Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,410,419	G→A	L312L (CTG→TTG)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,410,419	0	G	A	97.8%	140.6 / NA	45	L312L (CTG→TTG)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/19);  minor base C (1/0);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CCAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCG  >  NC_000913/2410176‑2410664                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ccAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATc                                                                                                                                                                                                                                                >  1:226143/1‑251 (MQ=255)   cgCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCgg                                                                                                                                                                                                                                              >  2:108699/2‑251 (MQ=255)    gCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGc                                                                                                                                                                                                                                             >  2:575954/1‑251 (MQ=255)          cggtgaggagccAGTTACCCGCAACCAGGATGATATCGCCCGGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCGTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCGGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCGGCGCAATACCATCGGCTCCAGc                                                                                                                                                                                                                                       <  2:248403/241‑1 (MQ=255)                    ccAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGTCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATGTCGCTTTAGCCCCACCACATTCATTCCGTAGCGGTTACGAAAACCAATTTCGCGCACCGATT‑ACCAATCAGTTCTGACGCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACt                                                                                                                                                                                                                            <  1:210009/251‑1 (MQ=255)                       gTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGAGAACCTTCCAGCGCCACGCAATTGCGCTATAGCCCAACCACATTCAGTCCGTAGCGGGTACGACAACCAATTTCGCGCAACGATTTACCAATAAGTTCTGCCTCAGGAATTAATGAAATCTCTGCCATGCCCAAATCAAGGGACTGGTAAGTAAAATACTCGCAGCGCAATACCATCGGCTCCAGCAATTGCTCACTAc                                                                                                                                                                                                                          >  1:574818/1‑251 (MQ=255)                            ccACAACCAGGCTGATATCGCCCAGCAGCAGAGGCTCATCCGCCCGCGAACCTTCCCGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAACACCAATTTCGCGCACCGATTTACCACTCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAAt                                                                                                                                                                                                                     >  2:303222/1‑251 (MQ=255)                               caACCAGGACGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGc                                                                                                                                                                                                                  >  1:463006/1‑251 (MQ=255)                                    aGGATGCTATCGCCACGCAGCCGAGGCTAATGCGCCAGTGAAAATACCAACGCAAAGCAATTGCGCTTTAGCCCCACCAACTTCAGTACGTAGCGGGTACGAAAACAAATTTCGCGCACCGATTTACCAATCAGTTCTGACGAAGGAATAAATGAAAAAGCTGACACGCGAACAACAAGGGCCGGGTCAGAACAAAACTAGCGGCGAAATAACATCGGCCCCAGCaagagacaacaaaaacatcgccgg                                                                                                                                                                                                               >  2:389591/1‑227 (MQ=255)                                     ggATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCgg                                                                                                                                                                                                               >  2:442059/1‑248 (MQ=255)                                        tgatATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCgg                                                                                                                                                                                                               >  1:325245/1‑245 (MQ=255)                                              cGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTGTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATc                                                                                                                                                                                                    <  1:474785/250‑1 (MQ=255)                                                cccAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGACGGAGATCGACATCAGc                                                                                                                                                                                                 >  1:247652/1‑251 (MQ=255)                                                     ccgaggagGCCCAGCCGCCAGCGAACCTTCCAGCGCCACGCCAGTGCACTTTAGCCCCACCACATCCAGGCCGTAGCGGGTACGAAAACCAATTTCGCGCACCTATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCGGCCAGGCCCTCATCAAGGTCCTGGTCAGAAAAATGCTCGCCGCGCAATACCATCGGCTCCAGCAAGGGCTGACGGGAAAATAGCCTGAGATCGGCTTCAGCCGCAg                                                                                                                                                                                            <  2:57470/246‑1 (MQ=255)                                                          gagGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACata                                                                                                                                                                                       <  1:442059/251‑1 (MQ=255)                                                                cATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCaa                                                                                                                                                                                   <  1:113011/249‑1 (MQ=255)                                                                 aTCCGCCAGCGAAACTTCCAGCGCAACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTACAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATa                                                                                                                                                                                 >  2:435765/1‑250 (MQ=255)                                                                                 tCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAcgcg                                                                                                                                                                 <  1:575954/250‑1 (MQ=255)                                                                                   cAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACAATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAcgcgcgc                                                                                                                                                              >  1:393842/1‑251 (MQ=255)                                                                                                        tAGCACCACCACATTCAGTCCGTAGCGGGTACGAAAACCTCTTTCGCGCACCGATTTACCACTCAGTTCTGCCTCAGGAATTAATGACCTCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCCATTGCTCACTCCAAAATTGCCGGCGATCGACATCAGCCGCAGCCCTCTCAATAAGACACACGTCCCGCGCTCGACATTAAGAAACACCAtaac                                                                                                                                         >  2:205608/1‑248 (MQ=255)                                                                                                                    aTTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATa                                                                                                                              >  1:253271/1‑250 (MQ=255)                                                                                                                           ccgtatctggTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATTCTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCTCGCGCGCGAAATTCAGAAACCCCATTATCGTTCACGATAACGcgac                                                                                                                       <  1:464008/242‑1 (MQ=255)                                                                                                                           ccGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCCAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGcgacg                                                                                                                      >  1:34835/1‑251 (MQ=255)                                                                                                                               aGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGaaaa                                                                                                                  <  1:311023/251‑1 (MQ=255)                                                                                                                                     tACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAAcgccgc                                                                                                            >  1:33847/1‑251 (MQ=255)                                                                                                                                                        cACCGATTTACCAATCCGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATg                                                                                          >  1:192756/1‑250 (MQ=255)                                                                                                                                                           cGATTTACCACTCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCACTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGCCATATCAATAAGCAAAACGTCACGCGCGCGAAAt                                                                                                                                                         >  2:382328/1‑184 (MQ=255)                                                                                                                                                           cGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAAt                                                                                                                                                         <  1:382328/184‑1 (MQ=255)                                                                                                                                                                     aTCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCAt                                                                             >  1:14600/1‑250 (MQ=255)                                                                                                                                                                       cAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATaa                                                                           <  1:500039/250‑1 (MQ=255)                                                                                                                                                                                   aGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCAGCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGGCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACGCCGATGACGTTATCGCCATAGCGCTCGCGTAAtt                                                              <  2:253271/251‑1 (MQ=255)                                                                                                                                                                                       aTTAATGAAATCTATGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATAATCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACACAATTGCCGGAGAACGACATCAGCAGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTAACGATAACGCGACGAAAACGCCGCCAGCGATCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTg                                                            >  2:378983/1‑249 (MQ=255)                                                                                                                                                                                          aaTGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGatc                                                       <  2:463006/251‑1 (MQ=255)                                                                                                                                                                                          aaTGAAATCGCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGGTAGCGCCATAACGCTCACGTGATTTGAGatc                                                       <  1:153930/251‑1 (MQ=255)                                                                                                                                                                                           aTGAAATCCCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGAGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGGTCAACACCGATGACGGTAGCGCCATAACGCTCACGTAATTTGAGatca                                                      <  2:357644/251‑1 (MQ=255)                                                                                                                                                                                                    ctGCCATGCCCACATCAAGTGCCTGGTCAGAAAAAAACTCGCCGCGCAATACCAGCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGACACGCGCGCGAAATTCAGAAACCCCATTAACGTTCGCGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGAGGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAg                                                 <  2:151288/247‑1 (MQ=255)                                                                                                                                                                                                         aTGCCCACAGCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCAGTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGAcc                                        <  2:14600/251‑1 (MQ=255)                                                                                                                                                                                                                  tCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACACAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAACGTGCAACACCGATGACGTTAGCGCACTAACGCTCACGTAATTTGAGATCAGCCAGCCGTTGACCAATCATTgg                               >  2:446851/1‑251 (MQ=255)                                                                                                                                                                                                                          cTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCgg                       >  2:55623/1‑251 (MQ=255)                                                                                                                                                                                                                          cTGGTCAGAAAAATACTCGCAGCGCAATACCATCGGCTCCAGCAATTGGACACTACCAAATTGCCGGAGATCGTCATCAGCCGCAGACATATCAATAAGCACAACGTCACGGGCGCGAAATTCCGAAAACCCATTAACGCTCACGATAACGCGACCAAAACGCCCACAGAGTTCAACACCGATGCCGTTACGGCCATAACGCTCACGTCATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGAACCgg                       >  1:586456/1‑251 (MQ=255)                                                                                                                                                                                                                            ggTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCTCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACTTAATTTGAGATCATCCAGCCGTTGACCAATCATTGTCGATCCgggg                     <  1:225054/251‑1 (MQ=255)                                                                                                                                                                                                                                aGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGa                  >  1:358599/1‑250 (MQ=255)                                                                                                                                                                                                                                        aCTCGCCGCGCAATACCATCGGCTCCAGCAATTGCCCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACAGATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGa         <  2:286284/251‑1 (MQ=255)                                                                                                                                                                                                                                              cgcgCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTcgc   >  2:553475/1‑251 (MQ=255)                                                                                                                                                                                                                                               gcgcAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTcgcg  >  2:582295/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      CCAGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCG  >  NC_000913/2410176‑2410664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑