Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,134,498	G→A	V385V (GTC→GTT)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,134,498	0	G	A	100.0%	174.9 / NA	53	V385V (GTC→GTT)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/25);  total (28/25)

AGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCC  >  NC_000913/2134254‑2134741                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACctgc                                                                                                                                                                                                                                               >  2:289817/1‑251 (MQ=255)   aCGCTCATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGAGATTAATCAACGAGAGCAGCAGCACACCCACGATAGAACGCATCAGGCCAAGGATAATCGCGCCGCGGATACTCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACACTGCGCACATCGCCGACGGTGCTGGCCTAGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATCTAAACctgctg                                                                                                                                                                                                                                             >  2:485263/1‑251 (MQ=255)    cGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACctgctg                                                                                                                                                                                                                                             >  1:68737/1‑250 (MQ=255)        aaTACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGAcc                                                                                                                                                                                                                                          <  1:289817/249‑1 (MQ=255)            ccGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTc                                                                                                                                                                                                                                    >  1:301220/1‑251 (MQ=255)                                 gggCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGCCGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGAc                                                                                                                                                                                                               >  2:276679/1‑251 (MQ=255)                                                cgATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCTGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGt                                                                                                                                                                                                <  1:462458/251‑1 (MQ=255)                                                 gATTAACCAACGAGCGCAGCCGCACACCCACGATAGCCAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGCTTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCCTATAAACCTGCTGCCCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGtt                                                                                                                                                                                               >  2:112052/1‑251 (MQ=255)                                                 gATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGtt                                                                                                                                                                                               >  1:484235/1‑251 (MQ=255)                                                    tAAACAACGATCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACAGCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCGGCTCTTTATTCAGCAGTGGCAGATAAACCTGCTGTCCAGACTCGACATCGCGGGTCACACGGACAATCTCCGGCTAGGtttt                                                                                                                                                                                             <  2:54531/250‑1 (MQ=255)                                                     aaaCAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCg                                                                                                                                                                                           <  1:322329/251‑1 (MQ=255)                                                           cGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCg                                                                                                                                                                                     >  2:501665/1‑251 (MQ=255)                                                                   gcagcaCACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTTAGACGGACAATCGCCTGCTGGGGTTTCGGCATCGCCGTTAcg                                                                                                                                                                             <  1:161463/251‑1 (MQ=255)                                                                       cacccccACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTGCGGTTGCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCGTTATTCAGCAGTTGCAGATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACCGACAATCTCCTGCTGTGTTTTCGGCATCGCCGTTAcgcgtc                                                                                                                                                                         <  2:567888/247‑3 (MQ=255)                                                                               cGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGt                                                                                                                                                                 <  1:3747/251‑1 (MQ=255)                                                                                 aTAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGttt                                                                                                                                                               <  1:11203/251‑1 (MQ=255)                                                                                 aTAGAAAGCATCAGGCAAAGGCTAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGttt                                                                                                                                                               >  2:447007/1‑251 (MQ=255)                                                                                   aGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGTGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTgg                                                                                                                                                             >  1:37390/1‑251 (MQ=255)                                                                                   aGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGGCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTgg                                                                                                                                                             <  2:483894/251‑1 (MQ=255)                                                                                     aacgCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGTGTAAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATGCAGCGGTTGCATATAAACCTGCTGACCAGACTCGACATCTCGTGTCATACAGACAATCTTCTGCTGGGTTTTCGGCATCTCCGTTACGCGCCCGTATAGTTTgtct                                                                                                                                                           >  2:160748/4‑248 (MQ=255)                                                                                                   aGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAAGCTGAGTGATTGCCAGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGATCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGAACAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGATGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTcc                                                                                                                                               >  1:366986/1‑249 (MQ=255)                                                                                                         aTCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTg                                                                                                                                       <  1:495062/251‑1 (MQ=255)                                                                                                              gcCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGtt                                                                                                                                  <  1:226155/251‑1 (MQ=255)                                                                                                                cGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCctg                                                                                                                                                                                                      >  2:437675/1‑181 (MQ=255)                                                                                                                cGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCctg                                                                                                                                                                                                      <  1:437675/181‑1 (MQ=255)                                                                                                                 gAGGCTAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTGCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTtct                                                                                                                               <  2:179644/251‑1 (MQ=255)                                                                                                                  aggctAATCAGCCCTTTCTTCGGTTTCAGCCCGCCATGCTGAGTGAGTGCCGGGTCAACAATGCGCACATCGCCGACTGTGCTGGCCTCGGTGATGTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAACCCTGCTGACCAGCCTCGACATCGCGGGTCAGACGGACAAGCTCCTGCTGGGTTTTCGGCAGCGCCGTTACGCGACCGTGAAGTTTGGCTTTTGCTGCTTCCAGAGCCTGTCGTTtctc                                                                                                                              <  2:386907/247‑1 (MQ=255)                                                                                                                    gATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTcc                                                                                                                             >  1:195835/1‑250 (MQ=255)                                                                                                                           aGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGt                                                                                                                     <  1:341698/251‑1 (MQ=255)                                                                                                                                tttCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGt                                                                                                                >  1:273423/1‑251 (MQ=255)                                                                                                                                  tcttcGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAACCCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCCGCGTGCGGTAc                                                                                                              >  1:552603/1‑251 (MQ=255)                                                                                                                                     tcattttCAGCACGCCAGGCTGAGTGATTTCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTTATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGGCAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTAc                                                                                                              <  1:522757/244‑1 (MQ=255)                                                                                                                                                         tGAGTTATTGCCGGGTCAACAATGCTCACATCTCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGGTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACt                                                                                                  <  1:542749/240‑1 (MQ=255)                                                                                                                                                         tGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGCTTTTCAGCTCCTGCTCTTTATTCCGCAGTTGCATATAAACCTGCTGACCAGACTAGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTCAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGGACt                                                                                                  >  2:542749/1‑240 (MQ=255)                                                                                                                                                            gTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCGCGGTGATTTTCAGCTCCTGCGCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCTAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCtt                                                                                    <  2:196569/251‑1 (MQ=255)                                                                                                                                                                  gCCGGGTCAACAATGCGCACATCGCCGACCGTGCTGGCCTCGGTGATTTTCAGATCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGCCTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCTTGCGGTACGCCGGGCGAACTTTGGAGTACAACTTgg                                                                                  >  2:532403/1‑247 (MQ=255)                                                                                                                                                                    cGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGAttt                                                                            <  1:501081/251‑1 (MQ=255)                                                                                                                                                                      ggTCAACAATGCGCACATCGCCTACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTTCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTGCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTcc                                                                          <  1:369697/251‑1 (MQ=255)                                                                                                                                                                            caaTGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCGGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCctct                                                                    <  1:189305/251‑1 (MQ=255)                                                                                                                                                                             aaTGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGCCGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCtt                                                                   >  2:510696/1‑251 (MQ=255)                                                                                                                                                                             aaTGCGCACATCGCCGACGGTGCTGGCCTCGGTGAGTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCtt                                                                   <  2:484235/251‑1 (MQ=255)                                                                                                                                                                                        cgccgacgtggctggcggtggggATTTACAGCTCCTGCTCTTTAGTCAGCAGTTGCATATAAACCTGCTGACTAGACTCGACATCGCGGGTCAGACGGACAATCTGCTGCTGTGTTTTCGGCATCGCCGTTACGCGACCGTTAAGGTTGGCTGTTTCGTCGGCCAGCGCCTGTCTTTTCTCGAGCAGCGTGCGGGTCGCCGGGTGAACGTGGGTGTACAGCTGGGAGATGTCCGCCCCTTTAGATGTCAg                                                         <  2:344540/228‑1 (MQ=255)                                                                                                                                                                                          ccGACGTTGCCGGACTAGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGATGCATATAAACATGCTGACCAGACTCGTCATCGCGGGTCAGACGGCCAATCTCCTGCTGGGATTTCGGCATCGCCGATACGCGACCGTTAAGATTGGCTTTTTCGTCTTCCGGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGCACTTTGGTGGACAGCTTGGAGATTTCCGCCCCTTTAAAGGTCAgttc                                                      >  2:213837/1‑251 (MQ=255)                                                                                                                                                                                           cGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcg                                                     >  2:188369/1‑251 (MQ=255)                                                                                                                                                                                           cGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcg                                                     >  1:421682/1‑251 (MQ=255)                                                                                                                                                                                                gTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTGTAAAGGTCAGTTCGTTCaa                                                <  2:421682/251‑1 (MQ=255)                                                                                                                                                                                                     ggCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTgc                                            <  2:191686/250‑1 (MQ=255)                                                                                                                                                                                                     ggCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTgc                                            >  1:191686/1‑250 (MQ=255)                                                                                                                                                                                                                       cTCCTGCTATTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGTTGTTCACCatcg                         >  2:561685/1‑251 (MQ=255)                                                                                                                                                                                                                              tctTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCGGTTCGTTCAACTGCGCATCGATGTTCACCatcgaatcg                    >  2:93282/1‑249 (MQ=255)                                                                                                                                                                                                                                  tATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTCCTGGGTTTTCGACATCGCAGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGCTGTTCACCATCGAATCGAGCAcc              >  1:530783/1‑251 (MQ=255)                                                                                                                                                                                                                                            ttGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCtt    >  2:193866/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCATATAAACATGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATAGATGTTCACCATCGAATCGAGAACCGCTTTTGCTTcc  >  1:423110/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    AGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCC  >  NC_000913/2134254‑2134741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑