Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,384,808 C→T K305K (AAG→AAA)  yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,384,8080CT93.5% 90.9 / ‑2.7 31K305K (AAG→AAA) yfbKIPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (16/13);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

ATTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTG  >  NC_000913/2384562‑2385046
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
aTTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTTGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTAGTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGCCCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCTCCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTtc‑g                                                                                                                                                                                                                                            <  1:565470/251‑1 (MQ=255)
  tCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTtc‑gtc                                                                                                                                                                                                                                          >  2:111203/1‑251 (MQ=255)
        cTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCctgc                                                                                                                                                                                                                                    >  2:261678/1‑251 (MQ=255)
             tGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGAt                                                                                                                                                                                                                                >  1:467764/1‑250 (MQ=255)
                                           gaGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAATCGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATGTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAAGAAACCCTCTC‑GTCGCCTGCGGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGt                                                                                                                                                                                                 <  2:68199/250‑1 (MQ=255)
                                            aGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTa                                                                                                                                                                                                <  1:557706/251‑1 (MQ=255)
                                                            aTCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGCTTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATccc                                                                                                                                                                                >  1:426356/1‑250 (MQ=255)
                                                              cGGCAATTCGCACCAGCATTGCCTCGTGGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACCCCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCTATTGAGGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAg                                                                                                                                                                               <  1:414059/250‑1 (MQ=255)
                                                               ggCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACCCCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAACCCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCg                                                                                                                                                                             <  1:127308/251‑1 (MQ=255)
                                                                                      tgTTGTAATTGCTATTCCCCCCGCCAAACGCCGACAGAGTAACCCCAGACCCCCGCTGTTTTTTGACCGTTGATTCAATCGTTTTTGGATCGTCAATGCCAACGTTAAATTAACCGTCAGTGGCTAATAAAATGCGATTGGTGCCCCCCTTAATAAACCCTTTC‑GTGGCCGGCTGATAAGCCGGTTCCAGCCCGGCACCGCTATTGGTACTGCTTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTc                                                                                                                                                       <  2:488022/249‑1 (MQ=255)
                                                                                       gTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACCCCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGGAAATGCCAACGTTAAAGGCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCGGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCg                                                                                                                                                     <  2:426356/251‑1 (MQ=255)
                                                                                        ttGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGc                                                                                                                                                    >  1:413673/1‑251 (MQ=255)
                                                                                          gtatttGCCATTCCCCACGCCAAACTGCGACAGAGTAACCCCAGACTCCGCCTGTTTTTTGACCATTGATCCATCCGATTTTGGATCGTCTATGCCAACTTTAAAGTCACCTTCAGTGCCTAATAAAATGCGATTAATGCCGCCCTTAATAC‑CCCCTTCCGTCTCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGTTACTGCCTTCGGCATCCAGCGAATCAATTGCTGTATCAATTACCGCCt                                                                                                                                                  <  2:71876/247‑1 (MQ=255)
                                                                                                                       aCAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATCCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGCCACCGTCAGTGTCTAATAAAATGCGATTGAGGCCGCCCTTAATAACCCCTTTC‑GTCGCCTGCTAATAAGCCAGTCCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATGTCCGCCTTATGACGTCCGGAGATAGAAGgcaatgca                                                                                                                     <  2:266854/251‑1 (MQ=255)
                                                                                                                         agagTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATCAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAAATAGAAGgcaatgcaat                                                                                                                   >  2:221073/1‑251 (MQ=255)
                                                                                                                                         ccggCTGTTTTTTGACCAATGATTCAATCGATTTTGGATCGTCAATTCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGGTTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTTATAAGCCAGTTCCAGCCCGGAACCGCCATTGGTACTTCCTACGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGc                                                                                                    <  1:573572/247‑1 (MQ=255)
                                                                                                                                             cTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAgg                                                                                               >  1:182636/1‑251 (MQ=255)
                                                                                                                                                     tGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCTCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGAt                                                                                        <  1:63170/250‑1 (MQ=255)
                                                                                                                                                           ttGATTCACTCGAATTTCGAAAGTCAAAGCCCACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGACGCCCCCCTTAATAAACACTTTC‑GTAGCCTGATGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGACTTATTACTCCCGGACATAGACGGCAATGCAATACGGGAGTCGCCAGCCTAGGTCACGATGGCAATg                                                                                 >  2:349167/1‑251 (MQ=255)
                                                                                                                                                                     cgtttttGGATCTTAAACGCCATAGTTAAAGGCACCGTCAGGGGCTAATAAAAGGCGATTGAGGCCGCCCTGAATAA‑CCCCTTCCGTCGCCTGCGGAAAAGCTAGTGCCAGGCCGGCACCGCCATTGGTGCTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGAGAGAAGGCAATGCATGACGGTAGTCGCCAGTGTAGGTCTCGATGGCATTGTTATCCTGCt                                                                       <  2:578543/248‑1 (MQ=255)
                                                                                                                                                                     cGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCt                                                                       <  1:215340/251‑1 (MQ=255)
                                                                                                                                                                      gATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTTATGCCGCCCTTAATAATCCCTTTC‑GTCGCCTGTTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTc                                                                      <  1:505557/251‑1 (MQ=255)
                                                                                                                                                                          ttGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACg                                                                   <  1:111203/250‑1 (MQ=255)
                                                                                                                                                                                    aaTGCCAACGTTAAAGACACCGTCAGTGCCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGATCCAGCCCGGCACCGCCATTGGGACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGCCACGATGCCAAAGTTATCCAGCTCACGAAGTTCTTTg                                                         >  2:589438/1‑250 (MQ=255)
                                                                                                                                                                                              ttAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATCGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAAttt                                              >  1:380861/1‑251 (MQ=255)
                                                                                                                                                                                                        cGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTAGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAgcc                                    >  2:539692/1‑249 (MQ=255)
                                                                                                                                                                                                              tGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTCC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGAt                               >  2:206569/1‑250 (MQ=255)
                                                                                                                                                                                                                      aaaaTGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCa                       >  1:442759/1‑250 (MQ=255)
                                                                                                                                                                                                                                     ccgccCTTCATAAACACTTTC‑GTCGCCTGCTGATAAGCCAGTTCCCGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAAt       >  2:342273/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gccCTTAATAAACCCTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATc      >  1:404779/1‑250 (MQ=255)
                                                                                                                                                                                                                                          cTTAATCAACACTTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCCGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGCAGACTGGATCAGTGGCAAACGTTCATCAGAACTCATTg  >  2:558116/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
ATTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTC‑GTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTG  >  NC_000913/2384562‑2385046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑