Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,068	A→G	V45A (GTA→GCA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,068	0	A	G	100.0%	105.0 / NA	36	V45A (GTA→GCA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/21);  total (15/21)

GGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTGATAAGT  >  NC_000913/155834‑156310                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGCTACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATAGCCAGTATCTAACCAACTCTGGCCAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTg                                                                                                                                                                                                                                    >  1:516328/1‑251 (MQ=255)              cTTCCAGTACGTTACACCAGACCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCCGACGTGCGTTGACACTTTTTTGATCGCTTTtatat                                                                                                                                                                                                                       >  2:87238/1‑251 (MQ=255)               ttCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGATGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCGGGCACAGCGTTGTCAACGCCATGATCTAACCAACTCTGGACAAGAAACGGGTTATTCCCTTTATTTACCAGACGTGCATTGACACTTTTTAGATCGCTTTtatat                                                                                                                                                                                                                       >  1:258273/1‑250 (MQ=255)                 ccAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTTGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTGTTTGATCGCtttt                                                                                                                                                                                                                           <  2:414400/244‑1 (MQ=255)                 ccAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCtttt                                                                                                                                                                                                                           >  1:414400/1‑244 (MQ=255)                      aCGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCACTGAGGAACTCGCTGCGTACATTACTTTGATTGTGTGCCCACGATTCGCATGAAAACGAGCTGCTGCCGGCGCAGCAGGAAATGAGCCTGCTCA‑ACCGCCAGGCTCAGCGTTATCATCACCAGTATCTAACCAACGCTAGACAAGCACAGGGTTACGCCCTTTATTTTCCAGACAAGCGTTGACACGTTTTTAATAGCTTTTATCTATTAcgtg                                                                                                                                                                                                               >  1:29178/1‑249 (MQ=255)                        gTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTAcgcg                                                                                                                                                                                                               >  1:198321/1‑249 (MQ=255)                           aaaCCAGCACACGCTCTCTATGTATTTAGAACGTGCGGTACTGCCTGTGTACATTAATTTGATTGCTTGCCCACATTTGGCATCACTGCGCGATACTGGCGGCGTCGCAGTAAAACGGACTG‑TAATACTGCCAGGCTCAGCGCTGTCCTCGCCAGAATCTAACAAACTCTGGAAACGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGCCACTTTTTTGATCGCTTTTATATATTAAGCGAGTAc                                                                                                                                                                                                          >  2:241379/1‑251 (MQ=255)                               cAGAACACGCTCTCTCTGTCGTTAGGCAGTGATGTGCTGGTTGTGTACATTAATTGGATTGTTTGCCCACGTTTGGCATCAATACGCGACACTGGCGGCGTAGCAGTAAAAGGGTCTG‑TAATACTGCCAGGCTTAGCGTTGTCATCGCCAGTATCTAGCCAGCTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGa                                                                                                                                                                                                      <  2:582316/251‑1 (MQ=255)                                  aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAAt                                                                                                                                                                                                   <  1:207453/251‑1 (MQ=255)                                    cacGCTATCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATg                                                                                                                                                                                                  >  1:152736/1‑250 (MQ=255)                                            ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGt                                                                                                                                                                                           <  2:462479/249‑1 (MQ=255)                                                         cAGTGAGGTGCTGGCGGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTGTTTGATCGCTTTTATATATTACTCGAGTACCCGAAATGACAATGTCCGCAATAGACGATg                                                                                                                                                                            <  2:135309/251‑1 (MQ=255)                                                                      gctgTGTACATTAATTTGAGTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACGCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCAt                                                                                                                                                               <  1:19285/251‑1 (MQ=255)                                                                       ctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCAt                                                                                                                                                               >  1:514229/1‑250 (MQ=255)                                                                         gtgtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACa                                                                                                                                                            <  2:549317/251‑1 (MQ=255)                                                                                    tttGATTGTTTGCCCACGTTTAGCATCACTCCGCGATAATGGCGGAGTAGCAGTAACAGGGACTG‑TAATACTGCCACGCTCAGCGTTGTCCTCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGGGGACCCGAAATGACAATGTCCGCACTTGACGATGAACTAAAAGCCATACAGGATACGAAGc                                                                                                                                                 >  2:104896/1‑251 (MQ=255)                                                                                       gATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCAt                                                                                                                                               <  1:308896/250‑1 (MQ=255)                                                                                           gTTTGCCCACGTTTGGCATCAATACGCGATTCTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCCCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGAAAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAg                                                                                                                                           <  1:404351/250‑1 (MQ=255)                                                                                                cccACGTTTGGAATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtgttg                                                                                                                                     <  2:52667/251‑1 (MQ=255)                                                                                                 ccACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGACATACAGGTTACGAAGCATAAAGCtgttgt                                                                                                                                    >  1:316393/1‑251 (MQ=255)                                                                                                       ttGGCATCAATACGCGATCCTGGCGGCGTTGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCTCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCTGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGtt                                                                                                                               <  1:41639/250‑1 (MQ=255)                                                                                                                   cgcgATACTGGCGGCGTAGCAGTAAAATGGACTG‑TAATACTGCCAGGCTCAGCGTTTTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACTTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACTATGAACTAAAATCCAAACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaaaaaa                                                                                                                  <  2:192341/251‑1 (MQ=255)                                                                                                                                                    g‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAAc                                                                                  <  2:166001/250‑1 (MQ=255)                                                                                                                                                             gCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGAGCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGaata                                                                          <  1:504611/250‑1 (MQ=255)                                                                                                                                                                        cGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGa                                                              <  1:228950/251‑1 (MQ=255)                                                                                                                                                                                             aCCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATatt                                         <  1:304248/251‑1 (MQ=255)                                                                                                                                                                                              ccAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACCCTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAggg                                                                              >  2:78409/1‑213 (MQ=255)                                                                                                                                                                                              ccAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTGTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAggg                                                                              <  1:78409/213‑1 (MQ=255)                                                                                                                                                                                                      ggACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGTCGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATtcaatc                                <  2:11157/251‑1 (MQ=255)                                                                                                                                                                                                               aCGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACt                       <  1:531564/251‑1 (MQ=255)                                                                                                                                                                                                                    ttATTCCCTTTATTGTCCCGACGTGCGTTGACACTTTTTTGCTCGCTTTTTTATATTACGCGAGTACCCGACATGCCACTGCCCGCAATAGACGATGACCTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATACCTGATAGAGTTAATTAAACCAGTGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTg                   >  2:233548/1‑250 (MQ=255)                                                                                                                                                                                                                      aTTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAAt                >  1:269050/1‑251 (MQ=255)                                                                                                                                                                                                                               aTTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTg        <  1:424532/250‑1 (MQ=255)                                                                                                                                                                                                                                  ttCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTGATa     <  1:119788/250‑1 (MQ=255)                                                                                                                                                                                                                                     cAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTGATAAGt  >  2:327095/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   GGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTG‑TAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTGATAAGT  >  NC_000913/155834‑156310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑