Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,086,634	G→A	A143A (GCC→GCT)	plaP ←	putrescine:H(+) symporter PlaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,086,634	0	G	A	100.0%	174.0 / NA	52	A143A (GCC→GCT)	plaP	putrescine:H(+) symporter PlaP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/27);  total (25/27)

TCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAACAGG  >  NC_000913/2086389‑2086877                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    tcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCgg                                                                                                                                                                                                                                                 <  1:447737/250‑1 (MQ=255) tcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCg                                                                                                                                                                                                                                                  <  2:49517/249‑1 (MQ=255)   cGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCa                                                                                                                                                                                                                                              <  2:273014/251‑1 (MQ=255)     aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGCCGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTCCGCAGGTTAAAAGCGGTCAt                                                                                                                                                                                                                                             >  1:406227/1‑250 (MQ=255)                       cAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAAGCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGGGCTCGCCAGCGTACCAGCGCCTTCACCATCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGccacc                                                                                                                                                                                                                          <  2:462408/251‑1 (MQ=255)                        aaaGCCGGTAAAGGAGAAGCACAGGATGGTCGCACCGGGAATCATCGGGATAACATGCGCATCGCCAGAACAGAATGGACGAGGGCTCGCCAGCGTACCAGCGCCTACACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCGGCAACACGACGATGACAGTGTTGAAGTTCGCTACGGATTTCAGACGACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGccacca                                                                                                                                                                                                                         <  2:256695/251‑1 (MQ=255)                              ggTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAAc                                                                                                                                                                                                                    >  2:510059/1‑250 (MQ=255)                               gTAAAGGAGAAGCAAAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGCAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACAt                                                                                                                                                                                                                  >  1:147064/1‑251 (MQ=255)                                  aaGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTTGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATcc                                                                                                                                                                                                                >  1:327876/1‑250 (MQ=255)                                    ggAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACg                                                                                                                                                                                                             <  1:142164/251‑1 (MQ=255)                                               ggATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGaa                                                                                                                                                                                                  <  1:81661/251‑1 (MQ=255)                                                       gCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCag                                                                                                                                                                                          <  1:70518/251‑1 (MQ=255)                                                       gCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCag                                                                                                                                                                                          <  2:451383/251‑1 (MQ=255)                                                               aaTCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATCAACAAAAATGCCCAGAACGACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGATACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCa                                                                                                                                                                                   >  1:564246/1‑250 (MQ=255)                                                               aaTCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGGTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCa                                                                                                                                                                                   <  1:116839/250‑1 (MQ=255)                                                                atcatcGGGATAACATGCGCACCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCaaa                                                                                                                                                                                 <  2:305142/251‑1 (MQ=255)                                                                        gATAACATGCGCGTCGCCAGACCAGAATGGCCAAGTGCTAGACAGCGTCACAGCGCCTTCACCTTCAAATACGCCATAACCAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACAAGACGATTACAGTGTAGAAGTTCGCTCCGGATTTCAGACTACGCAGGTTAAAAGAGGGCATAAAGGCAACCACCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAACAAGAttt                                                                                                                                                                         >  2:30708/1‑251 (MQ=255)                                                                             cATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCCTAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGcc                                                                                                                                                                    >  2:248983/1‑251 (MQ=255)                                                                                           gaccagaATGGACGAGTGAGCGCCCGCGTACCAGCGCTTTCACCTTCAAATACGCCGTAAACAACCATGCCCAGAAACTCCGCGATCAGCACTACCTGCAACACGACTATTACAGTGTTGAAGGTCGCTACGGATTTCAGACTACTCAGGTTAAAAGCGGTAATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCCGTAGAATGTTGat                                                                                                                                                      <  2:7124/251‑1 (MQ=255)                                                                                                    ggACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcg                                                                                                                                              <  1:458173/250‑1 (MQ=255)                                                                                                            gCTCGCCAGCGTACCAGCGCCTTCCCCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTTCGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGAt                                                                                                                                     <  1:228588/251‑1 (MQ=255)                                                                                                            gCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGAt                                                                                                                                     <  1:510059/251‑1 (MQ=255)                                                                                                              tCGCCAGCGGACCAGCGACTTCAGCTTAATGTACGACATAACCAACCCTGACCAGAATCACCGAGATCAGCACTACCTGCAACACGACGCCTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGCTTAAAAGCGGTCCTAAAGGCCACGAGCGCCACCACACACATCAACGCTGGGCTGGCAGGGACCAGAGCTTCCAAATAGATTTTCGACAGTCGACTGTTGATCATCGGcgcgcaccgat                                                                                                                                     >  2:235406/1‑242 (MQ=255)                                                                                                                   aGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCACAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCg                                                                                                                                >  1:28293/1‑249 (MQ=255)                                                                                                                      gTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGCATCACCGCGATCAGCACTACCTGCAACACGAAGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGAGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCCGAGATTAAAAATAGAATTTCGCCCGTGAAATGTTGATCATAGGAGCGGACAgcacgtcgcgc                                                                                                                             >  2:252726/1‑239 (MQ=255)                                                                                                                        aCCAGCGCCTTCACCGTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGAGCAGCACTACCTGCAACACGACGAGTACAGGGTTGAAGTGCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCTCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATGTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCaga                                                                                                                          <  1:235406/250‑1 (MQ=255)                                                                                                                              gcCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGAcc                                                                                                                   <  1:15783/251‑1 (MQ=255)                                                                                                                                cttcaccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGACATAAAGGCCACCAGCGACACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAAACCa                                                                                                                  >  1:135703/1‑250 (MQ=255)                                                                                                                                  tcaccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGCAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCa                                                                                                                                                                                   >  2:289648/1‑183 (MQ=255)                                                                                                                                  tcaccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCa                                                                                                                                                                                   <  1:289648/183‑1 (MQ=255)                                                                                                                                     ccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCACAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACAAACccac                                                                                                            >  1:104908/1‑251 (MQ=255)                                                                                                                                     ccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccac                                                                                                            <  1:255337/251‑1 (MQ=255)                                                                                                                                      cttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccacc                                                                                                           >  2:505245/1‑251 (MQ=255)                                                                                                                                                                           cgcgATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTGCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGt                                                                      <  1:280779/251‑1 (MQ=255)                                                                                                                                                                             cgATCAGCACTAACTGCAACACGACGATTACAGTGTTGAAGTTAGCTACGGATTTCCGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCAACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAAACCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTa                                                                     >  2:353867/1‑250 (MQ=255)                                                                                                                                                                               aTCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTacgg                                                                  >  2:25985/1‑248 (MQ=255)                                                                                                                                                                                      cTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCAACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATg                                                             >  1:90840/1‑249 (MQ=255)                                                                                                                                                                                      cTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCAACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTGCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATg                                                             <  2:90840/249‑1 (MQ=255)                                                                                                                                                                                            gCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGcc                                                      >  1:576599/1‑250 (MQ=255)                                                                                                                                                                                              aaCACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCcagc                                                   >  2:222413/1‑251 (MQ=255)                                                                                                                                                                                                   gacgaTTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGa                                                >  1:154265/1‑249 (MQ=255)                                                                                                                                                                                                          aCAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGACGGATAgcg                                       <  2:354895/251‑1 (MQ=255)                                                                                                                                                                                                                ttGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGAAGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGACGACCAACCAACCATAAAGCCGAAAGTCGGGCTAATGGATTTCTGGGCGTCAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAAc                                 >  1:496848/1‑251 (MQ=255)                                                                                                                                                                                                                  gAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAATCATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCa                               <  1:490750/251‑1 (MQ=255)                                                                                                                                                                                                                            aCGGATTTCAGCCTACGCAGGTTAAAAGCGGTCATAAAGTCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAACAGATTTTCGCCAGTATAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGTCGTACGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGATCCAGCTTCCCg                       <  1:72693/249‑1 (MQ=255)                                                                                                                                                                                                                            aCGGATTTCAGACTCCGACGGTTAAAAGCGGTCATAAAGGCCTCCAGCGCCACCACAAAAATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTa                     <  2:135703/251‑1 (MQ=255)                                                                                                                                                                                                                             cGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGt                      >  1:181688/1‑249 (MQ=255)                                                                                                                                                                                                                             cGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAg                    <  1:490889/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTaa       <  1:518447/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAAc     >  2:110896/1‑251 (MQ=255)                                                                                                                                                                                                                                             cAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTATGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAAc     >  1:391108/1‑250 (MQ=255)                                                                                                                                                                                                                                                gTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGCGAATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAACAgg  >  1:378803/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    TCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAACAGG  >  NC_000913/2086389‑2086877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑