Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,188,087	T→C	T110A (ACG→GCG)	yehA ←	putative fimbrial adhesin YehA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,188,087	0	T	C	100.0%	78.9 / NA	25	T110A (ACG→GCG)	yehA	putative fimbrial adhesin YehA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/9);  total (16/9)

CGGGTTGTATGACAATGTCAGATTAAAGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAACAACCA  >  NC_000913/2187842‑2188320                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          cGGGTTGTATGACAATGTCAGATTAAAGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTg                                                                                                                                                                                                                                      >  2:437573/1‑251 (MQ=255)         aTGACAATGTCAGATTAAAGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGACGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAAcc                                                                                                                                                                                                                               >  1:259162/1‑250 (MQ=255)                         aaaGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCCGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCt                                                                                                                                                                                                              >  1:113176/1‑251 (MQ=255)                                 aGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGGCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGattatt                                                                                                                                                                                                       <  1:564152/250‑1 (MQ=255)                                     acCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGAc                                                                                                                                                                                                   >  1:34524/1‑250 (MQ=255)                                         gCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACg                                                                                                                                                                                               <  2:34524/250‑1 (MQ=255)                                               tgtgCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCGCCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGTCGCCCCCCGCAGAAGCATTCACTTTTGCATTACTGGCCGCAGGTGTCACCTCAACTGAATAACCACTGGTGAAACTGGTCTGATTATGTGACAACGTGAtgt                                                                                                                                                                                         <  1:508597/250‑1 (MQ=255)                                                       tGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACATGATGTTGTTTTTAt                                                                                                                                                                                >  2:122202/1‑251 (MQ=255)                                                                        ttACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATATCCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATg                                                                                                                                                                <  2:329767/250‑1 (MQ=255)                                                                                             tGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTACTCATCACTGAACCGCCGCCCCCCGCCGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTTTTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCg                                                                                                                                          >  2:222620/1‑251 (MQ=255)                                                                                              gCCACCTAATAAACACAGTAGAAATTGGACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCTCAGAAACATTCACTTGTGCATGTCTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCg                                                                                                                                          <  1:263210/250‑1 (MQ=255)                                                                                                           cacaGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCcaac                                                                                                                            >  1:501295/1‑251 (MQ=255)                                                                                                                  gAAATTGGACTGCGACAATCCCGCGTATCGATGATGAAGCACGGGATAATGTCGCAACACAGTTAATCATCACTGAACCGCCGCCCCCAGCAGAAACATTCACTTTTGCATTACTGGCAGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAGCTGGCCTGGTTATTTGACAACGTGATGTTATTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                                      >  1:251445/1‑250 (MQ=255)                                                                                                                             gcgccACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTGATAAAGTCCAACAATAATATCCTACggg                                                                                                            <  2:489491/245‑1 (MQ=255)                                                                                                                                     cccGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGGGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGAAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAgggg                                                                                                  >  1:204698/1‑251 (MQ=255)                                                                                                                                     cccGCGTGCCGATGAGGAAGCACTGGATAATGTCACAACACCATTAATCATCACTTAACCGCCTCCCCCCGCAGAAACATTCACTGTTGCATTTCTTGACGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATTGTTATATATTGTAACAATAatattcttcggactcaaagggg                                                                                                  <  1:468850/251‑19 (MQ=255)                                                                                                                                                                           cacCATTAATCATCACTGCACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGATTCATTaaa                                                             >  2:175988/1‑250 (MQ=255)                                                                                                                                                                            acCATTAATCATCACTGACCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTAAGCATGACGAATCATTAAAAt                                                           >  1:97706/1‑251 (MQ=255)                                                                                                                                                                                  aaTCCTCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTTGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAAGTATGCt                                                     <  2:113176/251‑1 (MQ=255)                                                                                                                                                                                                                aaCATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCCACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTg                       >  2:82361/1‑251 (MQ=255)                                                                                                                                                                                                                              cATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTg          >  1:30243/1‑250 (MQ=255)                                                                                                                                                                                                                                 tACTGGCCGAAGGGATCACCACAACTGAATAACCCCTGGTGAAACTGGACTGATTATTTGACAACGTGATGTAGTTTTTATTGGGCCATTCAAAATGAAAATTTAATTTTATGGTATCGTTATAAAGTACAAGAATCATATCCGACGGGCTCATAGGGTTGATAGTATTTGTACTTACGCATGCCAAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTTCAAAATCCCCCTGaac       >  2:396747/1‑250 (MQ=255)                                                                                                                                                                                                                                     ggCCGCAGGTGTCACCGCAACTTAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCTTTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAACAAcc   <  2:494421/250‑1 (MQ=255)                                                                                                                                                                                                                                     ggCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACTACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAACAAcc   >  1:351459/1‑250 (MQ=255)                                                                                                                                                                                                                                       ccGCAGGTGTCCCCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTACAATATCCCCCTGAACAACCa  >  2:21753/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          CGGGTTGTATGACAATGTCAGATTAAAGGAATAGACACCTGCATTTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAACAACCA  >  NC_000913/2187842‑2188320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑