Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,069	C→T	G153D (GGC→GAC)	fnr ←	DNA‑binding transcriptional dual regulator FNR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,069	0	C	T	100.0%	158.2 / NA	49	G153D (GGC→GAC)	fnr	DNA‑binding transcriptional dual regulator FNR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

CGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGG  >  NC_000913/1398827‑1399314                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      cGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATGTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGAtt                                                                                                                                                                                                                                               <  2:508038/251‑1 (MQ=255)  gATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGGCGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGGCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGAttt                                                                                                                                                                                                                                              <  1:193820/251‑1 (MQ=255)   aTGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTc                                                                                                                                                                                                                                             <  2:60475/251‑1 (MQ=255)   aTGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTc                                                                                                                                                                                                                                             >  2:285624/1‑251 (MQ=255)   aTGGTGATGTATTTACCTTGGACTGCCAGCATGCCGCTTTTCTGGAAGCTACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCAACGTCAGGCGGAATTCACGATGGGAGAAGCCGCGTGGGGCAAAACGACTGGACAGGTTGTAGAGGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGAGCATGTCCTTATCGTCTTTGATTTc                                                                                                                                                                                                                                             <  2:194721/251‑1 (MQ=255)       tGATGTATTTCCCTTTGACTGCCAGAATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGCCAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCg                                                                                                                                                                                                                                         >  2:548774/1‑251 (MQ=255)        gATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGc                                                                                                                                                                                                                                        >  1:144751/1‑251 (MQ=255)         aTGTATTTACCGTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATTATGTCCTGATCGTCTTTGATTTCTCCGCt                                                                                                                                                                                                                                       <  1:496211/251‑1 (MQ=255)           gTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCtc                                                                                                                                                                                                                                      >  1:271226/1‑250 (MQ=255)                           gCCAGCATGCCGCTTTTCTGGAAGCGGCCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGAGAGTTACCGATATCGCCACGAGTCATCGCCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACTACGGGACAGGTTGTAGATGAATGCATCTAGACGTTCCTCGGCATTGTTCTTCGACAACAGCAGGAGCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCatcatc                                                                                                                                                                                                                       <  1:554675/249‑1 (MQ=255)                                cATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGAc                                                                                                                                                                                                                <  1:500205/251‑1 (MQ=255)                                     cGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGc                                                                                                                                                                                                              >  1:486682/1‑248 (MQ=255)                                         tttCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTc                                                                                                                                                                                                        <  1:356499/250‑1 (MQ=255)                                         tttCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCg                                                                                                                                                                                                       >  1:93809/1‑251 (MQ=255)                                                    gACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTAACGATATCGCCACGAGTCATCGTCAGGCGGCATTCACGCGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCCTCTGCTGACGCAGATTCGGCATTTTACCg                                                                                                                                                                                            >  1:339100/1‑251 (MQ=255)                                                             gACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCg                                                                                                                                                                                   >  1:187029/1‑251 (MQ=255)                                                                    gATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTGCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAACCGTCCAg                                                                                                                                                                              <  1:93945/249‑1 (MQ=255)                                                                       ggTTTCTCCCGTCAGGCACGGATAGTTACCGATAGCGGCACGAGTCATCGTCAGGCGGAATTCACGAGGGTAGAAGCCGCGTTGGGCAAAACGACGGTACAGGTTGTAGATGAATGCAGCCATACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCGGATCGTCTTTGATTTCACCGCTCATCAGACTCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGttt                                                                                                                                                                         <  2:354231/251‑1 (MQ=255)                                                                        gTTTCTACCGTTAGGCCCATATAAGTAGTGATACCGCCACGAGTCATCGTCAGGCGGAATTCACAAGGGGAGAAGCCGCGTTTGGCAAAACGACTGGACAGGTTTTAGATGTATGCAGCCTGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCTGGGCGTGATCGTCTTGGATTTCTTCGGTCAGCAGATGCAGCATCTGCTGACACAGAGTGGGCAtttgatcttgcatttcggccagcgtctc                                                                                                                                                                        <  2:53745/251‑26 (MQ=255)                                                                          ttCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTGTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCg                                                                                                                                                                       <  1:389736/250‑1 (MQ=255)                                                                           tCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGa                                                                                                                                                                      >  1:284806/1‑250 (MQ=255)                                                                                         cAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATa                                                                                                                                                       <  1:28905/251‑1 (MQ=255)                                                                                              aGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCGCATCAGACGCATCATCTGCTGACGCAGATTCGTCATTTTACCGGACAAATCGGTCAGCGTTGCGAACGGGATTTCTCATGCCATc                                                                                                                                                  <  2:339100/251‑1 (MQ=255)                                                                                                  aCCGATATCGCCACAAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAACCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAgg                                                                                                                                              >  1:254915/1‑251 (MQ=255)                                                                                                   ccGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCCGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGCTCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGCTTCGGCATTTTACCGGACACATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGt                                                                                                                                             >  1:290299/1‑251 (MQ=255)                                                                                                   ccGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGACGCGTTGGGCAAAACGCCGGGACAGGTTGTAGATGCATGCAGCCAGACGTTACTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGCTCGTCTTTGATTTCACCGCTCCTCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAACTCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGt                                                                                                                                             >  1:561750/1‑251 (MQ=255)                                                                                                                    cATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGcgcg                                                                                                                             >  1:423402/1‑250 (MQ=255)                                                                                                                        gTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCGCCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGTGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                                                                                        <  1:218405/251‑1 (MQ=255)                                                                                                                           aGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCgg                                                                                                                     >  1:96615/1‑251 (MQ=255)                                                                                                                                   ttCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCATCGCCTGCGCGAAGCTCGGGTGATGGc                                                                                                             >  2:415123/1‑251 (MQ=255)                                                                                                                                          ggggAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGcc                                                                                                      <  1:285624/251‑1 (MQ=255)                                                                                                                                                   ccGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                                                                                             >  2:436390/1‑251 (MQ=255)                                                                                                                                                              aaaaCGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCccacc                                                                                   >  1:245403/1‑250 (MQ=255)                                                                                                                                                                     cgGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGcc                                                                           >  1:310083/1‑251 (MQ=255)                                                                                                                                                                          cAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTa                                                                      <  1:453115/251‑1 (MQ=255)                                                                                                                                                                                  aGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATCCCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGaa                                                               <  2:332578/250‑1 (MQ=255)                                                                                                                                                                                                    gTTCCGCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCtcgtcg                                            <  2:171488/251‑1 (MQ=255)                                                                                                                                                                                                           ggCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTGCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCt                                     <  2:90985/251‑1 (MQ=255)                                                                                                                                                                                                             cATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCa                                   <  1:354910/251‑1 (MQ=255)                                                                                                                                                                                                              aTTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTc                                    >  2:229776/1‑249 (MQ=255)                                                                                                                                                                                                              aTTTTTCTTCCACAACATCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCCCCATCTGCTACCGCAGATTCGGCATTTTACCGGACAACTCGTCCAGCGTTTCGCACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGAGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTACATGGAACCCAGTGCTTTGCTCGTCGCCTTGCTc                                    >  2:283077/1‑249 (MQ=255)                                                                                                                                                                                                                 tttCTTCGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTg                                >  1:340658/1‑250 (MQ=255)                                                                                                                                                                                                                    cttcGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATgg                            >  2:385248/1‑251 (MQ=255)                                                                                                                                                                                                                      tcGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATg                             <  2:93809/248‑1 (MQ=255)                                                                                                                                                                                                                       cGACAACAGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGta                          <  2:187029/250‑1 (MQ=255)                                                                                                                                                                                                                         acaacaGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGtata                        >  1:200267/1‑250 (MQ=255)                                                                                                                                                                                                                            acaGCAGGATCATGTCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACtt                    >  1:365439/1‑251 (MQ=255)                                                                                                                                                                                                                                          tCCTGATCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGACCAGCGTTTCGAACGTGTTTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGGGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCCCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCggg      >  1:261702/1‑250 (MQ=255)                                                                                                                                                                                                                                               aTCGTCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCgg  >  2:527575/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      CGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGG  >  NC_000913/1398827‑1399314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑