Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,581,316	G→A	G60E (GGA→GAA)	yhhY →	N‑acetyltransferase YhhY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,581,316	0	G	A	98.8%	276.2 / NA	86	G60E (GGA→GAA)	yhhY	N‑acetyltransferase YhhY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (51/34);  minor base C (1/0);  total (52/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGGAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAA  >  NC_000913/3581072‑3581548                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          gCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGAcgt                                                                                                                                                                                                                                     >  1:428744/1‑251 (MQ=255)  cATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCTATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGAcgt‑c                                                                                                                                                                                                                                   >  1:324411/1‑251 (MQ=255)   aTTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGAcgt‑cg                                                                                                                                                                                                                                  >  1:586689/1‑251 (MQ=255)      tGACCCGCATTTGAAAGTCAATGAGGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCACCCTTCCGATCATATGTGGCAGGAGCGACGCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTg                                                                                                                                                                                                                                <  2:587528/250‑1 (MQ=255)             cATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCAtct                                                                                                                                                                                                                        >  1:581261/1‑251 (MQ=255)              ctttGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGTGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGAAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGCCGG‑CGAGGGCCAtctc                                                                                                                                                                                                                       >  2:325059/2‑251 (MQ=255)                      gTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTg                                                                                                                                                                                                                >  1:135816/1‑250 (MQ=255)                         aaTGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCACCACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGt                                                                                                                                                                                                            >  1:302303/1‑251 (MQ=255)                            gatgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCCGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACAATTGACGTGc                                                                                                                                                                                                          >  1:233487/1‑250 (MQ=255)                             atgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGAGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGCTGAAGACGT‑CGTGGGCAATCTCACCATTGACGTGCa                                                                                                                                                                                                         >  2:162731/1‑250 (MQ=255)                             aagTTGAAATGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTGATACGCCACGCAGAAACACGGGATTACGAGGCCAGCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACGACAGGTGCCTCATCCGTTCGATCATATGTGGCAGGAGCGACGCGCCGATCGTCCCTGCATCAAGCAACTGGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCAGATCTCCATTGACGTGCaa                                                                                                                                                                                                        <  2:95594/249‑1 (MQ=255)                                  gAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATCCGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACGCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAgcg                                                                                                                                                                                                   <  2:202776/251‑1 (MQ=255)                                  gAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAgcg                                                                                                                                                                                                   >  1:190411/1‑251 (MQ=255)                                          cATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCAcgcc                                                                                                                                                                                           <  1:172322/251‑1 (MQ=255)                                                ccccAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTc                                                                                                                                                                                     <  1:316480/251‑1 (MQ=255)                                                 cccAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGGCGCCTGTATTGATGAAGACGT‑CGTGGGCCAGCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCa                                                                                                                                                                                    <  2:520163/251‑1 (MQ=255)                                                   cAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATg                                                                                                                                                                                  >  1:77168/1‑251 (MQ=255)                                                      agagGACAAAGTAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCCCGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTg                                                                                                                                                                               <  1:241797/251‑1 (MQ=255)                                                      agagGACAAAGGAATGAGTGAGAGAGTAATCCGCCACGCAGAAACACGGGATTACAAGGCCAGCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCTACTCGCCGATCGTCCCGGCATCAAGCAACGCTTCTCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTg                                                                                                                                                                               <  1:339764/251‑1 (MQ=255)                                                         ggccaacggAATGAATGAGATCGTAATCCGCCACGCAGAAACACGAGATAACGAGGCCATCAGGCAGCTTCCCGCCCAGCCGGAGGCGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACCCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATAGATCAAGACGT‑CGTGCGCCATCTCACCCTTGACGTGCAACAGCGCCCACGGCGAAGTCATGTTGCCg                                                                                                                                                                            >  2:236470/8‑251 (MQ=255)                                                           accaaGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGCTCGTCCCGGCATCCAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATATCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGc                                                                                                                                                                              >  2:382402/4‑247 (MQ=255)                                                             aaaGGAATGAGTGAGATAGTAATAAGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTAACGCCCAGCCGGAGGTGTATTGCAACCCACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGCTGAAGACGT‑CGTGGGACATCTCACCATTGCCGTGCAACAGCGCCCATGCCGCAGTCATGTTGCAGAttt                                                                                                                                                                        >  2:563323/1‑251 (MQ=255)                                                             aaaGGAATGAGTGAGATAGAAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGAttt                                                                                                                                                                        >  1:201560/1‑251 (MQ=255)                                                               aGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTg                                                                                                                                                                      >  1:344718/1‑251 (MQ=255)                                                                  aaTGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTa                                                                                                                                                                   <  1:475460/251‑1 (MQ=255)                                                                       gtgagATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGCTTTTGGTATCtgt                                                                                                                                                              >  1:5500/1‑251 (MQ=255)                                                                        tgagATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCtgtg                                                                                                                                                             >  1:20383/1‑251 (MQ=255)                                                                        tgagATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGACCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCtgtg                                                                                                                                                             >  2:216459/1‑251 (MQ=255)                                                                         gagaTAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTgtgt                                                                                                                                                            >  1:127583/1‑251 (MQ=255)                                                                           gaTAGTAATCCGCCACGCAGAAACACGGGTTTACGAGGCCATCAGTCAGATTCTCGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTTTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTgtgtag                                                                                                                                                          <  2:375900/251‑3 (MQ=255)                                                                                 aaTACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGCTCGTCCCGGCATCAAGCAACTCGTCGCCTGGATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACtctc                                                                                                                                                    >  2:577472/1‑251 (MQ=255)                                                                                 aaTACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACtctc                                                                                                                                                    >  1:34812/1‑251 (MQ=255)                                                                                 aaTACGCCACGCAGAAACACGGGATAACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGAAACGCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGActct                                                                                                                                                     <  1:212443/250‑1 (MQ=255)                                                                                 aaTACGCCACACAGCAACACGGGATTACGCGGCCATCAGGCAGATTCAAGCCCAGCCGGAGGAGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCCTCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTTCCGATTTTGGTATATGTGTCGACtctc                                                                                                                                                    >  2:546215/1‑251 (MQ=255)                                                                                  aTACGCCACGCAGAAACAAGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGCCGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCg                                                                                                                                                   >  1:501291/1‑251 (MQ=255)                                                                                     cGCCACGCAGAAACACGGGATTACGAGGCCATCCGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCACCTCGTCGCCTGTATTGCTGAGGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTTTCGACTCttgttg                                                                                                                                                >  2:404489/1‑251 (MQ=255)                                                                                         aCGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGCCTCTCGTTGga                                                                                                                                              >  2:1702/1‑249 (MQ=255)                                                                                           gCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCTTTGgaagaa                                                                                                                                          <  2:581261/251‑1 (MQ=255)                                                                                             aGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGCGGTGTATTGCAACACACTACCGGTGCCTCATCCTTCCGCTCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCACGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCtgt                                                                                                                                                              >  1:65071/1‑229 (MQ=255)                                                                                             aGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGGGTATTGCAACACACTACAGTTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGGCCCGGCATCAATCAACGCGGCGCCTGTATTGAGGAAGAGGT‑CGTGGGCCATCTCACCATGGACGTGCAACAGGGCCCACGCCGCAGTCATGTTGCTGATTTTGTGATCtgt                                                                                                                                                              <  2:65071/229‑1 (MQ=255)                                                                                                   ccGGGATGACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACCCTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCgcggcg                                                                                                                                  <  2:235782/250‑1 (MQ=255)                                                                                                     gggATTACGAGTCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTGCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCCCCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTc                                                                                                                                <  2:223337/251‑1 (MQ=255)                                                                                                       gATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGc                                                                                                                              >  1:385175/1‑251 (MQ=255)                                                                                                           acgtggCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCAACAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGAGTTTGGTATCTGTGTCGACGCTCGTTGGAAGAACCGCGGCGGCGCCAg                                                                                                                           <  2:139804/246‑1 (MQ=255)                                                                                                           aCGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAgc                                                                                                                          >  1:174573/1‑251 (MQ=255)                                                                                                             gAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAgcgc                                                                                                                        <  2:270394/251‑1 (MQ=255)                                                                                                                gCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCt                                                                                                                     <  2:302303/251‑1 (MQ=255)                                                                                                                 ccATCAGGCAGATTCGCGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTg                                                                                                                    <  2:62095/251‑1 (MQ=255)                                                                                                                  cATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTTTCTGTGGCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTg                                                                                                                    >  2:496930/1‑250 (MQ=255)                                                                                                                   ataaGGCAGAGTCACGCCCAGCCGGAGGTGTATTGAAACAAACTACAGGTGCCTCATCCTTCCGATCATATATGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAAAAGCGCCCACGCCGCAGTCATGTTGCCGATTTTTGTATCTGTGTCGACTCTCGTTGGACGCACCGCGGCGCCGCCAGCGCCCTgtt                                                                                                                  >  2:144566/4‑249 (MQ=255)                                                                                                                   aTCAGGCAGAGTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAt                                                                                                                  <  1:329784/251‑1 (MQ=255)                                                                                                                     cAGGCAGATTCACGCCCAGCCGGAGGGGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGc                                                                                                                <  1:25131/251‑1 (MQ=255)                                                                                                                       ggCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgg                                                                                                              >  1:487443/1‑250 (MQ=255)                                                                                                                           gATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgag                                                                                                             >  1:91493/1‑248 (MQ=255)                                                                                                                               cACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCCGCGCCCTGATGCGAGAgatgat                                                                                                      >  2:241288/1‑251 (MQ=255)                                                                                                                                aCGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCTATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGAtt                                                                                                     <  2:497889/251‑1 (MQ=255)                                                                                                                                  ccccAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGa                                                                                                   <  2:446157/250‑1 (MQ=255)                                                                                                                                   cccAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGaa                                                                                                  <  1:190357/251‑1 (MQ=255)                                                                                                                                            gggTGTAGTGCAACACACTACAGGTGCTTCATCCTTCCGAGCAAATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAc                                                                                         <  2:176064/250‑1 (MQ=255)                                                                                                                                             ggTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAc                                                                                         >  1:426494/1‑250 (MQ=255)                                                                                                                                                      cAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGc                                                                               >  1:252299/1‑251 (MQ=255)                                                                                                                                                      cAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGc                                                                               >  1:439480/1‑251 (MQ=255)                                                                                                                                                       aacacgatACAGGTGCCTCATCCTTCCGATCATATGTGGCTGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACTT‑CTTGGGCCAGCTCACCATGGTCGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTTTCGGCTCTCGTTGTAAGAACCGCGGCGTCGCCAGCGACCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCg                                                                              <  1:190296/244‑1 (MQ=255)                                                                                                                                                       aaCACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCg                                                                              >  1:386811/1‑251 (MQ=255)                                                                                                                                                        acacacTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCgg                                                                             <  2:127583/251‑1 (MQ=255)                                                                                                                                                          acacTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATTAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCACGCGCCCAGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCggg                                                                            >  1:531792/1‑250 (MQ=255)                                                                                                                                                             cTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGt                                                                           >  1:312215/1‑248 (MQ=255)                                                                                                                                                               aCAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGt                                                                                                                                                                                 >  1:509419/1‑144 (MQ=255)                                                                                                                                                               aCAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGt                                                                                                                                                                                 <  2:509419/144‑1 (MQ=255)                                                                                                                                                               aCAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGG‑CGTGGGCCATCTCACCATTAACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATc                                                                      <  1:540536/251‑1 (MQ=255)                                                                                                                                                                 aGGTGCCTCATCCTTCCGCTCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGAAACTCGTCGCCTGTATTGATGCAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCCACGCCCTGATGCGAGCGATGATTGAAATGAGCGACAACTGGTTGCGGGTAGATCGc                                                                    >  1:219272/1‑251 (MQ=255)                                                                                                                                                                         cATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACt                                                            <  2:586689/251‑1 (MQ=255)                                                                                                                                                                                  gATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGtt                                                   >  1:254383/1‑251 (MQ=255)                                                                                                                                                                                     cATATGTTGCAGGAGCGACCCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGGATCTGTGTCTACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTg                                                 <  1:537096/250‑1 (MQ=255)                                                                                                                                                                                                   gCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTTATGAAGA‑GTGCGTGGGCCATCTCACCATGGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGTTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGATATGATTTAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCgg                                  <  1:234992/251‑1 (MQ=255)                                                                                                                                                                                                         cGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTTGTTGCGGGTAGATCGCATTGAACTAGCCGTGTTTGTCGATAACGCCCCTGCAAt                              <  2:405535/249‑1 (MQ=255)                                                                                                                                                                                                            cgctCGTCCCGGCATCAAGCAACTCGTCGACTGTCTTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGAGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAgg                         >  2:101438/4‑251 (MQ=255)                                                                                                                                                                                                             gATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGACCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCTCCGGCAATTAAggg                        >  2:410957/1‑250 (MQ=255)                                                                                                                                                                                                                 gTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCtat                    >  2:511685/1‑251 (MQ=255)                                                                                                                                                                                                                    ccGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATaa                  >  1:331322/1‑250 (MQ=255)                                                                                                                                                                                                                      ggCATCAAGCAAATCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCATGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATaaca                >  1:211582/1‑248 (MQ=255)                                                                                                                                                                                                                        cATCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCCTTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGTCAATTAAGGTCTATAAAAAAt             >  2:506322/1‑251 (MQ=255)                                                                                                                                                                                                                         aTCAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAAt             >  1:408163/1‑250 (MQ=255)                                                                                                                                                                                                                           cAAGCAACTCGTCGCCTGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGGCTATAAAAAATAc           <  1:458806/250‑1 (MQ=255)                                                                                                                                                                                                                                 acacgtcgCATGTATTGATGAAGACGT‑AGTGGGCCATCTTACCATTGACGTGCAACAGCGCCCAAGCCGCAGACATGTTGAAGATTTTGGTATCTGTGTCGACTCCCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGGGATGATTGAAATGTGCGACAACTGGCTGCGGGTAGATCGCATTGGACTAACCGTGTTTTTCGGTAACGCCCCGGCAATAAAGTTCTATAAAAAATACGGCttgg    >  2:586759/4‑249 (MQ=255)                                                                                                                                                                                                                                    cgtcgCATGTATTGATGAAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGaa  >  1:74610/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          GCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGGAGACGT‑CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAA  >  NC_000913/3581072‑3581548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑