Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,760,384	0	A	C	25.6%	108.4 / 15.4	89	V289G (GTG→GGG)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand):  ref base A (42/22);  new base C (3/19);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 3.83e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTAAACGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATAATCATTCGCG  >  NC_000913/3760136‑3760629                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        gTAAACGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑GACATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCCAACCAAT‑TGT‑C‑GCCGGAGAAACTCAcc                                                                                                                                                                                                                                                       >  2:484094/1‑251 (MQ=255) gTAAACGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑GGT‑C‑GCCGGAGAAACTCAc                                                                                                                                                                                                                                                        <  1:409625/250‑1 (MQ=255)   aaaCGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCATAACAACT‑TGT‑C‑GCCGGAGAAACTCACCa                                                                                                                                                                                                                                                      <  1:85374/250‑1 (MQ=255)      cGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACt                                                                                                                                                                                                                                                 <  1:212041/251‑1 (MQ=255)          gtgCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGAcc                                                                                                                                                                                                                                             >  1:339332/1‑250 (MQ=255)           tgCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑CATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATTCTCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑CGCGGCCCGCCTAACAACT‑TGT‑T‑GACGGAGAAACTCACAC‑GACTGAcgc                                                                                                                                                                                                                                            >  2:280481/1‑251 (MQ=255)            gCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑GTCATCTCTTTTTTACC‑CACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCCGAGAAACTCAACC‑GCCTGAcgcc                                                                                                                                                                                                                                           >  2:28868/1‑251 (MQ=255)             catGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCCGGGAAACACCCCC‑GACTGAcgccc                                                                                                                                                                                                                                          >  2:196823/3‑251 (MQ=255)                     ggaatgactgcggcgctgcgGGTAAGCGGGCGCAGGGTCGTTAATTTTTCACTCAGGGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AAGGC‑AC‑GCTGCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ACCACCGCTTTTTTACC‑AACAA‑GAATCCCGGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAAt                                                                                                                                                                                                                                  <  1:79222/239‑1 (MQ=255)                       aaTGACCTCGGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGTTCATTTTACCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACACCT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCa                                                                                                                                                                                                                                <  1:72162/251‑1 (MQ=255)                        aTGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑GTCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGGGAAACTCACCA‑GACTGACGCCCGCCGCAATCa                                                                                                                                                                                                                                >  1:8666/1‑250 (MQ=255)                        aTGACCTCTGCGCTGCGGGTAAGCAGGAGCAGGGTAGGTAATTTTTCACTCAGCGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGAGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCATCAGCGGGGGGCTTTG‑CAGGCGGGCG‑ATAATCTCTTTTTTAAC‑AACAA‑TAATTCATGCC‑TGTCGCCCGCATAACAAAT‑TAT‑C‑GCCGGAGAAACACACCC‑AACTGAcacccacaacccacc                                                                                                                                                                                                                                >  2:330723/1‑240 (MQ=255)                           aCCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCC‑GACTGACGCCCGCCGCAATCAACTc                                                                                                                                                                                                                            >  2:37309/1‑251 (MQ=255)                           aCCTCTGAGCTTCGGGTAAGCAGGAGCAGGGCCGGTAATTTTTAACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGACGCGCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑AAGGCGGGCC‑ATCATCTCTTTTTTAAC‑AAAAA‑TAATTTCCGAC‑TGCGGGCCGCCTAAAAACT‑TGT‑A‑TCCCGAAAAACTCACCC‑GTCCGACGCCCGCCCCAATccaatc                                                                                                                                                                                                                            >  2:588112/1‑246 (MQ=255)                            ccTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCCTCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAAAT‑TGT‑C‑GCCGGAGAAAATTACCA‑TACTGACGCccgccggcatccactcc                                                                                                                                                                                                                           >  1:329102/1‑240 (MQ=255)                              tctGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACCA‑TAATTCCTGCC‑TTCGGCCCGCCCAACAACT‑TGT‑C‑GCCGGAGAAACTCACCC‑GACTGAGGCCCCCCGCAATCAACTCCtc                                                                                                                                                                                                                         >  2:458650/1‑250 (MQ=255)                               cggcgctgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGGGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GCCTGACGCCCGCCGCAATCAACTCCTg                                                                                                                                                                                                                         <  1:286105/248‑1 (MQ=255)                                        ggtaaaGCGGGCGGAGGTTCGGTATTTTTCCACTCAGAGCTTCAGGGTGTAAA‑AAAAGACACACCGGGGCTTCCAGCGCCGCGGGGGTCATTTTACCCGC‑GCGA‑‑AATGC‑AC‑GCTTCGGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTTTTTTTTACC‑AACAA‑TAATCCCTGCC‑TGCGCCCCGCCAACCAACT‑TGG‑C‑GCCGGAGAAACTCACCA‑GACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGc                                                                                                                                                                                                               <  2:110515/247‑1 (MQ=255)                                        gggTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑GTCATCTCTTTTTTACC‑CACAA‑TAATTCCTGCC‑TGCGGCACGCCTAACCACT‑TGT‑C‑GCCGGAGAAACTCACCC‑GGATGAAGCCCGCCGCAAACCACTCCTGCAGCCTTGGc                                                                                                                                                                                                               >  2:289255/1‑251 (MQ=255)                                        gggTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCCCCA‑GCCTGACGCCCGCCGCCATCCACTCCTGCGGCATTGGc                                                                                                                                                                                                               >  1:276827/1‑251 (MQ=255)                                            aggCAGGCGCGGGGTCGGTAATTTTTCACCAAGAGCTGAGGGGTGTAAA‑AAAAGACGCAACGTGGCTCCCAGGGCCGCGGGGGT‑ATTTTGTCC‑C‑GCGTTAAAGGC‑AC‑TCTCCAGCGGGGGGCTTTG‑CAGGCGGGCG‑TTCAGCCCTTTTTTACA‑AACAA‑TAATTCTTCCC‑GGCGGCCCGCCTACCAACT‑TGC‑C‑CCCGGAGAAACTCCCCA‑GACTACCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCtt                                                                                                                                                                                                           <  2:220806/249‑1 (MQ=255)                                             aGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCC‑GACTGACGCCCGCCGCAATCCACTCCTGCGGCCTTTGCTCttt                                                                                                                                                                                                          >  1:269533/1‑251 (MQ=255)                                                   cgcAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGGGAAACTCACCC‑GACTGACGCCCGCCGCAATCAACTCCCGCGGCCTTGGCTCTTTCGGCa                                                                                                                                                                                                     >  2:248736/1‑250 (MQ=255)                                                     cagggatggTAATTTTCCACCAAGAGCTTCAGGTTGTAAA‑AAAAGACGCAACGTGGCTCCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AAAAA‑TAATCCCTGCC‑TGCGGCCCGCCTAACAATT‑TGC‑C‑GCCGGAAAACCCCCCCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAc                                                                                                                                                                                                  <  1:7596/244‑1 (MQ=255)                                                     cAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AAAAA‑TAATTCCTGCC‑TGCGGGCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAAATCACCA‑GACTGAAGCCCGCCCCAATCAACTCCTGCGGCATTGGCTCTTTCGGCaaa                                                                                                                                                                                                   >  1:265519/1‑250 (MQ=255)                                                      ggggTCGGTAATTTTCCCCCCAGAGCTTCGGGGTGTAAA‑TAAAGACGCAACGTGGCTCCCAGCGCCGCGAGGGTCTTTTTATCCGC‑GCGT‑‑AATGC‑CC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTCCC‑AACCA‑TAATTCCTTCC‑TGCGGCCCGCCTAACACCT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAcc                                                                                                                                                                                                 <  1:25299/250‑1 (MQ=255)                                                      ggggTCGGTAATTTTCCCCCCAAAGCTTCAGGGTGTAAA‑TAAAGCCGCACGGTGGCTCCCAGCCCCGCGAGGGTCATTTTACCCGC‑GCGT‑‑AATGC‑CC‑GCGTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTACCC‑AACAA‑AAATCCCGCCC‑TGCGGCCCTCTTAACAACT‑‑GT‑CCGCCGGAGAAACTCCCCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAcc                                                                                                                                                                                                 <  1:247703/250‑1 (MQ=255)                                                         gttggTAATTTTTCGCCCAGGTCTCCAGGTTGAAAA‑TAAAGCCGCACCGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGTCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTCCC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAATT‑TTT‑C‑GCCGGAGAAACTCACCA‑GCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                              <  1:237486/248‑1 (MQ=255)                                                         gTCGGTAATTTTTCACTCAGAGCTGCAGGGTGTAAA‑TAAAGACGCACCGTGGCTTCCAGCGCCGCGAGGGTCATTTTACCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                              <  1:472471/251‑1 (MQ=255)                                                               aaTTTTCCACTCAGAG‑TTGCGGGTTTAAAATAAGGACGCAACGTGCCTCCCAGCCCCGCGGGGGTATTTTTACCCGC‑GCGA‑‑AACTC‑AC‑GCGTGAGCGTGTGGTTTTG‑CAGGCGGGCG‑ACCATCTCTTTTTCACA‑AACAA‑AAAGTCCTGCT‑TCGGGCCCGCCAAACATGT‑TGT‑C‑CCGGGAGAAACTCACCA‑GCCGGACGCCCGCCGCAATCAACTCCTGCGGCATTTGCTCTTTCGGCAAACCGTACTGGCt                                                                                                                                                                                        <  2:334478/251‑1 (MQ=255)                                                                 tttttCACTCAGAGCTCAAGGGTGTAAA‑AAACGACGCAACGTGCGTTCCAGCGCCGCGAGGTTCTTTTTATCCCC‑GCGT‑‑AATGC‑AC‑GCTTCGGGGGGTGGTTTTG‑CAGGCGGGCG‑ATCATCTTTTTTTTCCC‑AACAA‑TATTCCCTGCC‑TGCGGCCCGCCTACCAACT‑TGA‑C‑GCCGGAGAAACTCCCCA‑GACTGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTa                                                                                                                                                                                       <  2:153220/250‑1 (MQ=255)                                                                     ccacccAGAGCTCCGGGGTGAAAA‑TAAAGACGCAACGTGGCTCCCAGCGCCGCGAGGGTCATTTTCGCCGC‑GCGA‑‑AATGC‑AC‑GCTACAGGGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTCCC‑AACAA‑TAATCCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCCCAA‑GCCGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATc                                                                                                                                                                                  <  2:492253/246‑1 (MQ=255)                                                                              gCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCGGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑CC‑GTTTCAGCGGGTGGTTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTCCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAACCTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGa                                                                                                                                                                         <  1:177849/251‑1 (MQ=255)                                                                                 tCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCCTCTCTTTTTTAAC‑AACAA‑TAATTCCTGAC‑TGCGGCCCGCATAACAACT‑TGT‑C‑GCCCGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCCTTGGCTCTTTCGGCGAACCGTCCTCGCTAAGATCGACCAGCGAGcc                                                                                                                                                                      >  2:458542/1‑251 (MQ=255)                                                                                 tCAGGGTGAAAA‑TAAAGCCGCAACGTGGCTCCCAGCGCCGGGAGGGTCTTTTTACCCGC‑GCGT‑‑AAGCC‑AC‑GCTTCAGGGGGTGGCTGTG‑CAGGCGGGCT‑ATCATCTCTTTTTTCCC‑AAAAA‑TAATTCCTGCC‑TGCGGCCCGCTTAACAACT‑TGT‑C‑GCCGGAGAAACGCCCAA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGc                                                                                                                                                                           <  2:339332/246‑1 (MQ=255)                                                                                    gggTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCCCTTTTTTACC‑AAAAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TAT‑C‑GCCGGGGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAACGTACCGGCTAAGATCGACCAGCGAGCCAcc                                                                                                                                                                   >  2:219604/1‑250 (MQ=255)                                                                                              aaaagccgcaacgtgccgccaggcgcGGCGGGGGTCATTTTA‑CCGCCGCGT‑‑A‑TGC‑CCCGCTCCAGCGGGGGTCTTTG‑AAGGGGGGTG‑ATCA‑CTCTTTTTTACCCAACAT‑TAATTCC‑GCCGTCCGGCCCGCCGACCAACT‑GGC‑C‑GCTGGAAAAACCCGCCA‑GGCGGGCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGGACTGGCTAAGATCCACCAGCGAGCCACTGCCTAAATc                                                                                                                                                          <  2:245559/229‑1 (MQ=255)                                                                                                aagcccaagcggggCTGCCAGCCCCGCGGGGTTCTTTTTCCCCGG‑GCGT‑‑AATGC‑AC‑GTTTCGGGGGGTGGTTTGG‑CGGGCGGCCG‑TTCATTTTTTTTTTACC‑AACAA‑TATTCCCTGCC‑GGCGGCCCGCCTAACAACT‑TGC‑C‑CCCGGAGAACCTCACCG‑GACTGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAg                                                                                                                                                        <  2:8666/239‑1 (MQ=255)                                                                                                     gCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAAAAACT‑TTT‑C‑GCCGGAGAAACTCACCA‑GACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCTGCAAACCGCACTGGCTAAGATCGACCAGCGGGCCCCTGCCTAAATCAGTCACt                                                                                                                                                   >  1:80528/1‑251 (MQ=255)                                                                                                            ggCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑GGCAACTCTTTTTTACC‑AAAAA‑TAAATACTGCC‑TTCCGCCCGGCCAACAAAT‑TGT‑C‑GCCCAAAAAACGCACCA‑CACTGACGACCGACCCAATCAACTCCTGCGGCCTTGGCCCTTACGGCAAACCGTACTGGCTAAGATCGACCAGCACGCCACTGCCTAAATCaatcccgaaggga                                                                                                                                             >  1:541425/1‑238 (MQ=255)                                                                                                             gCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑GTCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAAATCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGaa                                                                                                                                           >  1:325300/1‑250 (MQ=255)                                                                                                                 ccAGCGCCGCAAGGTTCATTTTATCCGC‑GCGT‑‑AAGGC‑CC‑GCGCCGGCGGGGGTCTTGC‑CAGGCGGGCA‑ATCATCTTTTTTTTACC‑AACAA‑AAATTCTTGCC‑TGGGCCCCGCTTAACAACT‑GGT‑C‑CCCGGAGAAACCCCCCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATcc                                                                                                                                      <  1:309960/251‑1 (MQ=255)                                                                                                                  cAGCGCCGCGAGGTTCATTTTACCCGA‑GC‑T‑‑AAAGCCAC‑GCTTCTGCGGGTGGCTTTG‑CAGGCGGCCG‑ATCATCTCTTTTTTACC‑AAAAA‑AAATCCCGCCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAACCCCCCCA‑CACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCa                                                                                                                                     <  1:360195/251‑1 (MQ=255)                                                                                                                  cAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TTCGGCCCGCCTTACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCCCAATCCACTCCTGCGGCATTGGCTCTTTCGGCCAACCGTACTGGCTAAGATCCACCAGCGAGCCACCTCCTAAATCAATCACTAC‑GGGAACaacc                                                                                                                                      >  1:467292/1‑247 (MQ=255)                                                                                                                           gAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCCCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAAACAGTCACTAC‑CGGAACATCCAGCTCTTTGc                                                                                                                            >  2:425837/1‑251 (MQ=255)                                                                                                                                tCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACCACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAACGTACTGGCTAAGATCGACCAGCGAGCCCCTGCCTAAATCAGTCACTAC‑GGGAACAACCAGCTCTTTGCCGAgc                                                                                                                       >  1:489178/1‑251 (MQ=255)                                                                                                                                       ccccGC‑GCTT‑‑ATTGC‑AC‑GCTGCAGGGGGGGGTGTTG‑CGGGGGGGTG‑ATCTTCTCTTTTTCCCC‑AACAA‑AAATCCCGGCC‑TGCGGCCCGCCTAACACCT‑TGT‑C‑CCCGGAAAAACCCTCCA‑CACGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGccacc                                                                                                                 <  2:469670/248‑1 (MQ=255)                                                                                                                                       aTCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GCCCGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCCCTTTCGGCAAACCGTACTAGCTAAGATCGACCAGCCAGCCACTGCCTAAATCAGTCCCTACCGGGAA‑ATCCCGCTCTTTTCCGAGAGcccccc                                                                                                                >  2:317187/1‑247 (MQ=255)                                                                                                                                        cgcccctcgT‑‑AATAC‑TC‑GCTTCGGCGGGTGGTTTTG‑CGGGGGGGCT‑ATCATCTTTTTTTGTCC‑AACAA‑AAATCCCTCCC‑TGCGGCCCGCCAAACAACT‑TGC‑C‑GCCGGAGAAACTCCCCA‑GTCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACGGCCTAAATCAGTCACTAC‑GTGAACATCCAGCTCTTTGCCTAGCGccacc                                                                                                                 <  2:390396/243‑1 (MQ=255)                                                                                                                                             cccgcgcgaaagcctctcTTCAGCGTGTGGCTTGC‑CAGGGGGGCT‑ATC‑TCTTTTTTTTCCCTAACAT‑AAATTCCGGCC‑TGCGCCCCGCCAAAAAATT‑TGT‑C‑GCCGTAAAACCACACCA‑TTCTGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCGCTTTCGGCAAACCGTACTGGCTAATATCTACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGATCGCCACCAg                                                                                                               <  2:389875/234‑1 (MQ=255)                                                                                                                                              gcgT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATAATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑CTCGGCCCGCCTAAAAACT‑TGT‑C‑GCCGGAGGAACTCAACA‑CACTGACGACCGACGCCATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAACCTACTGGCTAAGATCGACCCGCGAGCCCATGCCTAAATCAGCCAACAC‑GGGAAAATCCAGCCCTTTGCCGAGCGCCACCAGTTccc                                                                                                          >  1:95424/1‑250 (MQ=255)                                                                                                                                               cgT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGGGAGCCACTGCCTTAATAAGTCACTAC‑GGGGACATACCGCTATTTGCCGGGCGCCCCCAGTTCCGc                                                                                                         >  1:93409/1‑251 (MQ=255)                                                                                                                                                           c‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTCCC‑AAAAA‑TAATTCCTGCC‑GGCGGCCCGCCTAAAAACT‑TGT‑C‑GCCGGAGAAACTCCCCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTAt                                                                                                <  1:103516/251‑1 (MQ=255)                                                                                                                                                              cctccggcggtggtTTTGGCAGGAGGGCG‑ACCATTTTTTTTTAACA‑AACAA‑TAATCCCTGCC‑GGCGGCCCGCCTAA‑AACTGTGA‑C‑GCCGGAGAAACAC‑CCACGCCTGCGGCCCGCCGCAATCAACTCCTGCGGCAGTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATc                                                                                               <  2:515576/243‑1 (MQ=255)                                                                                                                                                               ttCAGGGGGTGGCTTTG‑CGGGGGGGCG‑CTCATCT‑TTTTTTACC‑CACAAATAATTCCGCCC‑GGCGCCCCGCCTAAAAATT‑TGC‑G‑GCCGGAGAACCCCCCCA‑GACTAACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCg                                                                                              <  2:316423/250‑1 (MQ=255)                                                                                                                                                                tCAGCGGGTGGCTTTG‑GGGGGGGTCG‑CCCAGCTCTGTTTTACC‑AACAA‑TAATACCCGCC‑TGCGCCCCGCTTAA‑AAAT‑TGTTC‑CCCGGAGAACTTCACCA‑GACTGCCCCCCGCCGCAATCAACTCCTGCGGCATTGTCTCTTTCGGCAAACCGTACTGGCGAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCTCCAGTTCCTCTTCATCTATCg                                                                                              <  2:133920/249‑1 (MQ=255)                                                                                                                                                                        gggCTTTG‑CAGG‑GGGCGCTTCATCCCTTTTTTCCC‑AAAAA‑AAATCCGTGCC‑TCGGGCCCGCCAAACAACT‑TGC‑C‑CCCGGAAAAACCCCCCA‑GACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGaa                                                                                    <  2:152854/250‑1 (MQ=255)                                                                                                                                                                              tG‑CAGGCGGGCT‑ATCGTCTCTCTTTTACC‑CAAAC‑TAATTCCTGCC‑AGCGGCCCGCCTCACCACC‑TGT‑C‑ACCCGCGCACCTACCCA‑GAATGGCGCCAGCCTCAATCAACCCCTGCGGCATTAGCTCTTTTCGCCAACCGGACTAGCTAAGATCGAACACCGACCCACTGCCTAACTAAGTCACTAA‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCACTTCCGCTTCCTCCATCGATTTTGTGAACCCCTg                                                                              >  2:302265/1‑251 (MQ=255)                                                                                                                                                                               g‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTCCC‑TGCGGCCCGCCTAACAACT‑GGT‑C‑GCCGGAGAAACTCCCCA‑GACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTg                                                                              <  1:130629/250‑1 (MQ=255)                                                                                                                                                                                 cAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGa                                                                             >  2:515131/1‑250 (MQ=255)                                                                                                                                                                                  aGGCGGGCG‑ATCCTCTCTTTTTTACC‑AACCA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCCCCAGTTCCGCTTCCTCTATCGCTTTGGTGAACCCCTGAAt                                                                           >  2:303158/1‑251 (MQ=255)                                                                                                                                                                                      gggcg‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGcc                                                                                                                           >  1:531469/1‑199 (MQ=255)                                                                                                                                                                                      gggcg‑ATCATCTCTTTTTTACC‑AACAA‑TAATCCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTg                                                                       <  1:248736/251‑1 (MQ=255)                                                                                                                                                                                       gtcg‑ATCACCTCTTTTTTCCC‑AATAA‑TAATTCCTGCT‑GGCGGCCAGCCTATAAACT‑TGG‑C‑GCTGGATAAACCCCCCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGt                                                                      <  1:582892/249‑1 (MQ=255)                                                                                                                                                                                       ggcg‑ATCATCTCTTTTTTACA‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCCGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCCGTTCCGCTTCATCTATCGCTTTGTTGAACCCCTGAATGCTGt                                                                      >  2:52867/1‑251 (MQ=255)                                                                                                                                                                                          ccaccatcTCTTTTTAACC‑AACAA‑TAAGCCCGCCC‑AGCGCCCCGCCAAACATCT‑TGT‑C‑GCCGGAGAACCTCCCCA‑TACTGACGCCCGCCGCAATCAACGCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGGCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGGTCGGCTTCATCTATCGCTTTGGTGAACCCCTGAATTCTGTAg                                                                    <  2:95424/247‑1 (MQ=255)                                                                                                                                                                                                   tttttttCCC‑AACAA‑TAATCCCTGCC‑TGCGGCCCGCCAAACAACT‑TGC‑C‑GCCGGAGAAACTCCCCA‑GTCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTgg                                                             <  1:421327/248‑1 (MQ=255)                                                                                                                                                                                                       tttACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGt                                                        >  1:359444/1‑250 (MQ=255)                                                                                                                                                                                                           cc‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCC‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCGTCATCTATCGCTTTGGTGAACCCCTGAATGCAGTAGTTACTGGTATGTACttt                                                   >  1:3355/1‑251 (MQ=255)                                                                                                                                                                                                            c‑aacaa‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtc                                                  <  1:10901/251‑1 (MQ=255)                                                                                                                                                                                                               acaa‑TAATTCCTGCC‑TGCGGCCCGCCTACCAACT‑TGT‑C‑GCCGGAAAACCCCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtcat                                                <  1:9998/251‑1 (MQ=255)                                                                                                                                                                                                               aaaa‑taatTCCTGCG‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAACCCCCCCA‑GCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtcat                                                <  1:458650/249‑1 (MQ=255)                                                                                                                                                                                                                  a‑taatTCCTGCC‑TGCGGCCCGCCTAACACCT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTAACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTCCTTtcatca                                              >  2:362839/1‑250 (MQ=255)                                                                                                                                                                                                                      atccctgcc‑tgCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAACCTCACCA‑GCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAg                                          <  1:489695/248‑1 (MQ=255)                                                                                                                                                                                                                       ttCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGt                                         >  2:424518/1‑251 (MQ=255)                                                                                                                                                                                                                             cc‑tgcGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGATGTAGTTACTGGAATGTACTTTCATCAACAGTGCGGt                                    >  2:422659/1‑250 (MQ=255)                                                                                                                                                                                                                                cgcGGCCCGCCAAACAACT‑TGT‑C‑GCCGGAGAACCCCCCCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTAtt                                 <  1:425837/250‑1 (MQ=255)                                                                                                                                                                                                                                  cGGCCCGCATACCAACT‑TGT‑G‑CCCGGAGAAACTCACCA‑GACGGACGCCCGCCGCAATCAACTCCTTCGGCATTGGCTCTTGCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTAtttt                               <  1:294606/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttc                              >  2:564072/1‑251 (MQ=255)                                                                                                                                                                                                                                    gcccgaatAACAACT‑TGT‑C‑GCCAGAGACACTCACCA‑GACTGACGCCCGCCGCAATCACCTCCTGCGGCATTGGCTCTTTAGGCAACCCGTCCTGGCTAAGATAGACCAGCAAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTCTCGCCTTGGTGAACCCCTGCATGCTGTAGTTACTGGTATGTACTTTCATCAACAGCGCGGTATTttc                              >  2:530583/8‑250 (MQ=255)                                                                                                                                                                                                                                     cccGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACAGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGGTACGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGAATTttcat                            >  2:448523/1‑251 (MQ=255)                                                                                                                                                                                                                                               accT‑TGT‑C‑GCCGGAGAAACTCCCCA‑GACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTg                  <  1:424518/249‑1 (MQ=255)                                                                                                                                                                                                                                               aacT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTg                  >  1:111218/1‑251 (MQ=255)                                                                                                                                                                                                                                                acT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTg                  >  2:501731/1‑250 (MQ=255)                                                                                                                                                                                                                                                 cT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCACCAGAGCGGAATTTTCATTCCCCGCCTGAc                >  2:463267/1‑251 (MQ=255)                                                                                                                                                                                                                                                  t‑tGT‑C‑GCCGGAGAAACTCAAAA‑GACTGACGCCCGCCGCAATAAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGAGCGGTATTTTCATTCACCGCCTGACg               >  1:434137/1‑251 (MQ=255)                                                                                                                                                                                                                                                               aaacaCTCACCA‑GACGGACGCCCGCCGCAATCAACCCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCTCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATGTTCATGCACCGCCTGACGATAATCATTc     <  2:113967/247‑1 (MQ=255)                                                                                                                                                                                                                                                               aaaaaCTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATAATCATTc     <  1:484094/249‑1 (MQ=255)                                                                                                                                                                                                                                                                  aaCTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAACATCGACCAGCGAGCCACTGCCTCAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCCACAGTGCGGTATTTTCATTAACCGCCTGACGATAATCATTcgcg  >  1:62561/1‑251 (MQ=255)                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        GTAAACGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAA‑TAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGC‑GCGT‑‑AATGC‑AC‑GCTTCAGCGGGTGGCTTTG‑CAGGCGGGCG‑ATCATCTCTTTTTTACC‑AACAA‑TAATTCCTGCC‑TGCGGCCCGCCTAACAACT‑TGT‑C‑GCCGGAGAAACTCACCA‑GACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTAC‑GGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATAATCATTCGCG  >  NC_000913/3760136‑3760629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑