Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,209	A→G	W197R (TGG→CGG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,209	0	A	G	100.0%	223.9 / NA	67	W197R (TGG→CGG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/31);  total (36/31)

ACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGG  >  NC_000913/2328962‑2329455                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aCTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAACCCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGt                                                                                                                                                                                                                                                     >  1:264476/1‑251 (MQ=255)         cgtcctctTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCAcc                                                                                                                                                                                                                                              <  1:348406/246‑1 (MQ=255)         cgtACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCAccc                                                                                                                                                                                                                                             <  1:202414/251‑1 (MQ=255)            aCTCGTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGCACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTa                                                                                                                                                                                                                                          <  2:160249/251‑1 (MQ=255)                 tttCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCg                                                                                                                                                                                                                                      >  1:101996/1‑250 (MQ=255)                  ttCCGCTGTGATTTTCTTCAGCGGATAGCGGTCAAACAGCTCCCCCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTGCACCCGTACCCGa                                                                                                                                                                                                                                     <  2:78744/250‑1 (MQ=255)                      gCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTAcc                                                                                                                                                                                                                                <  2:138760/251‑1 (MQ=255)                       cTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCg                                                                                                                                                                                                                               >  1:163959/1‑251 (MQ=255)                                     aGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGac                                                                                                                                                                                                                 <  1:14115/251‑1 (MQ=255)                                     aGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTGCACCCGTACCCGACTACCGAGCATGGATGCGac                                                                                                                                                                                                                 <  2:400690/251‑1 (MQ=255)                                                  cAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGTCGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGtttt                                                                                                                                                                                                                                                  <  2:19103/205‑1 (MQ=255)                                                  cAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGtttt                                                                                                                                                                                                                                                  >  1:19103/1‑205 (MQ=255)                                                  cAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTGCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGcc                                                                                                                                                                                                    <  1:529585/251‑1 (MQ=255)                                                   aaaCAGCTCCCCCTCTACACATTGCCCACTGGCAACCTGCGTCTGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGTGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCt                                                                                                                                                                                                   <  2:94204/251‑1 (MQ=255)                                                    caccgttCCACCACTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCTTCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCAGCGGCAAACCAGCTCCGGGGTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTc                                                                                                                                                                                                  <  2:217028/245‑1 (MQ=255)                                                    aaCAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTc                                                                                                                                                                                                  <  2:166570/251‑1 (MQ=255)                                                              ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcacc                                                                                                                                                                                        >  2:384192/1‑251 (MQ=255)                                                                  tACACATTGCCCACTGACAACCTGAGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTAACATCGGCAAACAAGCTCCGGGTTTTAACCCGTACCCGACTACCGAGCATGGAAGCGCCACCGATTTTCGCCTCGTCCAGCACcacac                                                                                                                                                                                    >  2:332864/1‑250 (MQ=255)                                                                      caTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAg                                                                                                                                                                                <  1:554675/251‑1 (MQ=255)                                                                      caTTGCCCACTGGCAACCTACGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGAACCCGACTACCGAGCCTGGGTCCGACACCGCTTTTCGCCTCGTCCAGCACCACATcc                                                                                                                                                                                  >  2:339088/1‑249 (MQ=255)                                                                            ccACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCCTGGATGCGACACCGATTTTCGCCTCGTCCAGCACCCCATCCAGTAAGAc                                                                                                                                                                          >  2:471466/1‑251 (MQ=255)                                                                             cACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCACCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGCTGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACt                                                                                                                                                                         >  2:343396/1‑251 (MQ=255)                                                                              aCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATggct                                                                                                                                                                                                                      >  1:588951/1‑203 (MQ=255)                                                                              aCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGAt                                                                                                                                                                                                                      <  2:588951/205‑1 (MQ=255)                                                                                    aaCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTg                                                                                                                                                                    >  1:261433/1‑249 (MQ=255)                                                                                      ccTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCt                                                                                                                                                                <  1:318361/251‑1 (MQ=255)                                                                                      ccTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCt                                                                                                                                                                <  2:270107/251‑1 (MQ=255)                                                                                                          aCCCGATTAAGCACCGTCGCATAACGGGGTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGGCCATCACCACATCCAGTAGGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGt                                                                                                                                            <  2:101470/251‑1 (MQ=255)                                                                                                               aTTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCt                                                                                                                                        >  1:447543/1‑250 (MQ=255)                                                                                                                        cGTCGCATAACGGGTTAGTACCGGTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCGCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCaa                                                                                                                              <  1:293171/251‑1 (MQ=255)                                                                                                                           cGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGCGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTCACGTCGCTAATGAATCAagc                                                                                                                            >  2:579241/1‑250 (MQ=255)                                                                                                                                aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACGCCGATTTTc                                                                                                                                                                                                       <  2:50368/170‑1 (MQ=255)                                                                                                                                aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTc                                                                                                                                                                                                       >  1:50368/1‑170 (MQ=255)                                                                                                                                   gggTTAGTGCCGTTTTGATGGGGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTa                                                                                                                   <  2:3700/251‑1 (MQ=255)                                                                                                                                   gggTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTa                                                                                                                   <  2:163959/251‑1 (MQ=255)                                                                                                                                     gTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAAc                                                                                                                 <  1:427242/251‑1 (MQ=255)                                                                                                                                         gTACCGTTTTGCTGGTGCCAATGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGtt                                                                                                             >  2:39054/1‑251 (MQ=255)                                                                                                                                             cGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCg                                                                                                         >  1:183918/1‑251 (MQ=255)                                                                                                                                              gTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCg                                                                                                         >  2:448747/1‑250 (MQ=255)                                                                                                                                                tttGCTGGTGCCACTGCCCGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAACCCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGCCACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAgg                                                                                                      >  2:256709/1‑251 (MQ=255)                                                                                                                                                   gCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGttt                                                                                                   <  1:548850/251‑1 (MQ=255)                                                                                                                                                           cACTGCCAGTAACCCACAGCGGTGTACCAGCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGCCTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCCGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGACTCAAGCAGCGCTTTAACCGTTACCGCGGTTTCTGGCTTc                                                                                           >  2:480166/1‑251 (MQ=255)                                                                                                                                                            aCTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCTGCCAGCAAAACCGCCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTTCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCTTTATCGAGGTTTCTGGCTTCa                                                                                          <  2:405049/251‑1 (MQ=255)                                                                                                                                                                    tAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGAGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTc                                                                                  >  1:131417/1‑251 (MQ=255)                                                                                                                                                                     aaCCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTc                                                                                  >  2:18080/1‑250 (MQ=255)                                                                                                                                                                       cccACAGCGGTGAACCATCGGCTAACCCCCCCGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCAGGGTTTCCACCCGTACACGCATACCGCGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAAACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCagg                                                                               >  2:341803/1‑250 (MQ=255)                                                                                                                                                                             gcgttgtACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACACGTACCCGACTACCTAGCATGGATGCGACCCCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCt                                                                         <  1:17280/247‑1 (MQ=255)                                                                                                                                                                                    aCCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTACCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTCCCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCGGAGCTTTGTGTTGCGCGGGTAACTTCGGTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCGGCGTAGTCAGAGACGCTAATCAc                                                                   <  2:364030/250‑1 (MQ=255)                                                                                                                                                                                      cATCGGTTAACCAGCCAGCAAAACCTCCCTGACGGATTTCCTGTTTATCAGCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCa                                                                <  2:165759/251‑1 (MQ=255)                                                                                                                                                                                          ggTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTg                                                            >  1:498203/1‑251 (MQ=255)                                                                                                                                                                                           gTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTACACCCGAACCCGACTAACGAGCATGGATGCGACACCGATTTTCGACTCGTCCAGCACCACATCCAGTCAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTAGATTCCGCGTAGTCAGAGACGCTAACCACGAAggggc                                                           >  2:51584/1‑248 (MQ=255)                                                                                                                                                                                             tAACCAGCCAGCAAGACCACCCTGACGGATTTCCTGTGTATCATCGGCAAACCAGCTGCGGGTTTTCACCCGTGCCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTGGTTTTGCGCGGTTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGcc                                                          <  1:292633/250‑1 (MQ=255)                                                                                                                                                                                                 cagccagcAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGGAGTCAGAGACGCTAACCACGCAGGTGCCTGACg                                                     <  2:579270/251‑1 (MQ=255)                                                                                                                                                                                                  agccagcAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGc                                                    >  1:171212/1‑251 (MQ=255)                                                                                                                                                                                                     cagcAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGt                                                 >  1:323034/1‑251 (MQ=255)                                                                                                                                                                                                       gcAAAACCTCCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTg                                               <  1:357822/251‑1 (MQ=255)                                                                                                                                                                                                                 ccTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTAACGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTCACGTCGCTAATGACTCAAGCTGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCTGAGACGCTAACAACGCAGGTGCCTGACGATGTTGCCAGTATTGc                                     >  1:257457/1‑251 (MQ=255)                                                                                                                                                                                                                      cGGATTTCCTGTTTATCCTCGGCAAACCAGCTCCGGGATTTCACCCGTACCCGAATACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGccccc                                 >  2:523126/1‑250 (MQ=255)                                                                                                                                                                                                                       ggttttCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCcac                               <  1:321961/248‑1 (MQ=255)                                                                                                                                                                                                                                   tATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGcc                    >  1:245024/1‑250 (MQ=255)                                                                                                                                                                                                                                           gCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCa            >  1:188347/1‑250 (MQ=255)                                                                                                                                                                                                                                             aaaCCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGCTTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGTCGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAAcc         >  2:282774/1‑251 (MQ=255)                                                                                                                                                                                                                                               aCCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCACCCGc       >  2:107744/1‑251 (MQ=255)                                                                                                                                                                                                                                               aCCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGc       >  1:32526/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCCGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATCTGCAACCGCTg     >  2:44401/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gCTCCGGGGTTTCAACCGTACACGACTACCGAGCATGGATGCGACCCCGTTTTTCGCCTCGTCCAGCACCACATCCAGTACGACTTCCTGCGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCCATATGCAACCGCTgcg   >  2:268541/1‑251 (MQ=255)                                                                                                                                                                                                                                                    cTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACCCCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGTGCGGGTAACGTCGCTAATGAATCAAGCAGAGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTgcgg  >  1:61429/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGG  >  NC_000913/2328962‑2329455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑