Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,082,863	C→T	T36T (ACC→ACT)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,082,863	0	C	T	100.0%	117.9 / NA	35	T36T (ACC→ACT)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)

AGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT  >  NC_000913/2082616‑2083106                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    aGTGCAAGAAAATGTGACTACCCGCTCATGTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATGGTGGCGCTTAAGCTGATTAGCACTGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGGTTCACGATTACGAATCCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGAt                                                                                                                                                                                                                                                  <  2:214099/251‑1 (MQ=255)    gCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGc                                                                                                                                                                                                                                               <  2:487135/251‑1 (MQ=255)         aaaaTGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATATCGAAtt                                                                                                                                                                                                                                          >  1:406168/1‑251 (MQ=255)                  cTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCAt                                                                                                                                                                                                                                 >  2:295420/1‑251 (MQ=255)                    aCCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATc                                                                                                                                                                                                                                <  1:505502/250‑1 (MQ=255)                                                           cTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATAATCTGGCAGACAGAAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAACAACAATCTACCTTTTTGTTTCACGATTACGCAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACt                                                                                                                                                                                        >  2:214449/1‑251 (MQ=255)                                                             gCCAAATTGTGGCGCTAAAGCTGAGTAGCACGGTGATATTTGATACTCTGGCAGACAGCATAAATAACGGAGTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTGTTGTTTCACGATTCCGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCGGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACt                                                                                                                                                                                        <  2:322775/249‑1 (MQ=255)                                                                aaaTTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTGGTACTGCAAGCCCGCTTATGACTATTTa                                                                                                                                                                                   <  2:298680/251‑1 (MQ=255)                                                                            tAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAACTAACGGATTTAACCTAATGATGAATGCCGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAgg                                                                                                                                                                        >  1:353772/1‑250 (MQ=255)                                                                                   gATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATCGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGCTGACTCTTTACCCCAGCCAGGAGCCGTat                                                                                                                                                                >  2:17958/1‑251 (MQ=255)                                                                                      tAGCACGGGGATAGTTGAGACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTGCCTGTTTGTTTCGCGAGTACGAAACCTGTGGCACGCACCCCGCGTTAGATCGCCCTGCACATGTCGCAGCCATGCTCACTGATAGCGAATTCAATGTCATCGGCGAACCCTATGTCTGTTATTGCAAGCCCGCTGAGGACTAttttccacattcagtgggtatatta                                                                                                                                                              <  2:383033/250‑23 (MQ=255)                                                                                                  atTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAAGAGCCGTATTAATTTCCGGTATTa                                                                                                                                                 <  2:201706/251‑1 (MQ=255)                                                                                                           tctGGCAGACAGCAGAACTAACGGCTTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCACGGAGCCGTATTAATTACCGGTATTACACCGCAgg                                                                                                                                        >  2:481026/1‑251 (MQ=255)                                                                                                              ggCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGa                                                                                                                                       >  1:561733/1‑249 (MQ=255)                                                                                                               gCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGc                                                                                                                                    >  1:578342/1‑251 (MQ=255)                                                                                                                   aCAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAACAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACgg                                                                                                                                <  1:214449/251‑1 (MQ=255)                                                                                                                     agcagAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTGCCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGTCTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGc                                                                                                                              <  2:226068/251‑1 (MQ=255)                                                                                                                      gcagAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTGTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTGCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTAGTACCCCGCAGGAAGCACGGGCg                                                                                                                             <  2:444013/251‑1 (MQ=255)                                                                                                                            aTAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAgg                                                                                                                        >  1:139290/1‑250 (MQ=255)                                                                                                                                           tAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCcgcg                                                                                                        >  1:509391/1‑251 (MQ=255)                                                                                                                                            aaTGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGt                                                                                                       >  1:456139/1‑251 (MQ=255)                                                                                                                                               gatgaATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTg                                                                                                    <  2:578342/251‑1 (MQ=255)                                                                                                                                                            aaGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTc                                                                                        <  1:203097/250‑1 (MQ=255)                                                                                                                                                              gCAACAATCTACCTTTTTGTTTCCCGATTACGAAACCTTTGCCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTAAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTATTTACCGTTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACt                                                                                     >  2:153844/1‑251 (MQ=255)                                                                                                                                                                                  ttCACGATTACTAAACCTTTGGCACGCACCCCGCGTTAGATCGACCTGCACAGTTCGCAGCCATTCGCCCTGATAGCGACTTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGCCGACAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtcccgtaccgc                                                                   >  2:292562/1‑248 (MQ=255)                                                                                                                                                                                             gAAACCTGTGGCACGCCCCCCGCGTTTGATCGCCCTGCACAGTTCGCAGCCAGTCGCACTGATAGCGAATTCAATGTCAGCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCt                                                      <  2:322267/251‑1 (MQ=255)                                                                                                                                                                                               aaCCTTTGGCACGCACCACGCGTTAAATCGCCCTGCACATTTCGCAGCCATTAGCACTCATAGCGAATTCAATCTCATCGGCGAACCAGCAGTCTTTTACTGCAAGCCCGCTGATGTCTATTTACCCCAGCCATGAGCCGTATTAATTACCGGAATTACCCCGCAGAACGCACGGGCGCCAAGAGAACAAGCAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAATACCTGTAATCttg                                                    >  2:479391/1‑249 (MQ=255)                                                                                                                                                                                                          cgcacCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAAt                                         >  1:208434/1‑251 (MQ=255)                                                                                                                                                                                                                    gatAGATCGCCCTGCACAGTTAGCAGCCACTCGCACTGATAGCGAATTCAATGTCATCGGCGCACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGATACGTATTAATTACCGGTATTACCCCGCAGCAAGCACGGGCGAAAGGAGAAAACGAAGCCGCTTTTGCCGCCCGTCTTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcg                               >  2:123368/3‑251 (MQ=255)                                                                                                                                                                                                                             cccTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacga                        >  2:165908/1‑249 (MQ=255)                                                                                                                                                                                                                              ccTGCACAGTTCGCAGCCATTAGCACTGCTAGCGAATTCAATGTCATCGGCGAACCCGACGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGAATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGt                     >  2:574307/1‑251 (MQ=255)                                                                                                                                                                                                                               cTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACACCAATGTGCGTTTCGACGACGAAGTc                    >  2:32310/1‑251 (MQ=255)                                                                                                                                                                                                                                           cAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGTAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGTGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt        <  1:193615/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCTCACGCAACAttttt     <  2:270141/251‑1 (MQ=255)                                                                                                                                                                                                                                                  tCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt  <  2:139290/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    AGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT  >  NC_000913/2082616‑2083106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑