Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	252.5 / NA	68	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (29/39);  total (29/39)

TCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560209‑3560698                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    tcttGTACCATCAACGGGTGAGAAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGGGCGCTCCCGCTTCCGGCGGGGGGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                                      <  1:400082/247‑1 (MQ=255) gcttTTCCCATCACGGTGTTAGCAACGTTGTGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGTAGTGCGGGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGAAAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                                      <  1:331159/246‑1 (MQ=255)     ttACCATCCCCGTGTTGGCGACGTTGAGATTGTTGGCGACAATGCCCAAATTTCTGTGATTGAGCATTGCGTGCGCTACCCATTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGACTTGCTCCGCCGCTGTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgccggacc                                                                                                                                                                                                                                              >  2:49284/1‑243 (MQ=255)             aaCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGAAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                      >  2:148923/1‑251 (MQ=255)                   ttAGCAACGTTGCGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGc                                                                                                                                                                                                                                >  2:26222/1‑251 (MQ=255)                      gCAACGTTGAGATTGGTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                               >  2:432085/1‑249 (MQ=255)                       cAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                            >  1:443876/1‑251 (MQ=255)                       cAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                            >  1:438031/1‑251 (MQ=255)                        aactttaagaTTGTTGGTGACAAGGCGCAAGTTGTGGTGTTTAAGTAGTCCTTTCGCTACCGCTTCCGGCG‑GGTGCCCGATT‑TCGATAAACATGGTCGAGCAATGGGGGATTTGCTCCGCCACTTTGCGGGCTAGGCGCTCTTTTTCTTC‑GGTCTGGGTGGCTTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATTGCGCAGTATCAGGTTTTGCTCCGCCAGCt                                                                                                                                                                                                                           <  1:399133/244‑1 (MQ=255)                        aaCGTTGAGATGTTTGGTGACAAGGCGCAAATTGCTGTGATTGAGCAGTGCGGGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCt                                                                                                                                                                                                                           <  1:427204/251‑1 (MQ=255)                         aCGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTGGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                                          <  1:88696/251‑1 (MQ=255)                            tggagatttgtgGTGACAAGGCGCAAATTGCTGTGTTTGAGCAGTGCGTGCGCGCCCGCTTCCGGCGTGTTGCC‑GATA‑TCGAAAAACAGCGTCGAGCCATTGGGGATTTGCGCCGCCACTTTGCGGGCGTTGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                                        <  1:352213/240‑1 (MQ=255)                                   tgttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCACTACCACATCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGCGCCATTGGGGCTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGTCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                                >  1:363462/1‑251 (MQ=255)                                           aCAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                        >  1:219292/1‑251 (MQ=255)                                                     aatggttgtgATTGAGCAGTGCGTGCGCTCCGGCTTCCGGGGTGGTCCC‑GATA‑CCGATAACCAGCGCCGAGCCTTTGGGTTTTTGCTCCGCCATTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCGGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                              <  1:299914/245‑1 (MQ=255)                                                     aatagttgtgATTGAGCCGAACGTGCGATACCGCTTCTGGCATGACGCT‑GATA‑TCGATAAACAGCGTCGAGACATTGGGCATTTAATCTGCCACTTTGCGGGAGTTGCGATATCTTTATTC‑AGCCTGGGCGGCATTGCGCTCGTGCCCCGGCGTGTTCACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCCGGATCAGGTTATGCTCCGCCAGCTCATTGCGGTAACGGCGAATAGACCGCggg                                                                                                                                                                                              >  2:568998/7‑251 (MQ=255)                                                              gatggAGCAGTTCGTGCACTACCGCTTCCGTCGTGGTGCC‑GATA‑TCGAAAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                                                     <  1:165538/247‑1 (MQ=255)                                                                  gAGCAGGGCGTGCGCTACCGCTTCCGGCGGGGTGCC‑AATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                                                                                                                                 <  1:491436/251‑1 (MQ=255)                                                                   aGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                                                                                                                                >  2:224041/1‑251 (MQ=255)                                                                     cAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                                                                                                                              >  2:160053/1‑251 (MQ=255)                                                                        ggcgggcgcTCCCGCTTCCGGCGGGGTGCC‑GATA‑GCGATAAACAGCGGCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa                                                                                                                                                                           <  1:182816/246‑1 (MQ=255)                                                                         gggtgcgCTCCCGCTTCCGGGGTTG‑GGC‑GATAATTGAAAAAGAGCGTCGGGCCATTGGGTATTGGCCCCGCCGCTTTGCGGGCGATGCGCTTTTTTTCTTC‑GGTCTGGGTGGCCTTGCGAGCGTGCCAGGGGGTGTTAACCGAACGTGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACTGAGAAATGCTCTag                                                                                                                                                                          <  2:369609/249‑2 (MQ=255)                                                                          cgtgcgCTACCGCTTCCGGCGTGGTTCC‑GTTA‑TCGATAACTGGCGCCGAGCCATGGGGGATTTTCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                         <  1:157161/251‑1 (MQ=255)                                                                                cTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct                                                                                                                                                                   >  1:87137/1‑251 (MQ=255)                                                                                     gCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                              >  2:455199/1‑251 (MQ=255)                                                                                            cggcggggtcccaatttCGAAAAACAGGGTCTACCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCCCTCTTTTTCTCC‑GGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                                         <  1:365149/235‑1 (MQ=255)                                                                                             cGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt                                                                                                                                                      >  1:126306/1‑251 (MQ=255)                                                                                                      catttatggATAAACAGCGTCGAGCCCTTGGGGATTTCCTCCGCTACTTTGCGGGCGATGCGCGCTTTTTCTTC‑GGTCTGGGTGGCCTTGCTATGGTGCCACGGCTTTTTAACCGAACTGGTAGGCATCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTGGCTCCGCCAGCTCATTTATGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTGCGGTACTCACAGAGCCctg                                                                                                                                               <  1:490535/243‑1 (MQ=255)                                                                                                          a‑tCGATAAACAGCGCCGAGCCATTGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                                          <  1:160053/251‑1 (MQ=255)                                                                                                              gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                                        >  1:458256/1‑250 (MQ=255)                                                                                                                  acgagcggcggcacatgggggTTTTGCTCCGCCGTTTTGGGGGCGAGGCGCTCTTTTTCTGC‑GGTGTGGGGGGCCTTGCGATCTGGCCACGGCGTGTTAACCGAACGGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                                   <  2:528393/234‑1 (MQ=255)                                                                                                                      gggTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTCC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                                                                                                               <  1:236339/249‑1 (MQ=255)                                                                                                                                aTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt                                                                                                                     >  1:563663/1‑251 (MQ=255)                                                                                                                                 ggggggtgttGCCCCGCCATTTTGGGGGGGATGCGCTCTTTTCCGTC‑GGTCTGGGTGGCCTTGCGAGCGGGCCACGGCGTGTTACCCGAGCGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                                    <  2:162050/243‑1 (MQ=255)                                                                                                                                   ggggATTGCCTCCGCCACTTTGCGGGCGAGGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                                  <  1:246013/251‑1 (MQ=255)                                                                                                                                    gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                                                                                                                 >  1:67062/1‑251 (MQ=255)                                                                                                                                     ggttttGCGCCGCCTCTTTGCGGGTGTTGCGCTCGTTTTCGTC‑GGTTTGGGTGGCATGGCGTTCGTGCCACGGCGGTTTAACCAAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCATTTCGATAATACCGTTGTGa                                                                                                                <  2:297281/248‑1 (MQ=255)                                                                                                                                        tttGCTCCGCCTCTTTGCGGGCTAGGCTCTCTTTTTCTTC‑GGTCTGGGTGGTCTTGCGATCGTGCCACGGCGTGTTACCCGACCTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGATAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                              <  1:91816/250‑1 (MQ=255)                                                                                                                                          gtacccGCCACTTTGGGGGCGATGCGCTCTTTTTCTGC‑GGTCGGGGTGGCCTTGCGATCGTGCCTCGGCGTGTGAACCGAACGGGAAGGCGGCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCGGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                           <  2:443244/247‑1 (MQ=255)                                                                                                                                              ccgcccattggCGGGCGAGGCGCT‑GTTTTCTTCCGGTCTGGGTGGCCGTGCGATCGTGCCAGGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGATGtgtt                                                                                                       <  2:237364/241‑1 (MQ=255)                                                                                                                                              ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                                       <  1:455199/251‑1 (MQ=255)                                                                                                                                                    cTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                                                 >  1:226185/1‑251 (MQ=255)                                                                                                                                                       tGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTCACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                              >  2:543816/1‑251 (MQ=255)                                                                                                                                                        gCGGGCGATGCGCTCTTTTCCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                             <  1:110839/251‑1 (MQ=255)                                                                                                                                                           ggCGATGCGCTCTTTTTCTTC‑GGTCTGGGGGGCCTCGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtata                                                                                          <  1:49284/251‑1 (MQ=255)                                                                                                                                                            gCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                                                         <  1:499272/251‑1 (MQ=255)                                                                                                                                                             cGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaaa                                                                                        <  1:388474/251‑1 (MQ=255)                                                                                                                                                               aTGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATc                                                                                      >  1:122971/1‑251 (MQ=255)                                                                                                                                                                  gccgctctcccgacctgTCTGGGTGCCCTTGCGACCGTGCCACGGGGTGTTACCCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATccc                                                                                    <  2:131022/235‑1 (MQ=255)                                                                                                                                                                  gattttttttCTTC‑GGTCTGGGTGGCCTGGCGTTCGTGCAACGGCGTGTTAACCGACGTGGAAGGCAGCGCCGTACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATCCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                                   <  1:35557/246‑1 (MQ=255)                                                                                                                                                                  cgcTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                                   >  1:482846/1‑251 (MQ=255)                                                                                                                                                                       tttttCTTC‑GGTCTGGGTGTCCTTGCGCACGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGACGCACCGCCCTGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAATCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCAATAACCATGCTGTTTAACCATTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATACATctct                                                                                   >  2:35557/1‑243 (MQ=255)                                                                                                                                                                               gcgtgcggggTGGCCTGGCGACCGTGCCACGGCGTGGTAACCGAACGGGAAGGCGGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttc                                                                       <  2:425070/244‑1 (MQ=255)                                                                                                                                                                                gggTCGGGGTGGCCTGGCGACGGTGCCACGGCGGGTTAACATAACTGGAAGGCAGCGCCGCTCCGCCAGGAGGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcg                                                                      <  2:134739/250‑1 (MQ=255)                                                                                                                                                                                  gtcgggggggCCTGGCGACCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtt                                                                    <  1:432085/243‑1 (MQ=255)                                                                                                                                                                                  gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATccc                                                                                    >  1:131022/1‑235 (MQ=255)                                                                                                                                                                                    cggggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttt                                                                   <  2:67062/248‑1 (MQ=255)                                                                                                                                                                                           gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAt                                                           >  1:229297/1‑251 (MQ=255)                                                                                                                                                                                            ccTTGCGAGCGGCCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAtt                                                          <  2:435976/251‑1 (MQ=255)                                                                                                                                                                                             cTTGCGATAGTGCCACGTCGTATTGACCGAACTCGAAGGCCGCGCCGCACAGCAATGATGGCGAAGGATCAGGTTTTGCTCCGACCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAACCTGCTGTTTAACCAGTTAGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATACATGGAATTATTTTCGTTTTCGCGCATTg                                                         >  2:336545/1‑251 (MQ=255)                                                                                                                                                                                                          cACGGCGTGTTAACCGACCTGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa                                            <  1:461904/251‑1 (MQ=255)                                                                                                                                                                                                             ggCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                           >  1:482607/1‑249 (MQ=255)                                                                                                                                                                                                                 gtttAACCGAACTGGAGGGCTGGGCCGCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATATCCCTGCTGTTTAACCAGGTCGATAATACCGTTGTGACTTTGTGTTGGTGTCATTTATAAATCCCTGGAATTATTTTCGTTGTCGCGCGTTGTGCGAATCAACAGAAGCCAt                                     <  2:524962/249‑1 (MQ=255)                                                                                                                                                                                                                   ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                                   >  1:164315/1‑251 (MQ=255)                                                                                                                                                                                                                    tacccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTGTCGCGCATTGAGCGAATCAACAAAAGCCATCg                                   <  1:149507/247‑1 (MQ=255)                                                                                                                                                                                                                           aCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTGCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTGGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAccc                           <  1:301624/251‑1 (MQ=255)                                                                                                                                                                                                                             gggagggCAGCGCCGCACCGCCATGATGGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAc                         <  2:533498/246‑1 (MQ=255)                                                                                                                                                                                                                              ggAAGGCAGCGCCGCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCa                          <  2:340953/249‑1 (MQ=255)                                                                                                                                                                                                                                     aGCGCCGCACCGCCATGATGCCGCAGGATCAGGTTTTGCTCCGCCAGATCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAACAGCCATCGCTAAACCCACGGCTAAcc                 >  1:225927/1‑251 (MQ=255)                                                                                                                                                                                                                                                gCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCCGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgt      >  1:289379/1‑251 (MQ=255)                                                                                                                                                                                                                                                    tgatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTc  >  1:232559/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    TCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCC‑GATA‑TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTC‑GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560209‑3560698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑