Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,157	C→T	Q165Q (CAG→CAA)	cyuA ←	putative L‑cysteine desulfidase CyuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,157	0	C	T	100.0%	211.0 / NA	67	Q165Q (CAG→CAA)	cyuA	putative L‑cysteine desulfidase CyuA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/36);  total (31/36)

GAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCC  >  NC_000913/3255915‑3256393                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             gAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTCGCAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACCCCTGTTGGGTaa                                                                                                                                                                                                                                       >  2:253747/1‑250 (MQ=255)        gagcTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAAGATGCAGCCCCCACTGACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCTGAGACTCTTGCTCGCCCTCTGCCACACGCGCCTGTTGGGTAAacaccaca                                                                                                                                                                                                                               <  2:383061/248‑1 (MQ=255)             ttCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCCCTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCAt                                                                                                                                                                                                                           <  1:285231/250‑1 (MQ=255)             ttCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAAcaccacacca                                                                                                                                                                                                                            <  1:137432/249‑1 (MQ=255)              tCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTGCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATc                                                                                                                                                                                                                          <  1:476747/250‑1 (MQ=255)               cGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGt                                                                                                                                                                                                                        >  1:217604/1‑251 (MQ=255)                gCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGCCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCGGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGAACGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCACCGTg                                                                                                                                                                                                                       <  2:366016/251‑1 (MQ=255)                          cgcgCTCGCTCTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTCCCGCTCAAACATTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCGCTGCCACACACTCCTGTTGGGTAAACCCCACCCCATCGTGCGTCTCGatg                                                                                                                                                                                                             <  2:161789/251‑2 (MQ=255)                             gcTCGCCCTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGCCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATCTGc                                                                                                                                                                                                          >  2:102010/1‑251 (MQ=255)                                cGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACGTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACGTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCGCGCCCGCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGGGCGTCTCGATATGcaca                                                                                                                                                                                                       <  2:258673/251‑1 (MQ=255)                                         tttCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACCATGTTGGt                                                                                                                                                                                               >  1:151250/1‑250 (MQ=255)                                           tCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCGCGATATGCACAATGTTGGTAt                                                                                                                                                                                             <  1:102146/250‑1 (MQ=255)                                              aGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGCATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGccc                                                                                                                                                                                         >  2:128516/1‑251 (MQ=255)                                               gCGTCGCGCCAAAATGCAGCCCCCACTTACCGCTTAAACCTGCCTGCGATAACGCACAATATAGCTTCGCGGAATCGAGAAAAAAGCGGATCGCCGCAAACGGGACATCATTGACGAAATTCAGTATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCGCGCCCTCTGCCACACACGCCTGTTTGGTAAACGCCACACCATCGTGCTGCTCTATATGCACAATATGGGTATGAccg                                                                                                                                                                                        <  2:279402/251‑1 (MQ=255)                                                     cgcCAATATGCATCCCCCACTTACCGCTCAAACCTTCCTGCGATAGCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACGTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacg                                                                                                                                                                                  <  1:34925/251‑1 (MQ=255)                                                        cAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAACCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGGAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATg                                                                                                                                                                               >  1:331448/1‑251 (MQ=255)                                                          atatGCAGCCCCCCCGTTCCGCTTAAACCTTACTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGGGGCAAACGGGACTACACTGACGAACTTCAGGAGCTCAGCCAGCTGCGTTCTTGAAAGCACCGGCAGCGGAGGCTCTGGCTCGGCCTCGGCCACACATGCCTGTTGGTTAAACGCCACCTCATTGTGCTGCTGTATATGCACAGTGTTTGTATGCCCACCTACTATGGt                                                                                                                                                                             <  2:579337/251‑1 (MQ=255)                                                                 gCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacacac                                                                                                                                                                      <  1:443344/251‑1 (MQ=255)                                                                 gCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAAGCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacacac                                                                                                                                                                      <  1:102010/251‑1 (MQ=255)                                                                  cccccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGCAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGGGACACACg                                                                                                                                                                     >  2:469000/1‑251 (MQ=255)                                                                   ccccccTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGc                                                                                                                                                                    <  2:461856/251‑1 (MQ=255)                                                                                tCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCg                                                                                                                                                       >  1:10846/1‑251 (MQ=255)                                                                                  aaaCCTTCCTGCGATAACGCACAATTTAGCTTCGCGGACTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGGAAACACCACACCATCGGGCGTCTCGCTATGCACAATGTTGGTATGCCCGCCGACGCTGGGGACACACGCCCACTTCTCACCGtc                                                                                                                                                     >  2:230528/1‑250 (MQ=255)                                                                                  aaaCCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGtt                                                                                                                                                     <  1:456827/251‑1 (MQ=255)                                                                                    aCCTTCCTGCGATAACGCCCAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTcc                                                                                                                                                   >  1:478568/1‑251 (MQ=255)                                                                                        cccgtcgaaaaCGCACAATTTAGCTTCGCGGAATCGAGAATAAGGCGGATCGCCGCAAACGGGACTTCATGGACGACCTTCAGTACCTCAGCCAGCGCCGGTCTGGAAAGCACCGCCAGCGGAGTCTCTTGCTCGCCCTCTGCCTCACACGCCTGTTGGGTAAACACCACACCATCGTGCGGCTCGATAGGCACAATGTGGGTATGCCCGCCGACGATGGTGACACACGCCCACTCCTCGCCGGTCCAg                                                                                                                                                 <  2:333725/240‑1 (MQ=255)                                                                                              gATAACGCACAATTTAGCTTCGCGGTATCGAGAATACAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCCGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGCCGATGGTGACACCCCCCCCCTTCTCACCGTTCCAGCCTTGGgc                                                                                                                                         >  2:354405/1‑251 (MQ=255)                                                                                                     cacaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTg                                                                                                                                    >  1:48056/1‑249 (MQ=255)                                                                                                      acaATTTAGCTTCGCGGAATCGCGAATAAAGCGGCTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCCGACTTTGGCGCGTgagg                                                                                                                                 >  2:114755/1‑250 (MQ=255)                                                                                                         aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCGCAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGagaaga                                                                                                                              <  1:579435/251‑1 (MQ=255)                                                                                                            tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCCGACTTTGGCGCGTGAGAAGAgg                                                                                                                            >  1:246678/1‑250 (MQ=255)                                                                                                              gCTTCGGGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCGCACCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACCCCACACCAGCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTGGGCGCGTGATAAGAGGAtt                                                                                                                         <  2:386323/251‑1 (MQ=255)                                                                                                                 tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTc                                                                                                                       >  1:22722/1‑250 (MQ=255)                                                                                                                  cgcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGAGCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCGTGCTCGCCCTGTGCCACACACGCCGGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCTCGCTGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCAt                                                                                                                     <  1:581683/251‑1 (MQ=255)                                                                                                                     ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGc                                                                                                                  >  2:182870/1‑251 (MQ=255)                                                                                                                      gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGc                                                                                                                  >  1:535776/1‑250 (MQ=255)                                                                                                                       aaTCGAGAATAAAACGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGCTGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGACGAGGATTTCATCGCa                                                                                                                 >  2:557347/1‑250 (MQ=255)                                                                                                                        aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGCGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGAACACTGTTGGTATGCCCGCAGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGcc                                                                                                                 >  2:234893/1‑248 (MQ=255)                                                                                                                          cGAGAATAAAGCGTACCGCCGCAAACGGGACTTCATGTACGAACGTCAGGTTCTCAGCCAGCGTCGGGCTGGAAAGCACCGTCAGCGGATACTCTTGCTCTCCCTCGGCCACACACGCCTGTTGGGTAAACACCACCCCATCGGGCGCCGCGAGATGCACAAGGTTGGTATGCCCGCCGACGATGGTGACACACGCCCTTTTCTCATCGATCCATACTTTGGCGCGTGAGAAGAGGAGTTCATCGCAAGGt                                                                                                             <  2:572907/251‑1 (MQ=255)                                                                                                                           gagaATAAAGCGGATCGCCGCAAACGGGCCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTAGTTCTGGAAAGCAACGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGATCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCACAAGGtt                                                                                                            >  2:304154/1‑251 (MQ=255)                                                                                                                             gaataagacggacctccgcAAACGGGACTTCAGTTACGAACCTCAGGATCTCAGCCAGCGTCGGTCTGGAAAGCACCGTCAGCTGAGACTCGTGCTCTCCCTCTGCCTCTCACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATTCGCAGTGTCGGTATGCCCGCCGACGATTGGGACACGCGCCCGCGTCTCACCTTTCCAGACTGTGGCGCTTGATAAGAGGAATTCATCGCAAGGTTcc                                                                                                          <  2:567481/236‑1 (MQ=255)                                                                                                                              aaTAAAGCGGATCGCCGCAAACGGGACTGCATTGACGAACTTCAGGATCTCGGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTGGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGTTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCt                                                                                                         <  2:65016/251‑1 (MQ=255)                                                                                                                                   aGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGCTATGCACAATGTTGGTATGCCCCCCGACGATGGTGACACCCTCCCACTTCTCACCTTTCCAGCATTGGGCGCGTGAGAAGAGGATTTACTCGAAAGGTTCCTggttc                                                                                                    >  2:388248/1‑248 (MQ=255)                                                                                                                                    gCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCATCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTCTGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCCTCGCAAGGTTCCTGGATCt                                                                                                   >  1:282551/1‑251 (MQ=255)                                                                                                                                     cGGATCGCCGCAAACGGGACTTCATTGACGAACTGCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACTCCAGCGTGCGTCGCGATATGCACAATGTGGGTATGCCCGCCGACGATCGTGACACACGCCCACTTCTCACCGTTCCAGACGTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCtt                                                                                                  <  1:351149/251‑1 (MQ=255)                                                                                                                                      ggATCGCCGCAAACGTGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGGCGTTCTGGAAAGCACCGGCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTa                                                                                                 <  1:234893/251‑1 (MQ=255)                                                                                                                                      ggATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCGTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTa                                                                                                 <  1:561975/251‑1 (MQ=255)                                                                                                                                          cgccgcAAACGGGACGTCATTGACGAACCTCAGGATCTCAGCCAGCGGCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTGTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACg                                                                                              <  1:361578/250‑1 (MQ=255)                                                                                                                                                                aCGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTACACACCACACCATCGTGCGTCTCGATATGCACAATGTTgg                                                                                                                                                                                                >  1:282229/1‑130 (MQ=255)                                                                                                                                                                aCGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACTCCACACCATCGTGCGTCTCGATATGCACAATGTTgg                                                                                                                                                                                                <  2:282229/130‑1 (MQ=255)                                                                                                                                                                    aCTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTGGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAGGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCt                                                                   <  1:222151/251‑1 (MQ=255)                                                                                                                                                                         aGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACAACACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACAGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGc                                                              >  1:276114/1‑251 (MQ=255)                                                                                                                                                                               ccagccagcGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGc                                                        <  1:171648/250‑1 (MQ=255)                                                                                                                                                                                 agccagcGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGcg                                                       >  1:486620/1‑249 (MQ=255)                                                                                                                                                                                 agccagcGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCa                                                       >  1:110693/1‑250 (MQ=255)                                                                                                                                                                                  cccagcGTCGTTCTGGAAAGCCGCGTCCGCGGAGACCCTTCGTCGCCCTCTGCTACACACGCCTGTTGGGTAAACCCCACCCCAGCGTGCGTCTCGATATGCACAATGTGGGTATGCCCGCCGACGATGGGGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCGTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAAt                                                     <  1:262146/250‑1 (MQ=255)                                                                                                                                                                                                ggAAAGCCCCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTg                                       <  1:280580/251‑1 (MQ=255)                                                                                                                                                                                                 gAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGc                                      >  1:450814/1‑251 (MQ=255)                                                                                                                                                                                                    aGCACCGCCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCGGTTGGGTAACCCCCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGGGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTc                                   <  2:217604/251‑1 (MQ=255)                                                                                                                                                                                                       ccctgcAGCGGAGCCTCTTGCTCGCCCTCTGCCACACCCGCCTTTTGGGTAAACACCACACCATCGTGCTTCTCGTTATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCttt                                <  2:168754/246‑1 (MQ=255)                                                                                                                                                                                                         cGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTc                               >  1:42579/1‑250 (MQ=255)                                                                                                                                                                                                                       acacgccgcctttccgaccccccGCCTGTTGGGTAAACACCCCACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGCCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGcc                  <  2:178427/227‑1 (MQ=255)                                                                                                                                                                                                                              gCCCTCCGCCCCACCCGCCTGTTGGGAAAACGCCCCCCCAGCGTGCGTCTCGATATGCACAATGTTGGTAGGCCCGGCGACGAAGGTGACACACGCCCACGTCTCACCGTTCCAGACTTTGGCTCGTGAGAAGAGGTTTTCATCGCAAGGTTCTTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCGGCGCTGTTGCGTCTTTCAGCTCTTCCAGCCCGGCGtt           <  2:349793/249‑1 (MQ=255)                                                                                                                                                                                                                               cccTCTGCCACACTCGCCTGTTGGTTAAACACCACCCCACCGGGCGTCCCGATATGCACAATGTTGGTTTGCCCGCCGACGAGGGTAACTCCCGCCCACTTCTCCCCGTTCCAGACTTGGGCGCTTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCGCGGCGTTggg        <  2:120056/251‑2 (MQ=255)                                                                                                                                                                                                                                  tctGCCACAAACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCAtt     >  2:208951/1‑251 (MQ=255)                                                                                                                                                                                                                                      ccACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTcc  >  1:508685/1‑250 (MQ=255)                                                                                                                                                                                                                                           cacGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGcc                  >  1:178427/1‑229 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             GAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCC  >  NC_000913/3255915‑3256393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑