Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,772	C→A	G155G (GGC→GGA)	ppnN →	nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,772	0	C	A	100.0%	194.2 / NA	61	G155G (GGC→GGA)	ppnN	nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/31);  total (30/31)

AGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATC  >  NC_000913/2926525‑2927009                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGt                                                                                                                                                                                                                                            <  2:121302/251‑1 (MQ=255)   cTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTc                                                                                                                                                                                                                                           >  1:429924/1‑250 (MQ=255)       aaCTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTc                                                                                                                                                                                                                                       >  2:20663/1‑250 (MQ=255)           gATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAAtt                                                                                                                                                                                                                                   <  1:20663/250‑1 (MQ=255)               aatcttccGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACg                                                                                                                                                                                                                               <  2:484226/244‑1 (MQ=255)                  cttccGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAAAGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGaaa                                                                                                                                                                                                                            >  1:429341/4‑250 (MQ=255)                         aaGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTATCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCAAATCACTACCCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTAt                                                                                                                                                                                                                    >  1:579406/1‑251 (MQ=255)                              gCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTa                                                                                                                                                                                                               <  1:393805/251‑1 (MQ=255)                                      cGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTTACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtc                                                                                                                                                                                                       >  2:425620/1‑251 (MQ=255)                                                        cgcTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTgg                                                                                                                                                                                     <  2:552886/251‑1 (MQ=255)                                                                 ggCCAACTTGTACGCGGCCCTGCGGGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGACTCTCGACTCCTCCGTCCCCATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCACATCTGGCGGTCTGCTGGGGAGGTCACTCACTTCACGAAAACGAGTATTTCTATGCCCCGCGCGTCGCAAACCAGCTGGGCCTGCg                                                                                                                                                                              >  2:409839/1‑249 (MQ=255)                                                                    cAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGc                                                                                                                                                                         >  1:115387/1‑251 (MQ=255)                                                                      aCTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTg                                                                                                                                                                       <  2:269659/251‑1 (MQ=255)                                                                               cgGTCATGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTACGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAAAGCTCGCGCGCTGCATGTGGGTGAAGCGACAAATATGGTGGTCTGCTGGGGAGGTCAATCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGATGGGCCTGCGTGAGCTGAATATCTGc                                                                                                                                                              >  2:535726/1‑251 (MQ=255)                                                                                gggccTGCTTGACATCCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTTGGGAGGTCACTCAATTAACGAAAACGAGTAGTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCa                                                                                                                                                             <  2:379325/248‑1 (MQ=255)                                                                                         tGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCtgtg                                                                                                                                                    >  2:15911/1‑251 (MQ=255)                                                                                                  ctTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCggg                                                                                                                                             >  1:476751/1‑249 (MQ=255)                                                                                                     cGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGAAAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTCTTTGTATGCCCGTCGCGTCGGAACCCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGAGAGCg                                                                                                                                         >  1:296783/1‑250 (MQ=255)                                                                                                     cGTTTACGGGCAAAGCCATAACACCGTTCGTTTGCCCACCCTGAATCTCGACAACGCCGACCACATCACTAACCTGGTCTGTTCCATCTTGCGGAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAAGATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCCCCGGCTGGGGTCCGGGAGCg                                                                                                                                         <  2:489244/250‑1 (MQ=255)                                                                                                      gTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGAt                                                                                                                                       >  2:161273/1‑251 (MQ=255)                                                                                                      gTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGAt                                                                                                                                       <  1:105630/251‑1 (MQ=255)                                                                                                         tACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATgc                                                                                                                                     <  1:425620/250‑2 (MQ=255)                                                                                                                   tCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCgctg                                                                                                                          >  1:194631/1‑248 (MQ=255)                                                                                                                            ccGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGtgct                                                                                                                 <  1:183880/251‑1 (MQ=255)                                                                                                                            ccGTTCGTTTTCCCAACATGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGATCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGCAAGGtgct                                                                                                                 >  1:397520/1‑251 (MQ=255)                                                                                                                                 cgttTTCCCAACCTGAATATCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATCTCTGCACCGGCTGTAGTCCGGGAGCGATGGAAGCGCCGAAGAAAGGtgacgcggg                                                                                                            >  2:538357/1‑244 (MQ=255)                                                                                                                                  gttTTCCCAACCTGAATCTCGACACCTCCGTCAACATCACTAAACTGGCCTTTTCAATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCCACTATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGCAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGCGCGATGGAAGCGCCGATGAAGGGTGCTGCgg                                                                                                             >  2:579797/1‑249 (MQ=255)                                                                                                                                   ttttCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGTTGCGGTc                                                                                                           <  1:415685/250‑1 (MQ=255)                                                                                                                                    ttgcccAACCTGAATCTCGCCAACTCCGGCCACATCTCTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATTTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGGCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCGCCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCg                                                                                                          <  2:444216/247‑1 (MQ=255)                                                                                                                                        ccAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGacac                                                                                                     >  1:537535/1‑251 (MQ=255)                                                                                                                                              tGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACgcgc                                                                                                 >  2:264445/1‑249 (MQ=255)                                                                                                                                               gAATCTCGACAACTCCGTCCACATCACTACCCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGGTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCgcag                                                                                               <  2:560906/250‑1 (MQ=255)                                                                                                                                                     cGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGTCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGcagcag                                                                                            <  2:38623/247‑1 (MQ=255)                                                                                                                                                     cGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGtt                                                                                        >  2:173288/1‑251 (MQ=255)                                                                                                                                                       aCAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTAc                                                                                      >  1:204780/1‑251 (MQ=255)                                                                                                                                                        cAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTAc                                                                                      >  2:294650/1‑250 (MQ=255)                                                                                                                                                         aaCTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACaa                                                                                    <  2:115387/251‑1 (MQ=255)                                                                                                                                                             ccGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCATCAGCGTTACAAAGAc                                                                                <  1:446935/251‑1 (MQ=255)                                                                                                                                                               gTCCACATCACTAACCTGGTCTTTTCCAGCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAg                                                                              <  2:315448/251‑1 (MQ=255)                                                                                                                                                                       cACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcg                                                                                                                                                                                                      <  2:195057/123‑1 (MQ=255)                                                                                                                                                                        aCTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTAt                                                                     >  1:206012/1‑251 (MQ=255)                                                                                                                                                                         cTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTAtt                                                                    >  1:365483/1‑251 (MQ=255)                                                                                                                                                                             ccTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTTCTTCGTTCGGACACGCGCAGCATCGTTTCAAAGACCGTCGTTTTATTg                                                                   <  2:398557/248‑1 (MQ=255)                                                                                                                                                                             ccTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCAGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGACGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTg                                                                   >  1:398557/1‑248 (MQ=255)                                                                                                                                                                                ggTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGACAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTAt                                                               <  2:137209/249‑1 (MQ=255)                                                                                                                                                                                     tttCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTTGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACag                                                          <  2:429924/249‑1 (MQ=255)                                                                                                                                                                                        ccATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCg                                                     >  1:372199/1‑251 (MQ=255)                                                                                                                                                                                              tGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTc                                                   <  1:391655/247‑1 (MQ=255)                                                                                                                                                                                               gCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGAtt                                               <  2:204780/250‑1 (MQ=255)                                                                                                                                                                                                 gTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCAGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGCTGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATc                                            >  2:474765/1‑251 (MQ=255)                                                                                                                                                                                                   aaCGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATcgc                                          <  1:423675/251‑1 (MQ=255)                                                                                                                                                                                                      gCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGATGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGCCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGATGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATcgccgc                                       >  1:564993/1‑251 (MQ=255)                                                                                                                                                                                                       cgcgcgcgCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGGGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGTGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCt                                      <  1:82279/249‑1 (MQ=255)                                                                                                                                                                                                             cgcTGCATGTGGGGGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGATTATTTGTATGCCCGTCGCGGCGGAAACCAGCGGGGCCTGCGTGAGCTGAATATCGGCACCGGCGGTGGTCCGGGATCTATGGAAGCTCCGATGAAAGGGGCGGCGGTCGTACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCCTCGATTATCGCCGCTGAACCg                                <  2:579406/251‑1 (MQ=255)                                                                                                                                                                                                                  cATGTCGGTGAAGCGCCAAATATGGTGGACTGCTGGGGAGGTCGCTCAATTAACGAAAACGAGTATTTGTATGACCGTCGAGTCGGAAACCAGCTGGGCCACCGCGAGCTGAATATCTGCCCCGGCTGTGGTCCGGGCGCGATGGAAGCCCCGATGCAAAGTGCTGCGGTCGGCCACGCGCAGCAGCGTTACAAAGACAGTCGATTTATTGGTCTGCCAGAGCCGTCGTTTCTCGCCGCTGAACCGCCTac                           >  2:303289/1‑250 (MQ=255)                                                                                                                                                                                                                          ggaatcgcCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGGCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGTTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTgg                   <  1:403292/246‑1 (MQ=255)                                                                                                                                                                                                                            aaGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTc                 <  1:173288/251‑1 (MQ=255)                                                                                                                                                                                                                             aGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCa                <  1:215482/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaaTATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGAATGCCCGTCGCGTCGGAAACCAGATGGGCCTGCGTGAGCTGAATATCTGCAACGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCACCGAAt          >  1:549833/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggtggtCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGGCGGAATCCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGatta    <  2:362230/251‑3 (MQ=255)                                                                                                                                                                                                                                            ggtCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGCCAACGAATTGatcatc  >  1:115963/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              AGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATC  >  NC_000913/2926525‑2927009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑