Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	98.0%	159.3 / NA	51	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/26);  minor base G (0/1);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAA‑GATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAA‑CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGC  >  NC_000913/2173199‑2173693                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      ccGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAA‑GATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAA‑CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGc                                                                                                                                                                                                                                                    >  1:98061/1‑251 (MQ=255)               cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAA‑GATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAA‑CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGt                                                                                                                                                                                                                                      >  1:268513/1‑251 (MQ=255)                ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAA‑GATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAA‑CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGtt                                                                                                                                                                                                                                     >  1:42467/1‑251 (MQ=255)                       aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAA‑GATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAA‑CAGCACTTACAATGGCGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAccat                                                                                                                                                                                                                              >  2:27499/1‑251 (MQ=255)                              cgagccacgcccaCCGCCATCGCCACGAAGAAGCCGATGGTGGCAA‑GACCGCCAAACGCCAGACCCAGATTCCCCGGCCCAAAAA‑CGGCAATCAAAATGGGGAGTGGGATAAAAACAAGGCTTGCCGATACCACC‑‑GCCGTATGGCCCCGCTGCAACGCCGGACTACGGCCATTCAGGAACTCCTGAACGCCGAACTTCGCAGGGAAAAGAAGAAGAGCTTGCTCAGCGATGGGCGTTAAACCATCCatgat                                                                                                                                                                                                                       <  1:543546/239‑1 (MQ=255)                                     cGGCCACCGCCATCGCCACGAAGATGCCGATGGTGGTAA‑GATCGCCAAACTGCATCCCCTGATTCCCCGCCACACAAA‑CATCAATTAAAATGGGGAGTGGGATAAAATTCAGGCTTGCCTATACCACC‑‑GCCGTAGGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAATCGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa                                                                                                                                                                                                                <  1:358132/251‑1 (MQ=255)                                       gcgccggCCACCGCCCCGAAGAAGCCGAGGGTGGCAA‑GACCGCCAAACGGCAGCACCTGATAACCGGGCACACAAA‑CAGCAATAAAAATGTGGAGTGGAAAAAAAATCGGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCAGCCATGATGGATTTAAt                                                                                                                                                                                                              <  2:105767/245‑1 (MQ=255)                                        cccccgccaccgccaCGAAGAAGCCG‑TGGTTGCAAAGAACGCAAACCGGCAGCACCGGATTACCCGG‑ACCCAAAACAGCAATAAAAAGGGGGAGGGGGATAAAAATCAGGTTGGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc                                                                                                                                                                                                             <  2:310936/248‑1 (MQ=255)                                                        gaagaaGCCGATGGTGGCAA‑GATCGCCAAACGGCAGCACCTGATTCACCGGTACACAAA‑CAGCAATTAAAATGGAGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTagc                                                                                                                                                                                              >  1:235790/1‑250 (MQ=255)                                                               ccGATGGTGGCAA‑GATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAA‑CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACAACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGAAGCATCAc                                                                                                                                                                                      >  2:281410/1‑251 (MQ=255)                                                                                cGCCAAGCGGCAGCACCTGATCACCCGGCACAAAAA‑CACCAATAAAAAGGGTGAGGGGGATAAAAACCAGGCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAcc                                                                                                                                                                      <  2:42467/251‑1 (MQ=255)                                                                                   cAAACGGCAGCACCTGATTCCCCGGCACACAAA‑CAGCAATTAAAAGGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                                                                                   <  1:139087/251‑1 (MQ=255)                                                                                          cagccCCTGATTACCCGGCACACAAA‑CACCAATTAAAATGGGGAGGGGGATAAAAACCAGGCTGGCCGATAC‑‑CGCCGCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCa                                                                                                                                                            <  1:518367/251‑1 (MQ=255)                                                                                                 tGATCACCCGGCACAAAAA‑CACAAATAAAAATGGGAAGGGGGATAAAAACCGGGCTTGCAGATAC‑‑CCCCACCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAATTCGCCTGTAAACGACTACGAGCTTGCGTAGCGATGGGCGTTAAACCATCCATGATGGGGTTAATCACCCGGGTCATTCGCAGCAGCACTGCCGCCGTTTTGACCGCCAGTTGCAATacac                                                                                                                                                      <  2:391132/250‑1 (MQ=255)                                                                                                   aTTACCCGGCACACAAA‑CAGCAATTAAAAGGGTGAGGGGGATAAAAACCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCtt                                                                                                                                                   <  1:144282/251‑1 (MQ=255)                                                                                                              cccgatctagaaaTTAAAAGGGTGAGGGGGATAAAAATCAGGCGTCCGGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGt                                                                                                                                          <  2:454948/239‑1 (MQ=255)                                                                                                                      aGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGt                                                                                                                                          >  1:454948/1‑242 (MQ=255)                                                                                                                              aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTGTGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAATCCTGAACGCCGAACTTCGCCTCTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATACCg                                                                                                                         >  1:555137/1‑251 (MQ=255)                                                                                                                                agggTGAGTGGAATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                                                                                                       <  1:236863/249‑1 (MQ=255)                                                                                                                                                    cAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCt                                                                                                                                                                                                                                                   >  1:341178/1‑107 (MQ=255)                                                                                                                                                    cAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAc                                                                                                   >  1:480034/1‑251 (MQ=255)                                                                                                                                                       gCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCa                                                                                                <  1:246513/251‑1 (MQ=255)                                                                                                                                                         ttGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACg                                                                                              >  2:187373/1‑251 (MQ=255)                                                                                                                                                              ccgatacccccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCATCCCCATCACAAAACCCACGGTg                                                                                           <  1:143640/244‑1 (MQ=255)                                                                                                                                                                 atcccaccgccgTATGTCCCAGCAGCAACGCCGGATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGAGGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACa                                                                                        <  1:69484/245‑1 (MQ=255)                                                                                                                                                                 ataccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGCGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTgcc                                                                                         >  2:234705/7‑248 (MQ=255)                                                                                                                                                                     caccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATAACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCt                                                                                    >  2:176393/3‑251 (MQ=255)                                                                                                                                                                      cccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTc                                                                                   <  1:467157/251‑1 (MQ=255)                                                                                                                                                                                    cagcagCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACAATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGAGAGGATGCAGAAAATCAGCCCCATCACAAAATCCACGGTGACAGGCTCGCCAAATGGAccc                                                                      >  1:538085/1‑249 (MQ=255)                                                                                                                                                                                     agcagcAACGCCGGACCAAGGCAAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGGGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCaaa                                                                    <  1:297428/251‑1 (MQ=255)                                                                                                                                                                                     agcagcAACGCCGAATCAAGGCCAACCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCaaa                                                                    <  1:292677/251‑1 (MQ=255)                                                                                                                                                                                               ccGGATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGAGGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGa                                                          <  1:56113/251‑1 (MQ=255)                                                                                                                                                                                                  gATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAat                                                        >  1:19448/1‑250 (MQ=255)                                                                                                                                                                                                     cAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcg                                                    >  2:57123/1‑251 (MQ=255)                                                                                                                                                                                                             aTCAGGAACCCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTTCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCt                                            <  1:390457/251‑1 (MQ=255)                                                                                                                                                                                                                aggaaccacTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCGTGCTTAGCGATGGGCGTTAAACCATCCATGATGGTTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAAGGGACCAAATTTGCGCTGAATATCGTCGGCGCTaaa                                         <  2:426232/243‑1 (MQ=255)                                                                                                                                                                                                                    ccTCCCGACCGCAGAGCTTCCCCTGTGAACGACTGCGATCTTGCGTCGCGATGGGCGTTAAACCATCCATGATGTTTTTAATCACCAGTGGCATTAGCTGCATCACTTCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCTCGAGGATGCCGAGAATCAGCCCAATCACAAAACCCACGGTGACAGGTGCGTCAAATGGACCATAGTTGTGCTTAATATCGTCTGCGCTAAATTTa                                     <  2:60520/250‑1 (MQ=255)                                                                                                                                                                                                                       ccTGACCGCCGGACTTCGCCTGTAAACGACGACGAGCTGGCTTAGTGATGGGCGTTAAACCCGCCATGATGGGTTTAATCACCCGTGGCAGTAGCAGCGGCACTGCCGCCGTTTTTACCTCCAGCTGCAATACACCTTTGACATCGTAACCCGCGGGGATGCGGATAATCAGCCCCATCACAAAACTCAGGGTTACATGCTGGCCAAATGGACGAAATTGGGGTTTTATAGCGACGGCGCTAAATTTaa                                    <  2:235790/249‑1 (MQ=255)                                                                                                                                                                                                                       ccTGACCGCCGACCTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAAtt                                  <  2:98061/251‑1 (MQ=255)                                                                                                                                                                                                                       ccTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGAACCAAATTTGCGCTGCATATCGTCGGCGCTAAATTTAAtt                                  >  1:186115/1‑251 (MQ=255)                                                                                                                                                                                                                          gACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTc                                 <  1:519230/249‑1 (MQ=255)                                                                                                                                                                                                                                     ttCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCCATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTgg                    >  1:69857/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccTGTAAACGTCGACGGGCTTCCTGAGCGATGTGCGTTAAACCATCCATGATGGGTTTAATCCCCCTTGCCATTAGCAGCATCACTGCCTCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCCCGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATCTCGTCTGCGCTAAATTTAATTCGGTTACCGCCTGGGAtt                <  2:480034/251‑1 (MQ=255)                                                                                                                                                                                                                                            gTAAACGACTACGGGCTTGCTTAGCGATGGGCGGTAAACCATCCATGAAGGATTTACTCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAAAACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCAACGGTGACAGGCTCGCCAAAAGGAACAAATTTGCGCTGAATAACGACGACGCTACATTTAATTCGATTAACGCCTGGAAtttac             >  2:34403/1‑249 (MQ=255)                                                                                                                                                                                                                                                  gACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcgtcg                                                 >  1:245186/1‑209 (MQ=255)                                                                                                                                                                                                                                                  gACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcgtcg                                                 <  2:245186/209‑1 (MQ=255)                                                                                                                                                                                                                                                   aCTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCgatg       >  1:164251/1‑250 (MQ=255)                                                                                                                                                                                                                                                   aCTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCgatg       >  1:156116/1‑250 (MQ=255)                                                                                                                                                                                                                                                      aCGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCgttgat     >  2:274973/1‑249 (MQ=255)                                                                                                                                                                                                                                                      aCGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTGCTCGATGATAg   <  1:144572/251‑1 (MQ=255)                                                                                                                                                                                                                                                        gAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGc  >  2:568964/1‑250 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      CCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAA‑GATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAA‑CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGC  >  NC_000913/2173199‑2173693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑