Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,569	C→T	R277W (CGG→TGG)	abgR →	putative LysR‑type DNA‑binding transcriptional regulator AbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,569	0	C	T	100.0%	88.8 / NA	27	R277W (CGG→TGG)	abgR	putative LysR‑type DNA‑binding transcriptional regulator AbgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/14);  total (13/14)

CTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAA  >  NC_000913/1405322‑1405814                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      cTGGACAATGCCGACGCCACACGGCAGCTACTACACACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAgtggc                                                                                                                                                                                                                                                    >  2:377401/1‑251 (MQ=255)   ggACAATGTCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAgtggc                                                                                                                                                                                                                                                    <  1:398708/249‑1 (MQ=255)                   acacGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGacac                                                                                                                                                                                                                                  >  1:458383/1‑251 (MQ=255)                                                      ttGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTACCCGCGTCATTAATCACGCAATTACGGCg                                                                                                                                                                                                >  2:316727/1‑250 (MQ=255)                                                              cAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCgg                                                                                                                                                                                       <  1:549365/251‑1 (MQ=255)                                                                    cAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCt                                                                                                                                                                                 >  1:493269/1‑251 (MQ=255)                                                                         gCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCaaa                                                                                                                                                                            <  1:424587/251‑1 (MQ=255)                                                                         gCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCaaa                                                                                                                                                                            <  1:90611/251‑1 (MQ=255)                                                                             caGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTTATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTAACTGCAAAGTTa                                                                                                                                                                        <  2:345369/251‑1 (MQ=255)                                                                                    gtgtAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGc                                                                                                                                                                 <  2:521904/251‑1 (MQ=255)                                                                                    gtgtAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGc                                                                                                                                                                 >  2:155768/1‑251 (MQ=255)                                                                                          tCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAACGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATaaa                                                                                                                                                            >  2:436230/1‑250 (MQ=255)                                                                                                    gTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATACCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTc                                                                                                                                                 <  2:13246/251‑1 (MQ=255)                                                                                                           gCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGAc                                                                                                                                          >  1:585687/1‑251 (MQ=255)                                                                                                               gTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCCCGAACAGGGGCTGGTGATGTTGCCGGTTAGCGCAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATcc                                                                                                                                      >  1:546203/1‑251 (MQ=255)                                                                                                               gTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATcc                                                                                                                                      <  2:250975/251‑1 (MQ=255)                                                                                                                          gtggCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATaaa                                                                                                                                                            <  1:2683/218‑1 (MQ=255)                                                                                                                          gtggCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTa                                                                                                                           <  2:190145/251‑1 (MQ=255)                                                                                                                          gtggCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGGGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATaaa                                                                                                                                                            >  2:2683/1‑218 (MQ=255)                                                                                                                                          ttCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACAcagcag                                                                                                            <  1:243104/250‑1 (MQ=255)                                                                                                                                               aGCAAACTGCCTGAAGAAATGGGCTGCGATCCCGTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTGTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATc                                                                                                      <  1:377401/251‑1 (MQ=255)                                                                                                                                                  aaaCTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACCCAGCAGTGCATCcgc                                                                                                   <  2:141063/251‑1 (MQ=255)                                                                                                                                                                           aTCCCTTGCACGAACAGGGGCTGGTGATGTTGCAGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCATCATTAATCACGCAATTCGGGCGAGAATGCGGCTATCTGCAAAGTTAAAAAAGCATAAAACAATAGAGTCTGTCGCCATCCGCCAGACTCTACAGTACACACAGCAGTGCACCCGCTTATTAAAGCCGGcctttgctcctc                                                                          >  2:587754/1‑240 (MQ=255)                                                                                                                                                                                                  gTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCCCTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAAc                                                   >  2:499738/1‑251 (MQ=255)                                                                                                                                                                                                              gTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGGCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCGCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCtt                                       <  2:585687/251‑1 (MQ=255)                                                                                                                                                                                                                                  aGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTttcttc                     >  1:427455/1‑249 (MQ=255)                                                                                                                                                                                                                                     ggCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCAATAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGGCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGTTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGa                <  2:460338/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aTTCAGTGGCGTGATAGTCGCAAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTTTTCAGGGACAGATTTCACGa    >  2:27434/1‑250 (MQ=255)                                                                                                                                                                                                                                                    tCAGTGGCGGGATAGTGGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATATGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGCCGAAATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTTCGACGGTGAATGTGTTATAATGGGTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACTGATTTCTCGaaa  >  2:316566/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAA  >  NC_000913/1405322‑1405814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑