Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,260	T→C	V326A (GTG→GCG)	galP →	galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,260	0	T	C	98.9%	282.4 / NA	90	V326A (GTG→GCG)	galP	galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base C (46/42);  minor base A (1/0);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

AGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCAT  >  NC_000913/3089014‑3089506                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       agaACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTAACGAACAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCCCCTTTATCGCAATCGGACTTGTTGACCGCTGGGGACGTAAACCAACGCTTACGCTGGGCTTCCTGGCGATg                                                                                                                                                                                                                                                    >  2:318944/1‑251 (MQ=255)     cAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGgctg                                                                                                                                                                                                                                                <  1:122765/251‑1 (MQ=255)     cAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGgctg                                                                                                                                                                                                                                                >  1:44160/1‑251 (MQ=255)     cAGCAACGTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTTCCCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCGGGGCTTCCTTGCGATGgctg                                                                                                                                                                                                                                                <  2:213209/251‑1 (MQ=255)      aGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCCACGCTCACGCTGGGCTTCCTGGCGATCgctcg                                                                                                                                                                                                                                               >  2:288269/1‑249 (MQ=255)      aGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGAt                                                                                                                                                                                                                                                     <  1:288269/245‑1 (MQ=255)       gCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCCGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAACTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGCCCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGCCGTAAACCAACGCTAACGCTGGGCTTCCTGGCGTTGgctgct                                                                                                                                                                                                                                              >  1:279028/1‑251 (MQ=255)                gccgcGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATGCACCGGGATGAACGTCAGCATGTATTACGCGCCGAAAATCTTCGAACTGGCGTGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACGTGCCACCTTTATCGCAATCGGCCGTGTTGACCGCTGTGGACGTAAACCATCGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGc                                                                                                                                                                                                                                     <  2:588926/251‑1 (MQ=255)                       ggTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCg                                                                                                                                                                                                                              <  1:226103/251‑1 (MQ=255)                          gtTCCTTGGCGTACTGTTGCAGGTAATGCAGCACTTCCCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACAATACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTACCGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGt                                                                                                                                                                                                                            >  2:572017/1‑250 (MQ=255)                              cTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTATTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGGACTCGGTACAat                                                                                                                                                                                                                       >  1:362667/1‑251 (MQ=255)                                         gTTGCAGGTAATGCAGCAATTCCCCGGGATGAAAGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCGGGCGATGGCTGCTGGCAGGGGCGTACTCGGGACAATGATGCATATCg                                                                                                                                                                                                            <  2:285962/251‑1 (MQ=255)                                            gCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTGCGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAAGGTGGGGGACCGTGATTGGCGGCCTGACCAACGGACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAGCGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATTCATATCGGTa                                                                                                                                                                                                         <  2:235277/251‑1 (MQ=255)                                                gTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACACTGATGCATATCGGTATTcc                                                                                                                                                                                                     >  2:131270/1‑250 (MQ=255)                                                     gcagcaATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGATCTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACtctc                                                                                                                                                                                                <  1:54606/251‑1 (MQ=255)                                                          aaTTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCg                                                                                                                                                                                           <  1:475303/251‑1 (MQ=255)                                                           aTTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCgg                                                                                                                                                                                          <  2:211110/251‑1 (MQ=255)                                                            ttCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTATCCGTCGgc                                                                                                                                                                                         >  1:416953/1‑251 (MQ=255)                                                               cccGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAAAACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTTACTCTCCGTCGgcgc                                                                                                                                                                                       >  2:5412/2‑250 (MQ=255)                                                                 cggtaTGAACGTCATCATTTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATCCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGt                                                                                                                                                                                    <  2:449405/247‑1 (MQ=255)                                                                  gggATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTa                                                                                                                                                                                   <  1:582964/251‑1 (MQ=255)                                                                   ggATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGGATTCACTCTCCGTCGGCGCAGTAt                                                                                                                                                                                  >  1:504451/1‑251 (MQ=255)                                                                      tGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCat                                                                                                                                                                                                                >  1:177662/1‑218 (MQ=255)                                                                      tGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCat                                                                                                                                                                                                                <  2:177662/218‑1 (MQ=255)                                                                             catcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGCTGGCTGCTGGCATGGGCGTACTCGGTACAACGAGTCATCTCGGTATTCACTCTCCGTCGGCGCAGGATTtcgccatcg                                                                                                                                                                        >  2:51874/1‑251 (MQ=255)                                                                              atcatGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtcgccatcgc                                                                                                                                                                       <  1:546525/251‑1 (MQ=255)                                                                                        aCGCTCCGAAAATCTTCGAACTGGCGTGTTATTCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGTCCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACTCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGctgctg                                                                                                                                                             <  1:351244/251‑1 (MQ=255)                                                                                        aCGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGctgctg                                                                                                                                                             <  1:54666/251‑1 (MQ=255)                                                                                          gcgcCGAAACTCTTAGAACTGGAGGACTATACCAAGACTACAGCGCCAATGTGGGGGACCGAGGTTGTCGGCCCGACCACCGCCCCTGCCCCCTTTATCGCCATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGGACTCGGTACAACGATGCATATCGGTATTCACTCTCCGTCGGCGCCGTATTTCGCCATCGCCATGCTGCTGAt                                                                                                                                                           >  2:253849/1‑251 (MQ=255)                                                                                             ccGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGtt                                                                                                                                                        <  2:44160/251‑1 (MQ=255)                                                                                               gAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGAACTTGCCACCTTTATCGCAATCAGCCTTGTTGGCCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATTCATATCGGTCTTCACTCTCCGTCGGCCCCGTATTTCGCCATCCCCATGCTGCTGATttttt                                                                                                                                                      >  2:516147/1‑250 (MQ=255)                                                                                                 aaaTCTTCTAACTGGCGGGTAATACCAACACTTCCGATAAAATGTGGGAGACCGTGATTGTCTGCCTGACCAACGAACTTGCCTCCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACATAAACCAACGTTAACGCTGGGCTTCCTGGCGATGGCTGCTTGCATGGGCGTACTCTGTACAATGATGCATATCTGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCGGATGtttctt                                                                                                                                                    <  2:444731/251‑4 (MQ=255)                                                                                                       tCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCgg                                                                                                                                               <  1:239934/250‑1 (MQ=255)                                                                                                              ggCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGcc                                                                                                                                        >  1:30617/1‑250 (MQ=255)                                                                                                              ggCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTAATTGCCACCATTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTGCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGAAGCATATCGGGATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCa                                                                                                                                       <  1:252324/251‑1 (MQ=255)                                                                                                                caggTTATACCAACCCTACCGAGCAAATGTGGGGGACCGTGATTGTCTGCCTGACCAACGCACTTGCCCCCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATAGCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGtttttccctg                                                                                                                                     >  2:370025/3‑245 (MQ=255)                                                                                                                 gggTTATACCAACACTACCGAGCAAATGTGGTGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATg                                                                                                                                     >  2:51818/1‑250 (MQ=255)                                                                                                                       taCCACCACTACCGCGCCCATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTCCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTACACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCCTGGGCGTACTCGGTACAATGATGCCTATCGGTCTTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTTTTGTCGGTTTTGCCATGAggtccg                                                                                                                              >  2:88801/1‑246 (MQ=255)                                                                                                                       taCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCg                                                                                                                              >  2:453304/1‑251 (MQ=255)                                                                                                                           aaCACTACCGAGCAAATGTGGGGGACCGTGATTGTCGTCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTcc                                                                                                                          <  1:253849/251‑1 (MQ=255)                                                                                                                           aaCACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTcc                                                                                                                          <  1:93190/251‑1 (MQ=255)                                                                                                                            acacTTCCGAGCAAATGTGTGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGTCTTCCTGGCGATGGCTTCTGGCATGGGCGTACTCGGTACAAGGATGCATATCGTTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCg                                                                                                                         <  1:356448/251‑1 (MQ=255)                                                                                                                              ccTACCGAGCAAATGTGGGGGACCTTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTACTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCTGTCCGCt                                                                                                                       >  2:483643/2‑251 (MQ=255)                                                                                                                                 cccGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTTCTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGag                                                                                                                    <  2:25200/250‑2 (MQ=255)                                                                                                                                    gAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCCGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTg                                                                                                                 >  2:505687/1‑251 (MQ=255)                                                                                                                                    gAGCAAATATCGGGGACCGTGATTATCGGCCTGACCAACGTACTTGCCAACTTTATAGCAATCGGCCTTGTTGAACGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGCCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCAAGCGGCTGATTTTTATTGTCGGATTTGCCAAGATTGGCGGTCCGCTGAtttt                                                                                                                 >  2:563795/1‑250 (MQ=255)                                                                                                                                        aaaTGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGt                                                                                                              >  1:119433/1‑250 (MQ=255)                                                                                                                                        aaaTGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAAGGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGGCGGGTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTa                                                                                                             <  2:399409/251‑1 (MQ=255)                                                                                                                                          aTGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACt                                                                                                           >  1:287048/1‑251 (MQ=255)                                                                                                                                            gtgGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACtg                                                                                                          <  1:45551/250‑1 (MQ=255)                                                                                                                                              gggggACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGc                                                                                                       >  2:73275/1‑251 (MQ=255)                                                                                                                                               ggggACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCt                                                                                                      <  1:73275/251‑1 (MQ=255)                                                                                                                                                        gATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTc                                                                                             >  1:257599/1‑251 (MQ=255)                                                                                                                                                         aTTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTc                                                                                             >  1:208593/1‑250 (MQ=255)                                                                                                                                                            gtccgCCTGACCCACCTCCTTGCCCCCTTTATCGCACCCCGCCTTGTTGTCCGCTGGGGACGTACACCACCGCTAACGCTGCGCTTCCTGGCGCCCGCTGTTGTCATGGGCGTATTCTTTACACTGATGCACAGCTTTATTCCCTCCCCGTCGTCGCAGTATTTCGCCATCGCCCTGCTGCGCATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTAGGTCCTGTGCTCCGAAATTCAGcc                                                                                         <  2:195564/247‑1 (MQ=255)                                                                                                                                                              cGGCCTGACCAACGTACTTGCCACCTTTCTCGCAATCGGCCTTGTTGACCGCTGGGGACGTACACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCACTGAGTGCCGGTCCGCTGATTTGGGTACTGAGCTCCGAAATTCAGCCGc                                                                                       >  2:560613/1‑251 (MQ=255)                                                                                                                                                                gCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTg                                                                                     >  1:243278/1‑251 (MQ=255)                                                                                                                                                                 ccTGACCAACGTACTTGCCACCTTTATCGCGATCGGCATTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCgatg                                                                                     >  2:494839/1‑247 (MQ=255)                                                                                                                                                                    gACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGGCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGaaa                                                                                  <  1:572017/250‑1 (MQ=255)                                                                                                                                                                       cAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTTGGCTTCCTGGCGATGGCTGCGGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGAAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTAATGTGCTCCGAAATTCAGCCGCTgcacgtcc                                                                              >  2:495950/1‑244 (MQ=255)                                                                                                                                                                       cAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGcc                                                                              >  1:362119/1‑251 (MQ=255)                                                                                                                                                                          cGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCTGTATTCACTCTCCGTCGGCGCAGTATTGCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGTTCCGGTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCcgcg                                                                           <  1:232898/251‑1 (MQ=255)                                                                                                                                                                                     ccTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGACATTCAGCCGCTGAAAGGCCGCGATTTTGGCATc                                                                >  1:7802/1‑251 (MQ=255)                                                                                                                                                                                     ccTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATc                                                                >  1:520339/1‑251 (MQ=255)                                                                                                                                                                                        ttATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCa                                                               <  2:120154/249‑1 (MQ=255)                                                                                                                                                                                              cAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTcc                                                       <  1:132603/251‑1 (MQ=255)                                                                                                                                                                                                  cGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACt                                                    <  2:133668/250‑1 (MQ=255)                                                                                                                                                                                                  cGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGATCCACTg                                                   >  2:463573/1‑251 (MQ=255)                                                                                                                                                                                                   ggctttgttgACCGCTGGGGGCGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCGCCGTCGGCGCAGTATTTCGCCATCTCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTTCCGGTCCGCGGATTTGGGTGCTGTGCTCCGAAATTCAGCCGCTGAAATGCCGCGATTTTGGCATCACCTGCTCCACTGc                                                  <  2:90014/247‑1 (MQ=255)                                                                                                                                                                                                   ggCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGc                                                  >  1:161502/1‑251 (MQ=255)                                                                                                                                                                                                       ttgttgACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGccacc                                              >  1:464895/1‑251 (MQ=255)                                                                                                                                                                                                        tgttgACCGCTGGGGACGTAACCCAACGCTCACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGTGTGTACGCGGTACAATGATGCATATCGGTATTCACTCTCCGTCTGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCTGTCCGCTGATTTGGGTACTGTGCTCCTAAATTCCGCCGCTGAAAGGCCGCTATTTTTGCATCACCTGCTCCATTGccacc                                              <  2:383234/250‑1 (MQ=255)                                                                                                                                                                                                        tgttgACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGccacc                                              <  1:51874/250‑1 (MQ=255)                                                                                                                                                                                                        tgttgACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGccac                                               <  1:453304/249‑1 (MQ=255)                                                                                                                                                                                                        cgttgTCCGCTGGGGACGGAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCAAGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATCTTTATTGTCGGTTTTGCCATGAGTTCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATGCAGCCGCTGAAAGGCTGCGATTTTGGCATCACCTGCTCCACTGccac                                               <  1:517707/248‑1 (MQ=255)                                                                                                                                                                                                          ttgACCGCAGGGGCCGTAAACCAACGATAAAGCTGGGCTTCCTGGAGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATAGGTATTCACTATCCGTCGGCGCAGTATTTCGCAATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTAAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAAc                                           >  2:273761/1‑251 (MQ=255)                                                                                                                                                                                                             aCCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTgg                                        >  1:312406/1‑251 (MQ=255)                                                                                                                                                                                                                       aCGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCCCCAACTGGATTGCCAAc                               >  2:351419/1‑250 (MQ=255)                                                                                                                                                                                                                          tAAACCACCGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTAAAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGCTTGCCAACATgc                           >  2:226821/1‑250 (MQ=255)                                                                                                                                                                                                                            aaCCAACGCTACCGCTGGGCTTCATGGCGATGTCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCCCTGCCACCAACTGCATTGCCAACATgctc                         >  2:94482/1‑248 (MQ=255)                                                                                                                                                                                                                                aacgctaacgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTg                     >  1:352373/1‑251 (MQ=255)                                                                                                                                                                                                                                 acgctaacgctGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCGCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTgg                    <  1:226821/251‑1 (MQ=255)                                                                                                                                                                                                                                        cgcgggtctgccTGGCGATGGCTGCTGGCAGGGGGGTACCCGGTTCAATGATGTATATCGGTATTCTCTCTACGGCGGCGCAGTACGTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTGTTGGCATGAGTGCCGGTCCGCTGATGTGGGTGCTGTGCTCCGTAATTCAGTCGCTGAACGGCCGCGAGTTTGGCATCACCTGCTCCACTGCCACCAACTTGAGTGCCAACATGATTGTTGGCGCAACg             <  2:406844/241‑1 (MQ=255)                                                                                                                                                                                                                                            gggCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTGGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTc          <  1:560613/250‑1 (MQ=255)                                                                                                                                                                                                                                             gggttCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTAGAATTATGCAGAGTGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCAGCGCCATGCTGCTGATGTTTATTGTCGGTGTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCGGAAAGGCCGCGATTTTGGCATCACCTGCTGCGCTTCCACCAGCTGGATTGCCAAGATGATCGTGGGGGCAGCGTTCCt        <  2:362667/248‑1 (MQ=255)                                                                                                                                                                                                                                               cTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTg       >  1:266594/1‑250 (MQ=255)                                                                                                                                                                                                                                                tcccTGGCGATGGCTGCTGGCATGGGCTTACTCGGTACAATGATGCATATCGGTATTCACTCGCCGTCGGCGCAGTATGTCGCCATCGCCATGCGGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCTCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGGTGGCGCAACGTTCCTGa      <  2:134576/248‑1 (MQ=255)                                                                                                                                                                                                                                                  ccTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGAcc    >  1:2152/1‑250 (MQ=255)                                                                                                                                                                                                                                                   cTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAAAATGATCGTTGGCGCCACGTTCCTGACCac  >  2:8672/1‑250 (MQ=255)                                                                                                                                                                                                                                                   cTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGACGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCAt  >  1:555806/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCAT  >  NC_000913/3089014‑3089506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑