Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,137,543 G→A intergenic (‑300/‑359) wza ← / → yegH outer membrane polysaccharide export protein Wza/inner membrane protein YegH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,137,5430GA94.1% 44.6 / NA 17intergenic (‑300/‑359)wza/yegHouter membrane polysaccharide export protein Wza/inner membrane protein YegH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (8/8);  minor base C (1/0);  total (9/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

GTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAGGGATAAT  >  NC_000913/2137297‑2137780
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             
gTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTg                                                                                                                                                                                                                                            >  2:425245/1‑250 (MQ=255)
                                      aaTTTAACTGGTATCAGCCCTAAAAAAACTGATGTCATCCGCAGTCTATGTACAGAATAATTCAGACTGTTCTTTAAGGCATCCAGACCCGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATTCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCag                                                                                                                                                                                                     <  1:139919/251‑1 (MQ=255)
                                              tGGCATCCGTCCTAAACTAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCCAACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGCTTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCATTAATGCAGGAGGAAgg                                                                                                                                                                                             >  2:189454/1‑250 (MQ=255)
                                                     aGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATCCAGCCTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACGGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTGCCGAATATAAAGTGACATTATGGTAATTGGATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAg                                                                                                                                                                                      <  1:54095/251‑1 (MQ=255)
                                                                aaTTGATTTCACCCGCAGGCTATTGACAGAATAATTCATACTGGTCTTTCAGGCATCCAGACACGCTTCCGCCCCTGGCTTTTTAGCTACCAATACACTGAGTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAAATGAATATTGGCTTTCCAATAATGCAGGATGAAGTGTTACAGCTAACGGAATa                                                                                                                                                                           <  2:368434/251‑1 (MQ=255)
                                                                       ttCATCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTACTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATCATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGc                                                                                                                                                                     >  2:283574/1‑250 (MQ=255)
                                                                                      ttGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGt                                                                                                                                                     >  1:418493/1‑251 (MQ=255)
                                                                                              ataaaTCAGACTGGGCTTTCAGGCAGCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTCCCAAGAATTCAGGAGGAATTGTTACAGCTAACGGAATAGCACGCAAGATAACAATGTGGTAATTGGCt                                                                                                                                             <  2:382720/251‑1 (MQ=255)
                                                                                                        cTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTa                                                                                                                                      >  1:542695/1‑248 (MQ=255)
                                                                                                                                            ggCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAg                                                                                               <  1:523573/251‑1 (MQ=255)
                                                                                                                                                     gCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCttt                                                                                        >  2:309863/1‑249 (MQ=255)
                                                                                                                                                                                                  aTGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAACGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAgt                                                                                                                       >  1:368642/1‑173 (MQ=255)
                                                                                                                                                                                                  aTGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAgt                                                                                                                       <  2:368642/173‑1 (MQ=255)
                                                                                                                                                                                                                     aGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTAcc                      <  2:418493/251‑1 (MQ=255)
                                                                                                                                                                                                                                ggAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTc           >  1:277666/1‑251 (MQ=255)
                                                                                                                                                                                                                                     ttAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAggg       >  1:547988/1‑250 (MQ=255)
                                                                                                                                                                                                                                           tCCGAATATAAAGTGACATTATTGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAGGGATAAt  <  2:14810/249‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             
GTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAGGGATAAT  >  NC_000913/2137297‑2137780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑