Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,176,625 G→A S70F (TCC→TTC)  cpdA ← cAMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,176,6250GA98.2% 371.9 / ‑5.5 110S70F (TCC→TTC) cpdAcAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (43/65);  minor base C (2/0);  total (45/65)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAA  >  NC_000913/3176381‑3176871
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
cAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCGTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGAt                                                                                                                                                                                                                                                  <  1:541900/251‑1 (MQ=255)
 aGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATc                                                                                                                                                                                                                                                 <  1:28756/251‑1 (MQ=255)
     tACGTTCCAGCCTCTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTg                                                                                                                                                                                                                                              <  1:15726/250‑1 (MQ=255)
      aCGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTgcg                                                                                                                                                                                                                                            <  1:206131/251‑1 (MQ=255)
      aCGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACAAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGCAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGACAACTTCCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGCATTGATCCTgcg                                                                                                                                                                                                                                            >  2:464646/1‑251 (MQ=255)
         ttCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCCCCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGAAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGCTAGTCCGCCAAGAATTGATCCTGCGCt                                                                                                                                                                                                                                          >  1:376921/1‑250 (MQ=255)
              gCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCa                                                                                                                                                                                                                                    <  1:356859/251‑1 (MQ=255)
                    cAAGCTGAAACTCGCTCAGCTCTCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTc                                                                                                                                                                                                                              <  1:68673/251‑1 (MQ=255)
                       gCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTcgcg                                                                                                                                                                                                                           <  1:511666/251‑1 (MQ=255)
                       gCTGAAACTCGCTCAGCTCACAGTGCGGCACGCCAAACACCTGGCTATCCAGCTTCAGGATTTGCCATTGCACCCCAATAAACACGCGCTTCGCCGGGGAGATCCCCGCATCCTGTAACGCGCTGTACATAGCGGGCTGGAAATCGAGGATGCCCGGCAGCCCGCAGCAGGGCGCCCGTAAAATTGCGATGCCTTCAGCGAAATGATGATAGGCCGAAGAGCATTGATCCTGCGCTACATCACCTGTcgcg                                                                                                                                                                                                                           >  2:251589/1‑251 (MQ=255)
                                cGCTCAGCTCGCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCGGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAgg                                                                                                                                                                                                                  <  1:40622/251‑1 (MQ=255)
                                 gctcagctcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTTATCCTGCGCTAAATCTCCTGTCGCGACAATCAGGt                                                                                                                                                                                                                 <  2:96683/251‑1 (MQ=255)
                                  ctcagctcaCCGTGCGGCACGACAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTc                                                                                                                                                                                                                >  1:430798/1‑251 (MQ=255)
                                     agctcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGa                                                                                                                                                                                                              >  2:140532/1‑250 (MQ=255)
                                      gctcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAAt                                                                                                                                                                                                            >  1:41734/1‑251 (MQ=255)
                                         caCCGTGCGGCCCGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACCCGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAAtt                                                                                                                                                                                                           <  2:163100/249‑1 (MQ=255)
                                         caCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTc                                                                                                                                                                                                          <  2:430613/250‑1 (MQ=255)
                                         caCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCg                                                                                                                                                                                                         >  1:87182/1‑251 (MQ=255)
                                         caCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCg                                                                                                                                                                                                         >  2:239834/1‑251 (MQ=255)
                                             gttcGGCACGCCAAGCACCTGGCTATCCATCAACAGGATTTGCCATTGCTCCTCAATAAACACGCTCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTTTGCATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTTAGCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCt                                                                                                                                                                                                     <  1:563410/248‑1 (MQ=255)
                                                 ggCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGgtg                                                                                                                                                                                                 >  1:586149/1‑251 (MQ=255)
                                                   cACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGtgtg                                                                                                                                                                                               <  1:326489/251‑1 (MQ=255)
                                                      gCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGcc                                                                                                                                                                                            <  2:197275/251‑1 (MQ=255)
                                                           accccTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAACCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTTCGATGCCTTCAGCGAAATGCTTATAGGCCGCAGTGAATTGATCCTGCGCTATATCTCCGGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATc                                                                                                                                                                                       <  1:257034/248‑1 (MQ=255)
                                                           acacCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATc                                                                                                                                                                                       >  1:490453/1‑251 (MQ=255)
                                                           acacCTGGATATCCAGCAACAGGATGTTCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTCCATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGTCGCAGGGCGCACGAAAACTTGCGATGCCTGCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAAGCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATc                                                                                                                                                                                       <  2:147868/251‑1 (MQ=255)
                                                                 ggCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCt                                                                                                                                                                                   <  2:253400/249‑1 (MQ=255)
                                                                 ggCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCAGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTcc                                                                                                                                                                                 >  2:195930/1‑251 (MQ=255)
                                                                 agCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACCCGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTTCTGGTGTGACCGAATCGCCt                                                                                                                                                                                   <  2:585498/248‑1 (MQ=255)
                                                                   cTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTcc                                                                                                                                                                                 >  1:401848/1‑249 (MQ=255)
                                                                     aTCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGc                                                                                                                                                                              >  1:406163/1‑250 (MQ=255)
                                                                       ccAGCACCAGGATTTGCCATTGCTCACCAATACACACGCGCTTCGCCGGGAAGATACCCGCATCCTGTAGCGCGCTGTACATCGCTGGCTGGAAATCGAGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGAGGCCTTCAGCGAAATGCTGATAGGTCGCAGAGAATTGATCCTGCGCTAACTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCAGAATCGCCTCCAGCAcc                                                                                                                                                                           >  1:377235/1‑251 (MQ=255)
                                                                       ccAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAAGTGAGCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCTAATCGCCTCCAGCAcc                                                                                                                                                                           <  2:578687/251‑1 (MQ=255)
                                                                       ccAGCAACAGGAGTTGCCAGTGCACACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCGGTAACGCGCTGTACATCGCGGGCTGGAAAGCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGAGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCAcc                                                                                                                                                                           <  2:152980/251‑1 (MQ=255)
                                                                            aaCAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTg                                                                                                                                                                      >  2:284863/1‑251 (MQ=255)
                                                                             aCAGGATTTGCCATTGCCCACCAAAAAACACGCGCTTCGCCGGGGAGATCCCCGCATCCTGTAACGCGCTGTCCATCGCGGGCTGGAAATCGTGTTGGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCGTCATCGAAATGCTGATAGGCCTCAGAGAATTGATCCTGCGCTAAATCACCTGTCTCGACAAACAGGCCGAATTCGTGCTGGTGGGTCCGAATCGCCTCCAGCACCGCCTgg                                                                                                                                                                     <  2:586149/251‑1 (MQ=255)
                                                                                ggATTTGCCATTGCACACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTaa                                                                                                                                                                  <  1:400023/251‑1 (MQ=255)
                                                                                 gATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAAc                                                                                                                                                                 <  2:220450/251‑1 (MQ=255)
                                                                                 gATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAAc                                                                                                                                                                 >  2:131254/1‑251 (MQ=255)
                                                                                      gCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACtctc                                                                                                                                                             <  1:239834/250‑1 (MQ=255)
                                                                                      gCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCATCCAGCACCGCCTGGTAACtctc                                                                                                                                                             <  1:488425/250‑1 (MQ=255)
                                                                                            gCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCGGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGgtg                                                                                                                                                      <  2:457018/251‑1 (MQ=255)
                                                                                             cTCACCAATACACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGgtgt                                                                                                                                                     >  2:236433/1‑251 (MQ=255)
                                                                                               cACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGttt                                                                                                                                                   <  2:421282/251‑1 (MQ=255)
                                                                                                   aaTAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTAccc                                                                                                                                               >  1:292283/1‑251 (MQ=255)
                                                                                                     tAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTCACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGCCGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGCATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCt                                                                                                                                             >  2:268605/1‑251 (MQ=255)
                                                                                                      aaaCACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCGCCAGCACCGCCTGGTAACTCGCCCAGGTGTTTACCCCt                                                                                                                                             <  2:554923/250‑1 (MQ=255)
                                                                                                        acacGCGCTTCGCAGGGGAGCAACCCGCATTCTATAACGCGCTGTAAATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCAAGACGCAGGGCGCACGCAAACTCGCGATGCCTTCAGCGAACTGCTGATAGGACGCAGAGAATTGATCATGCGCTAAATCACCTGTCGCGACACACCGGTCGCATTCGAGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTaa                                                                                                                                           >  2:564413/1‑250 (MQ=255)
                                                                                                             cgcTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGc                                                                                                                                     >  1:269503/1‑251 (MQ=255)
                                                                                                                  cGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCgg                                                                                                                                >  1:523718/1‑250 (MQ=255)
                                                                                                                  cGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGt                                                                                                                                <  2:87182/251‑1 (MQ=255)
                                                                                                                  cGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACATGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGt                                                                                                                                >  1:354507/1‑251 (MQ=255)
                                                                                                                       gggAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCttt                                                                                                                           <  1:284863/251‑1 (MQ=255)
                                                                                                                          agaTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                                                        >  1:171593/1‑251 (MQ=255)
                                                                                                                            aTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCGGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGTCTGGGAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGc                                                                                                                      <  1:84754/251‑1 (MQ=255)
                                                                                                                               cccGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCaaa                                                                                                                   <  1:114736/251‑1 (MQ=255)
                                                                                                                                ccGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAAc                                                                                                                  >  1:703/1‑251 (MQ=255)
                                                                                                                                ccGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGAAGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAAc                                                                                                                  >  2:325171/1‑251 (MQ=255)
                                                                                                                                   cATCCGGTAACGCGCTGGACAGCGCGGGCTGGAAAGCGTTGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACGTGCGATGCCTGCATCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCATGTCGAATTCGTGCTGTTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCGAGGGGTTTACCCCTAACAGCGCTTCGTGCTGTTGTGCAAACAgg                                                                                                               <  2:34826/251‑1 (MQ=255)
                                                                                                                                    aTCCTGTAACGCGCTGTCCATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGAACGAAACCTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAACTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACACCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGt                                                                                                              >  1:416711/1‑251 (MQ=255)
                                                                                                                                         gTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCCCCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAgt                                                                                                          <  1:249346/250‑1 (MQ=255)
                                                                                                                                              gcgcTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGAGGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCGCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                                                    <  1:210980/251‑1 (MQ=255)
                                                                                                                                               cgcTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                                                    >  1:460343/1‑250 (MQ=255)
                                                                                                                                                 cTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCCGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTACATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGACTCGCCTCCAGCAACGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAAt                                                                                                 >  1:239723/1‑251 (MQ=255)
                                                                                                                                                       aTCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCGGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCTCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGTCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTaa                                                                                           <  1:209463/251‑1 (MQ=255)
                                                                                                                                                           cgGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATc                                                                                       <  1:5950/251‑1 (MQ=255)
                                                                                                                                                                 ggAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGAct                                                                                 <  1:472527/251‑1 (MQ=255)
                                                                                                                                                                 ggAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGAc                                                                                  <  2:406163/250‑1 (MQ=255)
                                                                                                                                                                    aaTCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTg                                                                              <  2:446061/251‑1 (MQ=255)
                                                                                                                                                                          ggTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTGGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCACCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTc                                                                         <  2:41734/250‑1 (MQ=255)
                                                                                                                                                                           gttacccGGCAGACAGACGCAGGGCGCACGAATACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCATGCGCTAAATCACCTGTCGCGACAATCCGGTCGACTTCGTGCTGGTGTGTCCGCATCGCCTCCAGCACCGCCTGGCAACTCTCCCAGGTGTTTACCCCTACCAGGGCTTCGTGCTTTTGTGCCAACAGGTGAGTGTCTGTAATTTGTAAACTCCTGTCTCTGGCCTccc                                                                       >  2:134769/5‑249 (MQ=255)
                                                                                                                                                                               cccGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGGCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagc                                                                   <  2:7393/251‑1 (MQ=255)
                                                                                                                                                                                ccGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAAGCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCGCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagcc                                                                  <  2:29566/251‑1 (MQ=255)
                                                                                                                                                                                         aGACGCAGGGCGCACGAAAACTTGCGATGCATTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGATAAATCAACTGTCGCGACAAGCAGGTCGAATTCGGGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGACAGAGGAAgg                                                         <  2:703/251‑1 (MQ=255)
                                                                                                                                                                                          gACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGCGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAggg                                                        >  2:18844/1‑251 (MQ=255)
                                                                                                                                                                                            cGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCAGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGtt                                                      <  2:275216/251‑1 (MQ=255)
                                                                                                                                                                                                  gcgcACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAgg                                                <  2:119133/251‑1 (MQ=255)
                                                                                                                                                                                                  gcgcACGAAAACGTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAgg                                                <  1:268605/251‑1 (MQ=255)
                                                                                                                                                                                                         aaaaCTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAACCACCTGTCGCGACAATCAGGTCTAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGGCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTc                                           <  2:172248/249‑1 (MQ=255)
                                                                                                                                                                                                           aaCTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCaaa                                       <  1:207594/251‑1 (MQ=255)
                                                                                                                                                                                                               ggCGATGCCGTCATCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCTCTAAATCACCTTTCGCGACAATCAGGTCGAATTCGTTCTGGTGTGGCCGAATCGCCTCCAGCTCCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGTGCCGCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTGTTGCTTCGCCAGCGAGAAAAAGGTTGAACAGGCTTTCCAAATg                                     <  2:240547/248‑1 (MQ=255)
                                                                                                                                                                                                                gCGATGGCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGGTAAATCACCTTTCGCGACAAGCAGGTCGAAGTCGTGCTGGTGTATCCGAATCGCCTCCAGCACCGCCGGGTATCTCTCCCAGGTGTTTACTCCTAACAGGGCTGCGTGCTTTTGTGCAGACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGgtg                                  <  1:109134/251‑1 (MQ=255)
                                                                                                                                                                                                                gCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTCACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAACTGgtg                                  >  2:411089/1‑251 (MQ=255)
                                                                                                                                                                                                                gCGAAGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGgtg                                  <  1:325171/251‑1 (MQ=255)
                                                                                                                                                                                                                 cGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTTGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGgtgt                                 <  1:61372/251‑1 (MQ=255)
                                                                                                                                                                                                                     gccgtCAGCGAAATGCTGATAGGCCGCAGAGAATTGAGCCGGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCtt                             <  2:351742/247‑1 (MQ=255)
                                                                                                                                                                                                                     gCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCtt                             >  2:289904/1‑251 (MQ=255)
                                                                                                                                                                                                                         tCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTTCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAg                           <  1:446241/249‑1 (MQ=255)
                                                                                                                                                                                                                         tCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAAGCAGGTCGAATGCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGTTAACTCTCCCAGGTGTTTACCCCTAACAGGGCGTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAgg                          <  2:76231/250‑1 (MQ=255)
                                                                                                                                                                                                                          cAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGtt                        >  1:421245/1‑251 (MQ=255)
                                                                                                                                                                                                                               aaaTGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTCACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTCGGTTTCacg                   >  2:185568/1‑251 (MQ=255)
                                                                                                                                                                                                                                 aTGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAAtt                                                                                                <  2:13833/172‑1 (MQ=255)
                                                                                                                                                                                                                                 aTGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAAtt                                                                                                >  1:13833/1‑172 (MQ=255)
                                                                                                                                                                                                                                    cTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCAGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTACACGACGCt              >  2:480438/1‑251 (MQ=255)
                                                                                                                                                                                                                                     tGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCt              >  1:465739/1‑250 (MQ=255)
                                                                                                                                                                                                                                     tGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCt              >  1:18310/1‑250 (MQ=255)
                                                                                                                                                                                                                                      gATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCt              <  1:66120/249‑1 (MQ=255)
                                                                                                                                                                                                                                              gCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCgg    >  2:19503/1‑251 (MQ=255)
                                                                                                                                                                                                                                              gCAGAGAATTGATCCTGCGCAAAATTCCCTGTCCCGACAATAAGGCCGAATTGGTGCTGGGGTGGCCGTATCGCCTCCATCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCgg    <  2:416711/251‑1 (MQ=255)
                                                                                                                                                                                                                                                agagAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAAGTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGaa  <  1:236433/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
CAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAA  >  NC_000913/3176381‑3176871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑