| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,150,316 | G→A | E219K (GAG→AAG) | yghA → | NADP(+)‑dependent aldehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,150,316 | 0 | G | A | 94.6% | 221.2 / NA | 74 | E219K (GAG→AAG) | yghA | NADP(+)‑dependent aldehyde reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (41/29); minor base C (4/0); total (45/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCC > NC_000913/3150083‑3150555 | ccATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTg > 1:302181/1‑251 (MQ=255) cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTg > 1:177776/1‑250 (MQ=255) ttCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAat > 1:363731/1‑251 (MQ=255) ccGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGTCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAata < 1:258460/250‑1 (MQ=255) cGGATATTGCAGACCTCCCCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGAATTCGGGTGAATAtt > 2:295127/1‑251 (MQ=255) cGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATAtt < 1:565550/251‑1 (MQ=255) atTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTcgc < 2:177776/251‑1 (MQ=255) gccgACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTTGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGACGCAAGGAATTCGGGCGAATATTGTCGCGcc > 2:449375/4‑251 (MQ=255) cACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGccc < 1:33743/249‑1 (MQ=255) aGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTg > 2:454184/1‑251 (MQ=255) gCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGATCTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTgg > 1:479587/1‑251 (MQ=255) cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGa < 1:428396/251‑1 (MQ=255) cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGa < 1:485302/251‑1 (MQ=255) gAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGAc > 1:299971/1‑251 (MQ=255) gAACAGTTTCAAAAGACCTTTGCCATTAACGTTTGCGCGCTGTGCTTGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTTCAAGTCTCATCTCCACTCCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGGCTATGCGGCTACGAAGGCGGCGAGTCTGAACTACAGCCGTGGCTTGGCAGAACAGGGCGCGAAGAAAGGGATTCGGGTGAATATGTTCGCGCCAGGCCCGATCTGGAc < 2:515716/251‑1 (MQ=255) aaCAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGAc > 2:578231/1‑250 (MQ=255) aaCAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGATCTGTCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGAGCAAGTATCATCACCAATTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACGATGCGGCTACGCAGACGGCGATTCTGAACTACAGCCGTGTCTTGGCAAAACAGGTCGCGAGGAAAGATATTCGGGTGCATATTGTCGCGCCCGTCCCGACCTggcac > 2:308861/1‑248 (MQ=255) cAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGc > 1:479694/1‑251 (MQ=255) aGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCa < 1:369579/251‑1 (MQ=255) ttCAAAAGACCTTTGCCATTACCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAACGATCCCCCTGCTACCGAATGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGAATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACtc > 2:526780/1‑250 (MQ=255) ttGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAAGTCGCGCAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAACTTTCcggg > 2:131840/1‑250 (MQ=255) gcaattcaagTTTTCGCGCTGTTCCGGCTAACCCAGGAAGCAATCCCCCTGCTACCGAATGGAGCAGATATCTTCACCACGTCGTCACTCCAGGCATACCAGCCAAGTCCGCATTTACTGTACTATCCGGCTACGAAGGCGGCGATTCTGCACTACAGCCGTGGCTTGGCAAAACATGACGCGCAGACAGGAATGCGGGCGAATCTTGTCGCGGCATGCCCGCTCTGCACAGCCCGGCAAATTTCcggcgg > 2:436787/10‑251 (MQ=255) ccATTCACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGCTCCCCCTGCTACCGAAAGGCGCAAGTATCATCCCCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCTTTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAACGGTATTCGGGTGAATATTGTCGAGCCAGGCCCGATCTGGACAGCACTGCAAATTTCcggcgg > 2:241179/1‑250 (MQ=255) ccATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGAATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCcggcggg > 2:559946/1‑250 (MQ=255) aaCGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGAGTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAAc < 1:296058/251‑1 (MQ=255) cGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGACGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGc > 1:499397/1‑251 (MQ=255) tttCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAACGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAgg > 1:481772/1‑251 (MQ=255) gcgcTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCACGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATa > 1:25740/1‑250 (MQ=255) ggCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCg < 1:400415/248‑1 (MQ=255) ggCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGACAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCAGATCTGGACCGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATccagc > 2:136718/1‑246 (MQ=255) cAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCCCTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGAGGCTTGGCAAAACAGGTCGCGAAGAAAAGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGGTTGGTc > 1:563512/1‑250 (MQ=255) aGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTc > 2:58402/1‑249 (MQ=255) aCCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCCGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCAGATGAAACGTg > 2:329274/1‑251 (MQ=255) cGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCg < 1:407280/251‑1 (MQ=255) gAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCgg > 1:316148/1‑251 (MQ=255) aGGTGCAAGTCTCAGCACCACTTCCTCAATCCAGGCATACCAGCCAAGTCCGCATTTAATGGACTATGCGGCTACGAAGGCGGCGATTCTAAACTACAGCCGTGGCTTGGCAAAACAGGCCGCGAAGAAAGGTATTCGGGAGAATATTGTCGCGCCAGGCCCGATCTGGACACCACTGCAAATTTCCGGCGGACACACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGCACCGTGCGGGGc > 2:224873/1‑251 (MQ=255) gCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAAcc < 1:116572/251‑1 (MQ=255) gTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACcggcg > 1:133232/1‑251 (MQ=255) gTATCATCACCACTTCGTCAATCCAGGCATAACAGACAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGCGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCACGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACcggcg > 2:201410/1‑251 (MQ=255) tcaCCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGCCCCGCTCTGGACAGCACTGCAAATTTCCGGCGGACAACCGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTg > 1:268401/1‑251 (MQ=255) accacTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGc < 1:429083/251‑1 (MQ=255) ccGTCAATCCAGGGAGACCAGCCAAGTCCGCATTTACTGGGCTATGCGTCTACGAAGGCGGCGATTCTGAACTACAGCCGGGGCGTGGCAAATCAGGTCGCGAAGAAAGGTAGTCGGGTGAATATTGTCGCGCCAGGCCCGTTCGGGTCAGCGCTGCGAATTTGCGGCGGACAGATGCAGGATGAGAGCCCGCGGGTGGGTCAGCAAACGCGGATGAAACGTGCggggttatcggcatagcggggccctg < 2:268401/249‑23 (MQ=255) caatcagggcatacacgacAAGTACGCCTTTACTGGACTATACGGCTACGAAGGCGGCCATTCTGAACTCCAGCCGTGGCTTGGCAAAACAGGTCGCGCAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCAGCAGATTGGTCAGCAAACGCCGATGAATCGTGCGGGGCAACCGGCGGAACTGGCCCCTGTttat > 2:250381/19‑251 (MQ=255) aacccAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATg < 2:302181/248‑1 (MQ=255) aTCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGt < 1:153040/251‑1 (MQ=255) gCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGCAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGCATATGTTTATCt > 2:204618/1‑251 (MQ=255) gCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCt < 1:718/251‑1 (MQ=255) cATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGTTCAATATTGTCGCGCCAGGCCCGATCt < 2:493226/132‑1 (MQ=255) cATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCt > 1:493226/1‑132 (MQ=255) atgccAGCCAAGTCCGCATTTACTGGCCTCTGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCttg < 2:446570/248‑3 (MQ=255) aGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGCATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGa > 2:197483/1‑195 (MQ=255) aGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGa < 1:197483/195‑1 (MQ=255) gCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTc > 1:227236/1‑251 (MQ=255) cAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAg < 2:481772/251‑1 (MQ=255) cAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCACGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTc > 1:74464/1‑249 (MQ=255) cATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCACACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCg > 1:111996/1‑249 (MQ=255) cTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGt > 1:281441/1‑251 (MQ=255) ggACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATAATTTTTTCTGGCAAGTCAGGCGTCGAGGTACgccc > 2:309115/1‑248 (MQ=255) ggCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACGGGCCCCTGGATATGTTTATCTGGCAAGGCCGGAGGCGAGCTACGGCACCGcgga > 2:489674/1‑246 (MQ=255) gCTACGAAGACGGCGCTTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGt > 1:451192/1‑251 (MQ=255) aaGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGATTCGAGCTACGTCACCGCAGAAGTGCACgg < 1:203174/251‑1 (MQ=255) ggcggcGATTCTGAACTACAGCCGTGGCTTGTCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCg < 2:438805/251‑1 (MQ=255) gcggcgATACTGGCCTACAGCCGTGGCTTGGAAAAACAGGTCGCGCAGAAAGGTATTCGGGTGCATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGGTTGGTCAGCAAACGCCGATGAAACGTGCGGAGCAACCGGCGGAACTGGCCCCTGTATATGTTTAGCCGGCAAGGCAGGATACGAGCTACGCCACCGCCGAAGAGCACGGccg > 2:333823/1‑249 (MQ=255) gcgATTCTGAACTACAGACGTGGCTTGGCAAACCAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCCCGGCGtgtg < 2:479694/251‑1 (MQ=255) aTTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTAGCGCCCGGCCCGATCTGGACAGAACTGCAAATTTCCGCCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTAAATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTgcgg > 1:570155/1‑251 (MQ=255) tACAGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGACGTGCACGGCGTGTGCGGCGGCGAGc > 2:183504/1‑250 (MQ=255) cAGCCGTGGCTTGGCAAACCAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCTGACAAACGCAGTATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCATGAGTCGAGCTACGTCACCACAGAAGTGCACGGCGTGTGCGGCGGCGAGCAtt < 1:171250/251‑1 (MQ=255) aGCCGTGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCAttt < 2:33888/251‑1 (MQ=255) tGGCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGtt > 1:114612/1‑251 (MQ=255) ggCTTGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGtt < 1:526780/250‑1 (MQ=255) ttGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGc > 2:39858/1‑217 (MQ=255) ttGGCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGc < 1:39858/217‑1 (MQ=255) ggCAAAACAGGTCGCGAAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTaaaaa > 1:474491/1‑250 (MQ=255) aaCAGGTCGCGAAGAAAGGTCATCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACCGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTGGGTCAGCAAACGCCGATGAAACGTGCGGGGCACCCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCAGTTAGGTTAAAAAAATGcc < 2:65830/251‑1 (MQ=255) | CCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAGCACTGCAAATTTCCGGCGGACAAACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCC > NC_000913/3150083‑3150555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |