Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,080,405	C→T	G63S (GGT→AGT)	yeeA ←	putative transporter YeeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,080,405	0	C	T	97.5%	127.3 / NA	40	G63S (GGT→AGT)	yeeA	putative transporter YeeA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/19);  minor base G (0/1);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAACTCAT  >  NC_000913/2080163‑2080632                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    ccATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTGGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGGACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACg                                                                                                                                                                                                                             <  1:73616/251‑1 (MQ=255)   aTAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATCCGCTCAAAGGCGCGAGGGACAACGTTACTCAAGAACg                                                                                                                                                                                                                             >  2:421761/1‑249 (MQ=255)         ccGTATCAATTTCACCTGTCTGGGCACCCACAAAAATTGCCAGCGTATCCCCAATCAATAAACCTTTATCCGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGTACATGGCCGCCGCGCACCAGTCTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGGGCGATAAGCCCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCTCGAGGGACAACGTTACTCCAGAACGAGATTGGc                                                                                                                                                                                                                     <  1:1061/251‑1 (MQ=255)                aaTTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGCCAACGTTACTCCAGAACGCGCTTGGCCCCATa                                                                                                                                                                                                               >  1:332112/1‑250 (MQ=255)                       ccTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAATATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATcaccac                                                                                                                                                                                                       >  1:306819/1‑251 (MQ=255)                          gTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCAt                                                                                                                                                                                                    >  1:404889/1‑251 (MQ=255)                            cGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTAAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATgg                                                                                                                                                                                                  >  1:185706/1‑251 (MQ=255)                                       caacaaTTGCCAGCGTCACCCCAATCAATAAACCTAGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGc                                                                                                                                                                                       <  2:45526/251‑1 (MQ=255)                                                                 aatAAACCTTGATGCGGTTTCTTGCCCAGCGCCAGCAAACCTCAAAGGAACATGGCCGCCGCGAACCAGACTAACATCAGCGGTAACGAGATAAACTCAAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTaaaaag                                                                                                                                                             <  1:308297/251‑4 (MQ=255)                                                                    aaaCCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATCAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCg                                                                                                                                                          >  1:91366/1‑251 (MQ=255)                                                                          tGATACGGTTTCTTGCCCAGCGCAAGCCACCCGCAAAGGAACATGGCCGCCGCGCCCCAGACTAACATCAGCGGTAACGAGATTAACCCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATaa                                                                                                                                                     <  2:126967/250‑1 (MQ=255)                                                                          ggatcggggttCTTGCCCAGCGCCAGCCAACCGCAAAGTAACATGGCCGCCGCGCACCAGACTAACAGCCGCGGTAACGAGATAAACTCCAGTTGCAGATCGATAAGACCTAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATaa                                                                                                                                                     <  2:503295/241‑1 (MQ=255)                                                                           gATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATg                                                                                                                                                   >  1:152612/1‑251 (MQ=255)                                                                                 gTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTA‑CATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGaaa                                                                                                                                              >  2:331130/1‑249 (MQ=255)                                                                                  tttCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGCGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGaaa                                                                                                                                              >  2:225248/1‑249 (MQ=255)                                                                                  tttCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGGGCTTTCCGGGATAGTAAACAGGCGGATAATGAGaaa                                                                                                                                              >  2:226753/1‑249 (MQ=255)                                                                                    tCTTGCCAAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGCCTAACATCAGCGGTAACGAGCTTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGCGAAAAGTg                                                                                                                                          >  2:65295/1‑251 (MQ=255)                                                                                       ggcacAGCGCCAGCCAACCGAAAAGGAACATGGCCGCCGCGCACCAGACTAAAATCAGCGGTAACGAGATTAACCCCAGGTGCAGAGCGATAAGACCAAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTGTCCGGGATAGTAAACAGGCGGATAAGGAGAAAAGTg                                                                                                                                          <  2:231215/244‑1 (MQ=255)                                                                                                ccagccaACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAAACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACGCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGGGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGcc                                                                                                                              <  2:404889/251‑1 (MQ=255)                                                                                                 cagccaACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCACCACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCACCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGcc                                                                                                                              >  1:255844/1‑250 (MQ=255)                                                                                                  agccaACCCCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACCCCAGTTGCAGGGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAg                                                                                                                            <  1:419157/251‑1 (MQ=255)                                                                                                                gaccATGGCCGCCGCGACCCAGACTAACATCAGCGGAAACGAGATTAACTCCAGTTGCAGATCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCAGGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTAc                                                                                                              <  2:508150/248‑1 (MQ=255)                                                                                                                     tGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGc                                                                                                         >  2:507159/1‑251 (MQ=255)                                                                                                                        ccgccgCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTGGCAGAGCGATAAGACCAAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGcaca                                                                                                      <  1:5751/251‑1 (MQ=255)                                                                                                                               gcACCAGACTAACATCAGCGGTCACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCa                                                                                                                             >  2:404462/1‑221 (MQ=255)                                                                                                                               gcACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGACCCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCa                                                                                                                             <  1:404462/221‑1 (MQ=255)                                                                                                                                 aCCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCAAAAATCGAACCCAACCCCTTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCGCAATGCGGTaa                                                                                             <  2:523815/251‑1 (MQ=255)                                                                                                                                  ccAGACTA‑CATCAGCGGTAACGAGATTAACCCCAGTTGCAGAGCGATAAGACCTAAAACCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATg                                                                                           <  1:331130/251‑1 (MQ=255)                                                                                                                                    aGACTAAAATCAGCGGTAACGAGATTAACCCCAGTTGCAGAGCGATAAAACCTAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATgg                                                                                          <  2:152612/251‑1 (MQ=255)                                                                                                                                         aacaacAGCGGTAACGAGATTAACCCCAGTTGCAGAGCGATAAGACCGAAAACCGAACCCAACCCCGAACCGCCAATACGCTCAAAGGCGCGAGTGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAAGGAGAAAAGTGAGCAGGAAGGCCAGCGCGAGCCGAGTACCATGCACAAGGCGGTAATGGCgg                                                                                       <  2:378142/249‑1 (MQ=255)                                                                                                                                                                     gtagaagagCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGCCAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGAGAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCGTAATGAc                                                         <  2:255844/246‑1 (MQ=255)                                                                                                                                                                                aTAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAGAGGCGCGAGGGACAATGTTACTGCAGAATGAGATTCGCCCCATAATAACCACCAGGGTGACCAGCGTCCAGGTGCTTTCCGAGCTAGTAAACAGGCGGTTAATGTGAAAAGAGAGCAGGAATGGCAGAGCCACCCGAGAACCATGCACAATGCGGTAATGGCGGCGTACCCGGTTTTCGAACGAGCtttgttacttatctgaca                                               >  1:259251/1‑234 (MQ=255)                                                                                                                                                                                          aaaTCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGaa                                     >  1:458195/1‑250 (MQ=255)                                                                                                                                                                                          aaaTCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGGGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACACTGCGGTAATGGCGGAATAACCGGGTTTCAAACGAGCATAATGACTTATTGgaacgcactaagaa                                     >  2:453064/1‑244 (MQ=255)                                                                                                                                                                                              cGAACCCAACACCGTACCGTCAAGACGCTCAAAGGCGCGAGGGACAACGTGACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCTGCACGCACGCAGAAACTcc                                <  1:501092/251‑1 (MQ=255)                                                                                                                                                                                                       cacCGTACCGACAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTTAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGATACACCGTAATgaga                       <  2:312114/251‑1 (MQ=255)                                                                                                                                                                                                               cGCCAATACGCTCAAAGGCGAGAGGGACAACGTTACTACAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAAAGAATTATCGGCACGCACGCCCAAACTCCGTAATGAGATTaaaaac               >  2:558441/1‑249 (MQ=255)                                                                                                                                                                                                                gCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAAAGAGATCGGAGCCATAATCACCACCATGGCAACCAGCGCCCAGGTGCTTTCCGGGATAGTAAACAGGCGGCTAAAGAGAAACGTGAGAAGGAAAGCCAGCGCGACACGAGTACCATGAACAATGCGGTAATGGCGGTATACCCGGATTTCACGCAGGCTTAATGACTTATCAGCCCGCTCCGAGAAACTCCGTCATGAAATTAAAAgc               >  1:36755/1‑250 (MQ=255)                                                                                                                                                                                                                  cAATACGCGCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTGTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCt            <  1:327966/251‑1 (MQ=255)                                                                                                                                                                                                                            aaaGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAACTCAt  >  2:181117/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    CCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAACTCAT  >  NC_000913/2080163‑2080632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑