Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,083,929	C→T	P392S (CCG→TCG)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,083,929	0	C	T	96.9%	81.3 / NA	32	P392S (CCG→TCG)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/19);  minor base G (0/1);  total (12/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGA  >  NC_000913/2083697‑2084170                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  actaagaccGGAAGATGCCGAACGACTGGGTATTAAGCGCCAGCATTTCCTCGATAACCTTGAAACTCTTTGTGAGAACCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCGGAAGCGTGGTCGCCTGCAGATACCGTGGATGCTCAGCTTTATAACGGGTGTTTCAGCGACGAAGATCGTGCAGCAATGAAAATGGTGATTGAATCCGAGCCGCGTAAGTTAGCTGCCCTGGATATCACtt                                                                                                                                                                                                                                 <  2:400932/244‑1 (MQ=255)            aaGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGCAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTTGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATaa                                                                                                                                                                                                                       >  2:297642/1‑250 (MQ=255)                 gCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCATATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGa                                                                                                                                                                                                                  <  1:352147/250‑1 (MQ=255)                        gacTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAg                                                                                                                                                                                                          <  1:220832/251‑1 (MQ=255)                        gacTGGGAATTAAGCGTCAGCATAGCCTCGAGATCCGTAAAAGGCTGCGTGAAACTCCGCAATTGCGCGAAAAATTGGTTGCGATATTCGCGGAAGCCGATTCGTTTACGTCTGCAGATACCGTGGATGTACAGCGTTATTACTGCTTGTTCAGTGACGCAGATCGTGCAGCATTGAAAATTGTGCTGGAATCCGAGCCGCGTAATTTATCGGCATTGGATATCACTTTTGTTGATAAACGGATTGAAAAg                                                                                                                                                                                                          <  1:336959/251‑1 (MQ=255)                               aaTTAATCGTCAGCATTGCCTCGATAACCTGAAACTTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGCAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCtgttgt                                                                                                                                                                                                   >  1:149263/1‑251 (MQ=255)                                   aaTCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCaa                                                                                                                                                                                               <  1:560346/251‑1 (MQ=255)                                                    cGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACt                                                                                                                                                                              >  2:174794/1‑251 (MQ=255)                                                                 aTTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCt                                                                                                                                                                 >  1:321185/1‑251 (MQ=255)                                                                                ccGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCCAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGagc                                                                                                                                                   >  1:563359/1‑250 (MQ=255)                                                                                   cAAGTGCGCGAAAAAGGGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACTCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAAGTATCGGGCTCGCAACGTCCCGGGTACGCTGGATTATGCCGAtcagca                                                                                                                                               <  2:392374/251‑1 (MQ=255)                                                                                     aGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAAc                                                                                                                                             <  1:546740/251‑1 (MQ=255)                                                                                              aaaaGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACGTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGctggctgg                                                                                                                                    <  2:2309/251‑1 (MQ=255)                                                                                                           atatTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCa                                                                                                                       <  2:249554/251‑1 (MQ=255)                                                                                                             atTCGCGGAAGCCGAACCGTTTACTCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAAGTATCGGGCACGCAACTTCCCTGTGACGCTGGATTATGCCGAGCAGCAACGCGGGCTGGAGCACCGTCGCCAg                                                                                                                      <  2:295045/250‑1 (MQ=255)                                                                                                                    ggaGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAAGTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCTCTGGATATCACTTTTGTTGATATACGGATGGAATAGCTGTTGTTCAAGTATCGGGTACGAAACTGCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGATCCCCGTCGCCAGGTTTTCAc                                                                                                              <  2:405756/249‑1 (MQ=255)                                                                                                                    gAAGCCGAACCGTTTACGCCTTCAGATCACGTGGATGCACAGCTTTCTAACGGCTTTTTCAGTGACGAAGATCGAGCAGCAAAGAAAATTATGCTGGAAACCGAGCCGCGTAATTTATCGGCCCTGGATATCACTTTTGTTGATTAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGACTATGCCGAGCAGCAACGCTGGCTGGAGCACGGTCGCCAGTTCATCAc                                                                                                              >  2:505430/1‑251 (MQ=255)                                                                                                                    gAAGCCGAACCGTTGACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGTCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCAc                                                                                                              <  2:439023/251‑1 (MQ=255)                                                                                                                                  tACGCCTTCAGATAACGTGGATGCACAGCGTTATAACGGCTTTTTCAGTGAAGCAGAGCGTGCAGAAATGAAAATTGGGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCGCTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATAATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGATGGAGCACCGTCGCCAGGTCTTCACGCCAGAGtgcttg                                                                                                 <  2:18438/250‑6 (MQ=255)                                                                                                                                                aCGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCGCTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTGTTGCAGGGTTATGCtgat                                                                                  <  2:149263/251‑1 (MQ=255)                                                                                                                                                   gggaggCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGaa                                                                               <  2:512162/246‑1 (MQ=255)                                                                                                                                                       tGCACAGCTATACAACGGCTTTTTCAGGGACGACGAACGTACAGCACTGAAAATTGTGATGGCAACCGAGCCGAGTAACTTATCGGCACTGGATATCACTTTTGTAGATAAACGGATTGAAAAGCTGTTGTTCAATTCTCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCCACGCTCACTGGAGCACCGCCGCCAGGTCTTCAAGCCAGAGTTATTGCAGGGATATGCtgaggtattgg                                                                           >  2:278006/1‑243 (MQ=255)                                                                                                                                                                 tataACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGt                                                                 >  1:162624/1‑251 (MQ=255)                                                                                                                                                                                     cGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGTTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAATATGCCGATGACa                                             <  1:318485/251‑1 (MQ=255)                                                                                                                                                                                           tCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCtgatga                                                                                >  2:286370/1‑210 (MQ=255)                                                                                                                                                                                           tCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCtgatga                                                                                <  1:286370/210‑1 (MQ=255)                                                                                                                                                                                             gTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGaa                                      >  1:379725/1‑250 (MQ=255)                                                                                                                                                                                                       gAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTAGCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGt                           <  1:505719/251‑1 (MQ=255)                                                                                                                                                                                                         aaaTTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGGACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTa                         <  2:471452/251‑1 (MQ=255)                                                                                                                                                                                                                     aaaCCGAGCCGCGGAATGGATCGGCACTGGAGATCACTTTTTTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCGTGATTATGCCGTGCAGCAACGCTGGTTGGAGCACCGTCGGCAGGTCTGCACGCCAGAGTTTTTGCAGGGTTATGCGGATGAATTGCAGATGCTGGTACAACAATATGTCGAGGACAAAGAGATAGTGGTGCTGTTAAAAGCACTTTgg             <  2:566074/251‑1 (MQ=255)                                                                                                                                                                                                                       aCCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGc            >  2:542603/1‑250 (MQ=255)                                                                                                                                                                                                                                  tAATTTATCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGAAGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTAagcgta  >  2:16168/1‑247 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  GCTACGCCCGGAAGATGCCGACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGA  >  NC_000913/2083697‑2084170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑