| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,903 | G→A | E523K (GAA→AAA) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,903 | 0 | G | A | 94.5% | 233.1 / ‑3.0 | 73 | E523K (GAA→AAA) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base G (1/3); new base A (35/34); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCG > NC_000913/1660‑2138 | gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACGTGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACt < 1:152025/251‑1 (MQ=255) aaaTAACGATGATTCGACCACTTGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTTC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTTGCAGAAAG‑AACTTgcg < 1:517500/250‑1 (MQ=255) aaaTAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATCCCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGgcg < 1:517518/250‑1 (MQ=255) aaaTAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGGGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGgcgc > 1:232546/1‑251 (MQ=255) aaaTAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGGTATCGAAGTGTTTGTGATTGGCATCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGCAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTTGAAAACTGGCAGAAAG‑AACCGgcgc > 2:149342/1‑251 (MQ=255) aaCGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAg < 2:131127/250‑1 (MQ=255) gatgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCaa > 1:303440/1‑248 (MQ=255) gACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGt > 2:160730/1‑251 (MQ=255) gACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGAGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGGGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGt > 1:478130/1‑251 (MQ=255) aCTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTAAGTGTCTGCGGTGTTGC‑CACATCGAAGGCTCTGCTCCCCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTa > 1:520833/1‑250 (MQ=255) cTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAAt > 1:515102/1‑251 (MQ=255) ggCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTCTCGAAGTGTTTGTGATTGGCGTCGGCGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACCTATCGACTTACGCGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTAAACAATGTACATGGCCTTAATATGGCAAACTGGAAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAAtct > 1:395515/1‑251 (MQ=255) ggCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGATCCGTTTAAt < 2:36130/249‑1 (MQ=255) ggcgcGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCggg < 2:205281/250‑1 (MQ=255) gcgcGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGATCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGgc < 1:46700/251‑1 (MQ=255) gTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCtt > 1:313570/1‑251 (MQ=255) tGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTg > 1:162148/1‑251 (MQ=255) cccGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGGTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCAAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatca < 2:406985/250‑1 (MQ=255) ccGAGCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAAGCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatcat < 1:69576/251‑1 (MQ=255) cGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCACAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatcatc > 1:188113/1‑251 (MQ=255) gatccgggtATCAAAGTGTTTGTGATTGGCAACGTGGGCGTTGGCGGGACGCTGCTGGAGCAATTGAAGCGTCAGTAAAGGTGGCTGCTGAATTAACATATAGCATTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCCGTTCCCCAATGTACATGGCCTTAATCCGGACAACTGGCAGGACG‑CACTGGCGCACACCAACGACCCGTTTAATCCCCGGCGCTTAATTCGCCTAGTGGAAGAATatcatcg > 2:214980/9‑250 (MQ=255) aTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCTCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATctg > 2:104450/1‑251 (MQ=255) cAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATctgct < 1:523986/251‑1 (MQ=255) ggTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGa < 1:59802/251‑1 (MQ=255) cGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCgg < 2:313570/251‑1 (MQ=255) aGTGTTTTTGATTGGCGGCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCa < 2:310309/251‑1 (MQ=255) tgtTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGAAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCAtt > 2:292577/1‑251 (MQ=255) ttGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCAttgtt > 1:146155/1‑251 (MQ=255) ttGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACAGGGCCTGAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCGCGGGCGTTTAATGCGCCTCGTGAAAGAATATCATctgcggtactcgtgcattgt < 2:252172/250‑17 (MQ=255) cGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCGTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACtt < 2:104779/251‑1 (MQ=255) cGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCCGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGCTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGACAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTcc > 1:539450/1‑250 (MQ=255) gTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTccagc < 1:11806/251‑1 (MQ=255) gTGGCGTTGGCCGTGAGCTGCTGGCGCCACTGAAGCGTCAGCAACGCTGGCTGAAGACTAAACATCTCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCGGCTAACCAATGTACAAGGCCTTAATCTGGAACACTGGAAGATAG‑AACTGGCGCAAGCCAAAGAGCAGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATATTATGCTGAACCCGGTCAATGTTGATTGCACTTccagc > 2:36979/1‑251 (MQ=255) tGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTccagcc > 2:166620/1‑251 (MQ=255) tGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTccagcc > 1:110167/1‑251 (MQ=255) gCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTccagccag < 1:289812/251‑1 (MQ=255) cGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGACTTAATCTGGAAAACTGGCAGAAAG‑ACCTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCACCCAgg > 2:158629/1‑251 (MQ=255) ggtGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTTCGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAATCTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGTGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGc < 1:556671/249‑1 (MQ=255) gTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGACTCGTGAAAGCATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGc > 1:533408/1‑251 (MQ=255) ggCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGt < 1:160730/251‑1 (MQ=255) cgcTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCggtt > 1:340811/1‑249 (MQ=255) ggAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTTATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAatat < 2:546192/250‑1 (MQ=255) ggAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATg < 1:173143/251‑1 (MQ=255) cAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGa < 1:521955/251‑1 (MQ=255) gAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATATGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTcc > 1:293012/1‑251 (MQ=255) cgacAGCAAGGCTGGCGGAAGAATAAACATGTCGAGTATCGGGTCGGCGGTGT‑GCTCACCTAGCAGACTCTGCTCACCAATGTTCATGGCCGTAATCTGGAAAACTGGC‑TGAAGTAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTGATTCGCCTCGTGAAAGAAGATCGTCTGCTGAACCCGGTCATGGTTGGTTGCTCTTCCTGATAGGCAGTGGGGGATCAAGATGCCGACGTCCTgcg < 2:566229/248‑1 (MQ=255) gCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAg < 2:75802/251‑1 (MQ=255) aaaGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGt > 1:246813/1‑251 (MQ=255) gttgctgAAGGATAGACATTTCGACTTACGTGTCGGCGGTGTTGC‑CGATTTGAAGGCGCGGGTCACCAATGTACATGTCCGTAATCTGGAAAACTGGGAGGAAG‑AACTGGCGCAAGACAAAGTGCCGTTTAAGCTCGGGCGCTTAATTCGCCTCGTGAAAGAAGATCAGCTGCGGAGCCCGGGCATTTTTGTGTGGGCTTCCAGGCAGGCAGTGGCGGATCAATATGCGGACGTCCTGCGGGAAGGTTTcc < 2:73754/248‑1 (MQ=255) ggctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTc < 1:368347/147‑1 (MQ=255) ggctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTc > 2:368347/1‑147 (MQ=255) gctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGA‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCAGTCATTGTTGACTGCACTTCCAGCCAGGACGTGGCGGATCAACGTGCCGACTTCCCGCGCGACGGTTTCAACg > 2:487461/1‑251 (MQ=255) gaaTAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCa < 1:491539/251‑1 (MQ=255) atatCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGTAGCAATATGCCGACTTCCTGCTCGAAGGTTTCCTCGTTGTCACGCCGAAc < 1:553626/251‑1 (MQ=255) ttACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGc > 2:444018/1‑251 (MQ=255) gtCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAaca < 1:66191/250‑1 (MQ=255) gtCTGCGGGGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCAGGAAAACTGGCAGAACG‑AAATCGCGCAAGACAAAGAGCCGTTTCATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGCTCAATATGGCGCCTTCCTGCGCGAAGGCTTCCACGGTGTCACGCCGACAAAAAAGGGCAacac > 2:337963/1‑251 (MQ=255) ggTGTTGC‑CAACTCGAAGGCTCTGCTCACCAAGGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCtcgtc < 2:232546/251‑1 (MQ=255) gC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGCTCAATTTGCCGACTTCCTGCGCGAAGGGTTCCACGGTGTCACGCCGAACAAAAAGGCCAACACATCGTCGATgg > 1:50502/1‑250 (MQ=255) aaCTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGACGCCTTCCTGCGCGAAGGGTTCCACGGTGTCAAGCCGCACAAAAAGGCCAACACATCGTCGATggctt > 2:480865/1‑247 (MQ=255) aCTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtac < 1:374403/251‑1 (MQ=255) tctGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGt > 1:453373/1‑251 (MQ=255) ctGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATTCCGGCTTCCTGCGCGAAGGTTTCCACGTTGTTCCGCCGACCAAAAAGGCCAACACCTCGTCGATGGATTAATCCCATCaagt > 2:413162/1‑248 (MQ=255) gCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGtt < 1:443712/249‑1 (MQ=255) cACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGtt > 1:525782/1‑251 (MQ=255) ccAATGTACATGGCCTTAATCTGGAAAACCGGCAGGTAT‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCACTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGAGCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTTCCAGCAGTTGCGTTag < 2:514197/251‑2 (MQ=255) cAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATg > 1:342296/1‑251 (MQ=255) aTGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGATTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATAAATATGCCGACTTCCTGCGCGAAGGGTTCCACGTTGTCACGACGAACAAAAAGGCCAACACCTCGTCGCTGGATTACTTCCATCCGTTGCGTTATGCGGCGGa > 2:428681/1‑250 (MQ=255) tGGCCTTAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGGATTCCTGCGCGAAGGTTTCCCCGTTGTCACGCCGAACAAAAAGGCCCACACCTCGTCGATGGCTTTCTACCATCCGTTGCGTTATGCAGCGGaa > 2:3577/1‑250 (MQ=255) ggCCTGAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATAGCATCTGCTGAACCCGGTCATTGTTGACTGCACGTCCAGCCAGGCAGTGGCGGATCAAGATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGGTGCGTTATGCGGCGGaaa < 2:515102/250‑1 (MQ=255) tAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATcg < 2:146155/250‑1 (MQ=255) tAATCTGGAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATc < 1:464213/249‑1 (MQ=255) ggAAAACTGGCAGAAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCgcggcg < 1:544117/249‑1 (MQ=255) | GTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGC‑CAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAG‑AACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCG > NC_000913/1660‑2138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |