Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	466,148	G→A	P389S (CCA→TCA)	ybaE ←	uncharacterized protein YbaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	466,148	0	G	A	100.0%	139.9 / NA	43	P389S (CCA→TCA)	ybaE	uncharacterized protein YbaE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/23);  total (20/23)

CAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCT‑AAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCTCCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCAAAT  >  NC_000913/465920‑466387                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTg                                                                                                                                                                                                                            >  1:493902/1‑251 (MQ=255)   aTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGAt                                                                                                                                                                                                                          <  1:452085/251‑1 (MQ=255)     tAATCTGTCGCCCATCATGAGGGCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATACATGACTGGCGGTgttcg                                                                                                                                                                                                                        >  1:143536/1‑247 (MQ=255)      aaTCTGTAGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTACCAGTTTTTTGCGTTATGACAAATAATTGTGAGTGCACAGCCTTCCGCTGCCCGTGTCGCCTGAAGGAGTTCTGACATGGTATGAAGTTCTATCGGTGGGGGCTAAACAAGTGGAAAGGTTTTCGGTAGTTTGCCTTCATCCGGTAACTGCTAATGCGGAATAGACCAGTCTGACAGTAATGCATGACTGGCGGAGAtt                                                                                                                                                                                                                         >  2:46776/1‑248 (MQ=255)           gtggCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGttt                                                                                                                                                                                                                   <  1:16957/247‑1 (MQ=255)           gTCGCCCATCATGAGGTCTGCGTGTGCATGTAGGGTCGTGTCGGCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGAATTCATCCGGTACCTGCCACTGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGCGATCAGGgttt                                                                                                                                                                                                                  >  2:193595/1‑251 (MQ=255)              gCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATAAGGGTTtctc                                                                                                                                                                                                               >  2:113169/1‑251 (MQ=255)                       gAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGGATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGGGGTCAGGGTGTCTCCGACTTCt                                                                                                                                                                                                       >  2:450545/1‑250 (MQ=255)                        aGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTaa                                                                                                                                                                                                     <  1:113169/251‑1 (MQ=255)                             ggCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTGTTTGCGTTATGAAAAATAATGGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCATTCTGCCATGGTATGAAGTACTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTGCATCCGGTACCTGCCAAGGCGGAATAGTCCAGCCGGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGttt                                                                                                                                                                                                <  1:571634/250‑1 (MQ=255)                                  gtgCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGTGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGtaat                                                                                                                                                                                           <  1:501082/251‑1 (MQ=255)                                    gCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGtaataa                                                                                                                                                                                         <  1:17670/251‑1 (MQ=255)                                    gCCTGGAGGGTCGTGTCTTCCCAGTTGTTGGCGTTATAAAAAATAATTGTGAGTTCACAGCCTGCCGCTGCCAGTGACGCCGGTAGGCGTTCTGCCATGGTAAGAAGTTCTAGCGGTAGGTGATAAACCAGCGTAAATGTTTTCGGTAGTGTGACTTCTTCCGGTTCCTGCCAATGCGGAATAGTCCAGTCTGACAGTAATGCATGACTGGCGGTGATCAGGTTGGCTCCGACTTCTAACGGTTGtaataa                                                                                                                                                                                         <  2:556490/251‑1 (MQ=255)                                        gTAGGGACGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATAAGGTTTTTTCCGACTTCTAACGGTTGTAATAAACCg                                                                                                                                                                                     >  2:364016/1‑251 (MQ=255)                                                    cgtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaat                                                                                                                                                                          <  1:480786/250‑1 (MQ=255)                                                        ccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATgg                                                                                                                                                                     <  1:31341/251‑1 (MQ=255)                                                            ttttttGCGTTATAAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGGTCTGCCAGGGTAGGAAGTTCTATCGTTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTTCCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCATGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGgaga                                                                                                                                                                  <  1:41736/250‑1 (MQ=255)                                                                  gCGTTATGAAAAATAATTGTGAGTTCCCAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGGGATAAACCAGCGTCAAGGTTTGCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACttt                                                                                                                                                           <  2:143536/251‑1 (MQ=255)                                                                  gCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGAGCACtt                                                                                                                                                            <  1:129903/250‑1 (MQ=255)                                                                    gTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTc                                                                                                                                                         >  1:92270/1‑251 (MQ=255)                                                                            aaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGGGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGc                                                                                                                                                 <  2:573338/251‑1 (MQ=255)                                                                             aataatTGAGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCCGGTTTTCTCCGTCTTCTAACCTTTGTAATAAAACGGATTGATGGATAATGGAGGACACTTTTCGcgcccgcc                                                                                                                                                >  2:16128/1‑251 (MQ=255)                                                                              ataatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGTGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGATTTCTCCGACTTCTAACGGTTGTCATAACACGGATTGAAGAATAATGGAGGTCCCTTTTCTCGCCTGcc                                                                                                                                                >  2:305155/1‑250 (MQ=255)                                                                              ataatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAACTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGTTCAGGTTTTCTACGGCTTCTAAAGTTTGTAATAAACCGGCTTGATGAATAATGTAGATCACTTTTCTCGCCTggcc                                                                                                                                               >  2:19456/1‑248 (MQ=255)                                                                                 atTGTGAGTTCACAGACTTCCGCTTCCAGTGTCGCCTGTAGGCGTTCTGACATGGTATGAAGTTCTATCGGTCGGCGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTAATCCGGTCCCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTTCGACTTCTAACGGTTTTAATAAACCGGGTTGGGGAATAATTGAGCTTCCTTTTCGCGCCTGcccggg                                                                                                                                            >  2:301670/1‑247 (MQ=255)                                                                                                    ccGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACtttt                                                                                                                          <  1:69867/250‑1 (MQ=255)                                                                                                     cGCTGCCATTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTgc                                                                                                                        <  2:493902/251‑1 (MQ=255)                                                                                                             gtgtCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGATTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTTCGCAACggc                                                                                                                >  1:556717/1‑249 (MQ=255)                                                                                                                        aGGCGTTCGGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCaaa                                                                                                      <  2:132552/250‑1 (MQ=255)                                                                                                                                      ggTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCT‑AAACTGATGc                                                                                      <  1:450545/251‑1 (MQ=255)                                                                                                                                       gTATGCAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGGTTGATGaa                                                                                                                                                                           >  2:238241/1‑166 (MQ=255)                                                                                                                                       gTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaa                                                                                                                                                                           <  1:238241/166‑1 (MQ=255)                                                                                                                                        tATGAAGATCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGAATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAAAGGTTTTAATAAACCGGATTGATGAATAATGGAGATCCCTTTTCGCGCCTGCCAGAGGGAGGGTCGGGGGCTTTTGCGCAACGTCCAATAGCAAAAACCTCAAACtaggccc                                                                                     >  2:197082/1‑245 (MQ=255)                                                                                                                                          tGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCCGAGGGGGAGGCGGGGGCTTTTGCGCAAAGTTCAATAGCAAAAACCT‑AAACTGATGCAGCt                                                                                  >  1:374482/1‑251 (MQ=255)                                                                                                                                          tGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCT‑AAACTGATGCCGc                                                                                   <  2:194172/250‑1 (MQ=255)                                                                                                                                           gAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCT‑AAACTGATGCCGCTa                                                                                 <  1:5738/251‑1 (MQ=255)                                                                                                                                                                    cAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATACCTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGGCTTTTGGGCAACGGCAAATAGCAAAAACCT‑AAACTGATTCCGACACTGACCTGGCTGACCCGTTGGAGcc                                                        >  2:206805/1‑250 (MQ=255)                                                                                                                                                                    cAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAAGAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCT‑AAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGct                                                        <  1:364016/251‑1 (MQ=255)                                                                                                                                                                              gTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAAAGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGGCTTTTGCGCCAAGGCCAATAGCCAAAACCT‑AAAACGATGCCGCTACTGACCTGGGTGTCCCGTTGCAGCACCCCCgggtt                                              >  1:112465/1‑248 (MQ=255)                                                                                                                                                                                   cGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAGATAGCAAAAACCT‑AAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCTCCGGTTTGCCg                                          <  1:194873/250‑1 (MQ=255)                                                                                                                                                                                                                 aTGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGGCTTTTGCGCAACGTCAAATAGCAAAAAACT‑AAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCCGCTCCTCCGGTTTGCCGATGGTGGTTTTCCCGGGGTGCCGACAACTgg           >  1:28431/1‑251 (MQ=255)                                                                                                                                                                                                                     ggAATAGTCCAGCCTAACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTTCGCAACGTCAAATAGCAAAAACCT‑AAACTGATGCCGCTACTGACCTGGCTGACCCGTTTCCGCTCCTCCGGGTTGCCGATGGGGATTTGCACGGGATGCCGACAACtcggtcc       >  2:420153/1‑245 (MQ=255)                                                                                                                                                                                                                          aGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCT‑AAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCTCCGGTTTGCCGAGGGTGATTTGCACGGGATGCCGACAACTGTTTCCCagat  <  2:390102/251‑4 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCT‑AAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCTCCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCAAAT  >  NC_000913/465920‑466387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑