Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,065,449 G→A L216L (TTG→TTA)  scpC → propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,065,4490GA94.9% 229.6 / NA 82L216L (TTG→TTA) scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (32/43);  minor base C (3/1);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGG  >  NC_000913/3065205‑3065690
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 
aTGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGAc                                                                                                                                                                                                                                             <  1:538468/251‑1 (MQ=255)
   gTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGcc                                                                                                                                                                                                                                          <  1:554056/251‑1 (MQ=255)
   gTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATAGTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGAGTGGCGCCGTCTTGGAATCCAACTTACCCGACGcc                                                                                                                                                                                                                                          <  2:388132/251‑1 (MQ=255)
            ggTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGTGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATTTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATg                                                                                                                                                                                                                                 <  1:280888/251‑1 (MQ=255)
            ggTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATg                                                                                                                                                                                                                                 <  1:296109/251‑1 (MQ=255)
                      cGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGc                                                                                                                                                                                                                       <  1:426411/251‑1 (MQ=255)
                             ggTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATcc                                                                                                                                                                                                                >  2:422888/1‑251 (MQ=255)
                             ggTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATcc                                                                                                                                                                                                                <  1:211908/251‑1 (MQ=255)
                                aaTGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCa                                                                                                                                                                                                              <  1:494045/250‑1 (MQ=255)
                                   gcgcCGACCTGGCTCCTGCGGGCGAAGAAAGTGATCAGTGAGCTCAATCACTATCACGATCCTCGCGTTGCAGAACTGGCGGATAGTGTGATTCCTGGTGCTCCTCCGCGGCGCAATAGCGTGTCGATCGTCCATGCATTTGATCGCGTCTGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAATTTTCCCTACGCCGGTAATATGCTGGATAATCAAAGTCCCAtgtg                                                                                                                                                                                                          <  2:275488/251‑1 (MQ=255)
                                      ccGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCca                                                                                                                                                                                                       <  1:427745/251‑1 (MQ=255)
                                        gACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcagc                                                                                                                                                                                                     <  1:253638/251‑1 (MQ=255)
                                        gACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcagc                                                                                                                                                                                                     >  1:6554/1‑251 (MQ=255)
                                        gACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcagc                                                                                                                                                                                                     >  1:173946/1‑251 (MQ=255)
                                            tGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATTTGCcagc                                                                                                                                                                                                     >  1:429155/1‑247 (MQ=255)
                                             ggCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGAtt                                                                                                                                                                                                <  1:427800/251‑1 (MQ=255)
                                              gctgctGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTg                                                                                                                                                                                               <  1:242457/251‑1 (MQ=255)
                                               ctgctgCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGc                                                                                                                                                                                              >  1:199255/1‑251 (MQ=255)
                                                   ggcGGGCGAAGAAAGGGATCAGTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGAAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGAt                                                                                                                                                                                          <  1:152962/250‑1 (MQ=255)
                                                          gaagaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTgg                                                                                                                                                                                   >  1:580559/1‑251 (MQ=255)
                                                          gaagaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGGCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGCTTGCCGATAACgggg                                                                                                                                                                                   >  2:438203/1‑248 (MQ=255)
                                                            agaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGCCGCCGGTAATATGCTGGTTAAGCAAAATCCCATGTGCCCGCAGATTGCCGATAACGTGGTc                                                                                                                                                                                 >  2:361082/1‑251 (MQ=255)
                                                            agaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTc                                                                                                                                                                                 <  1:189605/251‑1 (MQ=255)
                                                             gaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAATTACCCGACGACGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTc                                                                                                                                                                                 >  1:384157/1‑250 (MQ=255)
                                                              aaaGTGATCATTGAACTCAATCACTATCACGCTCCGCGCGTTGCCGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAAACAACTTCCCCGACGCCGGTAATATACTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACgatgacac                                                                                                                                                                               >  1:508372/1‑244 (MQ=255)
                                                               aaGTGATCATTGAACTCAATCACTATCACTATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCGTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATGGTCGCCGTCGTGGAAACCAACTTTCCCGATGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCGGCAGATTGTTGGTAACGTGGTCACg                                                                                                                                                                              <  2:40560/251‑1 (MQ=255)
                                                                      cATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAt                                                                                                                                                                       >  1:400635/1‑251 (MQ=255)
                                                                      cATTGAACTCAAGCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTa                                                                                                                                                                        <  2:43011/250‑1 (MQ=255)
                                                                       aTTGAACTCAATCACTATCACGAGCCGCGCGATGCAGAACTGGCGGCTATGGTTATTCCTGGCGCGCCACCACGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGACGTGGAAACAAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATCTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAtt                                                                                                                                                                      >  1:414694/1‑251 (MQ=255)
                                                                         tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGCTCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGc                                                                                                                                                                    >  2:570694/1‑251 (MQ=255)
                                                                         ggAACTCAATCACGAGCACGATCCGCGCGTTGCAGAACTGGCGGATAGTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCGGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCAGGTTCTTATTGc                                                                                                                                                                    <  2:62396/250‑1 (MQ=255)
                                                                          gAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGc                                                                                                                                                                    <  1:516471/250‑1 (MQ=255)
                                                                             cTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATCTTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCCGATTGCCGATAACGTGGTCACGGTCTTATTGCAgg                                                                                                                                                                 >  2:548584/1‑250 (MQ=255)
                                                                               caagcACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGAGTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGAGTGTCGCCGTCGTGGAAACCAGCGTGCCCGTCGCCGGTAATAGGCTGGATAATCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGaaa                                                                                                                                                              <  1:333261/247‑1 (MQ=255)
                                                                               cAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGaa                                                                                                                                                               >  1:425116/1‑250 (MQ=255)
                                                                               cAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGa                                                                                                                                                                <  2:118562/249‑1 (MQ=255)
                                                                               cAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGa                                                                                                                                                                <  2:173946/249‑1 (MQ=255)
                                                                                   cACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGgc                                                                                                                                                          <  1:375552/251‑1 (MQ=255)
                                                                                       atcgcGATCCGCGCGTGGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTTCCCGCTATGTGCAAAGCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATAGGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAGCGTGGTCACGTTCTTATTGCAGGAAATGgcgc                                                                                                                                                        <  1:515183/245‑1 (MQ=255)
                                                                                       aTCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGgcgc                                                                                                                                                        <  1:427928/249‑1 (MQ=255)
                                                                                         cACGATCCGCGCGTTGCGGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATgg                                                                                                                                                    >  1:547407/1‑251 (MQ=255)
                                                                                         cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATgg                                                                                                                                                    <  1:9825/251‑1 (MQ=255)
                                                                                           cGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGc                                                                                                                                                  >  1:195395/1‑251 (MQ=255)
                                                                                                    cgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTccgc                                                                                                                                         >  2:587037/1‑251 (MQ=255)
                                                                                                          aGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAAt                                                                                                                                   <  1:260808/251‑1 (MQ=255)
                                                                                                                 gCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTACTATGCTGGCTAAACAAAATCCCACGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAACTGGCGCATGGGCGTATTCCGCCGGCATTTCTGcc                                                                                                                            >  1:81171/1‑251 (MQ=255)
                                                                                                                    gATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCCGAtt                                                                                                                                                                                                >  1:291447/1‑180 (MQ=255)
                                                                                                                    gATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGAtt                                                                                                                                                                                                <  2:291447/180‑1 (MQ=255)
                                                                                                                             aTTCCTGACGAGCAACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATAGCGTCGCTACCCGCTATGTGCAAATCGATCAGAAACAGATTGACGCCGTCGTGGAAACCAACTTACCCGACGCCGGTACTATGCTGGATAAGCAAAATCCCATGCGCCAGCAGATTGCAGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGACGCTGCaacgagg                                                                                                                >  2:388018/1‑246 (MQ=255)
                                                                                                                                ccTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGt                                                                                                             <  1:570694/251‑1 (MQ=255)
                                                                                                                                  tGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGAAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTgg                                                                                                           >  1:433496/1‑251 (MQ=255)
                                                                                                                                     cgcgcCACAGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCAATGCGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGCAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATAGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGc                                                                                                         >  2:496027/1‑250 (MQ=255)
                                                                                                                                         ccACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAatat                                                                                                    <  1:451816/251‑1 (MQ=255)
                                                                                                                                          cACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAACGTGGCGTGGGCAATATc                                                                                                   >  2:172361/1‑251 (MQ=255)
                                                                                                                                          cACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATc                                                                                                   <  2:6554/251‑1 (MQ=255)
                                                                                                                                          cACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATc                                                                                                   >  1:324132/1‑251 (MQ=255)
                                                                                                                                             cgcgGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGACGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCaag                                                                                                <  2:121470/251‑2 (MQ=255)
                                                                                                                                                ggcgCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCaataat                                                                                             <  1:177635/251‑1 (MQ=255)
                                                                                                                                                  cgcAATAGCGTGTCGATCTTCCATGCAATGGAGCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGGGTGCAATATCAATAATGc                                                                                           <  2:365721/251‑1 (MQ=255)
                                                                                                                                                    cAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCTGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCgg                                                                                         <  1:536045/251‑1 (MQ=255)
                                                                                                                                                     aaTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGt                                                                                        <  2:81171/251‑1 (MQ=255)
                                                                                                                                                        aGCGTGTCTATTTGCCATGCAATGGATCGGGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTCCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCATGAAACGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCGAAGTGGCGGGGGCAAGATCAATAATGCGGTAAt                                                                                     <  2:405383/251‑1 (MQ=255)
                                                                                                                                                          cGTGTCGATCTTCCATGCAATGGATCGAGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCACAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAACGTGGCGTGGGCAATATCAATAATGCGGTAATgg                                                                                   >  1:278732/1‑251 (MQ=255)
                                                                                                                                                             gtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGgcg                                                                                 <  2:199255/250‑1 (MQ=255)
                                                                                                                                                             gtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGgcg                                                                                 <  2:196511/250‑1 (MQ=255)
                                                                                                                                                             gtCGATCTTCCATGCAATGGAGCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGgcg                                                                                 <  2:347352/250‑1 (MQ=255)
                                                                                                                                                                    ttCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCACATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAAAGCGGGAATGGcgcgccttgg                                                                         >  1:395258/1‑245 (MQ=255)
                                                                                                                                                                                            aCCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTACTTCCTCCGtt                                                 <  1:432688/251‑1 (MQ=255)
                                                                                                                                                                                            aCCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGtt                                                 >  1:216980/1‑251 (MQ=255)
                                                                                                                                                                                                gCTATTTGAAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAATTACCCGAGGCCGGTAATATGCTGGACAAGCAAAATCCCCTGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGAAGGAAAGGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGTCGCGTCTGGGGGAAAAACCGGTAATTCCTCCGTTTatt                                             >  2:278155/1‑250 (MQ=255)
                                                                                                                                                                                                      tgCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGCAACCACCTTACCCGAGGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCACAGTGGCGTGGGCACTATCAATAATGAGGAGATGGCCCGTCTGGGGGAAAACCCGGCAATTCCTCCGTTTatgatggat                                       >  2:323434/1‑248 (MQ=255)
                                                                                                                                                                                                      ggCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGGTAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATGTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTTTGATGTa                                        <  2:384941/249‑1 (MQ=255)
                                                                                                                                                                                                          aaTCGATCCGAAACAGCTTGTCGCCGTCGTGGAAAAAAACTTCCCCGACGCCGGCAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGACAACGTGGTCACGCTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTAGCGTGGGCAATATCAATAATGCGGGAATGACGCGTCTGGGGGGAAAACCGGGAATTATTCCGTTTATGATGTATTCgg                                   >  2:19434/1‑251 (MQ=255)
                                                                                                                                                                                                                    aaaaaGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACa                         <  1:405279/251‑1 (MQ=255)
                                                                                                                                                                                                                       aaGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTGATTCCTCCGTTTATGATGTATTCGGAAGGGCTACAgg                       >  2:11827/1‑250 (MQ=255)
                                                                                                                                                                                                                        aGATTGTGGCAGTCGTGGAAACCAACTTCCCAGACGCCGGTAATATGCAGGATAAGAAAAATCCCATGTGCCAGCAGATTGCCGATAACGTAGTAACGTTCTTCTTGCAGGAAATGACGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCACTATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAAACCGGTCATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGa                      >  2:226488/1‑250 (MQ=255)
                                                                                                                                                                                                                          aTTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATc                   <  2:195395/251‑1 (MQ=255)
                                                                                                                                                                                                                             gTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCgg                 <  2:183778/250‑1 (MQ=255)
                                                                                                                                                                                                                               cGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggtgg              <  1:587037/251‑1 (MQ=255)
                                                                                                                                                                                                                                      gtGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGGGGTGCAttt       >  1:156917/1‑251 (MQ=255)
                                                                                                                                                                                                                                       tGGAAACCACCTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGAAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACCGGAATCGGTGGTGCAttt       >  1:468775/1‑250 (MQ=255)
                                                                                                                                                                                                                                           aaCCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAAGCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCGCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCTGGGGTGCATTTACTgg  <  2:272427/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 
ATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGG  >  NC_000913/3065205‑3065690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑