Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,789,286	G→A	G101D (GGT→GAT)	glaH →	glutarate dioxygenase GlaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,789,286	0	G	A	97.1%	219.4 / NA	68	G101D (GGT→GAT)	glaH	glutarate dioxygenase GlaH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (40/26);  minor base C (2/0);  total (42/26)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGCAAAACAT  >  NC_000913/2789042‑2789532                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ggATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCg                                                                                                                                                                                                                                                   >  1:261780/1‑250 (MQ=255)  gATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCgat                                                                                                                                                                                                                                                 <  1:275416/251‑1 (MQ=255)        ccctcacccCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGCtgtg                                                                                                                                                                                                                                           >  1:575840/1‑251 (MQ=255)                ccGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGaagcaagc                                                                                                                                                                                                                                   >  1:534409/1‑251 (MQ=255)                  gTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGAAGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGCACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGACGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCgg                                                                                                                                                                                                                                 >  1:95948/1‑251 (MQ=255)                          aaTCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACCGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGCTGATGTGAAGCAAGCGGATGAGATg                                                                                                                                                                                                                         >  1:480979/1‑251 (MQ=255)                                         tgGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGAcg                                                                                                                                                                                                          >  1:263912/1‑251 (MQ=255)                                                 aCCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGgc                                                                                                                                                                                                  >  1:109198/1‑251 (MQ=255)                                                          gAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGAt                                                                                                                                                                                         >  1:448176/1‑251 (MQ=255)                                                             cAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTgg                                                                                                                                                                                      <  1:297290/251‑1 (MQ=255)                                                                acgacCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCg                                                                                                                                                                                   >  1:440227/1‑251 (MQ=255)                                                                    ccAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTcc                                                                                                                                                                               >  2:475577/1‑251 (MQ=255)                                                                    ccAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCAAAAATTCTTGCCGATCTATGTGCGAACCCGCTGCAACCATTGCTGTTGCAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTcc                                                                                                                                                                               >  1:361047/1‑251 (MQ=255)                                                                      aaaCAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGAGGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCaa                                                                                                                                                                             <  1:272026/251‑1 (MQ=255)                                                                       aaCAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAAt                                                                                                                                                                            >  1:267887/1‑251 (MQ=255)                                                                        aCAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCCTCCGATTTGCCGGTCCAAtt                                                                                                                                                                           >  2:208347/1‑251 (MQ=255)                                                                           gTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTcg                                                                                                                                                                        >  2:215343/1‑251 (MQ=255)                                                                             ttCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTcagg                                                                                                                                                                      >  2:439140/1‑248 (MQ=255)                                                                             ttCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTc                                                                                                                                                                         <  1:439140/248‑1 (MQ=255)                                                                                     cAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGATTACATATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAg                                                                                                                                                              <  1:374160/251‑1 (MQ=255)                                                                                     cAGGGTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGATAAACAGAGCTGAAGGAGCGTTGTTGTTCAATGCGGAGGATGTAGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGTGCATCTGATTGGACTTTCCAATTTcgttgtgaag                                                                                                                                                                >  1:537715/1‑241 (MQ=255)                                                                                             cGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGt                                                                                                                                                      >  1:138871/1‑251 (MQ=255)                                                                                                       gTGCAAGCGCTGGAGTACAAATCGTTTCAGCGTTTTCGGGTAGCCAAAGTTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGGGGCGCCTCTGATTGGCCGTTCCAATTTTGATGCGCTGAGTGGTCAGTATTAcgcgcg                                                                                                                                            >  2:548182/1‑251 (MQ=255)                                                                                                          cAGGAGCTTGAGTACAAATCGGTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGAGAACCAGCTGCAACCATTGCTGTTGAAGCCGTTGCTCAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGAAGTCGATGATGTGAAGCAAGCGGATGAGATGGTTAAGCTGGCGACGGCGGTGGCGCATCAGATTGGCCGTTCCAATTTCGATGCGTTGAGCTGTATGCATTACGCGCGttt                                                                                                                                         >  2:448786/1‑251 (MQ=255)                                                                                                           aGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGAGAAGCTGGCGACGGCGGTGGCGCATCTGATTTGCCGTTCCAATTTCGGTGCGGTGAGCGGTCAGTATTACGCGCGtttt                                                                                                                                        >  1:255991/1‑251 (MQ=255)                                                                                                            ggCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTg                                                                                                                                       <  1:110565/251‑1 (MQ=255)                                                                                                               gcTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAg                                                                                                                                    <  1:289390/251‑1 (MQ=255)                                                                                                               gcTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAg                                                                                                                                    <  2:138871/251‑1 (MQ=255)                                                                                                                  ggAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGt                                                                                                                                   <  1:204054/249‑1 (MQ=255)                                                                                                                  ggAGTACAAATCGTTTCTGCGTTTTCGGGTAGCAAAAATTCTTGCCGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCCTCTGATTGGCCGGTCCAATTTCGATGCGATGAGCGGTCAGAATTACGCGCGTTTTGTAGTg                                                                                                                                  >  2:427926/1‑250 (MQ=255)                                                                                                                   gAGTAAAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAACCCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGa                                                                                                                                 >  1:355766/1‑250 (MQ=255)                                                                                                                          aaTCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGGGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTAAGCGCGTTTTGTAGTGAAAAATGTc                                                                                                                         >  2:447441/1‑251 (MQ=255)                                                                                                                           aTCGTTTCTGCGTTTGCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTGTGGTAGTGAAAAATGTCg                                                                                                                        <  2:109198/251‑1 (MQ=255)                                                                                                                             cGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCTTTTTGTAGTGAAAAATGTCGa                                                                                                                       <  2:579439/250‑1 (MQ=255)                                                                                                                              gTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGCTCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGCAGGTGCGTTGTTGATCAATGCGGTGGCTGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGAt                                                                                                                      >  2:349198/1‑250 (MQ=255)                                                                                                                                   tGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGGGCGGTCAGTAt                                                                                                                                                    >  2:353781/1‑215 (MQ=255)                                                                                                                                   tGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTAt                                                                                                                                                    <  1:353781/215‑1 (MQ=255)                                                                                                                                   tGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCAGCAACCATTGCTGTTGAAGACGTTACTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGAAGGAGGGGGCGCAACTGATTGGCCGTTCCAATTTCGATGCGATGCGCGGTCAATATTACGCGAGTTTTGTAGTGAAAAATGTCGATAACTc                                                                                                                 >  1:398681/1‑250 (MQ=255)                                                                                                                                         ttCGGTTAGCCAACATTCCTGACGATCTCTGTGCGAACCAGCTGCAACCATTGCTGTTGAAGCCGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGAGGTGGATGTCGATGATGTGAAGCCAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCCATTTCGCTGCGATGAGCGGCCAGTATTACGCGCGTTTTGTAGTGAAAAATGGCGATAACTCAGAc                                                                                                             >  2:546517/1‑248 (MQ=255)                                                                                                                                         ttCGGGTAGCCAAAATTCTGGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCTATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATATCTCAGACAg                                                                                                           <  2:39068/250‑1 (MQ=255)                                                                                                                                           cGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGGTTTGTAGTGAAAAATGTCGCTAACTCAGACAGCt                                                                                                         >  2:330287/1‑250 (MQ=255)                                                                                                                                           cGGGTAGCCAAAATTCTTGAAGATCTATGCGCGAACCAGCTGACACCCTTGCTGTTGAAGCCGTTGCTAAACCGCGCTGACGGTGCGTTGTTGCTCAATGCGGTGGATGTCGATGATGTGCAGCCAGCGGATGAGATGGTGAAGCTGGCGCCGGCGGTGGCGCCTCTGATTGGCCGCTCCCATTTCGATGCGATGGGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTAATACAGCt                                                                                                         >  2:139494/1‑250 (MQ=255)                                                                                                                                                   cAAAATGCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCAGCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGcc                                                                                                <  1:546517/251‑1 (MQ=255)                                                                                                                                                        ttCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCg                                                                                           >  1:149782/1‑251 (MQ=255)                                                                                                                                                              aCGAGCTATGTGCGAACCAGCTGCAACCCTTGATGTTGAAGCCGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGAAGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCAcagc                                                                                     >  2:346421/1‑248 (MQ=255)                                                                                                                                                                  tCTATGTGCGAACAAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCCGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCACCCGCACcgcggc                                                                                  >  2:301523/1‑248 (MQ=255)                                                                                                                                                                           gAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTac                                                                        >  1:349477/1‑251 (MQ=255)                                                                                                                                                                                 gCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAacg                                                                  >  2:48929/1‑251 (MQ=255)                                                                                                                                                                                    gCAACCATTTCTGTTGATAACGTTGCTAAACCGCGCTGAAGGGGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAacgac                                                                <  1:338133/250‑1 (MQ=255)                                                                                                                                                                                     cAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGAGGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGGCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACgg                                                              <  2:583843/251‑1 (MQ=255)                                                                                                                                                                                         cATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACg                                                          >  1:289506/1‑251 (MQ=255)                                                                                                                                                                                             gCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATg                                                      <  1:456214/251‑1 (MQ=255)                                                                                                                                                                                               tGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTc                                                    >  1:95111/1‑251 (MQ=255)                                                                                                                                                                                                     aGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAagag                                              <  2:440300/251‑1 (MQ=255)                                                                                                                                                                                                          ttGCTAAACCGGGCTGAAGCTGCGTTGTTGATCACTGCGGTGGATGTAGATGATGTGAAGCACGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCAGCACcgcg                                                                                    >  2:537588/1‑208 (MQ=255)                                                                                                                                                                                                          ttGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACGCAGACAGCTATCTGCGCCAGCCGCACcgcg                                                                                    <  1:537588/208‑1 (MQ=255)                                                                                                                                                                                                               aaaCCGCGCTGAAGGTGCGTTGTTTATCAATGCGGTGGATGTCGATGAGGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGAGGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGAt                                     <  2:480979/250‑1 (MQ=255)                                                                                                                                                                                                                 aCCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTAc                                  >  1:380601/1‑251 (MQ=255)                                                                                                                                                                                                                   cgcgCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGCAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGCTAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGt                                >  2:193680/1‑251 (MQ=255)                                                                                                                                                                                                                     cgcTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATCACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGtac                              >  1:394124/1‑249 (MQ=255)                                                                                                                                                                                                                      gcTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCGACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCt                             <  2:575840/251‑1 (MQ=255)                                                                                                                                                                                                                       cTGAAGGTGCGTTGTTGATCAATGCGGTGGCTGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGTGATGAGCCGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATCACTCAGACAGCTATCTGCGACAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGGAGGGATAAACGCTTACGTGCtg                            >  1:274984/1‑251 (MQ=255)                                                                                                                                                                                                                        tGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAg                                                                                                                                    >  2:18589/1‑146 (MQ=255)                                                                                                                                                                                                                        tGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAg                                                                                                                                    <  1:18589/146‑1 (MQ=255)                                                                                                                                                                                                                         gAAGGTGCGTTGTTGATCAATGAGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGTGGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTTCACAACGACGGCACGTATGTCGAATAGATAACCGATTACGTGCtg                            <  2:557221/249‑1 (MQ=255)                                                                                                                                                                                                                         gAAGGTGCGTTGTTGAGCCATTCGGTGGATGTCGGTGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTTGCGCATCTGATTGGCCTTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGGCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGtgatga                           <  2:575096/250‑1 (MQ=255)                                                                                                                                                                                                                                    gttgATCAATGCGGTGGATGTCGGTGATGTGAAGCACGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGGCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGTTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGATATAACCGATTACGTGCTGATGATGAAAATcg               <  2:95948/251‑1 (MQ=255)                                                                                                                                                                                                                                          cAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAg          <  1:400281/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gtggatgTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGCAAAACAt  <  1:330287/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGCAAAACAT  >  NC_000913/2789042‑2789532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑