Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	502,232	G→A	T336I (ACC→ATC)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	502,232	0	G	A	100.0%	212.4 / NA	65	T336I (ACC→ATC)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

TGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACG  >  NC_000913/501987‑502478                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       tGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCCTTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAAt                                                                                                                                                                                                                                                   >  1:34664/1‑251 (MQ=255)  gCAAATATCCACTGGGATCTGCTTCTCTTATTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGATTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCCTTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTattg                                                                                                                                                                                                                                                  >  1:194166/1‑248 (MQ=255)  gCAAACATCCACTGGGATCTGCTTCCCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATTTTTCTCCAGTAGGGCGAACAGGACCGGGTTGAGCATAGTCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAAGGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCGGCGCCAGGCTGGCGGCGATGATTAATg                                                                                                                                                                                                                                                  <  2:254502/251‑1 (MQ=255)   cAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGc                                                                                                                                                                                                                                                 >  1:426561/1‑251 (MQ=255)    aaaTATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGcc                                                                                                                                                                                                                                                <  1:43635/251‑1 (MQ=255)          ccACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCCGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGt                                                                                                                                                                                                                                          >  2:304910/1‑251 (MQ=255)                 taTCTGCTTCTCTTCTTCGCTTGCCTCTTCCAACGTCTGCTCTTCCAGCGTTTAGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCCATGCCATTCCCACTCCCGCCAGGATAAACGCGAACTAACCCATCTGCGCCAGGCTGGCGGCGATGATTACTGCCGTACGTTGGGAgg                                                                                                                                                                                                                                   >  2:503700/2‑250 (MQ=255)                   tCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGCGTg                                                                                                                                                                                                                                  >  2:274349/1‑250 (MQ=255)                            tttcttcatttGCCTCTTCCCGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCCCCATTAGTGCGAACAGTACCGGGTTGAGCAAAATCGCCAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATGCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAg                                                                                                                                                                                                                         <  2:48277/241‑1 (MQ=255)                                   gatggcctctctcatcGTCGGCTCTCCCAGCTTGTCGGGCTCCGCAAGATAATTCCCCATTAGTGCGAACTGTACCGGTTTGAGCATAATCGACAGGATCGCCCCTGCCAGTCCCAGGTTTTGTCCGGCCGGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCCATGATTAATGCCGTACGTGGGGAGTTACCAAACAGTCTCACCa                                                                                                                                                                                                                 <  2:34664/236‑1 (MQ=255)                                           ctTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTCCCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTCCCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGaaaa                                                                                                                                                                                                           <  1:563107/249‑1 (MQ=255)                                              ccAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCg                                                                                                                                                                                                      <  1:461859/251‑1 (MQ=255)                                              ccAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCg                                                                                                                                                                                                      <  1:563546/251‑1 (MQ=255)                                                          ctTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACtt                                                                                                                                                                                           >  1:89554/1‑250 (MQ=255)                                                            cccAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTAc                                                                                                                                                                                         <  1:341540/249‑1 (MQ=255)                                                                  gTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAAc                                                                                                                                                                                    >  1:178145/1‑249 (MQ=255)                                                                    ttCGGTCTTCGCAAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCCGTACCCGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGCACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTACTGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTAccccacaga                                                                                                                                                                                 >  2:557065/1‑243 (MQ=255)                                                                     tCGGGCTTCGCCAGATATTTCTCCAGGTGTGCGACCGGTACCGGGTTGAGCATAACCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaa                                                                                                                                                                                <  2:4124/250‑1 (MQ=255)                                                                                  gATATTTCTCCAGTGGTGCAACCAGTACCGGGTTGAGCATAATCGACAGGATCGCTCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATCCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGTCTAACGACTTACCAAACAGAATAATCGCCAGCGTc                                                                                                                                                                  <  1:327897/251‑1 (MQ=255)                                                                                  gATATTTCTCCAGTAGTGCGAACAGTACCGGTTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTc                                                                                                                                                                  <  1:381905/251‑1 (MQ=255)                                                                                    tatTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCCGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGc                                                                                                                                                                >  1:8533/1‑251 (MQ=255)                                                                                     atTTCTCCAGTAGTGCTAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGcc                                                                                                                                                               <  1:371561/251‑1 (MQ=255)                                                                                       tgccccAGTAGTGCGAACAGTACCGGTTTGAGCATAGCCGCCAGGACGGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCTGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACGCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTTGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCa                                                                                                                                                              <  1:368489/246‑1 (MQ=255)                                                                                        tctcCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCCCCAGCGTctcccgc                                                                                                                                                            >  2:14328/1‑245 (MQ=255)                                                                                                attGCGAACATTACCGGGTTGCTCATAATCGCCAGGATCGCCCTTGCCATGCCCCGGTTTTGTCCGGCCTGCGGCAGTAAATTCACTGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCTCCATTCTGCGCCAGGCTGGCGGCGATTATTAATGCCGTACGTTGGGAGTGTCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGTCTTACCAAACATAATAATCGCCGGCGTCGCCTGCACTGCCa                                                                                                                                                     <  1:552095/248‑1 (MQ=255)                                                                                                   gCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCg                                                                                                                                                  <  1:435585/250‑1 (MQ=255)                                                                                                      aaCAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTg                                                                                                                                              >  1:452580/1‑251 (MQ=255)                                                                                                           tACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGa                                                                                                                                          >  1:439531/1‑250 (MQ=255)                                                                                                             ccGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCCGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCCCCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGACTAATCGCCCGCGTCGCCCGCACTCCCAGCCGCTGCCGAATc                                                                                                                                       >  2:257577/1‑251 (MQ=255)                                                                                                                  ttGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGAACGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGACGCCAGCCCTGCCAGCGGTTGCTGAATCAGAAt                                                                                                                                  >  1:353114/1‑251 (MQ=255)                                                                                                                    gAGCATAAGCGACAGGACCGCCCCGGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATCCCCAGTCCCGCCAGGATAAACGCGAACGCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAAtt                                                                                                                                 <  1:284193/250‑1 (MQ=255)                                                                                                                      gCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAAAGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGaaa                                                                                                                                                                                                            <  1:312717/173‑1 (MQ=255)                                                                                                                      gCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGACTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCCATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACATTGGGAGTGACCAAACAGTCGCACCGGGaaa                                                                                                                                                                                                            >  2:312717/1‑173 (MQ=255)                                                                                                                          aaccGACAGGATCGCCCATGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGAt                                                                                                                          <  2:140715/248‑1 (MQ=255)                                                                                                                                    aTCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCCCTGCCAGCGGTTGCTGAATCAGAATTAACGGAACAAacaaca                                                                                                                 >  2:462095/1‑250 (MQ=255)                                                                                                                                      cGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATGAACGGATCAAACAACATc                                                                                                               <  1:143883/250‑1 (MQ=255)                                                                                                                                                    ccgggTTTT‑TCCGGCATGCGCCAGTAAATTCAATGCCATGCCCAGGCCCGCCAGGATAAACGCGATCTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTATGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTGCCAAACAGAATAATCTCCAGCGTCTCCAGCACTTCCAGCGGTTTCTGAATCAGAATGAACGGATCAAACAACATCTCGACGTAGACaaaaa                                                                                               <  2:395660/248‑1 (MQ=255)                                                                                                                                                     cAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACaaaa                                                                                                >  1:3659/1‑250 (MQ=255)                                                                                                                                                      aGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCAAGCGGTTGCTGACTCAGAATTAACGGATCAAACAACATCCCGACGGAGACaaaaa                                                                                               >  1:212265/1‑250 (MQ=255)                                                                                                                                                       ggTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGCTCAAACAACATCCCGACGGAGACAAAAAAc                                                                                             >  2:176096/1‑251 (MQ=255)                                                                                                                                                        gTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAAc                                                                                             >  1:299613/1‑250 (MQ=255)                                                                                                                                                          tttGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGCGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGAGGATGCTGAATCAGAATTAACGGATCAAACAACATCCCGCCGGCGACAAAAAAAAGc                                                                                          >  2:483272/1‑251 (MQ=255)                                                                                                                                                                      gCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGcc                                                                                                                                                      >  2:295619/1‑179 (MQ=255)                                                                                                                                                                      gCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGcc                                                                                                                                                      <  1:295619/179‑1 (MQ=255)                                                                                                                                                                      gCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAAcgcg                                                                              <  2:426561/251‑1 (MQ=255)                                                                                                                                                                        ggCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCCGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTc                                                                            >  1:108912/1‑251 (MQ=255)                                                                                                                                                                                aTTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATg                                                                    <  1:274349/251‑1 (MQ=255)                                                                                                                                                                                   cAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGc                                                                  >  1:50339/1‑250 (MQ=255)                                                                                                                                                                                          aTTCCCAGGCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTGGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATc                                                          <  1:5682/251‑1 (MQ=255)                                                                                                                                                                                               cAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGAGTATCGTggg                                                     >  1:126170/1‑251 (MQ=255)                                                                                                                                                                                                    ccGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGTTCCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCTGTTGCTGAAGCAGat                                                                                                                                   <  2:534876/168‑2 (MQ=255)                                                                                                                                                                                                    ccGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCCCCAGGAAAAATGCGGCTAACGACTTACCAAACCGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGaa                                                                                                                                   >  1:534876/1‑168 (MQ=255)                                                                                                                                                                                                     cGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGGATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCAc                                               >  1:565817/1‑251 (MQ=255)                                                                                                                                                                                                        cAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGt                                            >  1:211512/1‑251 (MQ=255)                                                                                                                                                                                                           gATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGAc                                         >  1:128195/1‑251 (MQ=255)                                                                                                                                                                                                            aTAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGCCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGAAGGAGACAAAAAACAGCACCGCCAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACt                                        >  1:347676/1‑251 (MQ=255)                                                                                                                                                                                                                     aaCTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTc                                  >  1:203059/1‑248 (MQ=255)                                                                                                                                                                                                                         cACCAATCTGCGCCATGCTGGCGGCGATGATTAATGCCGTACGTTGGTAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACATAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGAACAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCTGCGCGCAATGGCAGCGTATTGTGGGCTGCACGGTGACTCAGTTCAGACTCg                           <  1:29303/251‑1 (MQ=255)                                                                                                                                                                                                                          aCCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGt                          <  1:173338/251‑1 (MQ=255)                                                                                                                                                                                                                           ccAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACTCGTCGCGCAATGGCAGCGTATCGTGGGCGGCGCGGTGACTCAGTTCAGACTCGtt                         >  1:2569/1‑251 (MQ=255)                                                                                                                                                                                                                             aaccTGCGCCAGGATGGCGGCGATGATTAAAGCGGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCCGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCa                       <  2:8533/248‑1 (MQ=255)                                                                                                                                                                                                                                        ggCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCTGCTAACGACGTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCg            <  2:299613/251‑1 (MQ=255)                                                                                                                                                                                                                                             gcggcgATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCaaa       <  2:128195/251‑1 (MQ=255)                                                                                                                                                                                                                                               ggcgATGATTACTGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCaa        >  1:420549/1‑248 (MQ=255)                                                                                                                                                                                                                                                 cgATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAAc   >  1:500250/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gatgatTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACg  >  1:372795/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       TGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACG  >  NC_000913/501987‑502478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑