Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,414	C→T	G15S (GGT→AGT)	ydaG ←	uncharacterized protein YdaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,414	0	C	T	100.0%	105.3 / NA	32	G15S (GGT→AGT)	ydaG	uncharacterized protein YdaG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

CAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATA  >  NC_000913/1419177‑1419656                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   cAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACTTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCTATTTTTTGCATTTTGCGAAGCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATc                                                                                                                                                                                                                                       <  1:341322/251‑1 (MQ=255)          gcgcgcACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTg                                                                                                                                                                                                                               >  1:487756/1‑250 (MQ=255)           cgcgcACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGTTTGTAAGTGATACTGCAACAATCCATCAAATACTGaa                                                                                                                                                                                                                             <  2:543649/251‑1 (MQ=255)              gcACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAact                                                                                                                                                                                                                           >  2:112370/1‑250 (MQ=255)                 ccAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACtt                                                                                                                                                                                                                       <  2:151155/251‑1 (MQ=255)                     tttCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCTCCTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGACCTACTTCGt                                                                                                                                                                                                                    >  1:283789/1‑250 (MQ=255)                        cGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATg                                                                                                                                                                                                                <  1:322924/251‑1 (MQ=255)                           ttttAAATGTTTTGCTTTTGACCTACGTAATGGCAGTGAACTTTCCATCCTTGTTTGGGAACACGCCGCACACCATGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTCCTTCGTAATGCAc                                                                                                                                                                                                             >  2:105313/1‑251 (MQ=255)                            tttAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCAcc                                                                                                                                                                                                            >  1:15364/1‑251 (MQ=255)                             ttAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCAcc                                                                                                                                                                                                            >  1:341000/1‑250 (MQ=255)                                   gTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTa                                                                                                                                                                                                      <  2:487756/250‑1 (MQ=255)                                                         tGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTgg                                                                                                                                                                               >  1:288701/1‑251 (MQ=255)                                                          ggCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTgg                                                                                                                                                                               <  1:341846/250‑1 (MQ=255)                                                             aGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACGTCGTAATGCGCCAGCTTATCTCTCCCCTTAACGCCGGTTTGCGGa                                                                                                                                                                           <  2:339665/251‑1 (MQ=255)                                                                gAACGTTCCATCCTGGTTGGGGAATACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTGTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGGTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAAGCCATCAAATACGGAACTTCGTCGTAATGCACCATCTTATCTCTCCCGTTAACTCCGGTTGGCTGaact                                                                                                                                                                        <  2:85132/251‑1 (MQ=255)                                                                                            ccgcACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATccc                                                                                                                                            <  1:468227/251‑1 (MQ=255)                                                                                                                              ttttttGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACtt                                                                                                          >  2:446878/1‑251 (MQ=255)                                                                                                                                ttttGCATGTTGCGAATCTCACATCTTTTTGTTACGTATAGCGCTTTCTGC‑TGTCCAGAGATCCCAGTCGTTACTGCGTAAAGCCTGCACAGCCTTGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTCCTTCGTAATGCACCATCTTACCGCTCCCCTTAACGCCTGTTGGCGGAATTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACtaa                                                                                                         <  2:3651/250‑3 (MQ=255)                                                                                                                                   tGCATTTTGCGAATCTCGCATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTGGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTGATCGCCCCCCTTAACGCCGGGTGGCGGAACTAACTGCGGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAAcc                                                                                                      <  1:289348/250‑1 (MQ=255)                                                                                                                                        tttGCGGATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTAc                                                                                                >  2:97024/1‑251 (MQ=255)                                                                                                                                         ttGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTAcc                                                                                               >  1:281312/1‑251 (MQ=255)                                                                                                                                          tGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTAcc                                                                                               >  2:212139/1‑250 (MQ=255)                                                                                                                                                          tgttgCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTc                                                                                <  1:137595/249‑1 (MQ=255)                                                                                                                                                           gttgCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCAGCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGaa                                                                             <  2:341000/251‑1 (MQ=255)                                                                                                                                                                       aGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCgg                                                                  <  2:207982/250‑1 (MQ=255)                                                                                                                                                                          gACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGtt                                                              >  1:106092/1‑251 (MQ=255)                                                                                                                                                                              tCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCt                                                            <  1:446878/249‑1 (MQ=255)                                                                                                                                                                                               cacattCGTTGATGCGTAAAGCCTGCAAAGCTGGGGTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTAATTCGTAATGCATCCTCTTATCTCTCCCCTGAACGCCGGGTGGCGGAATTAACTGCTGCACTGCTAAATTTGAATCCCGCCGTCATGGTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTGCTGGGTGGCGGggtgggtg                                           <  2:414232/245‑1 (MQ=255)                                                                                                                                                                                                      gTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTg                                   <  1:112370/251‑1 (MQ=255)                                                                                                                                                                                                                            gCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAatt             >  1:489407/1‑251 (MQ=255)                                                                                                                                                                                                                                ggTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGCGGCGAAACTAACTGCTCCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAattga           <  1:92967/249‑1 (MQ=255)                                                                                                                                                                                                                                       aGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGGGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATa  <  1:212139/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   CAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTG‑CCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATA  >  NC_000913/1419177‑1419656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑