Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,050,472	G→A	L66L (CTG→TTG)	gcvT ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,050,472	0	G	A	97.1%	231.8 / NA	70	L66L (CTG→TTG)	gcvT	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/40);  minor base C (2/0);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATC‑TTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTG  >  NC_000913/3050227‑3050711                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 cACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGCAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACt                                                                                                                                                                                                                                             >  1:540026/1‑251 (MQ=255)              gAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCt                                                                                                                                                                                                                                <  2:239956/251‑1 (MQ=255)              gAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCt                                                                                                                                                                                                                                >  2:529259/1‑251 (MQ=255)                 aGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGcc                                                                                                                                                                                                                             >  2:249466/1‑251 (MQ=255)                     catcaCGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCcgcg                                                                                                                                                                                                                          >  1:237857/1‑250 (MQ=255)                          cGAACGGTAATTTCGATGCTGAACGGTTCAGCGTGTTGTGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGGTAACAACGAGGCGGAAGAAATCTTCAGTAAATTATAAGCCGATGAGGTCCTCTATCACCCCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATc                                                                                                                                                                                                                    <  2:322388/251‑1 (MQ=255)                            aaCGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATcga                                                                                                                                                                                                                  <  1:534652/251‑1 (MQ=255)                                  aaTTGCGATGCCGAAAGGTGCAGCGTGTTGGGGAATCCAGGAGAGGTCTTTTGCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACTATGAGGTCATCTATTACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCATATAACGCAAAAACTCCCGGGTGCTGCTGCCGCGAAGATCGACGATg                                                                                                                                                                                                             <  2:519216/250‑1 (MQ=255)                                   aTTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGt                                                                                                                                                                                                           <  1:439262/251‑1 (MQ=255)                                     ttCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTc                                                                                                                                                                                                          >  1:554907/1‑250 (MQ=255)                                       cGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCat                                                                                                                                                                                                        <  2:237857/250‑1 (MQ=255)                                            ccGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACTCCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGTTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGa                                                                                                                                                                                                  <  1:558693/251‑1 (MQ=255)                                             cGAAAGGTTCAGCGTGTTGGGCAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGCGTTAACACCGAGGCGGAAGAAATCTTCAGTCAAGTAGTAGCCGATGAGGTCATCTATCACACCGCCAGAGGCATTCAAACTCCCCGGGTAAAGGGCTTTGCCGCTTTTGGTGCGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGCAGATCGACGATGGTCATATGTGa                                                                                                                                                                                                  >  2:384467/1‑250 (MQ=255)                                               aaaGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGacac                                                                                                                                                                                               >  1:426400/1‑251 (MQ=255)                                                aaGGTTTAGCCTGTTGGGAACTCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGCGACGGAAGAACTCTTCAGCAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAGAGGGCTATGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCGGCCGCGACGATCGACGATGGTCATAAGTGacac                                                                                                                                                                                               >  2:561722/1‑250 (MQ=255)                                                   ggtCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCa                                                                                                                                                                                           <  2:520242/249‑1 (MQ=255)                                                      cAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAAc                                                                                                                                                                                        <  1:529444/251‑1 (MQ=255)                                                           gtttGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGATTTAACAACGAGGCGGAAGAAATCTGCAGTAAAGTAGGAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGCCACATCAAACATTcc                                                                                                                                                                                   <  2:464167/249‑1 (MQ=255)                                                                 gTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATc                                                                                                                                                                             >  1:120393/1‑251 (MQ=255)                                                                  tAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGACGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCg                                                                                                                                                                            >  1:179518/1‑251 (MQ=255)                                                                  tAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCg                                                                                                                                                                            >  1:561852/1‑251 (MQ=255)                                                                      ccAGTAGAGGGCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGGCGATGGTCATATGTGACACATCAAACATTCCGGCATCGgtac                                                                                                                                                                        <  1:20945/251‑1 (MQ=255)                                                                      ccAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGTTCATATGTGACACATCAAACATTCCGGCAGCGgta                                                                                                                                                                         <  1:124721/250‑1 (MQ=255)                                                                      ccAGGAGAGGTCTTTGTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCTGCGGCCGCGAATATCGACGATGGGCAGATGGGACACATCAAACATTCCGGCATCGgtac                                                                                                                                                                        <  2:297334/251‑1 (MQ=255)                                                                        aGGAGAGGTCTTTTTCGCGGGTGGCGGACTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGGAGACGATGAGGTCATCTATCACACCGCCAAAGGCATTCAACATCCCCGAGTAAAGGTCTTTGACGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATACCGCAAAAACTCCCGGGGGCGGCTGCCGCGAAGATCGACGATGGTCATACGTGACACATCACACATTCCGGCATCGgtacgt                                                                                                                                                                      >  2:58732/1‑251 (MQ=255)                                                                           agagGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGGACGTAcc                                                                                                                                                                   <  2:373208/251‑1 (MQ=255)                                                                               gTCTTGTTCGCGGGTGGCGGAGTTAACACCGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGCTAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAACCATTCCGGCATCGGTACGTACCGCat                                                                                                                                                               >  2:578622/1‑251 (MQ=255)                                                                                       cgcgGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCTCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATAGGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTtc                                                                                                                                                        <  1:234346/250‑1 (MQ=255)                                                                                       cgcgGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGCCACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTtcg                                                                                                                                                       >  2:437171/1‑251 (MQ=255)                                                                                       cgcgGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTtc                                                                                                                                                        <  2:488688/250‑1 (MQ=255)                                                                                          gggTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCTCATGATGTgcgtcg                                                                                                                                                    <  1:475393/251‑1 (MQ=255)                                                                                              ggCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGAttt                                                                                                                                                <  1:66816/251‑1 (MQ=255)                                                                                                       aacaacGAGGCGGAAGAAACCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCTCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCg                                                                                                                                        <  2:540026/250‑1 (MQ=255)                                                                                                        acaacGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCTCACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGAGCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTa                                                                                                                                      <  1:293333/251‑1 (MQ=255)                                                                                                           acGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCg                                                                                                                                        >  2:154212/1‑246 (MQ=255)                                                                                                           acGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTGCGTCGATTTGCGAACCg                                                                                                                                        <  1:154212/246‑1 (MQ=255)                                                                                                                 cGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCgg                                                                                                                             >  2:487736/1‑251 (MQ=255)                                                                                                                       aaaTCTTCAGTAAAGTAGTAGGCGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGcatcat                                                                                                                       <  2:426400/251‑1 (MQ=255)                                                                                                                             tCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCa                                                                                                                    <  2:443790/248‑1 (MQ=255)                                                                                                                             tCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGc                                                                                                                  <  2:108195/250‑1 (MQ=255)                                                                                                                              cAGTAAAGTAGTAGACGATGAGGTCATCTATCACACAGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAACAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGCTGGACATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGTTTTACGACACGTAATGCAGCTGCATCATCCAGCCg                                                                                                                >  2:195/1‑251 (MQ=255)                                                                                                                               aGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCCCATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGt                                                                                                               <  2:18396/251‑1 (MQ=255)                                                                                                                                ctAAAGAAGTAGACGATGAGGACATCTATCACACCGCCAGAGGCATTCAACATCACCGCGTAAATGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATACCGCACCAACTCCCGGGGGCGGCTGCCGAGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTCCGTACCGCATATTGGTCGTCGCTTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTg                                                                                                              >  2:80950/2‑251 (MQ=255)                                                                                                                                     gtagtaGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAAt                                                                                                         >  2:458970/1‑251 (MQ=255)                                                                                                                                       agtagACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATc                                                                                                        <  2:140708/250‑1 (MQ=255)                                                                                                                                        gtagACGATGAGGTCCTCTATCACACCGCCAGAGGCATTCAACATCCACAAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTAGCCAGCAGATAACGCAAACACTCCCGGGTGCGGATGCCGCGAAGATCGACGATGGTCATATTTGACAAATCAAACATTCCGGCATCGGTACGTCCCGCATGATGTTCGTCGTTTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGGGGAAATca                                                                                                       >  2:417345/1‑249 (MQ=255)                                                                                                                                           gACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATccacca                                                                                                   <  1:372410/251‑1 (MQ=255)                                                                                                                                           gACGATGAGGTCAACTATCACCCCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTGCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATccacca                                                                                                   <  2:725/251‑1 (MQ=255)                                                                                                                                                      cATCTGTCACACCGCCAGAAGCATTCAGCATCCCCGAGTACCGGGCTTTCCCGCTCTTGATGAGCTTCGCCACATCGTTCGCCTGAAGCTCACGCCCCAACTCCCGGGTGCGGCTGCCGAGACGATCGACGACGGACATATGTGACACATCAAACATTCCGGCATCGGTACGTCCCGCATGCTGTTCGTCGCTTTGCGAACCGTCATGCAGCGGCATCATACAGCCGTGGAAATCACCCATGCGAGcgccg                                                                                        >  2:585134/1‑251 (MQ=255)                                                                                                                                                           aTCACACCTCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGGACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCaaa                                                                                    <  1:81086/250‑1 (MQ=255)                                                                                                                                                                   gCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGtgtt                                                                           >  1:509581/1‑251 (MQ=255)                                                                                                                                                                    ccAGAGGCATTCAACATCCCCGAGTAAAGGGCTTGGCCGCTTTGGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACGCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGtgttg                                                                          <  1:578622/251‑1 (MQ=255)                                                                                                                                                                    ccAGAGGCATTCAACAGCACCGAGTAAAGGGATTTGCCGATTTTGGGGAGCTTCGCCACATCGTTCGACAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGGCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATTGGTACGTACCGCATGATGTTCGGCGATGTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCTCCATGCGAGCGCCGCAAAGCGTGtgttg                                                                          <  1:307251/251‑1 (MQ=255)                                                                                                                                                                       gagGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCAAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCACAGCGTGTGTTGTTc                                                                       >  2:330934/1‑251 (MQ=255)                                                                                                                                                                          gCATTCAACAACCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACAGTCCTGCATCGGTACGTACCGCATGATGTTAGTCGATTTGCGAACCGTAATGCAGCGGCATCAGCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTa                                                                    <  2:117711/251‑1 (MQ=255)                                                                                                                                                                               cAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACaaa                                                                <  1:487736/250‑1 (MQ=255)                                                                                                                                                                                  cATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAg                                                            <  1:60752/251‑1 (MQ=255)                                                                                                                                                                                     ccccGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACCTCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGATCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCt                                                         <  2:158464/251‑1 (MQ=255)                                                                                                                                                                                      cccgagcgaaGGGCTTTGACGCTATTGGAGACCTCCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGCTCGACGCTGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAACGCGTGTGTTGTTCGTCCAAAGGAGTCtg                                                        >  2:511589/9‑251 (MQ=255)                                                                                                                                                                                       ccGAGTAAATGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAAGCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCtgt                                                       <  2:482341/251‑1 (MQ=255)                                                                                                                                                                                            tAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGc                                                  >  1:489628/1‑251 (MQ=255)                                                                                                                                                                                                           cTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAATGAGTCTGTTGTGCCATC‑TTGTCATCAtt                                  >  2:329064/1‑251 (MQ=255)                                                                                                                                                                                                                    aGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATC‑TTGTCCTCATTGAATAAGCg                         <  1:529259/251‑1 (MQ=255)                                                                                                                                                                                                                       ttCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGCAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATC‑TTGTCCTCATTGAATAAGCgggg                      >  2:545995/1‑251 (MQ=255)                                                                                                                                                                                                                         cGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATC‑TTGTCCTCATTGAATAAGCGGGGCt                    <  2:127971/251‑1 (MQ=255)                                                                                                                                                                                                                            cacaTCGTTCGCCAGCAGACAACGCAACAACTCCCGGGTGCGGATGCCGCGCCGATAGACGATGGTCATATGTGACAAATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCATCGCTTTGCGAACCGAAATGCAGCGGCATCATCCAGCAGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGACATC‑TTGTCCTCATTGAATAAGCGGGGCTGAc                 >  2:588223/1‑251 (MQ=255)                                                                                                                                                                                                                                 cGTTCGCCAGCAGAAAACGGAAAAACCCCCGGGCGCGGCTGCTGCGAATATCGACGATGGTCACAGGTGACACATCAAACCTTCCGGCATCGGTACGTGACGCATGCTGTTCGTCGATTTGCGATCCGTAATGCAGCTGCATCATCCAGACGTGGAAATCCACCATGCGAGCGCCGCAAATCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCGTGGTCC‑CATTAAATAAGCGGGGCTGACAACt             <  2:333366/250‑1 (MQ=255)                                                                                                                                                                                                                                        cagcagATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATC‑TTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATg     >  2:111324/1‑251 (MQ=255)                                                                                                                                                                                                                                          gcagATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATC‑TTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGt   <  1:545995/251‑1 (MQ=255)                                                                                                                                                                                                                                            agATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATC‑TTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTg  >  1:203462/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 CACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATC‑TTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTG  >  NC_000913/3050227‑3050711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑