Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,263	G→A	A45T (GCC→ACC)	yghT →	putative ATP‑binding protein YghT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,263	0	G	A	98.3%	190.6 / NA	58	A45T (GCC→ACC)	yghT	putative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (34/23);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CAACCTTATTGTGACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGT  >  NC_000913/3134019‑3134508                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      cAACCTGATTGTGACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGGTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGa                                                                                                                                                                                                                                                  <  1:26984/250‑1 (MQ=255)        aTTGTGACAAGGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTGTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTGATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATgtcg                                                                                                                                                                                                                                           <  2:345069/250‑1 (MQ=255)              aCAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGc                                                                                                                                                                                                                                    <  2:339979/251‑1 (MQ=255)               cAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTGTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACGCCTCCATTAATTGCTGTTATTGGTAGCGATGGTTCAGGTAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAGAATATGGTGCTGGCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCa                                                                                                                                                                                                                                   <  1:56864/251‑1 (MQ=255)                aaTGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTga                                                                                                                                                                                                                                    >  1:178934/1‑248 (MQ=255)                    gTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATGTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGTTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGGTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGAc                                                                                                                                                                                                                              <  1:344481/251‑1 (MQ=255)                       aaTTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACaaa                                                                                                                                                                                                                           <  1:418563/251‑1 (MQ=255)                                aTTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGtt                                                                                                                                                                                                                  >  1:327760/1‑251 (MQ=255)                                   gACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGAt                                                                                                                                                                                                               <  1:27615/251‑1 (MQ=255)                                    aCCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACATCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGAGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGAGCTGCCGAAGGAGTTCATTTAGAAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATg                                                                                                                                                                                                              >  2:418521/1‑251 (MQ=255)                                          aTTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGTATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGAGGGGaaa                                                                                                                                                                                                         >  1:516623/1‑250 (MQ=255)                                              cGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCTGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGGCAAAATTACCGTTGATGGGAAAATccg                                                                                                                                                                                                    <  2:394132/251‑2 (MQ=255)                                                  ttCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTAc                                                                                                                                                                                                 >  1:522769/1‑250 (MQ=255)                                                         aaaCATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGAAATCAATAATGCCTCTTTTAATTGACGTTATTGTTAGCGATGGTTCAGACACGTCAACGGTGTGTGAACATCTTATTAGAGTTGTCGATAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGAACGGAAATGTCGGTCGTGCAGTGACAAAATTACAGTTAAAGGGAAAATCTTTACATAGAAc                                                                                                                                                                                          >  2:66358/1‑250 (MQ=255)                                                         aaaCATAGTTACCAAATAAATATTTTTAAAGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATGAATTGCCGTTAGTGGTAGCGAAGTTTCAAGCAAGTCAACGGTGGGTGAACATCTTATTACCTTTGTCGAAGATTAAGGCTCTCGCGAACGATGTCATTTAGGAATACAGACATGAAATCTCGGTCGTTCAGTAACCAAATTACGGTTACTGGTAAAATGCTTACATaaagca                                                                                                                                                                                         <  2:332826/251‑4 (MQ=255)                                                           aCATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAAt                                                                                                                                                                                       >  1:504261/1‑251 (MQ=255)                                                                       aataaataTTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACCCCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGTAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATGAAACAAGTGAACGAAATCa                                                                                                                                                                           <  2:358140/251‑1 (MQ=255)                                                                          aaataTTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAAGAATTGAACGAAATCAGGt                                                                                                                                                                        <  2:260766/251‑1 (MQ=255)                                                                                      tGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCaa                                                                                                                                                             >  2:439800/1‑250 (MQ=255)                                                                                              taaattaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTg                                                                                                                                                     <  1:21972/250‑1 (MQ=255)                                                                                              taaattaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGc                                                                                                                                                    >  1:29466/1‑251 (MQ=255)                                                                                                 attaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTg                                                                                                                                                 >  2:291659/1‑251 (MQ=255)                                                                                                  ttaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCACGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTgg                                                                                                                                                >  2:243263/1‑251 (MQ=255)                                                                                                           atatTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACAGTTGATGGGAAAATCCTTAAATAAAACAATTGAACAAAATCAGGTGAAAACACCAAAAAAATTGCCTGAACAAGTTcc                                                                                                                                       >  2:320076/1‑251 (MQ=255)                                                                                                            tatTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCACGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCg                                                                                                                                      >  2:56989/1‑251 (MQ=255)                                                                                                            tatTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAAAATATTATTACCGTTGTCGAAAACTATGGTGCTGCCGACAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGCAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCg                                                                                                                                      >  1:293058/1‑251 (MQ=255)                                                                                                               tCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTAAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCAGgcg                                                                                                                                   >  1:376270/1‑251 (MQ=255)                                                                                                                cATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGgcgc                                                                                                                                  >  1:583508/1‑251 (MQ=255)                                                                                                                   gCAGTCAATAACACCTCCATAAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAACTATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATACAACAATTGAACGAAATCAGGAAAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTgg                                                                                                                               >  1:524526/1‑251 (MQ=255)                                                                                                                     aGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCACCGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGAGCTGGt                                                                                                                              >  2:439786/1‑250 (MQ=255)                                                                                                                        cAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATTGGAAAATCCTTACATAAAACAAGTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAAt                                                                                                                           <  2:29466/250‑1 (MQ=255)                                                                                                                            aaCACCTCCATTAATTGCCGTTCTTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGCAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTAc                                                                                                                        >  1:104287/1‑249 (MQ=255)                                                                                                                                   ccATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAAAAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGt                                                                                                               >  1:500119/1‑251 (MQ=255)                                                                                                                                           tGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACATACCGGAAATGTCGGTCGTGCAGTGACAAAATTGCCGTTGATg                                                                                                                                                                                                              <  2:559459/148‑1 (MQ=255)                                                                                                                                           tGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATg                                                                                                                                                                                                              >  1:559459/1‑148 (MQ=255)                                                                                                                                           tGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGAGAAAACAGCCAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTc                                                                                                       >  1:587453/1‑251 (MQ=255)                                                                                                                                           ggCCGTTAGTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTAGTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTc                                                                                                       <  2:358162/250‑1 (MQ=255)                                                                                                                                                           gATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCg                                                                                       >  1:38179/1‑251 (MQ=255)                                                                                                                                                              ggTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTc                                                                                     >  1:262528/1‑250 (MQ=255)                                                                                                                                                                   aGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGc                                                                               >  1:130909/1‑251 (MQ=255)                                                                                                                                                                               ggTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGGCCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTGTCGTCATATGCTTGCCTgtcgtc                                                                   <  2:38179/251‑1 (MQ=255)                                                                                                                                                                                      gAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAAGTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGCCGCTGACTGCGCTTTCGTCATATGCGTGCCCGTCGTCGTCGCgg                                                            <  2:190820/251‑1 (MQ=255)                                                                                                                                                                                          aTCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAACGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTAACGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCCGGTACAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCGGTCGCTTACTGAACTTTCTTAATATGCTTGCCTGTCATCGTCTCGGGtt                                                         >  2:41165/1‑250 (MQ=255)                                                                                                                                                                                          aTCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTc                                                                                                                                        <  1:28327/171‑1 (MQ=255)                                                                                                                                                                                          aTCTTATTACCGATGTCGAAAAATATGGTGCTGCCTAAAGAGTTCATTTAGGCAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAACAAATTGCCTGGACCAGTTc                                                                                                                                        >  2:28327/1‑171 (MQ=255)                                                                                                                                                                                                  aCCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCt                                                <  1:243263/251‑1 (MQ=255)                                                                                                                                                                                                     gTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACCGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAac                                             >  1:553990/1‑251 (MQ=255)                                                                                                                                                                                                       tGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAacc                                            <  2:262528/250‑1 (MQ=255)                                                                                                                                                                                                          cGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAaccgac                                         <  1:56989/250‑1 (MQ=255)                                                                                                                                                                                                               aaTATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCGTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTAt                                   <  1:533678/251‑1 (MQ=255)                                                                                                                                                                                                                      tgctgcCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAgg                            >  1:298448/1‑251 (MQ=255)                                                                                                                                                                                                                         tgcCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGAcc                         >  1:84981/1‑251 (MQ=255)                                                                                                                                                                                                                             gAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAAt                     >  1:104902/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCtt                                                                             >  2:430711/1‑188 (MQ=255)                                                                                                                                                                                                                                    ttCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCtt                                                                             <  1:430711/188‑1 (MQ=255)                                                                                                                                                                                                                                        tttAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGCACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGCCCGTTATCCTCAGGACCAAATTCCTGGCGCtt          >  1:402092/1‑251 (MQ=255)                                                                                                                                                                                                                                           aGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACg       >  1:366685/1‑251 (MQ=255)                                                                                                                                                                                                                                                aaCAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGt  <  1:329113/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CAACCTTATTGTGACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGT  >  NC_000913/3134019‑3134508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑