Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,948,824	C→T	A567T (GCA→ACA)	aspS ←	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,948,824	0	C	T	97.6%	261.3 / NA	83	A567T (GCA→ACA)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (43/38);  minor base A (1/1);  total (44/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

AGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAA  >  NC_000913/1948584‑1949065                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   aGATGAGCACTAAGAGCGAAACGGGACGATTAGACACTTTATACTGCACGCTTCCTCGTCGGTGACTGCCTTCGAGCAGCCCATTCACATAGTTATCTATGCTCATGGGAGTGCACTCAGTTGCCGACTCGATGCAACGCGAAGGATGTCGTGTATTTGAGTCAGATCAGTTATTCTCAGCCTTCTGCACA‑ACCTGAATGCGCAGCGCAGCCAGTGGAGTCGGGTTAGCAAAGCTCGGTGTTTCAGttat                                                                                                                                                                                                                                           <  2:188851/250‑4 (MQ=255) aGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCGCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGtcat                                                                                                                                                                                                                                           <  2:188852/250‑1 (MQ=255)   aTGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCGCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCGCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTCCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAg                                                                                                                                                                                                                                        <  1:578570/251‑1 (MQ=255)     gACCACTCAGATCGAAGAGAGACGCTTATACACTTTATCCTTCACGCTGCCTACTCGATGACTGCATTCGCTCATACCATTCACATAGTTATCAATGCTAATGGGCGTTCAATCAGATGACGCCACGATGCAAAGCGAATGTTTTCGTGTATTTGAGACATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAACGCTCGGTGTTTCAGTCATCAGac                                                                                                                                                                                                                                      >  2:586954/1‑251 (MQ=255)       ccACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGacac                                                                                                                                                                                                                                    <  1:220469/251‑1 (MQ=255)          cTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCt                                                                                                                                                                                                                                 >  1:79592/1‑251 (MQ=255)          cTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCt                                                                                                                                                                                                                                 >  2:185771/1‑251 (MQ=255)           tAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTg                                                                                                                                                                                                                                >  2:510698/1‑251 (MQ=255)                 cGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGccgccg                                                                                                                                                                                                                          >  1:422134/1‑251 (MQ=255)                 cGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGGTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATGTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGGCATCAGACACGCTGccgccg                                                                                                                                                                                                                          <  1:587008/251‑1 (MQ=255)                     aCGGGACGCTTATAATCTTTATCCTTCACGCTGCCTCTCCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCACAGCTCGGTGTTTCACTCATCAGCCACGCTGCATCCGTGGt                                                                                                                                                                                                                      >  2:319205/1‑251 (MQ=255)                      cgggacgctcagcctctatacccgtCACGCTGCCTCCACGTTGACTGTCGTCGCTCATCCCATTCACACAGTTATCTATGCTCATGGGAGTTCTCTCAGTTGCCGCCTCGATGCCCCGCGAATGATTTCGTGTATTTGATTCATATCATTTATTCTCAGCCTGCGTCACA‑ACCGGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGATTCATCCATCAGACACGATGCCGCCGTGGtt                                                                                                                                                                                                                     <  2:246933/227‑1 (MQ=255)                       gggACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGttt                                                                                                                                                                                                                    >  1:61990/1‑251 (MQ=255)                        ggACGCTTATACACTTTATCCTTCTCGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATGCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCTTGTATTTGAGTCATATCAGTTATGCGCAGCCGTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTATGAAAGCTCTGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGtttt                                                                                                                                                                                                                   <  2:529682/251‑1 (MQ=255)                          aCGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCCCATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGGGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCg                                                                                                                                                                                                                 >  2:307635/1‑251 (MQ=255)                            gCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGAATGCCTTCGCTCATACCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGACTGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCCAAGCTCGGGGTTTCAGTCATCAGACACGCTGCCGCCTTGGTTTTCggg                                                                                                                                                                                                               >  2:477702/1‑251 (MQ=255)                               tataCACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGTTGCCGCCGTGGTTTTCGGGaa                                                                                                                                                                                                             <  2:312401/250‑1 (MQ=255)                                    acTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGAt                                                                                                                                                                                                       >  1:78727/1‑251 (MQ=255)                                      tttATCATTCAAGCTGCCTCTTCGCTGCATGCCTTCGCTCATCCTATTAACCTAGTTATCTATGCTCCTGGGTGTTCACTCAGTTGCCGCCTCGATGCACAGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCCGTGCCGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGCAAGGCGATa                                                                                                                                                                                                      >  2:70090/1‑250 (MQ=255)                                        tATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACg                                                                                                                                                                                                   >  2:65737/1‑251 (MQ=255)                                              tCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCAc                                                                                                                                                                                               <  1:350769/249‑1 (MQ=255)                                              tCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACg                                                                                                                                                                                              <  2:438358/250‑1 (MQ=255)                                                 cGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGat                                                                                                                                                                                           >  1:139141/1‑250 (MQ=255)                                                    tGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTg                                                                                                                                                                                       >  1:527882/1‑251 (MQ=255)                                                     gCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATATTTATCTATGCTCATGGGAGTTCCCTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTg                                                                                                                                                                                       <  2:422134/250‑1 (MQ=255)                                                        tctTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑Cgg                                                                                                                                                                                  >  2:4849/1‑251 (MQ=255)                                                          ttCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGAATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTTCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTg                                                                                                                                                                                >  1:565571/1‑251 (MQ=255)                                                                     ccTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTcagcag                                                                                                                                                                     <  1:513026/251‑1 (MQ=255)                                                                      cTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCagcagc                                                                                                                                                                    >  1:357257/1‑251 (MQ=255)                                                                         cGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATACCGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATg                                                                                                                                                                 >  1:354465/1‑251 (MQ=255)                                                                                cccATTCACGTAGTTCTCTATGCTCATCGGAGTTCACTCAGATGCCGCCACGATGAACCGCGAATGTTTTCGTAAATTAGAGTAATATCAGTTATTCTCAGCCTTCTTCCCA‑ACCTGAATGATCAGCTCAGCCAGTGCCGTCGGGCTAGCAAAGCTCGATGTTTCCGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAAAGTCACGGATATTGT‑CGGGGTCGGTCCGCAGGATGGTCAGa                                                                                                                                                           >  2:239645/1‑250 (MQ=255)                                                                                cccATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGAc                                                                                                                                                          <  1:555618/251‑1 (MQ=255)                                                                                   aTTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGt                                                                                                                                                       >  1:217888/1‑251 (MQ=255)                                                                                   aTTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTAAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGGTTTCGGGAAGGCGATAACGTCACGGATATAGTACGG‑GCCGGTCAGCAGCCAGGTCAGACGGt                                                                                                                                                       >  2:282277/1‑251 (MQ=255)                                                                                    ttCACATAGTTATCTATGCTCATGGGAGCTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTATCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGGTTTCGGGAAGGCGATAAAGGCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGCCAGCCGGTc                                                                                                                                                      >  2:579880/1‑251 (MQ=255)                                                                                           aGTTATCTATGCTCATGGTAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTGCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCg                                                                                                                                               <  1:105213/251‑1 (MQ=255)                                                                                             ttATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATCTCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGAGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTAGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGa                                                                                                                                              >  1:35300/1‑250 (MQ=255)                                                                                              tATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAAt                                                                                                                                            >  1:168298/1‑251 (MQ=255)                                                                                                tctctGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGA‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGCATGc                                                                                                                                          >  2:382777/5‑251 (MQ=255)                                                                                                         aTGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCtgc                                                                                                                                 >  2:69891/1‑251 (MQ=255)                                                                                                            ggAGTTCCCACAGTTGCCGCCGCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCtgcgtg                                                                                                                              <  2:401376/251‑1 (MQ=255)                                                                                                            ggAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCa                                                                                                                                                             <  1:424110/220‑1 (MQ=255)                                                                                                            ggAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCa                                                                                                                                                             >  2:424110/1‑220 (MQ=255)                                                                                                            ggAGTTCACTCAGTTGCCGCCGCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCGTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCtgcgtg                                                                                                                              <  1:425822/251‑1 (MQ=255)                                                                                                              aGTTCACTCAGTTGACGCATCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCGGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCCGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCtgcgtgcg                                                                                                                            >  1:504226/1‑251 (MQ=255)                                                                                                               gTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTgcgg                                                                                                                           <  1:510698/251‑1 (MQ=255)                                                                                                                  cACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTgcggcg                                                                                                                         <  1:65737/250‑1 (MQ=255)                                                                                                                   aCTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCAGCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATATCGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGa                                                                                                                       <  1:273216/251‑1 (MQ=255)                                                                                                                       aGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTAc                                                                                                                   >  1:405408/1‑251 (MQ=255)                                                                                                                       aGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAAGGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTAc                                                                                                                   <  1:429410/251‑1 (MQ=255)                                                                                                                          ggccgtcTCGATGCAACGCGAATGATTTCGTGTATTTTGGTCATATCAGTTATTCTCAGCCTTGTGCACA‑ACCTGTAGGCTGAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTGGGTGTTTCAGTCATCAGACACGCTGCCGCCGGTGTTTTCGGGAGGGCGATAAGGTCTCGGATAGTGT‑CGGTGCCGGGCAGTAGCATGGGCAGACGGACAAGACCGAATGCCTTACGGGCGTGCGGCGGGGGACCGt                                                                                                                <  2:30909/250‑1 (MQ=255)                                                                                                                                     tGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGAc                                                                                                                                                          <  2:157439/198‑1 (MQ=255)                                                                                                                                     tGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGAc                                                                                                                                                          >  1:157439/1‑198 (MQ=255)                                                                                                                                       cAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTc                                                                                                   >  1:218756/1‑251 (MQ=255)                                                                                                                                           gcgAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCGCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGc                                                                                               <  2:78727/251‑1 (MQ=255)                                                                                                                                            cgAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCa                                                                                              <  1:62052/251‑1 (MQ=255)                                                                                                                                             gAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAg                                                                                             <  2:139141/251‑1 (MQ=255)                                                                                                                                                 gATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGa                                                                                           >  1:208300/1‑249 (MQ=255)                                                                                                                                                       gtgtATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGACAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAAt                                                                                   >  1:69611/1‑251 (MQ=255)                                                                                                                                                          tATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGGCGGGTTAGCAAAGCTCGGTTTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATTGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAAttt                                                                                 <  1:345694/250‑1 (MQ=255)                                                                                                                                                             ttGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTtctc                                                                              <  1:322946/250‑1 (MQ=255)                                                                                                                                                                      atCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTcc                                                                    >  1:92982/1‑251 (MQ=255)                                                                                                                                                                      atCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTcc                                                                    >  1:82120/1‑251 (MQ=255)                                                                                                                                                                        cAGTTATTCTCAGCCTTCTTCCCA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCTTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATATCTCGCGCTGTTCctc                                                                  <  2:218756/251‑1 (MQ=255)                                                                                                                                                                         aGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCctct                                                                 >  1:485198/1‑251 (MQ=255)                                                                                                                                                                         aGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGAGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGCAGCCGAATTTCTCGCGCTGTTCctct                                                                 >  1:54035/1‑251 (MQ=255)                                                                                                                                                                         aGTTAGTCTCAGCCGTCTTC‑CATACCTGAGTGTCCAGCGCAGTCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGGCATCAGACAGGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGGCAGACGGTCAAGAGCGAATGCCAGACCTGCGTGCGGCGGAGGACCGTACTTCAGAGCGTCGAGCAGGATGCCGAATTGCTCGCGCGGTTccgct                                                                 <  2:273227/251‑4 (MQ=255)                                                                                                                                                                          gTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCCGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCtt                                                                >  2:408189/1‑251 (MQ=255)                                                                                                                                                                            tATTCTCAGCCGTCTTCACA‑ACCTGAATGCTCAGCGCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTTCTCTTc                                                               <  1:258599/250‑1 (MQ=255)                                                                                                                                                                             aTTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTGCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTGCTCTTCg                                                              <  2:34384/250‑1 (MQ=255)                                                                                                                                                                               tctcAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGACAGTGCAGTCGGGTTAGCAAATCTCGGTGTTTCAGTCATCAGACACGATGCCGACGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGCCGGTCAAAACCGAATGGCAGACCTGCGTGCGGCGGAGAACCGTCTTTCAGAGAGTCGAGCAGGAAGCCGAATTTCTCGCGCTGCTCCTCTTCGTTg                                                           >  2:280623/1‑251 (MQ=255)                                                                                                                                                                                                     gAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACAcc                                      <  1:69891/251‑1 (MQ=255)                                                                                                                                                                                                       aTGCGCAGCTCATCCAGTGCAGGCGGGTTAGCAAAGCTCGGTGTGTCATTCAGCAGACAGGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCCCGGATATTGT‑CTGCGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGCCCTTTGTGCGGGGGATGACCGTATTTCAGAGCGTCGAGCATGAAGCTGAATTTCGCTCGCCGTGCCTCTTCTTTGAttcgtagattgtcatataacgt                                    <  2:75523/251‑22 (MQ=255)                                                                                                                                                                                                            cagctcagcCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTctgct                               <  1:426178/251‑1 (MQ=255)                                                                                                                                                                                                              gctcagcCAGTGAAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGCTAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGGCCCGTATTTCAGAGCGTCGAGCAGGAAGCCGACTTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCtgctgc                             >  2:353584/1‑251 (MQ=255)                                                                                                                                                                                                                 cagcCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGCTACCCAGAATACCAAACACCGTCTGCTGCat                           >  2:535889/1‑250 (MQ=255)                                                                                                                                                                                                                 cagcCAGTGCAGTCGGGTTAGCAAAGCTCGGCGTTTCAGTAATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGAAGGTCAAGACCGAATTCCAGACCTGCGTGCGGCGGAGTACCGAATTTCAGAGCGTCGAGCAGGAACCCGAATTTTTCGAGCTGTTCCTCTTCGTTGATACCCCGACAACCAAACACCTTCTGCTGCat                           >  2:444610/1‑250 (MQ=255)                                                                                                                                                                                                                         gCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCAt                   <  1:4849/250‑1 (MQ=255)                                                                                                                                                                                                                           aGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTAt                <  1:22701/251‑1 (MQ=255)                                                                                                                                                                                                                               gggTTAGCAAAGCTCGGTGATTCAGTCCTCAGACACGCTGCCGCCGTTGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCCTGGCCAGACGGTCAAGACCGCATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCCAAGCGTCGAGCAGGAAGCCGACTTTCTCGCGCTGATCCTCTTCGTTGATAACCAGAATACCCAACACCGTCTGCCGCATATCACCATTATGGAt            >  2:27151/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACg         >  1:205570/1‑251 (MQ=255)                                                                                                                                                                                                                                   tAGCAAAGCTCTGTTTTTCAGTCCTCAGACACTCTGCCGCCGGGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGTTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGGATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTTATACCCAGAAGACCAAACACCGTCTGCTGCATATCACCATTATGGATACg         <  2:25012/250‑1 (MQ=255)                                                                                                                                                                                                                                         aGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGaa  <  1:408189/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   AGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACA‑ACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGT‑CGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAA  >  NC_000913/1948584‑1949065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑