Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,506	G→T	V523V (GTG→GTT)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,506	0	G	T	100.0%	119.9 / NA	40	V523V (GTG→GTT)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/14);  total (26/14)

GAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGT  >  NC_000913/2046260‑2046747                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCg                                                                                                                                                                                                                                                 <  1:45430/251‑1 (MQ=255)          agaAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCAc                                                                                                                                                                                                                                        >  1:193174/1‑251 (MQ=255)           gcaGTGAAATCCCTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGAACTGCAAGCTGCCGGTGGCAAAGTGGTCACAACCGGCCAAACTATTCTGGATACCCTGCCGCCTTACCGGCTCACCCGTACGCCAGAAACCGATAACACCTGGCCGATTGCAGTCACCGCAGACGATGACAAAGGCAATTTGTCGACTAGTGCACAGAGCATGGTTGTCGTTCAGGCAcc                                                                                                                                                                                                                                       >  1:303512/3‑251 (MQ=255)           gaAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGCTGTCCAAGGCACTTTGTCGAATCGTGCACAGAGCATGGTTGTCGTTCAGGCAcc                                                                                                                                                                                                                                       >  1:303495/1‑251 (MQ=255)                   aTCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTa                                                                                                                                                                                                                                >  1:423547/1‑250 (MQ=255)                       cTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGcc                                                                                                                                                                                                                            >  1:207331/1‑250 (MQ=255)                        tGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGcc                                                                                                                                                                                                                            >  2:446326/1‑249 (MQ=255)                         ggTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGa                                                                                                                                                                                                                         <  2:587322/251‑1 (MQ=255)                                     aCAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCg                                                                                                                                                                                                              <  1:489980/250‑1 (MQ=255)                                                      tGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGAAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCa                                                                                                                                                                                            >  1:200773/1‑250 (MQ=255)                                                             cTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTg                                                                                                                                                                                    >  1:423849/1‑250 (MQ=255)                                                                 aaCGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAAcg                                                                                                                                                                                <  1:33577/250‑1 (MQ=255)                                                                 aaCGTCGAAGCCACCGCACTGGAAACTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTATGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCCCCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGAACCTACGCTAAGCCAGAAAGCTTCCTCGGT‑ATCGTTAAGTACACAAA‑CATTGAAcgc                                                                                                                                                                               >  2:500944/1‑251 (MQ=255)                                                                       gAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACACTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGACGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTc                                                                                                                                                                         >  1:518605/1‑251 (MQ=255)                                                                       aaaGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTc                                                                                                                                                                         <  2:102255/250‑1 (MQ=255)                                                                         aGCCACCGCAGTGGAAGCGTCCGGTGGCAAAGTGGTCACAGCGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTccc                                                                                                                                                                       <  2:174711/251‑1 (MQ=255)                                                                         aGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGGCATAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACAACTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGGACCCAAA‑CATTGAACGCGGATAccc                                                                                                                                                                       <  2:570162/251‑1 (MQ=255)                                                                                gCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAAGTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGAGTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGTTGAGTCCCATTCAAc                                                                                                                                                                <  2:519670/251‑1 (MQ=255)                                                                                               ggtagCAAAGTGGTCACAACGGGCAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCCGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTCAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGCCttt                                                                                                                                                 >  2:51498/5‑251 (MQ=255)                                                                                                                aaggttaaaaGATTTTCTGTTTACCTTGCTGGCTCCCCGTTTCCCAAGCACGCAAAAAACCGTTAACCCCTGGCCGATGGATGCCGCCGCCGAAAATGTAAAAGGCAATTTGCCAAACGGATAACAGATCTTGGTTGTGGTTCAGCCCCCTACGCTAAGCCAGAAAG‑TTCCCCTGTCATCGTAAAGTACCCAAA‑CATGGAACGCGGTTTCCCACCCAACCGCCACACTGACTTTTATTGCGCATGATgcag                                                                                                                                <  2:542619/244‑1 (MQ=255)                                                                                                                               tCTGGTTCCCCCGCCGGCTTACGGGTTCACCAGTACGCCAAAAACCGATAACACGTTTCCGGGGGAAGTCTCCGCCGAAGAGGTCAAAGTAAATTTGTCGAACGGGGAACAGACTATGGTTGTCGTCCAGGCACCTACGCTAAGCCAGAAAGATTCCTGGGT‑ATCGTTAAGTACCCAAA‑CATTTACCGCGCATCCCCAGTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCtgtt                                                                                                                  <  1:51498/250‑1 (MQ=255)                                                                                                                                    ttACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCggg                                                                                                            >  1:57737/1‑251 (MQ=255)                                                                                                                                      aCCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATCACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGCTGCAGCAGGTAATCCTGTTGTCGGGCt                                                                                                          >  2:53473/1‑251 (MQ=255)                                                                                                                                        cctgcgaccttacgaggtCACCAATAAGCCAGAAAACGATAACACGTGGCCGTTTGAAGTCAACGCCGAAGGTGTCAAAGGCAATTTATCGAATCGTGAACAGAGCGTGGTTATCGTTCAGGCAACTACACTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGGACCCAAA‑CATTGAACGCGGCTTACCATTCAACCGCCACACTGACTTTTATTGCGCATGGTGCAGCAGGTAATCCTGTTGTCGGGCTgg                                                                                                        >  2:377551/18‑251 (MQ=255)                                                                                                                                                    ccGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGAcgc                                                                                            >  2:200439/1‑251 (MQ=255)                                                                                                                                                                    ccAGAAACCGATACCACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGAAATCCTGTTGTCGGACTGGTGCTCTCGACGCGTCACGAAGGTGTTc                                                                             >  2:387020/1‑250 (MQ=255)                                                                                                                                                                              aTAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCAcc                                                                  >  1:431248/1‑251 (MQ=255)                                                                                                                                                                                      tGGCCGATTGCAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTg                                                           >  1:54457/1‑250 (MQ=255)                                                                                                                                                                                       ggCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGAc                                                         >  1:34722/1‑251 (MQ=255)                                                                                                                                                                                           gactGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTTAACAGAGCATGGATGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCCGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCGTTCTGACTgg                                                      >  2:113538/4‑250 (MQ=255)                                                                                                                                                                                           gATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGCAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACCCTGACTTTTATTGCGCATGATGCAGCAGGTACTCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTggt                                                     >  2:268015/1‑250 (MQ=255)                                                                                                                                                                                            atgaaaGTCCCCGCCGAAGATGGCAAAGCCATTTTGTCGAATCGTGACAAGAGCATGGTTGCCGTTCAGGCCCCTACGCTAAGCCAAAAAGATCCCCCGGT‑ATGGTTAAGTCCCCAAA‑CATGGAACGCGGTTTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGTACATCACCCTTTCTGACTGGaa                                                    <  1:219228/247‑1 (MQ=255)                                                                                                                                                                                             ttgaatccccatccgaacaggTCAAAGTCAATTTGTCCATTCGTAAACAGAGCATGGTTGTCGTTCAGGCACCTACCATAAGCCAAAAATATCCCCCGGT‑ATCGTTACGTACCCAAA‑CATTGAACGCGGATTCCAATTCACCCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGaa                                                    <  1:2501/230‑1 (MQ=255)                                                                                                                                                                                               gAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGaa                                                    >  1:472465/1‑248 (MQ=255)                                                                                                                                                                                                aaGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGaa                                                    >  2:225373/1‑247 (MQ=255)                                                                                                                                                                                                       cgccgAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAGGATAATGGTg                                         >  1:224958/1‑251 (MQ=255)                                                                                                                                                                                                         ccgAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGAc                                       >  1:13739/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATCCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAg        <  1:501430/250‑1 (MQ=255)                                                                                                                                                                                                                                        gaaaCAGAGTATGGTTGTGGTTCAGTCACCAACCCAAAGCCAGAAAGATCCCTCGTT‑ATCTTTAA‑TCCCCAAATCATGGAACGCGGATTCCCATTCATCCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACcaca            <  1:308914/245‑1 (MQ=255)                                                                                                                                                                                                                                              gagCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACCCTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTAACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGt  >  1:362928/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGT‑ATCGTTAAGTACCCAAA‑CATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGT  >  NC_000913/2046260‑2046747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑