGCCCCCTGGCGCGAGAGGCCACCCAGCAGCACGGCACGCAAGGTTGCGCGGTGC‑TGGCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGTAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACg                                                                                                                                                      <  2:19031/250‑1 (MQ=255)                                                                                                cgcgACATTCTGCGTAGCGACCTGGCGCACGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGAGGTGC‑TGTCGTATGTG‑TATGAACACGACAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTAt                                                                                                                                                  >  2:570537/1‑251 (MQ=255)                                                                                                   gACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGGGAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACAAAAAACGCGACTTAGCCAGCCGCATTGTTTCCCCCAAGCATAATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAACTCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGc                                                                                                                                               >  1:304086/1‑251 (MQ=255)                                                                                                        tCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCAGGTGCACATCAACCACGACGACTGTCTGGAAAACGCCGTGTTGAAAGGTGACATGGGTGACTTGCATCATTTTGCCGATGACTTTATCGCCCAg                                                                                                                                           <  2:572551/250‑1 (MQ=255)                                                                                                                    ccTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGc                                                                                                                              >  1:183060/1‑251 (MQ=255)                                                                                                                       ggCGCAAGATGCCACCCAGCCTCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGTCTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCAct                                                                                                                           <  1:22252/251‑2 (MQ=255)                                                                                                                       ggCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCa                                                                                                                             <  1:436906/249‑1 (MQ=255)                                                                                                                        gcgcAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACgg                                                                                                                          <  1:112513/251‑1 (MQ=255)                                                                                                                         cgcAAGAGTCCACCCCGCAGCACGGCACTCAAGGTCTCTCGGTGCCTG‑CGTATGTG‑GATGTACCAGAACAACGCGTCTTAGCCAGCCGCTGTGTCTCCACCCAGCCTCATCACCACGACCTCTCC‑TTGCCCTCGCGGCAGGTGCTCATCAACCGCTACGACTGTCGGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCGTCATTTTGCCTATGACGTTCTCGCCCAGCGCGCCGTGCGGCACggg                                                                                                                         <  2:540183/251‑1 (MQ=255)                                                                                                                            aaGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCAt                                                                                                                      >  1:383911/1‑251 (MQ=255)                                                                                                                            aaGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGCAGCCTGCG‑TATGAACACGAAAAACGCGAATTAACACGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GATGCCACGCTGCATGTGCACATCAACCACGACGAATGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCAGATGACGTTATCGCCCAGCGCGGCGTGCGGGACGGGCAt                                                                                                                      >  1:42489/1‑251 (MQ=255)                                                                                                                                 gCCACCCAGCAGCACGGCACGCAAGGTTTCGCTGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGGCTCCACCCAGCATCATCTCCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGGGCAGCATTTTGGCGATGACGTTATCGCCCAGCGCGGCGTGCTGCACGGGTATTTGCa                                                                                                                 <  2:254523/251‑1 (MQ=255)                                                                                                                                   cACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGt                                                                                                               <  1:363585/251‑1 (MQ=255)                                                                                                                                   cACCCAGCACCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTACCCAGCCGCATTGTCGCCACCCAGCATCAGCGCCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTTTCTGGAAATCGCCGTGTGGAAAGGTGAGATGGGTGACGTGCAGCATTGTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGt                                                                                                               <  1:278620/251‑1 (MQ=255)                                                                                                                                    ccccAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTg                                                                                                              <  1:455943/250‑1 (MQ=255)                                                                                                                                       cagcagCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TAGGAACACGAAAAACGCGTCTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCAAGGGCAGTTGCAGTGCtt                                                                                                           <  2:301102/251‑1 (MQ=255)                                                                                                                                         gcagcaCGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TCTGAACACGAAAAACGCGACTTAGCCAGCCGCCTTGTCTCCAACCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGc                                                                                                         >  1:565797/1‑251 (MQ=255)                                                                                                                                          cagcaCGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGTTGACGATATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGcc                                                                                                        >  1:82844/1‑251 (MQ=255)                                                                                                                                          cagcaCGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGcc                                                                                                        <  1:163104/251‑1 (MQ=255)                                                                                                                                           agcaCGGCACGCAAGGTTTCGCGGTGC‑TGGCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCGCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGATGACTGGCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCTAGGACGTTATCGCCCAGCGCGGCGTTCGGCACGTGCATTTGCAGTGCGTGCCg                                                                                                       <  2:232199/251‑1 (MQ=255)                                                                                                                                              acggcacgCAAGGTTTCGCGGGGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgaag                                                                                                    <  2:399875/251‑1 (MQ=255)                                                                                                                                                  cacgCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgaaggaag                                                                                                <  1:52607/251‑1 (MQ=255)                                                                                                                                                   acgCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGa                                                                                               <  1:115953/251‑1 (MQ=255)                                                                                                                                                    cgCACGGTTTCGCGGTGC‑TGTCGTA‑GTGGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GGTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTTTTGAAAGGTGACAGGGGTGACGTGCAGCATGTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTGGCCGAAGGAAGAt                                                                                              <  2:289047/251‑1 (MQ=255)                                                                                                                                                       aaGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGa                                                                                           <  1:453665/251‑1 (MQ=255)                                                                                                                                                       aaGGTTTCGCGGTGC‑TGTCGTATGCG‑TATGAACACGAAAACCGCGACTTAGCCAGCCGCATTGTCTCCACCCCGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGCCGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTg                                                                                            >  2:266553/1‑250 (MQ=255)                                                                                                                                                              cgcgGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTCGCCTGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCtatt                                                                                    >  2:63554/1‑251 (MQ=255)                                                                                                                                                                  gTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTg                                                                                >  1:313499/1‑251 (MQ=255)                                                                                                                                                                    gC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTggg                                                                              >  1:270420/1‑251 (MQ=255)                                                                                                                                                                             aTGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCCCCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTg                                                                       <  1:188831/250‑1 (MQ=255)                                                                                                                                                                             aTGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTAGCGCCCAGCGCGGCGTGCGGCACGGGCAGTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTg                                                                       <  1:86577/250‑1 (MQ=255)                                                                                                                                                                                 g‑tATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGACGATTGAGTCTATTATTGGGTCCTTTGGTGa                                                                   >  2:161047/1‑250 (MQ=255)                                                                                                                                                                                 g‑tATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGaa                                                                  <  1:265039/251‑1 (MQ=255)                                                                                                                                                                                 g‑tATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGa                                                                   <  1:221754/250‑1 (MQ=255)                                                                                                                                                                                      gacaacGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCCCATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGAt                                                              <  1:9104/247‑1 (MQ=255)                                                                                                                                                                                       aaCACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATc                                                             >  1:240715/1‑251 (MQ=255)                                                                                                                                                                                           cGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATcaaca                                                         <  1:211951/251‑1 (MQ=255)                                                                                                                                                                                             aaaaaCGCGACTTAGCCAGCCGCAGTGTCTCCACCCAGCAGCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGGCTGGAAATGGCCGTGTTGAAAGGGTACATGGGTGACGTGCAGCAGTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGTAAGATTGAGGCTATTATTGTGTCCTGTGGGGAACAATCAACaaa                                                       <  2:562066/251‑1 (MQ=255)                                                                                                                                                                                               aaaCGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAgg                                                     >  1:196350/1‑251 (MQ=255)                                                                                                                                                                                                aaCGCGACTTAGCCAGCCGCAATGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAggg                                                    >  2:354090/1‑251 (MQ=255)                                                                                                                                                                                                    cgACTTAGCCAGCCGCATTGTCTCCCCCCAGCATCATCCCCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGGGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCTGCGGGGGGCACGGGCATTGTCAGTGCTTGCCGAAGGAAGATTGAGTCGATTATTGGGGCCTTTGGTGAACGATCAACAAAGGGCCAc                                                <  2:28918/251‑1 (MQ=255)                                                                                                                                                                                                        ttAGCCAGCAGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGCCATGGGTGCCGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGACGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGc                                            >  2:294427/1‑251 (MQ=255)                                                                                                                                                                                                           gccagccGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCCGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGCGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGcac                                          >  1:287030/1‑250 (MQ=255)                                                                                                                                                                                                             cagccGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCCTTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACAcc                                       >  1:409812/1‑251 (MQ=255)                                                                                                                                                                                                                 cGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑TTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGAt                                   <  1:172790/251‑1 (MQ=255)                                                                                                                                                                                                                 cGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCTCACCTGAt                                   <  1:141580/251‑1 (MQ=255)                                                                                                                                                                                                                   cATTGTCTCCACCCAGCATCATCACCACGACCTCTCCTGT‑GCCTCGCTGCATGTGCTCATCAATCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTTAGTCTATAATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCTCACGTGATTc                                 <  2:62844/251‑1 (MQ=255)                                                                                                                                                                                                                     ttGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCct                               >  1:269208/1‑251 (MQ=255)                                                                                                                                                                                                                             aCCCAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGCTTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAAt                       >  2:399565/1‑251 (MQ=255)                                                                                                                                                                                                                              cccAGCATCATCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACAACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGAGACATGGGTGACGTGCAGCATTTTGCCGATGAAGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGTTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTATAAAt                       >  1:117703/1‑250 (MQ=255)                                                                                                                                                                                                                                   catcatCACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTACTCTCTAAATACCCt                  >  1:331323/1‑250 (MQ=255)                                                                                                                                                                                                                                    atcatcACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTAt                <  1:368510/251‑1 (MQ=255)                                                                                                                                                                                                                                    atcatcACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTAt                >  2:129750/1‑251 (MQ=255)                                                                                                                                                                                                                                    atcatcACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTGGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCct                               <  1:343464/236‑1 (MQ=255)                                                                                                                                                                                                                                    atcatcACCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATAAACCACGACGACTGTCTGGAAATCGCCGTGTTGCAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGAGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGCTTGCGTCTATTATTGGGTCCTTTGGTGCACGATCAACACAGGGCCCATAGCACCCCTGATTCct                               >  2:343464/1‑236 (MQ=255)                                                                                                                                                                                                                                     acatcaCCACGACCTTGCC‑GTTGTCACGCTGCAGGTGCAAATCAACCCCGACGACGGTCTGGAAATCGCCGTGGTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGATTCTATTATTGGGTCCTGTGGTGAACGATCAACAAAGGGCCACTAGCGCACCTGATTCCTCTCTAAATACCCTATc               <  2:186890/250‑1 (MQ=255)                                                                                                                                                                                                                                      catcaCCACGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATc               <  2:196350/250‑1 (MQ=255)                                                                                                                                                                                                                                         caccacGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGa            <  2:10020/250‑1 (MQ=255)                                                                                                                                                                                                                                             ccGACCTCTCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTAGCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCtt       <  2:22032/250‑1 (MQ=255)                                                                                                                                                                                                                                                  ctctCC‑GTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCtt  >  2:289357/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   TGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGC‑TGTCGTATGTG‑TATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCC‑GTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTT  >  NC_000913/3618589‑3619078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑