Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	145.7 / NA	46	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/26);  total (20/26)

TGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACG‑CC‑GTGCGTCAGATCCTGCGC  >  NC_000913/3329514‑3329985                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gggTGATATGGCTTTTATACGCGTGGCATCTTATTGCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGTTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTa                                                                                                                                                                                                                                   <  1:361052/250‑1 (MQ=255)    tGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATc                                                                                                                                                                                                                                 >  2:37508/1‑250 (MQ=255)          ggCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTggg                                                                                                                                                                                                                          >  1:295610/1‑250 (MQ=255)              ttcatacgcagtgcttCTTACTACCCCGTTACGAGGTTCAGCCAGGTACGCACCCTCCCCCGGAGTTCGGCCGAAGCGCAATACTGCTAACAGGATGTGGTGACAGCGGACCTGAATCGCTTTATGCGGACGGGAGGCCGTCGACAACGTTCTGAGCCGCTCCCGTGGGCGTTTGTCGTGCAGGATGGATCCAGCTATGCCGGCGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCg                                                                                                                                                                                                                       <  1:237683/235‑1 (MQ=255)                     gcgTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACActg                                                                                                                                                                                                               <  1:218593/251‑1 (MQ=255)                      cgTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACActgc                                                                                                                                                                                                              <  2:463290/251‑1 (MQ=255)                                   taCCCCGTTACGCTGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGACAG‑ACTcc                                                                                                                                                                                                >  1:440868/1‑250 (MQ=255)                                    tccccGTTGCGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGGCGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGTATGTATCCAGCTATGCCGGTGCAATTCTGAAATATTAGGGAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCa                                                                                                                                                                                                <  1:560161/249‑1 (MQ=255)                                         gTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCCGGAGCGGCACACTGCCGCGCCAC‑ACTCAgggtt                                                                                                                                                                                           >  1:218865/1‑247 (MQ=255)                                          ttACGATGTTCAGCCAGGTACGCACCCGCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGTCGTGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTaa                                                                                                                                                                                          <  2:514761/250‑1 (MQ=255)                                                           gTACGCTCCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACGGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACGCAGGTTAACGAACCTGGAACGGTAGt                                                                                                                                                                        <  1:289898/251‑1 (MQ=255)                                                                   ccTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTa                                                                                                                                                                <  1:64497/251‑1 (MQ=255)                                                                                       aaGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCAc                                                                                                                                           >  1:461175/1‑251 (MQ=255)                                                                                          cgcAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGTCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGAt                                                                                                                                        <  1:35581/251‑1 (MQ=255)                                                                                           gcAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGGGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGAtt                                                                                                                                       >  1:164028/1‑250 (MQ=255)                                                                                             aatacggcgaccgtgATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTg                                                                                                                                     <  2:548342/237‑1 (MQ=255)                                                                                                   gCGAAGTGGGTGTGGTGCCATGCGCCCGGAACCGCTTTGCGCGTACGGGATGCCTGCCACAACGTTCTGGTCCGCGCCCGTTGTCGTGTGCCGTGCAGGATGGAGCCAGCGATGCCGGTGCAATTCTGAAATATGAGGTAAAACAGGCGTGGATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGGAGTGGCCAGTAAAC‑AGTCGGCCCCGCTTCACGATCTGCTTAAg                                                                                                                               <  2:32453/251‑1 (MQ=255)                                                                                                    cGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGCGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACACAGGTTAACGAACCTGGGACGGGAGATGCCAGTAACC‑AGTCGGCCCCGCCGCACGAGCTgacta                                                                                                                                 >  2:104392/1‑244 (MQ=255)                                                                                                          ggATGTGGTGCCAGGCGACCGTAACCGCTTTACTCTGACGGGATTCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTGTGCCGGGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGAGCTGCTTAAGATTATg                                                                                                                         <  1:88998/250‑1 (MQ=255)                                                                                                                     cAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCg                                                                                                             <  1:467251/251‑1 (MQ=255)                                                                                                                                   ccctgtACGCTGACTGGATGCCCGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGGGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGTTACTGTAGTTGCCAGTAAAC‑AGGCGGCCCCGGTGCACGATCTGCTTAATATTATGCTGATAAATTCGTACATCATGATCGc                                                                                               <  2:549570/246‑1 (MQ=255)                                                                                                                                             tGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGATGCGCCAG‑ACTCAGGGTAACGCACCTGGAAAGGGAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAATCGGACAACATGATCGCCGATACGGtt                                                                                     >  2:242034/1‑251 (MQ=255)                                                                                                                                             tGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACAATGCTGCGCCCG‑ACTCAGGGTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGACGGCCCCGCCGCACGCTCTGCTTCAGATTATGCTGAAAAAGATGGACAACCTGATCGCCGATACGGtt                                                                                     >  2:481378/1‑251 (MQ=255)                                                                                                                                              gactggATGCCTGCCACAACGTTCTGAGCCGCTCCCGTAGGCTTTTGCCATGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCCCGATCTTCTTAAGTTTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGtt                                                                                     <  2:55917/246‑1 (MQ=255)                                                                                                                                                 gggATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTcc                                                                                 <  1:17412/251‑1 (MQ=255)                                                                                                                                                      gcctgccACAACGTTCTGAGCCGCTCCCGGTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATg                                                                            <  2:122920/251‑1 (MQ=255)                                                                                                                                                       cctgccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGGAAAAATCGGACAACATGATCGCCGCTACGGGTTTCCGcctg                                                                            >  2:476647/1‑247 (MQ=255)                                                                                                                                                          gccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGAt                                                                                                                                        <  2:434513/187‑1 (MQ=255)                                                                                                                                                          gccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAAAACTGCTGCGCCAG‑ACTCAGGGTAACGAACCTGGGACGGTAGTTTCCAGTAAAC‑AGTCGGCCCCGCCGCACGAt                                                                                                                                        >  1:434513/1‑187 (MQ=255)                                                                                                                                                                    tCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGttt                                                                                    <  1:339986/229‑1 (MQ=255)                                                                                                                                                                       gggcagctccctgtGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTATCGAACCTGGTACGTTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCtg                                                           <  2:329717/238‑2 (MQ=255)                                                                                                                                                                           cGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAA‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGGCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATTCGCCCTTCAAt                                                       >  2:127496/1‑251 (MQ=255)                                                                                                                                                                            gCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCAACTGGAGCGGAACCATGCTGCGCCAG‑ACTCAGGTTAACGAAACTTGAACGGGAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGGCGGACAACATGATCGCCCATACGGTTTTCCGCAAGGTAAGCCATGCGCGCTTCAAtg                                                      >  1:284135/1‑251 (MQ=255)                                                                                                                                                                              tCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTAGAGCGGGACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAAC‑CGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCCTGATAGGCCATGCGCGCTTCAAtgtg                                                    >  1:570476/1‑251 (MQ=255)                                                                                                                                                                                            gccggtcAGGATGGAGCCAGCTATGCCGGTGCAATGCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCTGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATTTGCCTGGAACATGGc                                       <  2:467020/244‑1 (MQ=255)                                                                                                                                                                                               gTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTCTCCCCTGGAGCCGGACACCGCTGAGCCAGCAC‑CCGGTTAACGAACCCGGCACGGTAGTTTCCAGTAAACCAG‑CCGCCCCACTGCCCAATCTTGTTAAGATTATGCTGAAAAACTCGGAAAACACGATCGCCTAGACGGTTTTCCCCCTGATAGGCCATGCGCGCTTccaagcgcccggaacctcgcgggg                                   >  2:231117/1‑228 (MQ=255)                                                                                                                                                                                                         gAGCCAGCTATGCTGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGGCGGCGCCGCTGCAAGATCTGCTTAAGATTATGCTGAAAAAGTCGGGCAACATGATCGCCGATACGGTTTTCCGCATGAGGGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGAc                         <  1:74031/251‑1 (MQ=255)                                                                                                                                                                                                              aGCTATGCCGGTGCAATACTGAACTATGCGTTAAAACAGGCGGGAATCACCTGGAGACGAACACTTCTGAGCCAG‑ACTCCGGTTCACGAACCTGGAAAGGAAGTTGCCAGTAAAC‑AGACGGCCCCGCTGGACGCTCTGCTTAAGATTCTGCTGAAAAAGTAGGACAACATGATAGCCGATACGGGTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCCGGAACATGGCGGTCCGGGGCGGGCG‑CC‑gg                  >  2:297944/1‑250 (MQ=255)                                                                                                                                                                                                                 tATGCTGGTGCAATTCTGAAATATGAGTTAAAACCGGCGGGTATCACCTGGAGCGGAACACTGATGCGCAAG‑ACTCAGGGTAACGAAACTGGAACGGTAGGTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGGAAAAGGCGGACAACATGATCGCCGGTACGGTTTTATGCATGATAGGCCATGCCCGATTCAATGGACCTGGAAAAAGGCGGGCCggggcacgcgccgtggg                 >  2:95330/1‑237 (MQ=255)                                                                                                                                                                                                                    gCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACG‑CC‑GTGCGTCa            <  1:53363/251‑1 (MQ=255)                                                                                                                                                                                                                       ggcgCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACG‑CC‑GTGCGTCAGAt         <  2:420678/248‑1 (MQ=255)                                                                                                                                                                                                                       ggTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACG‑CC‑GGGCggccgat         >  1:254010/1‑245 (MQ=255)                                                                                                                                                                                                                        gTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACG‑CC‑GTGCGTCAGATc        <  1:299586/251‑1 (MQ=255)                                                                                                                                                                                                                             aTTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACG‑CC‑GTGCGTCAGATCCTgcg   <  1:37508/251‑1 (MQ=255)                                                                                                                                                                                                                              ttCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCCGCACGGTCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGCTAGGCCATGCGCGCTTCCATGTGCCTGGAACATGGCGGGCCGGGTAGGGCG‑CCCGT‑CGTCAGATCCTgcgc  >  1:197736/1‑251 (MQ=255)                                                                                                                                                                                                                              ttCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAAAGGTAGTTGCCCGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCCATGTGCCTGGAACCTTGCGGGCCGGGTCGGGCGGCC‑G‑GCGTCAGATccctcgc  >  1:525673/1‑246 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       TGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAG‑ACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAC‑AGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACG‑CC‑GTGCGTCAGATCCTGCGC  >  NC_000913/3329514‑3329985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑