Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,041,411	G→A	G13S (GGT→AGT)	zinT →	metal‑binding protein ZinT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,041,411	0	G	A	100.0%	100.3 / NA	30	G13S (GGT→AGT)	zinT	metal‑binding protein ZinT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/17);  total (13/17)

CCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATT  >  NC_000913/2041177‑2041653                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   caggCATACACAGATCGCATCAGGTTATGGTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGAGAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCAGTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACGCTGGAGGAAACTGTTTTGGCGAGTCGTCTTTACAAACCGGCTGTGGCTTTAAGTGTCTTTATTGTTAg                                                                                                                                                                                                                                    <  2:516145/249‑1 (MQ=255)                cGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCtttt                                                                                                                                                                                                                      <  2:113935/250‑1 (MQ=255)                           aTGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGtcag                                                                                                                                                                                                           <  2:35997/250‑2 (MQ=255)                                                 cAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTa                                                                                                                                                                                      <  2:220884/249‑1 (MQ=255)                                                    aTCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTTGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACaga                                                                                                                                                                                 <  1:479639/251‑1 (MQ=255)                                                        ccAAACTAATTTATTTTATTTCAATAAGATAGCTAAGTAGAACTGAGAAAGACATGATATCGTTTCATAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACCTCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGATGTTGCTTTAAGTGTATTTATTGTTAGCGCTCCTGCCTTTTCGCCTGGTCATCACTCAACCGGCAAACCCTTAACagctgtc                                                                                                                                                                             >  2:368108/1‑246 (MQ=255)                                                           aaaTAATTTATTTTAATTCAATAAGATAGATAAGTAGAACTGAGAAATCCATGTTTTTTTTTACTAAGAGTTGATGCATCTTGCTATATGTTACAATATAACCTTACACATAATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAAATGTCTGTAGCTTTAAGTGTCTTTATTTTTAGCGTTCATACCTTTTCGCTCGTTCATCACTCACACGGCTAACCCTTACCAGTGGTCgc                                                                                                                                                                           >  2:78684/1‑249 (MQ=255)                                                                atttatttTAGTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACaaaa                                                                                                                                                                     <  1:456923/251‑1 (MQ=255)                                                                                               gAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCact                                                                                                                                      >  1:94373/1‑249 (MQ=255)                                                                                                  cTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGt                                                                                                                                    >  2:56991/1‑250 (MQ=255)                                                                                                   tGAGAAATCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGACGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACTCGGCAAACCCTTCACAGTGGTCGAACACAAAGCTGCTAATGGTGTTTTTGATGATGACAATGTAc                                                                                                                                  >  2:283650/1‑251 (MQ=255)                                                                                                     agaAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACa                                                                                                                                 >  1:192921/1‑250 (MQ=255)                                                                                                                     gTTTCCTAATAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTg                                                                                                                <  1:204862/251‑1 (MQ=255)                                                                                                                          cTAAGAGGTGTTGCATTTTGCTATATGTTCCAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAAATGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCCTGGTCATCACTCACACGGCAAACCCTTAAACGAGGTCGAACAAAAAGCTGCTAATGGAGTTTTTGATGATGCCAATGTACAAAACAGAACGCTCAGTGACTgg                                                                                                           >  2:386271/1‑251 (MQ=255)                                                                                                                               agTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATgg                                                                                                      >  1:297160/1‑251 (MQ=255)                                                                                                                                     ttcATTTTTCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTGTTGGCGATTCGTCTTTACAAACTTGCTGTTGCTTTAAGTGTCATTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGTTCGAACAAAAAGCTGCTAATTGTCGTTTTGATGATTCCAATGTACAAAACCGGACGCGCAGTGACTGGGATGGAGTcg                                                                                                 <  2:562276/248‑2 (MQ=255)                                                                                                                                          tttGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTTCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACAGAACGCTCAGTGACTGGGATGGAGTCTGGCAAt                                                                                           >  1:156757/1‑251 (MQ=255)                                                                                                                                             gCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCg                                                                                        <  1:30183/251‑1 (MQ=255)                                                                                                                                                        cAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCttt                                                                              >  2:287384/1‑250 (MQ=255)                                                                                                                                                                cATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACGGCAAAg                                                                     <  1:441636/251‑1 (MQ=255)                                                                                                                                                                     cacaTCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGc                                                                 >  1:218759/1‑250 (MQ=255)                                                                                                                                                                          catATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACtt                                                           <  1:395026/251‑1 (MQ=255)                                                                                                                                                                                          ggaggaAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCag                                            <  1:410420/250‑1 (MQ=255)                                                                                                                                                                                             ggaAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCaga                                           >  1:316846/1‑248 (MQ=255)                                                                                                                                                                                              gaAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAgaagaa                                       <  2:560037/251‑1 (MQ=255)                                                                                                                                                                                                    gTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGa                                 <  1:280331/251‑1 (MQ=255)                                                                                                                                                                                                        tGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTCAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATgg                                                                                                                                                         >  2:503769/1‑127 (MQ=255)                                                                                                                                                                                                        tGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCGCTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATgg                                                                                                                                                         <  1:503769/127‑1 (MQ=255)                                                                                                                                                                                                                                  gTTGCTTTAAGTGTCTTTATTGTTAGCGCACCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACAGTTGCTgta     <  2:103957/249‑3 (MQ=255)                                                                                                                                                                                                                                    tGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAAtt  <  2:418725/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   CCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATT  >  NC_000913/2041177‑2041653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑