Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,133,175 G→A T13I (ACC→ATC)  yghR ← putative ATP‑binding protein YghR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,133,1750GA98.4% 194.1 / NA 61T13I (ACC→ATC) yghRputative ATP‑binding protein YghR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/37);  minor base T (1/0);  total (24/37)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTC  >  NC_000913/3132934‑3133420
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       
ggTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCTGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCCGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGAAGTTGGGCTGCGGCGCGATTGTACTAt                                                                                                                                                                                                                                                >  2:588399/1‑251 (MQ=255)
    ttCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAAc                                                                                                                                                                                                                                            >  1:314184/1‑251 (MQ=255)
         gCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACa‑gtt                                                                                                                                                                                                                                       <  1:210484/250‑1 (MQ=255)
           tttGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACa‑gtttg                                                                                                                                                                                                                                     >  1:217266/1‑250 (MQ=255)
              gggTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCAACAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTA‑CACgtttgagtt                                                                                                                                                                                                                                 >  2:331411/1‑251 (MQ=255)
                     cGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGt                                                                                                                                                                                                                           <  2:449301/250‑1 (MQ=255)
                      gAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGc                                                                                                                                                                                                                         >  2:398298/1‑251 (MQ=255)
                         gATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATc                                                                                                                                                                                                                      <  1:319459/251‑1 (MQ=255)
                          aTTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATcc                                                                                                                                                                                                                     <  1:545153/251‑1 (MQ=255)
                              ggCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTc                                                                                                                                                                                                                 <  2:304099/251‑1 (MQ=255)
                                     ggTCGCCGTTCCAGCCAGTCGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAAGGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTCAAat                                                                                                                                                                                                          <  2:461673/250‑1 (MQ=255)
                                     aGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAat                                                                                                                                                                                                          <  1:112561/251‑1 (MQ=255)
                                      gTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAatc                                                                                                                                                                                                         <  1:331080/251‑1 (MQ=255)
                                        cGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAatca                                                                                                                                                                                                        <  1:104093/250‑1 (MQ=255)
                                           cGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGGCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACGATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATccc                                                                                                                                                                                                    <  1:117034/251‑1 (MQ=255)
                                            gTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATcccc                                                                                                                                                                                                   >  1:231967/1‑251 (MQ=255)
                                             ttCCAGCCAGACGCCCACCAACGGCAATCGTTTGCTTTTATCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGCTTTACCGGTGCCATCACACCCGACCACCGCACTCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCt                                                                                                                                                                                                  >  2:252680/1‑251 (MQ=255)
                                              tCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTGTGTAGTGCATCCATGCGTTAAATCATCCCCt                                                                                                                                                                                                  <  1:418334/250‑1 (MQ=255)
                                               ccagccagACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTAt                                                                                                                                                                                                >  1:476752/1‑251 (MQ=255)
                                                    cagACGCCACCCAACGGCTATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCCGCCCCAGATAGCGCCGCTCCGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTCCCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGTTTGTACTTTTAACA‑GTTTGAGTTTGTAGTGCATCCTTTCGTTAAATCATCCCCTATCCAtt                                                                                                                                                                                           >  2:401366/1‑251 (MQ=255)
                                                      gACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTCTACTATTAACC‑GTTTGAGTTTGTAGTGCATCCATTAGTTAAATCATCACCTATCCAtttt                                                                                                                                                                                         >  2:227843/1‑251 (MQ=255)
                                                           caaccaacGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCCGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTgt                                                                                                                                                                                    >  2:234949/1‑250 (MQ=255)
                                                               caacGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATc                                                                                                                                                                                  >  2:141980/1‑249 (MQ=255)
                                                                 acGGCAATCTTTTGATTTTGTCGCCGTCTCCTCCGGAGAGCAGCCACAGATAGCGCCGCTCGGTTTGTCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTTCCATCACACCCGACCACCTCAATCAGCCTCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACT‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTAGCCATTTTGGTGATCAGGa                                                                                                                                                                              <  2:515650/251‑1 (MQ=255)
                                                                    gCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAg                                                                                                                                                                           <  1:107418/251‑1 (MQ=255)
                                                                                 tttGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAAtt                                                                                                                                                              >  1:276623/1‑251 (MQ=255)
                                                                                   tGTCGCCGTCTTCGCCGGGGCGCAGCCCCAGGTAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTg                                                                                                                                                             <  2:431774/250‑1 (MQ=255)
                                                                                   tGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTg                                                                                                                                                             <  1:35946/250‑1 (MQ=255)
                                                                                   tGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTg                                                                                                                                                             <  2:314184/250‑1 (MQ=255)
                                                                                    gTCGCCGTCGTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACGATTAACA‑GTTTGAGTTTGGAGTGCATCCATTCGTGAAAGCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTgcg                                                                                                                                                           <  1:252680/251‑1 (MQ=255)
                                                                                         cGTCTGCGCCGGATAGCAGCCCCAGATAGCGCCGCGCGGTGTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCAGCACTCCCGACCTCCGCAATCAGCCCCGGAATGGAGTTGGGCTGCGGCGCGAGTGTACGAGTAACA‑GTTTGAGTTTGTAGTGCATGCAGGCGTTACATCAGCCCCTATCGATTTTGGGGATCAGTAAAGCGCGGCGTCAATTGCGCGTAg                                                                                                                                                      <  2:306705/251‑1 (MQ=255)
                                                                                              tCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGAc                                                                                                                                                  <  1:106717/250‑1 (MQ=255)
                                                                                               cGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATGCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACg                                                                                                                                                 <  2:263890/250‑1 (MQ=255)
                                                                                                   ggAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGc                                                                                                                                            >  1:159681/1‑251 (MQ=255)
                                                                                                                          gcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATAACACCCGACCACCGCAATCAGCCCCGGTATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGAGTCg                                                                                                                     >  1:114786/1‑251 (MQ=255)
                                                                                                                           cTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCGGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCgggggtgcgc                                                                                                                    >  1:68634/1‑245 (MQ=255)
                                                                                                                           cTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCgggggggcgc                                                                                                                    >  1:35577/1‑245 (MQ=255)
                                                                                                                                   gCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATcc                                                                                                              >  1:252782/1‑249 (MQ=255)
                                                                                                                                    ccAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGTTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGt                                                                                                           <  1:331411/251‑1 (MQ=255)
                                                                                                                                                cAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCttt                                                                                               <  2:223564/251‑1 (MQ=255)
                                                                                                                                                cAGCGATTTCACCAGGTCGGTGGGCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCAGCCAGTTCAAGGATCttt                                                                                               <  2:136028/251‑1 (MQ=255)
                                                                                                                                                       ttCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGCCCactcagtttcaggggatttgtgccat                                                                                        >  2:289017/1‑226 (MQ=255)
                                                                                                                                                          cccAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACTCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGa                                                                                    <  2:138742/250‑1 (MQ=255)
                                                                                                                                                                  ggtggtCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGGGACGCTTGCAGAATTTCATCGGCGTGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGt                                                                            <  1:38648/251‑1 (MQ=255)
                                                                                                                                                                   gtggtCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCATTCAAGGATCTTTTCGcccttttatttcagtt                                                                            >  1:10167/1‑237 (MQ=255)
                                                                                                                                                                     gggcAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTCTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGTCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTg                                                                          <  1:227843/247‑1 (MQ=255)
                                                                                                                                                                            gtgGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTa                                                                   <  1:85874/250‑1 (MQ=255)
                                                                                                                                                                                agttACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGc                                                              <  2:271226/249‑1 (MQ=255)
                                                                                                                                                                                aTTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGc                                                              <  2:179093/251‑1 (MQ=255)
                                                                                                                                                                                       ggTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCAACCCGTTTAAGGGTCTTTTGGCC‑CTTGAATGGCAGTTGCGGCGTAACGGCGGttttt                                                       >  1:234373/1‑251 (MQ=255)
                                                                                                                                                                                            cATCACCCCCCACCCCCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGGACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCTCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTg                                                  <  2:580907/251‑1 (MQ=255)
                                                                                                                                                                                                  acCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCt                                                   <  1:494365/244‑1 (MQ=255)
                                                                                                                                                                                                  acCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCGTTCCAGGATTTTTGCGCCCATT‑TATGGCAGGTTCGGCGTGACGGCGGttttttttttgattgg                                            >  2:494365/1‑241 (MQ=255)
                                                                                                                                                                                                       cccaccGCAATCAGCCCCGGAAGGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTATTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTTAATTGCGCGTAGTGACGCTGGCAGAATGTCATCGGCGGGGTGTCGTCCATCCAGTTCTAGGATCTTTGCGCC‑AGTGAAGGTCAGTTGCGGATTAACGGGGATTTTTTCCTGTAAccatgcct                                       <  2:60643/250‑8 (MQ=255)
                                                                                                                                                                                                         caccGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCCGTTCAAGGAACTTTGCGCC‑CTTTAATGGTAGTTGCGGCGTCAAGGGGATTTTTTCCTCTAAcactccccgt                                     >  2:431462/1‑242 (MQ=255)
                                                                                                                                                                                                              cAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATGTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtggtg                                <  1:71858/251‑1 (MQ=255)
                                                                                                                                                                                                                        cGGAATGTAGTTGGGCTG‑GGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTAc                     <  1:372595/251‑1 (MQ=255)
                                                                                                                                                                                                                                        tGCGGCGCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGCCCATCCAGTTCAAAGATCTTTTTGCC‑ATTGAATGTCAGTTGCGGCGTAACGGGGGTTTTTTTCTGTAACGCTGCCAGTTGGTGGTCCGGTTTACGCGCCAAcgccgtgt      >  2:475478/1‑246 (MQ=255)
                                                                                                                                                                                                                                         gcggcgCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGttt      <  1:289017/250‑1 (MQ=255)
                                                                                                                                                                                                                                           ggcgCGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGtt   <  1:422054/251‑1 (MQ=255)
                                                                                                                                                                                                                                            gcgcGATTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtc  <  2:130111/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       
GGTTTTCATGCTTTGGGTTTTCGAGGATTTGGCCGCCAGTCGCCGTTCCAGCCAGACGCCAACCAACGGCAATCGTTTGATTTTGTCGCCGTCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACA‑GTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCC‑ATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTC  >  NC_000913/3132934‑3133420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑